Magdalena Gabig‐Cimińska

Gdańsk Medical University

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Joanna Jakóbkiewicz‐Banecka

Gdańsk Medical University

Grzegorz Węgrzyn

University of Gdańsk

Marta Moskot

Gdańsk Medical University

Alicja Węgrzyn

University of Gdańsk

Anna Kłoska

Bydgoszcz University of Science and Technology

Bogdan Banecki

University of Gdańsk

Marcelina Malinowska

Gdańsk Medical University

Elwira Smolińska

Gdańsk Medical University

Katarzyna Bocheńska

Gdańsk Medical University

Sven‐Olof Enfors

KTH Royal Institute of Technology

Cooperative Institutions

Gdańsk Medical University

University of Gdańsk

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Polish Academy of Sciences

KTH Royal Institute of Technology

Fraunhofer Institute for Silicon Technology

Universität Hamburg

MRC Laboratory of Molecular Biology

University of Birmingham

Instytut Oceanologii Polskiej Akademii Nauk

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The model homologue of the partially defective human 5,10-methylenetetrahydrofolate reductase, considered as a risk factor for stroke due to increased homocysteine level, can be protected and reactivated by heat shock proteins

Metabolic Brain Disease (2016)

Michał Grabowski Bogdan Banecki Leszek Kadziński Joanna Jakóbkiewicz‐Banecka Magdalena Gabig‐Cimińska

The A222 V substitution in the human MTHFR gene product (5,10-methylenetetrahydrofolate reductase) is responsible for a decreased activity of this enzyme. This may cause an increased homocysteine level, considered as a risk factor for arteriosclerosis and stroke. The bacterial homologue of the human enzyme, MetF, has been found to be a useful model in genetic and biochemical studies. The similarity of Escherichia coli MetF and human MTHFR proteins is so high that particular mutations in the corresponding human gene can be reflected by the bacterial mutants. For example, the A222 V substitution in MTHFR (caused by the C667T substitution in the MTHFR gene) can be ascribed to the A117 V substitution in MetF. Here, it is reported that a temperature-sensitive MetF117 (A117 V) protein can be partially protected from a thermal inactivation by the heat shock proteins from the Hsp70/100 systems. Moreover, activity of the thermally denatured enzyme can be partially restored by the same heat shock proteins. High temperature protein G (HtpG) had no effect on MetF117 activity in both experimental systems. The presented results indicate that functions of heat shock proteins may be required for maintenance of the MetF117 function. This may have implications for the mechanisms of arteriosclerosis and stroke, especially in the light of previous findings that the A222 V MTHFR polymorphism may be a risk factor for stroke, as well as recently published results which demonstrated the increased levels of antibodies against heat shock proteins in stroke patients.

HSP60

10.1007/s11011-016-9844-8

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Citations (3)

Female Fabry disease patients and X-chromosome inactivation

Gene (2017)

Patrycja Juchniewicz Anna Kłoska Anna Tylki‐Szymańska Joanna Jakóbkiewicz‐Banecka Grzegorz Węgrzyn

Lysosomal storage disease

Heterozygote advantage

Skewed X-inactivation

Asymptomatic carrier

10.1016/j.gene.2017.10.064

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Citations (47)

Metal and antibiotic resistance of bacteria isolated from the Baltic Sea.

PubMed (2012)

Marta Moskot Ewa Kotlarska Joanna Jakóbkiewicz‐Banecka Magdalena Gabig‐Cimińska Karolina Fari

Spectinomycin

Neomycin

Kanamycin

10.2436/20.1501.01.166

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Citations (23)

Recent advances on an electric silicon based chip for analysis of nucleic acids

Magdalena Gabig‐Cimińska Grzegorz Węgrzyn Sven‐Olof Enfors

Recently, several innovative designs for nucleic acid-based electrochemical sensing have appeared. These types of sensors combine nucleic acid layers with electrochemical transducers to produce a biochip.

Source

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Genetic variation in C-reactive protein (CRP) gene is associated with retinopathy and hypertension in adolescents with type 1 diabetes

Cytokine (2022)

Bartosz Słomiński Martyna Jankowiak Agata Maciejewska Maciej Studziński Aleksandra Mączyńska

Genetic predisposition

10.1016/j.cyto.2022.156025

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Citations (1)

Impact of isoflavone genistein on psoriasis in in vivo and in vitro investigations

Scientific Reports (2021)

Katarzyna Bocheńska Marta Moskot Elwira Smolińska-Fijołek Joanna Jakóbkiewicz‐Banecka Aneta Szczerkowska‐Dobosz

