E Papi

Sapienza University of Rome

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G. Petrone

Sapienza University of Rome

Piera Valenti

Sapienza University of Rome

Mario Polidoro

University of Perugia

L Seganti

Sapienza University of Rome

Catia Longhi

Sapienza University of Rome

Miriam Conte

Sapienza University of Rome

Giovanna Donnarumma

University of Campania "Luigi Vanvitelli"

Paolo Visca

Roma Tre University

Vincenzo Buonfiglio

Casa di Cura Città di Roma

Maria Pia Conte

Sapienza University of Rome

Cooperative Institutions

Sapienza University of Rome

Czech Academy of Sciences, Institute of Microbiology

Istituto Superiore di Sanità

Policlinico Umberto I

University of Naples Federico II

University of Padua

University of Rome Tor Vergata

University of Campania "Luigi Vanvitelli"

National Interuniversity Consortium of Materials Science and Technology

Novo Nordisk (Italy)

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Identification of Listeria monocytogenes by colony hybridization test using the virulence-associated hly and inlA genes as probes.

PubMed (1997)

G. Petrone Mario Polidoro Giovanna Donnarumma Miriam Conte E Papi

We developed a method of identification of Listeria monocytogenes based on colony hybridization with nonradioactively labeled DNA probes, represented by the hly and inlA virulence-associated genes. The procedure described in this paper results simple, rapid, specific and reproducible. Since it can be performed in a short time, the above technique can be applied to detect L. monocytogenes from different source and constitutes a noteworthy and alternative tool to identify this gram-positive pathogenic bacterium.

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Iron availability affects entry of Listeria monocytogenes into the enterocytelike cell line Caco-2

Infection and Immunity (1996)

Maria Pia Conte Catia Longhi Mario Polidoro G. Petrone Vincenzo Buonfiglio

The influence of iron on the entry of Listeria monocytogenes into Caco-2 cells was studied. Iron availability was found to modify the surface hydrophobicity and protein profile of L. monocytogenes, with the result that cell invasion strongly increased upon bacterial growth in iron-rich medium. The enhanced invasive capability of iron-overloaded L. monocytogenes cells correlates to the higher-level expression of the inlAB virulence genes, which were positively iron regulated at the transcriptional level.

Caco-2

10.1128/iai.64.9.3925-3929.1996

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Citations (45)