Roberto Mantovani

University of Milan

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Diletta Dolfini

University of Milan

Nerina Gnesutta

University of Milan

Carol Imbriano

University of Modena and Reggio Emilia

Maurizio D’Incalci

Humanitas University

Cristiana Forni

Istituto Ortopedico Rizzoli

Sergio Ottolenghi

University of Milano-Bicocca

Mario Minuzzo

Sofar (Italy)

Eugenio Erba

Mario Negri Institute for Pharmacological Research

M Viganò

University of Basel

Raffaella Gatta

King University

Cooperative Institutions

University of Milan

185

Istituti di Ricovero e Cura a Carattere Scientifico

130

Centre National de la Recherche Scientifique

117

Inserm

110

University of Turin

Weatherford College

University of Bologna

Harvard University

Istituto Nazionale di Fisica Nucleare

Mario Negri Institute for Pharmacological Research

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The Plant NF-Y DNA Matrix In Vitro and In Vivo

Plants (2019)

Nerina Gnesutta Matteo Chiara Andrea Bernardini Matteo Balestra David S. Horner

Nuclear Factor Y (NF-Y) is an evolutionarily conserved trimer formed by a Histone-Fold Domain (HFD) heterodimeric module shared by core histones, and the sequence-specific NF-YA subunit. In plants, the genes encoding each of the three subunits have expanded in number, giving rise to hundreds of potential trimers. While in mammals NF-Y binds a well-characterized motif, with a defined matrix centered on the CCAAT box, the specificity of the plant trimers has yet to be determined. Here we report that Arabidopsis thaliana NF-Y trimeric complexes, containing two different NF-YA subunits, bind DNA in vitro with similar affinities. We assayed precisely sequence-specificity by saturation mutagenesis, and analyzed genomic DNA sites bound in vivo by selected HFDs. The plant NF-Y CCAAT matrix is different in nucleotides flanking CCAAT with respect to the mammalian matrix, in vitro and in vivo. Our data point to flexible DNA-binding rules by plant NF-Ys, serving the scope of adapting to a diverse audience of genomic motifs.

CAAT box

Sequence motif

genomic DNA

Trimer

10.3390/plants8100406

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Citations (10)

Direct non transcriptional role of the CCAAT-factor NF-Y in DNA replication

Silvia Belluti Paolo Benatti Benoît Miotto Julia Neusiedler Diletta Dolfini

Replication

Replication factor C

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Data from Trabectedin (ET-743) promotes differentiation in myxoid liposarcoma tumors

Cristiana Forni Mario Minuzzo Emanuela Virdis Elena Tamborini Matteo De Simone

<div>AbstractDifferentiation is a complex set of events that can be blocked by rearrangements of regulatory genes producing fusion proteins with altered properties. In the case of myxoid liposarcoma (MLS) tumors, the causative abnormality is a fusion between the CHOP transcription factor and the FUS or EWS genes. CHOP belongs to and is a negative regulator of the large CAAT/enhancer binding protein family whose α, β,and δ members are master genes of adipogenesis. Recent clinical data indicate a peculiar sensitivity of these tumors to the natural marine compound trabectedin. One hypothesis is that the activity of trabectedin is related to the inactivation of the FUS-CHOP oncogene. We find that trabectedin causes detachment of the FUS-CHOP chimera from targeted promoters. Reverse transcription-PCR and chromatin immunoprecipitation analysis in a MLS line and surgical specimens of MLS patients in vivo show activation of the CAAT/enhancer binding protein–mediated transcriptional program that leads to morphologic changes of terminal adipogenesis. The activity is observed in cells with type 1 but not type 8 fusions. Hence, the drug induces maturation of MLS lipoblasts in vivo by targeting the FUS-CHOP–mediated transcriptional block. These data provide a rationale for the specific activity of trabectedin and open the perspective of combinatorial treatments with drugs acting on lipogenic pathways. [Mol Cancer Ther 2009;8(2):449–57]</div>

Trabectedin

Myxoid liposarcoma

Chromatin immunoprecipitation

10.1158/1535-7163.c.6532110.v1

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Author Index

Journal of Biological Chemistry (1998)

A. I. Akhmedov Albrechtsen Reidar Emad S. Alnemri Hadi Alzuherri Anazawa Hideharu

10.1016/s0021-9258(18)80584-1

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Sardinian G gamma-HPFH: a T----C substitution in a conserved "octamer" sequence in the G gamma-globin promoter

Blood (1988)

Sergio Ottolenghi Silvia K. Nicolis Roberto Taramelli Nicoletta Malgaretti Roberto Mantovani

10.1182/blood.v71.3.815.815

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Citations (59)

NF-Y splice variants differentially affect skeletal myogenesis

Valentina Basile Fiorenza Baruffaldi Diletta Dolfini Laura Ricci Roberto Mantovani

splice

Affect

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Expression of the CCAAT-binding factor NF-Y in Caenorhabditis elegans

Journal of Molecular Histology (2005)

Antonella Franchini Carol Imbriano Elisa Peruzzi Roberto Mantovani Enzo Ottaviani

10.1007/s10735-004-6017-6

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Citations (9)

Pin1 Links the Activities of c-Abl and p300 in Regulating p73 Function

Molecular Cell (2004)

Fiamma Mantovani Silvano Piazza Monica Gostissa Sabrina Strano Paola Zacchi

10.1016/j.molcel.2004.05.007

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Citations (181)

Sequence-Specific Transcription Factor NF-Y Displays Histone-like DNA Binding and H2B-like Ubiquitination

Cell (2013)

Marco Nardini Nerina Gnesutta Giacomo Donati Raffaella Gatta Cristiana Forni

CAAT box

Histone H2B

Transcription

HMG-box

DNA binding site

10.1016/j.cell.2012.11.047

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Citations (279)

CCAAT-box binding protein NF-Y (CBF, CP1)recognizes the minor groove and distorts DNA

Nucleic Acids Research (1995)

Antonella Ronchi Marianna Bellorini Nicola Mongelli Roberto Mantovani

Journal Article CCAAT-box binding protein NF-Y (CBF, CP1)recognizes the minor groove and distorts DNA Get access Antonella Ronchi, Antonella Ronchi Search for other works by this author on: Oxford Academic PubMed Google Scholar Marianna Bellorini, Marianna Bellorini Search for other works by this author on: Oxford Academic PubMed Google Scholar Nicola Mongelli, Nicola Mongelli 1Pharmacia/Farmitalia Carlo Erba, Nerviano Research CenterVia Papa Giovanni XXIII 68, 20014 Nerviano, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar Roberto Mantovani Roberto Mantovani * *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 23, Issue 22, 25 November 1995, Pages 4565–4572, https://doi.org/10.1093/nar/23.22.4565 Published: 01 January 1995 Article history Published: 01 January 1995 Received: 21 September 1995 Accepted: 11 October 1995

Groove (engineering)

Minor (academic)

10.1093/nar/23.22.4565

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Citations (86)