Sri Hartati

Sebelas Maret University

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Ongko Cahyono

Sebelas Maret University

Slamet Minardi

Sebelas Maret University

Enny Sudarmonowati

Agence Nationale de la Recherche

Catur Budi Handayani

Universitas Bangun Nusantara

Rika Meliansyah

Padjadjaran University

Supriyadi Supriyadi

Sebelas Maret University

Jauhari Syamsiyah

Sebelas Maret University

Agustina Intan Niken Tari

Universitas Bangun Nusantara

Novian Wely Asmoro

Universitas Bangun Nusantara

Hery Widijanto

Sebelas Maret University

Cooperative Institutions

Sebelas Maret University

127

National Research and Innovation Agency

Indonesian Institute of Sciences

Padjadjaran University

Universitas Gadjah Mada

IPB University

University of Indonesia

Indonesian Agency for Agricultural Research and Development

Pusat Penelitian dan Pengembangan Peternakan

Universitas Surakarta

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The Abilities of Bacteria and Yeast Isolated from Vermicompost Water Extract to Inhibit Alternaria solani in vitro and Early Blight Disease on Tomato

Cropsaver Journal of Plant Protection (2022)

Noor Istifadah Retno Anjani Putri Sri Hartati

Early blight disease (Alternaria solani Sor) is one of limiting factors in tomato production. Bacteria and yeast are potential for biocontrol of plant diseases. Sources to obtain biocontrol agens is water extract of organic matters. The objective of this study was to examine the abilities of bacteria and yeast isolated from vermicompost water extract in inhibiting the growth of A. solani in vitro and suppressing the pathogen infection in tomato fruits and leaves. Two types of vermicompost used in this study were cattle manure vermicompost and goat manure vermicompost. The isolation of bacteria and yeast from the vermicompost water extract resulted in 14 isolates, which were 10 isolates from cattle manure vermicompost and four isolates from goat manure vermicompost. Among the isolates, six isolates (three yeast and three bacterial isolates) inhibited the growth of A. solani in vitro by 42.8% – 79.1%. In tomato fruits, five isolates inhibited A. solani infection by 70.6% - 100.0%. In tomato plants, four isolates suppressed early blight disease in tomato leaves by 56.2% - 83.5%. The isolate that showed consistent effects in vitro as well as in vivo was bacterial isolate KB 3. This isolate was potential as biocontrol agent of tomato diseases caused by A. solani.

Alternaria solani

10.24198/cropsaver.v4i2.37374

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AKTIVITAS ANTIOKSIDAN DAUN DAN UMBI DARI ENAM JENIS SINGKONG (Manihot utilissima Pohl) - (Antioxidant Activity of Leaves and Tuber from Six Types of Cassava (Manihot utilissima Pohl)

DOAJ (DOAJ: Directory of Open Access Journals) (2016)

Fauzy Rachman Sri Hartati Enny Sudarmonowati Partomuan Simanjuntak

Cassava is a staple food after rice and maize in Indonesia. In general, parts of cassava plants that consumed are the leaves and tubers. This research aimed to compare antioxidant activity of leaves and tuber from six types of cassava ( Manihot utilissima Pohl). The leaves and tubers of the cassava plant from Cibinong were extracted with methanol separately. Methanol extract was measured the inhibition percentage as an antioxidant using DPPH reagent at 100 ppm. Results showed that Pucuk Biru leaves type has the highest inhibition at 88.09% and IC 50 of 45.161 ppm. Keywords : antioxidant, Cibinong, DPPH, IC 50 , Manihot utilissima Pohl ABSTRAK Ketela pohon merupakan bahan pangan pokok setelah beras dan jagung di Indonesia. Bagian tanaman singkong yang umumnya dikonsumsi adalah daun dan umbi. Penelitian ini bertujuan untuk membandingkan aktivitas antioksidan daun dan umbi dari enam jenis singkong ( Manihot utilissima Pohl). Daun dan umbi tanaman singkong dari Cibinong masing-masing diekstraksi secara terpisah menggunakan pelarut metanol. Ekstrak metanol yang diperoleh diukur persentase daya inhibisinya menggunakan metode peredaman radikal bebas dengan reagen DPPH pada konsentrasi 100 bpj. Hasil pengujian antioksidan menunjukkan bahwa bagian daun singkong jenis Pucuk Biru mempunyai daya inhibisi yang paling tinggi yaitu 88,09% dan IC 50 sebesar 45,16 bpj. Kata kunci : antioksidan, Cibinong, DPPH, IC 50 , singkong

Manihot

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Optimization of cellulose nanofiber production from oil palm empty fruit bunch using Trichoderma sp. with the solid state fermentation method

BioResources (2019)

Pingkan Aditiawati Rudi Dungani Redian Mayo Fikri Sri Hartati

Cellulose nanofibers were isolated from oil palm empty fruit bunch by delignification using Marasmius sp. and subsequently treated by solid state fermentation using Trichoderma sp. This method used pH values of 4.8, 5.5, and 7 and temperatures of 28 °C, 32 °C, and 37 °C for 7 d. Scanning electron microscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and particle size analysis were performed to determine the properties of the isolated cellulose nanofibers. Results showed that chemical analysis by FTIR, lignin was completely removed from the EFB. It was found that the combination of pH 5.5 and incubation temperature 37 °C were favorable for the SSF process to isolate cellulose nanofibers from oil palm EFB fiber. SSF processing resulted in smooth morphological structures, with a fiber diameter range of 32.6 nm to 36.4 nm.