Abstract Genistein is applied worldwide as an alternative medicament for psoriasis (Ps) because of its anti-inflammatory activity and perceived beneficial impact on the skin. Hereby, we report our in vivo and in vitro investigations to supplement scientific research in this area. The reduction of clinical and biochemical scores in mild to moderate Ps patients taking genistein, its safety, good tolerability with no serious adverse events or discontinuations of treatment, no dose-limiting toxicities, negligible changes in pharmacodynamic parameters and remarkable serum interleukin level alterations were documented in this study. A certain regression of the Ps phenotype was visible, based on photo-documented Ps lesion evaluation. Through in vitro experiments, we found that genistein reduced IL-17A and TNF-α induced MAPK, NF-κB, and PI3K activation in normal human epidermal keratinocytes. Moreover, at the mRNA level of genes associated with the early inflammatory response characteristic for Ps ( CAMP , CCL20 , DEFB4A , PIK3CA , S100A7 , and S100A9 ) and key cellular signalling ( MTORC1 and TFEB ), we showed that this isoflavone attenuated the increased response of IL-17A- and TNF-α-related pathways. This allows us to conclude that genistein is a good candidate for Ps treatment, being attractive for co-pharmacotherapy with other drugs.

Tolerability

10.1038/s41598-021-97793-4

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Citations (14)

Genistein inhibits activities of methylenetetrahydrofolate reductase and lactate dehydrogenase, enzymes which use NADH as a substrate

Biochemical and Biophysical Research Communications (2015)

Michał Grabowski Bogdan Banecki Leszek Kadziński Joanna Jakóbkiewicz‐Banecka Rajmund Kaźmierkiewicz

10.1016/j.bbrc.2015.08.004

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Genistein: a natural isoflavone with a potential for treatment of genetic diseases

Biochemical Society Transactions (2010)

Grzegorz Węgrzyn Joanna Jakóbkiewicz‐Banecka Magdalena Gabig‐Cimińska Ewa Piotrowska Magdalena Narajczyk

Genistein [4′,5,7-trihydroxyisoflavone or 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one] is a natural isoflavone occurring in many plants known to possess various biological activities, ranging from phyto-oestrogenic to antioxidative actions. Recent studies indicated that this isoflavone can also be considered as a drug for as yet untreatable genetic diseases. In the present review, we discuss a plausible use of genistein in treatment of two genetic disorders: CF (cystic fibrosis) and MPS (mucopolysaccharidosis). Although various biological actions of genistein are employed in these two cases, in vitro studies, tests on animal models and pilot clinical trials suggest that this plant-derived compound might be a real hope for patients suffering from severe inherited disorders with relatively complicated pathomechanisms, including those affecting the central nervous system.

10.1042/bst0380695

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Citations (52)

Keratinocyte-Mediated Antigen Presentation in Psoriasis: Preliminary Insights from In Vitro Studies

International Journal of Molecular Sciences (2024)

Katarzyna Zima Dorota Purzycka-Bohdan Aneta Szczerkowska‐Dobosz Magdalena Gabig‐Cimińska

Antigen presentation plays a critical role in the pathogenesis of immune-mediated disorders. This study aimed to investigate the effects of IFN-γ and a cytokine mix (5MIX: IL-1α, IL-17A, IL-22, OsM, and TNF-α) on the antigen-presenting capabilities of keratinocytes, with a specific focus on immune-mediated dermatological conditions such as psoriasis (Ps). To achieve this, keratinocytes were treated with IFN-γ and 5MIX, and their impact on the expression of key antigen-presentation molecules, HLA-DRα and CD74, was assessed. Transcriptomic analysis revealed that IFN-γ alone altered the expression of 254 genes, highlighting its central role in modulating immune responses, including the recruitment of immune cells and regulation of inflammation. Temporal experiments further demonstrated that IFN-γ and 5MIX enhanced early endocytic activity and lysosomal degradation pathways, both essential for effective antigen presentation and T-cell activation. To extend these findings to a clinical context, a co-culture model using keratinocytes derived from psoriatic patients was established. This model revealed increased cytokine production following antigen stimulation, indicating robust and consistent CD4+ and naïve T-cell responses. These results elucidate the complex dynamics of cytokine signaling and antigen presentation in keratinocytes, providing insights into potential therapeutic strategies for immune-mediated skin disorders like Ps.

Antigen processing

Proinflammatory cytokine

10.3390/ijms252413387

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Citations (0)

ClpP/ClpX-mediated degradation of the bacteriophage λ O protein and regulation of λ phage and λ plasmid replication

Archives of Microbiology (2000)

Alicja Węgrzyn Agata Czyż Magdalena Gabig‐Cimińska Grzegorz Węgrzyn

Lytic cycle

Lysogenic cycle

Lambda phage

Wild type

10.1007/s002030000177

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Citations (23)