Ryugo Tero

Toyohashi University of Technology

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Tsuneo Urisu

Nagoya University

Toru Ujihara

Nagoya University

Nobuo Misawa

Azabu University

Yoshiyuki Suda

Toyohashi University of Technology

Toshinori Motegi

National Institutes for Quantum and Radiological Science and Technology

Yasuhiro Iwasawa

RIKEN

T. Urisu

Institute for Molecular Science

Hidetaka Uno

Nagoya University

Hirofumi Takikawa

Toyohashi University of Technology

Masafumi Ito

Japanese Red Cross Nagoya Daiichi Hospital

Cooperative Institutions

Nagoya University

The University of Tokyo

Tohoku University

Osaka University

Japan Science and Technology Agency

Toyohashi University of Technology

National Institute of Advanced Industrial Science and Technology

Kyoto University

Institute for Molecular Science

Kyushu University

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Immobilization of protein molecules on step-controlled sapphire surfaces

Surface Science (2007)

Ryosuke Aoki Taro Arakawa Nobuo Misawa Ryugo Tero T. Urisu

Avidin

Streptavidin

10.1016/j.susc.2007.08.018

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Citations (17)

Incubation type Si-based planar ion channel biosensor

Analytical and Bioanalytical Chemistry (2008)

Tsuneo Urisu Toshifumi Asano Zhenlong Zhang Hidetaka Uno Ryugo Tero

Pipette

Biochip

10.1007/s00216-008-1994-z

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Citations (17)

Imaging Characterization of Cluster-Induced Morphological Changes of a Model Cell Membrane

The Journal of Physical Chemistry C (2016)

Hideki Nabika Aya Sakamoto Toshinori Motegi Ryugo Tero Daiki Yamaguchi

Understanding the activity of nanomaterials at the lipid bilayer surface can provide key information for the feasible design of functional bioactive agents. Herein, we used micro- and nanoscopic imaging techniques to evaluate the activity of nanometer-sized inorganic clusters and report that destruction of the lipid membrane is induced by a cluster-induced morphological change on the membrane surface. As model experiments, we used the Keggin-type polyoxometalate (POM) SiW12O404– for the inorganic cluster and a 1,2-dimyristoyl-sn-glycerol-3-phosphatidylcholine (DMPC) and egg phosphatidylcholine (EPC) bilayer for the cell membrane. Imaging experiments revealed vigorous desorption of the lipid bilayer from solid substrate by the formation of POM–lipid assembly through a supramolecular-type assembly process in which electrostatic and hydrophobic interactions between the POM and lipid determine the efficiency and dynamics of assembly formation and thereby determine lipid desorption. Furthermore, maximum efficiency of lipid desorption was found at the phase-transition temperature. This phase dependency was explained by the formation of a "leaky interface" between the gel and fluid domains, in which freedom in the conformational change of lipids during the formation of the POM–lipid assemblies becomes maximal.

Model lipid bilayer

10.1021/acs.jpcc.5b08014

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Citations (18)

Substrate-Induced Structure and Molecular Dynamics in a Lipid Bilayer Membrane

Langmuir (2017)

Toshinori Motegi Kenji Yamazaki T. Ogino Ryugo Tero

The solid-substrate-dependent structure and dynamics of molecules in a supported lipid bilayer (SLB) were directly investigated via atomic force microscopy (AFM) and single particle tracking (SPT) measurements. The appearance of either vertical or horizontal heterogeneities in the SLB was found to be strongly dependent on the underlying substrates. SLB has been widely used as a biointerface with incorporated proteins and other biological materials. Both silica and mica are popular substrates for SLB. Using single-molecule dynamics, the fluidity of the upper and lower membrane leaflets was found to depend on the substrate, undergoing coupling and decoupling on the SiO2/Si and mica substrates, respectively. The anisotropic diffusion caused by the locally destabilized structure of the SLB at atomic steps appeared on the Al2O3(0001) substrate because of the strong van der Waals interaction between the SLB and the substrate. Our finding that the well-defined surfaces of mica and sapphire result in asymmetry and anisotropy in the plasma membrane is useful for the design of new plasma-membrane-mimetic systems. The application of well-defined supporting substrates for SLBs should have similar effects as cell membrane scaffolds, which regulate the dynamic structure of the membrane.

Dissipative particle dynamics

10.1021/acs.langmuir.7b03212

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Citations (26)

Lipid Membrane Formation by Vesicle Fusion on Silicon Dioxide Surfaces Modified with Alkyl Self-Assembled Monolayer Islands

Langmuir (2004)

Ryugo Tero Morio Takizawa Yanjun Li Masahito Yamazaki Tsuneo Urisu

Using atomic force microscopy, we have investigated the formation of the dipalmitoylphosphatidylcholine (DPPC) membrane by the vesicle fusion method on SiO2 surfaces modified with self-assembled monolayer (SAM) islands of octadecyltrichlorosilane (OTS) with sizes comparable to those of the vesicles. OTS-SAM islands with various sizes and coverages can be constructed on the SiO2 surfaces prepared by thermal oxidation followed by partial hydroxylation in a H2O2/H2SO4 solution. When vesicles are sufficiently smaller than the SiO2 domains, DPPC bilayers and DPPC/OTS layers form on the SiO2 and OTS domains, respectively. However, the adhesion of larger vesicles onto SiO2 is prevented by the OTS islands; therefore only DPPC/OTS layers form without formation of DPPC bilayers on the SiO2 domains. On surfaces with domains on the scale of tens to hundreds of nanometers, the relative size between the hydrophilic domains and the vesicles becomes an important factor in the membrane formation by the fusion of vesicles.

Dipalmitoylphosphatidylcholine

Octadecyltrichlorosilane

Vesicle fusion

Silicon dioxide

10.1021/la0400306

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Citations (43)

Oxygen adsorption states on Mo() surface studied by HREELS

Surface Science (2002)

Takehiko Sasaki Yoshio Gotō Ryugo Tero Kenichi Fukui Yasuhiro Iwasawa

10.1016/s0039-6028(01)01919-7

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Citations (27)

Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers

Materials (2012)

Ryugo Tero

Supported lipid bilayers are artificial lipid bilayer membranes existing at the interface between solid substrates and aqueous solution. Surface structures and properties of the solid substrates affect the formation process, fluidity, two-dimensional structure and chemical activity of supported lipid bilayers, through the 1–2 nm thick water layer between the substrate and bilayer membrane. Even on SiO2/Si and mica surfaces, which are flat and biologically inert, and most widely used as the substrates for the supported lipid bilayers, cause differences in the structure and properties of the supported membranes. In this review, I summarize several examples of the effects of substrate structures and properties on an atomic and nanometer scales on the solid-supported lipid bilayers, including our recent reports.

Model lipid bilayer

Lipid bilayer mechanics

10.3390/ma5122658

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Citations (136)

Si-based planer type ionchannel biosensor

Nanomedicine Nanotechnology Biology and Medicine (2006)

Hidetaka Uno Z-L Zhang T-Y Chiang Koichi SUZUI Ryugo Tero

10.1016/j.nano.2006.10.056

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Citations (0)

Evaluation of the surface induced potential derived from a substrate in supported lipid bilayer using voltage-sensitive dye