Dengqiang Wang

Chinese Academy of Fishery Sciences

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i-10 index

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Daqing Chen

Chinese Academy of Fishery Sciences

Xinbin Duan

Chinese Academy of Fishery Sciences

Shaoping Liu

Guangxi Medical University

Qiwei Wei

Chinese Academy of Fishery Sciences

Huiwu Tian

Chinese Academy of Fishery Sciences

Xiang-Yang Jia

Xianning Central Hospital

Xiaoni Gan

Institute of Hydrobiology

Shunping He

Chinese Academy of Sciences

Guiwei Zou

Chinese Academy of Fishery Sciences

Wei Qiwei

Qiannan Normal College For Nationalities

Cooperative Institutions

Chinese Academy of Fishery Sciences

Chinese Academy of Sciences

Ministry of Agriculture and Rural Affairs

University of Chinese Academy of Sciences

Huazhong Agricultural University

Southwest University

Institute of Hydrobiology

Ocean University of China

Zhejiang University

Peking University

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The mitogenome ofLeptobotia microphthalma(Teleostei, Cypriniformes: Cobitidae)

Mitochondrial DNA (2013)

Huiwu Tian Dengqiang Wang Xiang-Yang Jia Xinbin Duan Daqing Chen

The Leptobotia microphthalma belongs to family Cobitidae, which is endemic to the upper reaches of the Yangtze River in China. In this studying, the complete mitochondrial genome sequence of L. microphthalma has been obtained with PCR, which contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a non-coding control region with the total length of 16,512 bp. The gene arrangement and composition are similar to that of other vertebrates. Most of the genes are encoded on heavy strand, except for eight tRNA and ND6 genes. The complete mitochondrial genome sequence of L. microphthalma would contribute to better understand population genetics and protect its genetic diversity.

Cobitidae

Cypriniformes

Heavy strand

10.3109/19401736.2013.800495

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Citations (6)

Improving the Quality of Network Education for Automobile Majors in Higher Vocational Colleges under the Novel Corona Virus Epidemic

Rui Wang Jingfeng Xu Dengqiang Wang Yuming Dou

Corona (planetary geology)

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Polymorphism and evolution of MHC Class II B grass carp (Ctenopharyngodon idella) populations of Yangtze River

Freshwater Fisheries (2010)

Dengqiang Wang

Grass carp

Yangtze river

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Citations (2)

Strong Genetic Differentiation of <i>Glytothorax zanaensis</i> (Wu et al. 1981) in Nu River, China

Agricultural Sciences (2014)

Xiping Yuan Dengqiang Wang Shaoping Liu Li Hong Ke Wang

The Nu River Project comprised of 13-dam cascade is one of the biggest hydropower development projects yet conceived in China. However, hydropower development will inevitably result in the local ecological environment and biological material impact, particularly the effects on fish. Glyptothorax zainaensis is weak in swimming with suction cup and is extremely sensitive in the hydrological characteristics and water quality changes, so that it can be considered as a water quality monitor of indicator organism. Currently, with the need for conservation, fishery restoration and so on, there is a great demand for genetic information on Glyptothorax zainaensis. Fourty microsatellite loci were isolated, characterized and evaluated from Glyptothorax zainaensis. A total of 91 different alleles were observed in the 303 wild Glyptothorax zainaensis, and the percentage of polymorphic bands ranged from 41.8% to 66.7%, with an average of 90.1%. The genetic diversity value was significantly huge with the expected heterozygosity ranging from 0.578 to 0.706, the observed heterozygosity value ranging from 0.923 to 0.980 and PIC value ranging from 0.418 to 0.644, which showed that the wild Glyptothorax zainaensis population had a high level of genetic diversity. Analysis of molecular variance (AMOVA) and pairwise Fst showed a significant level of genetic differentiation (P

10.4236/as.2014.56050

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Design of remote monitoring system for new energy vehicles from the perspective of key technologies

2022 IEEE International Conference on Advances in Electrical Engineering and Computer Applications (AEECA) (2021)

Rui Wang Dengqiang Wang Wang Lan-hong Yuming Dou

The development of new energy vehicles is in a process of technology accumulation and industry formation. Vehicle enterprises and key parts enterprises are accumulating relevant information in order to improve their capabilities in the fields of technology, after-sales and service. In this paper, the remote monitoring system for electric vehicles is designed from the perspective of key technologies, which consists of two parts: the vehicle terminal and the remote monitoring platform. Each monitored electric vehicle is equipped with an on-board terminal, which CAN acquire the data of electric vehicle running status, battery voltage, current, temperature, SOC, etc. A server cluster model of data acquisition and monitoring center based on C/S and B/S hybrid architecture is proposed. This scheme can improve the real-time performance and communication reliability of the system, and has a good architecture design, which can improve the scalability and software portability of the system. It can also be used as a reference for remote monitoring systems of other devices.

