Susan K. Dutcher

Washington University in St. Louis

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i-10 index

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Document Type

Co-Authors

Stephen S. Rich

University of Virginia

Eric Boerwinkle

The University of Texas Health Science Center at Houston

Joanne E. Curran

The University of Texas Rio Grande Valley

Rasika A. Mathias

Johns Hopkins Medicine

Søren Germer

New York Genome Center

Ruth J. F. Loos

Icahn School of Medicine at Mount Sinai

Donna K. Arnett

University of South Carolina

Myriam Fornage

Brown Foundation

John Blangero

The University of Texas Rio Grande Valley

Lisa R. Yanek

Johns Hopkins Medicine

Cooperative Institutions

Harvard University

419

Brigham and Women's Hospital

273

University of Washington

272

Washington University in St. Louis

255

Broad Institute

238

Beth Israel Deaconess Medical Center

214

Massachusetts General Hospital

211

University of Helsinki

197

National Institutes of Health

182

Institute for Molecular Medicine Finland

180

Author Statistics

Papers

Citation

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Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies.

Genetics (1995)

Linda L. Ehler Jeffrey Holmes Susan K. Dutcher

The bld2-1 mutation in the green alga Chlamydomonas reinhardtii is the only known mutation that results in the loss of centrioles/basal bodies and the loss of coordination between spindle position and cleavage furrow position during cell division. Based on several different assays, bld2-1 cells lack basal bodies in > 99% of cells. The stereotypical cytoskeletal morphology and precise positioning of the cleavage furrow observed in wild-type cells is disrupted in bld2-1 cells. The positions of the mitotic spindle and of the cleavage furrow are not correlated with respect to each other or with a specific cellular landmark during cell division in bld2-1 cells. Actin has a variable distribution during mitosis in bld2-1 cells, but this aberrant distribution is not correlated with the spindle positioning defect. In both wild-type and bld2-1 cells, the position of the cleavage furrow is coincident with a specialized set of microtubules found in green algae known as the rootlet microtubules. We propose that the rootlet microtubules perform the functions of astral microtubules and that functional centrioles are necessary for the organization of the cytoskeletal superstructure critical for correct spindle and cleavage furrow placement in Chlamydomonas.

Chlamydomonas reinhardtii

Chlamydomonas

Strain (injury)

Basal (medicine)

Basal body

10.1093/genetics/141.3.945

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Citations (113)

Real-Time Subcellular Localization Reveals Hidden Intraflagellar Transport Mechanisms

Biophysical Journal (2017)

Anthony Kovacs Jonathan Kessler Je-Luen Li Huawen Lin Susan K. Dutcher

Colocalization

Organelle

Intraflagellar Transport

Kinesin

10.1016/j.bpj.2016.11.1695

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Citations (0)

Basal Feet: Walking to the Discovery of a Novel Hybrid Cilium

Developmental Cell (2020)

Rachael M. Fewell Susan K. Dutcher

Basal body

Basal (medicine)

Motile cilium

Polarity (international relations)

Cell polarity

10.1016/j.devcel.2020.09.018

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Citations (3)

Genome sequencing unveils a regulatory landscape of platelet reactivity

Nature Communications (2021)

Ali R. Keramati Ming‐Huei Chen Benjamin A.T. Rodriguez Lisa R. Yanek Arunoday Bhan

Abstract Platelet aggregation at the site of atherosclerotic vascular injury is the underlying pathophysiology of myocardial infarction and stroke. To build upon prior GWAS, here we report on 16 loci identified through a whole genome sequencing (WGS) approach in 3,855 NHLBI Trans-Omics for Precision Medicine (TOPMed) participants deeply phenotyped for platelet aggregation. We identify the RGS18 locus, which encodes a myeloerythroid lineage-specific regulator of G-protein signaling that co-localizes with expression quantitative trait loci (eQTL) signatures for RGS18 expression in platelets. Gene-based approaches implicate the SVEP1 gene, a known contributor of coronary artery disease risk. Sentinel variants at RGS18 and PEAR1 are associated with thrombosis risk and increased gastrointestinal bleeding risk, respectively. Our WGS findings add to previously identified GWAS loci, provide insights regarding the mechanism(s) by which genetics may influence cardiovascular disease risk, and underscore the importance of rare variant and regulatory approaches to identifying loci contributing to complex phenotypes.

Genome-wide Association Study

10.1038/s41467-021-23470-9

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Citations (38)

Asymmetries in the cilia ofChlamydomonas

Philosophical Transactions of the Royal Society B Biological Sciences (2019)

Susan K. Dutcher

The generation of ciliary waveforms requires the spatial and temporal regulation of dyneins. This review catalogues many of the asymmetric structures and proteins in the cilia of Chlamydomonas , a unicellular alga with two cilia that are used for motility in liquid medium. These asymmetries, which have been identified through mutant analysis, cryo-EM tomography and proteomics, provide a wealth of information to use for modelling how waveforms are generated and propagated. This article is part of the Theo Murphy meeting issue ‘Unity and diversity of cilia in locomotion and transport’.

