Junsuke Shirai

Tokyo University of Agriculture and Technology

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Makoto Nagai

Azabu University

Tetsuya Mizutani

Tokyo University of Agriculture and Technology

Tsutomu Omatsu

Tokyo University of Agriculture and Technology

Yukie Katayama

Tokyo University of Agriculture and Technology

Mami Oba

Tokyo University of Agriculture and Technology

Tetsuya Furuya

Tokyo University of Agriculture and Technology

Kaori Sano

Icahn School of Medicine at Mount Sinai

Yuki Naoi

Kawamura Hospital

Hiroshi Aoki

Teikoku Seiyaku (Japan)

Tsuneyuki Masuda

Okayama University of Science

Cooperative Institutions

Tokyo University of Agriculture and Technology

National Institute of Animal Health

The University of Tokyo

Hokkaido University

National Agriculture and Food Research Organization

Gifu University

Suzuki (Japan)

Nippon Veterinary and Life Science University

National Institute of Infectious Diseases

Nagoya University

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Viruses produced from complementary DNA of virulent and avirulent strains of swine vesicular disease viruses retain the in vivo and in vitro characteristics of the parental strain

Archives of Virology (1998)

Toru Kanno Toru Inoue Donald R. Mackay Paul Kitching Shigeo Yamaguchi

Antigenicity

10.1007/s007050050355

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Citations (9)

Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan

Infection Genetics and Evolution (2017)

Moegi Kuroda Tsuneyuki Masuda Mika Ito Yuki Naoi Yen Hai Doan

Sapovirus

Strain (injury)

10.1016/j.meegid.2017.09.013

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Citations (26)

Morphologic study of inclusions in tissues from pigs inoculated with cytomegalovirus

American Journal of Veterinary Research (1987)

Minoru Narita H. Kawamura Junsuke Shirai Makoto HARITANI

SUMMARY Six 5-day-old gnotobiotic pigs inoculated intranasally with the J-1 strain of porcine cytomegalovirus ( pcmv ) were euthanatized within 9 to 18 days after inoculation. All pigs developed a subclinical form of pcmv infection and had characteristic intranuclear inclusions in all tissues. Morphologically, 2 types of inclusions were observed. One was a small herpetic intranuclear inclusion in reticuloendothelial cells, which contained a few nucleocapsids in the nucleus. The other was a typical cytomegalic intranuclear inclusion in macrophages in the lungs and in tubuloalveolar gland cells in the nasal mucosa. Inclusions contained many nucleocapsids in the nucleus and a large number of enveloped viral particles in the cytoplasm. The distribution of the specific immunofluorescent antigen of pcmv was coincident with the presence of herpetic and cytomegalic inclusions.

Cytomegalovirus

10.2460/ajvr.1987.48.09.1398

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Citations (7)

Molecular, biological, and antigenic characterization of a Border disease virus isolated from a pig during classical swine fever surveillance in Japan

Journal of Veterinary Diagnostic Investigation (2014)

Makoto Nagai Hiroshi Aoki Yoshihiro Sakoda Takashi Kozasa Kaho Tominaga-Teshima

In the current study, molecular, biological, and antigenic analyses were performed to characterize Border disease virus (BDV) strain FNK2012-1 isolated from a pig in 2012 in Japan. The complete genome comprises 12,327 nucleotides (nt), including a large open reading frame of 11,685 nt. Phylogenetic analysis revealed that FNK2012-1 was clustered into BDV genotype 1 with ovine strains. FNK2012-1 grew in porcine, bovine, and ovine primary cells and cell lines, but grew better in bovine and ovine cells than in porcine cells. Specific pathogen-free pigs inoculated with FNK2012-1 did not show any clinical signs. Noninoculated contact control pigs also did not show clinical signs and did not seroconvert. The results suggest that FNK2012-1 may be of ruminant origin and is poorly adapted to pigs. Such observations can provide important insights into evidence for infection and transmission of BDV, which may be of ruminant origin, among pigs.

Domestic pig

Classical swine fever

10.1177/1040638714541837

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Citations (17)

Anti-Viral Effect Evidenced from Carbon Silk Made from Natural Silk

Journal of the Japan Veterinary Medical Association (2008)

Junsuke Shirai Masamitu MIYASHITA Mariko SAKAGAMI Kenichi Nakajima Chiyuki Takabayashi

Carbon Silk (CS) is made from silk fabrics heated to around 1000ºC, and has been shown to have an anti-bacterial effect. We therefore examined the ability of CS to eliminate viruses, with three envelope viruses (transmissible gastroenteritis virus, Audieskv's virus, vesicular stomatitis virus) and two non-envelope viruses (footand mouth disease virus (FMDV), swine vesicular disease virus). CS showed a significant ability to eliminate viruses against three envelope viruses and FMDV. This effect was enhanced by increasing the quantities of CS, but was not affected by reaction time or temperature, and it was observed in repetitive reactions. The mechanism of this CS effect was considered to consist of electrostatic interactions between virus particles and the N radical in the CS.