Solid-State Fermentation

10.15376/biores.14.2.3688-3700

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Soil Bioremediation of lead (Pb) polluted paddy field using Mendong (Fimbristylis globulosa), Rhizobium SpI₃, compost, and inorganic fertilizer

IOP Conference Series Earth and Environmental Science (2019)

Retno Rosariastuti D P Mahardika Supriyadi Supriyadi Purwanto Purwanto Sri Hartati

Industrial developments cause an increase in lead (Pb) pollution in the paddy field because irrigation water is mixed with industrial liquid waste. This problem needs to be handled. Without proper treatment, lead can enter the human body either directly or indirectly through the food chain cycle, thus endangering human health. One method that can be used is bioremediation; a technology to restore the environment (paddy field) polluted by heavy metals using biological agents. The purpose of this research is to analyze the ability of Mendong plants (Fimbristyli globulosa) combined with inorganic fertilizers and chelators (Rhizobium spI3 and compost) in absorbing lead in the paddy field. The research was conducted in Jaten Sub-district, Karanganyar Regency in April-October 2016. Randomized block design experimental method completed with the factorial pattern was applied as the research method. There were three treatment factors, namely inorganic fertilizer, chelator, and plant with three replications. The results show that some soil characteristics such as soil pH, cation exchange capacity (CEC), Organic Material (OM), and total microbes impact the availability of lead in the soil to be absorbed by plants. The highest uptake of lead occurred in the treatment of inorganic fertilizer + bacteria + Mendong plants (P1B1T1) amounting to 52.01 mg/kg at the root and 38.6 mg/kg in the shoot section. The Mendong plants combined with Rhizobium spI3 has the potential to be an alternative bioremediation technology on lead-polluted paddy field soils.

Paddy field

10.1088/1755-1315/230/1/012014

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Dinamika Populasi Bakteri Heterotrof dan Nitrifikasi pada Sistem Agroforestri di Sub-DAS Keduang, Daerah Bengawan Solo Hulu The Dynamics Population of The Heterotrophic and Nitrifying Bacteria on Agroforestry System in Keduang Sub Watershed in Bengawan Solo Hulu Region

Sri Hartati

Nitrogen is the main problem in soil, one of them is nitrate compound is water soluble and easy to be leached in the soil water and become pollution substances in the soil and water. The research aim is to study about the potential of trees litter in agroforestry system were estimated have the natural nitrification inhibitory effect and heterotrophic microbe in the soil. This research was conducted by survey and usingdestructivesoil sampling. The nitrification potential in the soil was measured by Berg and RosswallMethod modification from Kandeler, population and activity of the nitrifying microbe using MPN method, and population of the heterotrophic microbe by using Plate Count method.Result of the research are : the all treeslitter observed in the study candecline the nitrification rate, potential nitrification value and increasing the amount of Heterotrophic Bacteriasignificantly. The using the litter of plants can be recommended as an alternative way in controlling nitrification process naturally, environmental safety and supporting continuous agriculture system.

Nitrifying Bacteria

Litter

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Antioxidant activities of phenolic compounds isolated from the leaves of Macaranga allorobinsonii Whitmore

AIP conference proceedings (2017)

Akhmad Darmawan Sofa Fajriah Megawati Megawati Indah Dwiatmi Dewijanti Sofna Banjarnahor

Two secondary metabolites compounds, gallic acid (1) and methyl gallate (2) have been isolated from the ethyl acetate fraction of the methanol extract of the leaves of Macaranga allorobinsonii Whitmore. Isolation and purification of the secondary metabolite compounds conducted using chromatography methods, and structure elucidation determined based on NMR, mass spectroscopic data and compared with appropriate references.

Methyl gallate

Fraction (chemistry)

Secondary metabolite

Chemical structure

10.1063/1.4973147

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Pertumbuhan dan Hasil Kacang Hijau (Vigna radiata, L.) pada Beberapa Dosis Pupuk Organik dan Kerapatan Tanam

Caraka Tani Journal of Sustainable Agriculture (2018)

Desi Putri Hastuti Supriyono Supriyono Sri Hartati

Mungbean is one of the strategic annual food crops that needed by Indonesian people because of the high protein and minerals. Production of mungbean in Indonesia always decreases. Cultural improvements are needed to increase productivity i.e by appropriate fertilization and plant density. The experiment objective was to find out the optimum dosage of organic fertilizer and plant density for the growth and yield of mungbean. The experiment was carried out using factorial completely randomized design (CRD). The first factor treatments were organic fertilizer which consisted of 0, 5, 10, 15, and 20 ton ha-1, while the second-factor treatments were planted density which consisted of 1 and 2 plants in the hole. Organic fertilizer of 5 ton ha-1 gave the best result for plant height, number of branches, flowering age, number of pods, fresh weight and dried straw, weight of 100 seeds, number and weight of seeds, small of seeds number and P nutrient uptake of the plant. The 2 plants per hole gave the best result for branches number, pods number and number of small seeds. Application of 5 ton ha-1 organic fertilizer and 1 plant per hole gave better yield. No interaction of organic fertilizer and plant density to mungbean growth and yield.