Chlamydomonas

Motile cilium

10.1098/rstb.2019.0153

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Citations (31)

Analysis of Chlamydomonas reinhardtii Genome Structure Using Large‐Scale Sequencing of Regions on Linkage Groups I and III

Journal of Eukaryotic Microbiology (2003)

Jin Billy Li Shaoping Lin Honggui Jia Hongmin Wu Bruce A. Roe

Chlamydomonas reinhardtii is a unicellular green alga that has been used as a model organism for the study of flagella and basal bodies as well as photosynthesis. This report analyzes finished genomic DNA sequence for 0.5% of the nuclear genome. We have used three gene prediction programs as well as EST and protein homology data to estimate the total number of genes in Chlamydomonas to be between 12,000 and 16,400. Chlamydomonas appears to have many more genes than any other unicellular organism sequenced to date. Twenty-seven percent of the predicted genes have significant identity to both ESTs and to known proteins in other organisms, 32% of the predicted genes have significant identity to ESTs alone, and 14% have significant similarity to known proteins in other organisms. For gene prediction in Chlamydomonas, GreenGenie appeared to have the highest sensitivity and specificity at the exon level, scoring 71% and 82%. respectively. Two new alternative splicing events were predicted by aligning Chlamydomonas ESTs to the genomic sequence. Finally recombination differs between the two sequenced contigs. The 350-Kb of the Linkage group III contig is devoid of recombination, while the Linkage group I contig is 30 map units long over 33-kb.

Chlamydomonas

Chlamydomonas reinhardtii

Synteny

Nuclear gene

Homology

Comparative Genomics

10.1111/j.1550-7408.2003.tb00109.x

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Citations (27)

Publisher Correction: Deep coverage whole genome sequences and plasma lipoprotein(a) in individuals of European and African ancestries

Nature Communications (2020)

Seyedeh M. Zekavat Sanni Ruotsalainen Robert E. Handsaker Maris Alver Jonathan M. Bloom

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

10.1038/s41467-020-15236-6

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Citations (2)

Elucidation of Basal Body and Centriole Functions in Chlamydomonas reinhardtii

Traffic (2003)

Susan K. Dutcher

In eukaryotic cells, basal bodies and their structural equivalents, centrioles, play essential roles. They are needed for the assembly of flagella or cilia as well as for cell division. Chlamydomonas reinhardtii provides an excellent model organism for the study of the basal body and centrioles. Genes for two new members of the tubulin superfamily are needed for basal body/centriole duplication. In addition, other genes that play roles in the duplication and segregation of basal bodies are discussed.

Basal body

Centriole

Chlamydomonas reinhardtii

Chlamydomonas

Basal (medicine)

Motile cilium

10.1034/j.1600-0854.2003.00104.x

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Citations (111)

Rare variants in long non-coding RNAs are associated with blood lipid levels in the TOPMed whole-genome sequencing study

The American Journal of Human Genetics (2023)

Yuxuan Wang Margaret Sunitha Selvaraj Xihao Li Zilin Li Jacob A. Holdcraft

Genome-wide Association Study

Genetic Association

Genetic architecture

10.1016/j.ajhg.2023.09.003

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Citations (7)

Mutations in α-tubulin promote basal body maturation and flagellar assembly in the absence of δ-tubulin

Journal of Cell Science (2003)

Sylvia Fromherz Thomas H. Giddings Natalia Gomez‐Ospina Susan K. Dutcher

We have isolated suppressors of the deletion allele of delta-tubulin, uni3-1, in the biflagellate green alga Chlamydomonas reinhardtii. The deletion of delta-tubulin produces cells that assemble zero, one or two flagella and have basal bodies composed primarily of doublet rather than triplet microtubules. Flagellar number is completely restored in the suppressed strains. Most of the uni3-1 suppressors map to the TUA2 locus, which encodes alpha2-tubulin. Twelve independent tua2 mutations were sequenced. Amino acids D205 or A208, which are nearly invariant residues in alpha-tubulin, were altered. The tua2 mutations on their own have a second phenotype - they make the cells colchicine supersensitive. Colchicine supersensitivity itself is not needed for suppression and colchicine cannot phenocopy the suppression. The suppressors partially restore the assembly of triplet microtubules. These results suggest that the delta-tubulin plays two roles: it is needed for extension or stability of the triplet microtubule and also for early maturation of basal bodies. We suggest that the mutant alpha-tubulin promotes the early maturation of the basal body in the absence of delta-tubulin, perhaps through interactions with other partners, and this allows assembly of the flagella.

Basal body

Phenocopy

Chlamydomonas

Chlamydomonas reinhardtii

Macronucleus

Colchicine

10.1242/jcs.00859

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Citations (24)