Software portability

10.1109/aeeca52519.2021.9574236

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Citations (4)

Construction of a cDNA library and cloning of the MHC class I gene in silver carp (Hypophthalmichthys molitrix) infected with bacterial septicemia

Journal of Fishery Sciences of China (2013)

Dengqiang Wang Xiaosong Luo Daqing Chen

Cloning (programming)

Hypophthalmichthys

10.3724/sp.j.1118.2011.01368

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Isolation and characterization of twenty-six microsatellite loci for the tetraploid fish Dabry’s sturgeon (Acipenser dabryanus)

Conservation Genetics Resources (2012)

Shuhuan Zhang Hui Luo Hao Du Dengqiang Wang Qiwei Wei

10.1007/s12686-012-9815-2

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Citations (16)

Species diversity of drifting fish eggs in the Yangtze River using molecular identification

PeerJ (2018)

Mingdian Liu Dengqiang Wang Lei Gao Huiwu Tian Shaoping Liu

The dam constructions greatly changed the hydrologic conditions in the Yangtze River, and then significantly affected the spawning activities of indigenous river fish. Monitoring the species composition of drifting eggs during spawning season is important for protection issues. In this study, we have sampled drifting fish eggs in nine locations from 2014 to 2016. Eggs were identified using the mitochondrial cyt b gene sequence. A total of 7,933 fish eggs were sequenced successfully and blasted into the NCBI database. Thirty-nine fish species were identified, and were assigned to four families and two orders. Approximately 64% of the species identified, and 67% of the eggs, were classified in the Family Cyprinidae. Abundance and Shannon-Wiener diversity index of species were higher in the main river than in tributaries of the river. However, tributaries may be important spawning grounds for some fish species. The Jaccard's similarity index and river-way distances among sampled stations were negatively correlated suggesting the environment shapes species composition in the sampled spawning grounds. These results showed that mitochondrial DNA sequence is a powerful and effective tool for fish egg identification in Yangtze River and these data are useful for conservation efforts.

Yangtze river

Jaccard index

Main river

10.7717/peerj.5807

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Citations (20)

Development of 28 EST‐SSR markers based on transcriptome sequences of Gobiobotia filifer and cross‐species amplification

Journal of Applied Ichthyology (2019)

Weiwei Dong Huiwu Tian Dengqiang Wang Lixiong Yu Xinbin Duan

Gobiobotia filifer is a small benthic fish distributed in Yangtze River Basin. The abundance of G. filifer increased after impoundment of Xiluodu Dam and Xiangjiaba Dam. The state of population structure and changes of genetic diversity before and after impoundment of Xiluodu Dam and Xiangjiaba Dam were interesting issues. However, efficient molecular markers were rare, which will limit us to solve above problems. Twenty-eight expressed sequence tag SSRs (EST-SSRs) were successfully identified and verified as stable amplification and polymorphic loci by polyacrylamide gel electrophoresis (PAGE) and capillary electrophoresis. The number of alleles at these EST-SSR loci ranged from 3 to 14, the polymorphism information content values were 0.125–0.897, and the observed and expected heterozygosities were 0.0–0.857 and 0.132–0.928, respectively. Cross-species amplification of the 28 loci developed in this study was examined in seven individuals of each of the 7 taxa. The amplification efficiency of 28 EST-SSRs primer pairs is related to the distance of genetic relationship between cross-species with G. filifer, and same subfamily species (Xenophysogobio boulengeri and Xenophysogobio nudicorpa) showed the highest (50%) amplification efficiency. These EST-SSR markers could be used to analyse genetic diversity and population structure of G. filifer and related species.

Primer (cosmetics)

Subfamily

10.1111/jai.13969

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Citations (1)

Genetic diversity and differentiation between populations of <I>Glyptothorax zanaensis</I> in the middle and lower reaches of the Nujiang River

Hereditas (Beijing) (2010)

Shaoping Liu Ke Wang Xiping Yuan Dengqiang Wang Xingjian Yue

The development of hydroelectricity in the Nujiang River would have adverse impacts on the populations of Glyptothorax zanaensis. In order to assess the genetic diversity and differentiation of this species, we sequenced the cytochrome b gene of the mitochondria in 102 individuals of the fish collected from 6 sampling sites (Gongshan, Gudeng and Lushui in the Nujiang Prefectural District and Daojie, Mengnuo and Mucheng in the Baoshan Municipal District). A total of 87 variation sites were detected in the fragment of 1 137 bp in length, with which the 102 samples were defined as 36 haplotypes. The haplotype diversity (h) and the nucleotide diversity (pi) of total samples were 0.851+/-0.028 and 0.01356+/-0.0008, respectively. Therefore, the genetic diversity of G. zanaensis was relatively low. However, the genetic diversity of the Nujiang population was significantly higher than that of the Baoshan population. The pairwise Fst value between the populations (0.475-0.846) was higher than that within the population (0.002-0.108), which implied that the Fst value was positively related to geographic distance. Analysis of molecular variance (AMOVA) showed that the genetic differentiation between the populations and within the populations were 53.65% and 46.35%, respectively. The fixation index (Fst value) was 0.5365, indicating that there existed significant differentiation between the Nujiang population and the Baoshan population. The phylogentic tree and networks of the haplotypes of G. zanaensis showed that there were two separate lineages: the Nujiang lineage and the Baoshan lineage. Each lineage represents at least one separated management unit, or belongs to an evolutionary significant unit. It was suggested that in the construction of hydroelectric projects the measures for protecting G. zanaensis should be adopted in fully considering the populations of G. zanaensis and the status quo of their population structure to avoid the occurrence of gene exchange among populations.

Nucleotide diversity

Fixation index

Lineage (genetic)

Genetic distance

10.3724/sp.j.1005.2010.00254

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Citations (4)