Organic fertilizer

10.20961/carakatani.v33i2.20412

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Citations (25)

Diversity induction with gamma ray irradiation on Dendrobium odoardi orchid

IOP Conference Series Earth and Environmental Science (2021)

Tilmiidzah Salma Fathin Sri Hartati Ahmad Yunus

Abstract Orchid is an ornamental plant commodity that has potential to be developed. One species of orchid that is in demand is Dendrobium odoardi . The aims of this research is to determine the characteristics and morphological diversity of Dendrobium odoardi orchid irradiated gamma ray results and without gamma ray irradiation. This research was conducted in two places, the implementation of gamma ray irradiation at the PATIR-BATAN Laboratory and subculture in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, UNS. The research was conducted start from March 2019 – January 2020. The research used a simple design of one factor, the doses of gamma ray irradiation with 5 levels, 0 Gy (control), 10 Gy, 15 Gy, 20 Gy, and 25 Gy. Each level of treatment was planted and observed 50 plantlets. Observational data were analyzed descriptively by comparing observational data from Dendrobium odoardi orchids irradiated with control plants. The results showed that the induction of gamma ray irradiation resulted changes in morphological characters in Dendrobium odoardi orchid such as, increasing plant height, increasing leaf width, decreasing number of roots, decreasing root length, change in leaf shape and change in leaf color. Changes in the morphological character of Dendrobium odoardi orchids produce plant diversity.

Dendrobium

Ornamental plant

Subculture (biology)

Cut flowers

Gamma Irradiation

10.1088/1755-1315/637/1/012035

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Citations (7)

The Survival Rate and Starch Histochemical Assay of Various Stem Cutting Conditions of Mentega 2 Cassava Genotype at Initial Growth Stage

BIOEDUSCIENCE (2021)

Sri Hartati Hani Fitriani Nanang Taryana Nawawi Nawawi Yani Cahyani

Background: This study aimed to evaluate the growth ability of cassava mini stem cuttings with different node number and a variety of stem cutting shapes and their correlation with starch content in the stems at initial growth stages. Methods. In this study, the viability of cassava stem cuttings was identified in two type experiments i.e. mini-stem cuttings consisting 1 and 2 nodes and shape variation of single node mini-stem cutting. Parameters observed were shoots emergence period, number of sprouting cuttings, shoots number of individual stem cuttings, shoots height and number of leaves. In addition, starch histochemical test was also carried out on stems of young shoots and initial stem cuttings using Lugol’s solution. Results. Both cassava stem cuttings consisting of 1 and 2 buds indicated the same survival rate of 100%. 1 bud stem cuttings with different shapes showed different survival rate, i.e. 60-80% for semicircular and fully circular cuttings and 30-40% for box shape cuttings. The difference in survival rate with different stem size is probably related to the availability of the amount of starch to support shoots growth. Observations at week 3 after planting generally showed that the stem cuttings with 2 buds were higher than those of stem with 1 bud. Conclusion: There were differences in the scores on the starch content test qualitatively with Lugol staining, in various parts of the plant originating from 1 bud and 2 bud cuttings which may indicate a breakdown of starch during shoot development.

Cutting

Sprouting

10.22236/j.bes/515612

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Citations (3)

Nilai LD50 dan LC50 Ekstrak Etanol Herba Ketumpangan Air (Peperomia pellucida (L.) Kunth)

JURNAL ILMU KEFARMASIAN INDONESIA (2017)

Indah Dwiatmi Dewijanti Marissa Angelina Sri Hartati Betty Ernawati Dewi Lia Meilawati

Ketumpangan air (Peperomia pellucida (L.) Kunth), an Indonesian wild plant, is commonly found in every Indonesian region with moist shady place. People usually consume ketumpangan air as a fresh vegetables. Empirically, ketumpangan air is consumed to treat fever, stomach disease, kidney disorders, drug abscesses, boils acne, headaches, pain in arthritic and rheumatic gout, or other external treatment. In this research, we tested the acute toxicity of ethanol extract from ketumpangan air using experimental animals mice DDY strain. We also tested the Brine Shrimp Lethality Test (BSLT) using Artemia salina Leach larva shrimp. The main aim of this reseach was to determine the LD50 and LC50 values of ethanol extract from Ketumpangan air. The results showed that the ethanol extract was categorized as ‘mild toxic’. The calculation of LD50 with Reed and Munich method showed that the LD50 value for male and female group was 15.13 g/ kg and 11.87 g/kg respectively. The BSLT result showed that the LC50 values of Ketumpangan air was 81.28 ppm.

Brine shrimp

Artemia salina

Median lethal dose

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Citations (2)