Vesicular Stomatitis

10.12935/jvma1951.61.48

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Citations (1)

Outbreak of Comb Necrosis in Layer Breeder Chickens

Avian Diseases (1997)

Kikuyasu Nakamura Junsuke Shirai Kunitoshi Imai H Hihara Nobuhiko TANIMURA

Comb necrosis with leg weakness was seen in 41-day-old female layer breeder chickens. This disease occurred in three flocks at a breeder farm, but not in other flocks of growing chickens and broiler breeder hens at the same farm. The disease started in 35-day-old chicks in three flocks. The morbidity of comb necrosis was 10% and that of leg weakness was 3%. Characteristic gross lesions of affected chickens were swelling and necrosis of the whole comb. Histologically, liquefactive necrosis of epidermal epithelial cells with hyperplasia, vesicle formation in the epidermis, congestion, and hemorrhages with fibrinous thrombi of underlying dermis in the comb were noted. In mature comb lesions, the epidermis showed eosinophilic necrosis (scab formation). In the livers, multiple fibrinous thrombi were present in the sinusoids and there was necrosis of hepatic cells. Staphylococcus aureus and Pasturella spp. were isolated from comb lesions. There were no significant lesions causing leg weakness.

Flock

Epidermis (zoology)

10.2307/1592467

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Development of Reverse Transcriptase PCR and Nested PCR to Detect Porcine Hemagglutinating Encephalomyelitis Virus

Journal of Veterinary Medical Science (2004)

Yoshiko Sekiguchi Junsuke Shirai Takahide Taniguchi Eiichi HONDA

Porcine hemagglutinating encephalomyelitis virus (HEV) causes encephalomyelitis, or vomiting and wasting disease, in suckling piglets. The mortality rate for piglets under 3 weeks old is 100%, but they are usually protected by maternal antibodies. Recently, the risk of an HEV outbreak has increased in the pig industry, because of widely using specific pathogen-free pigs that have no antibodies to HEV. We developed reverse transcription (RT) PCR and nested PCR to detect HEV. Primer sets of polymerase, non-structural protein, and spike protein were designed for RT-PCR and nested PCR based on the nucleotide sequences of the HEV 67N strain. The PCR designated primer sets of spike protein detected only HEV viral RNA among other related nidoviruses. Detection of HEV viral RNA by nested PCR was more sensitive than virus isolation in cell cultures. Nested PCR detected HEV viral RNA from experimentally infected samples of mice and field samples of piglets. The RT-PCR and nested PCR methods to detect HEV is considered a good way to show the HEV etiology on pig farms.

Primer (cosmetics)

10.1292/jvms.66.367

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Citations (11)

Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan

Archives of Virology (2016)

Yuki Naoi Mai Kishimoto Tsuneyuki Masuda Mika Ito Shinobu Tsuchiaka

Picornavirus

Medical microbiology

Picornaviridae

10.1007/s00705-016-2834-7

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Citations (9)

Gene design of signal sequence for the effective secretion of recombinant protein using insect cell

Nucleic Acids Symposium Series (2005)

Yoshinori Tsuchiya Kazuki Morioka Isao Taneda Junsuke Shirai Kazuo Yoshida

In order to increase the proteinic secretion quantity of production in insect cell, the chicken lysozyme signal peptide (CLSP) was altered, and the effect of mutations on secretion of human lysozyme (HLY), the bovine interferon beta-3, and the equine interferon alpha-1 were studied. A hybrid gene composed of CLSP gene and HLY gene was used for the production of recombinant HLY in insect cell (BmN4) using vacuole virus expression system. In contrast with the Leu rich signal peptide in previous reports, Val rich type, or Ile rich type decreased the secretion of HLY, and Phe rich type prevented it completely, in spite of the increase of hydrophobicity in the central core region of each signal peptide. Besides the hydrophobicity, the tertiary structure in this region also might influence the secretion of HLY in insect cells. Then, Leu-rich signal peptide was used for the secretion of the bovine interferon beta-3, or the equine interferon alpha-1, resulted in the remarkable increase of the amount of the secretion in insect cells.

10.1093/nass/49.1.305

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Citations (7)

Newcastle disease viruses isolated from racing pigeons in Japan.

The Japanese Journal of Veterinary Science (1986)

Junsuke Shirai Kenji Tsukamoto H Hihara

During the period from September 1983 to October 1984, outbreaks of Newcastle disease (ND) in racing pigeons occurred in Japan. Seven isolates were collected from these outbreaks in 7 prefectures. All of the isolates were found to be identical with ND virus by the hemagglutination inhibition test, and they formed plaques without addition of diethyl-aminoethyl-dextran and MgS04 in tissue culture systems. Mean death time of the minimum lethal dose (MDT/MLD) of 7 isolates ranged from 90 to 144 hrs. The intracerebral pathogenicity indices of 5 isolates ranged from 1.3 to 1.7. Five out of 7 isolates were inoculated orally into 1-week-old or 4-week-old chickens. All of the isolates induced clinical signs in 1-week-old chicks, and 3 out of 5 isolates induced death in a few chicks (mortality, 10-30%). However, none of the 5 isolates induced clinical signs in most of the 4-week-old chickens. These results indicated that all the present isolates were of a mesogenic strain while showing a comparatively longer MDT/MLD.

Newcastle Disease

Brain tissue

10.1292/jvms1939.48.1091

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Citations (10)