Shuanghu Cai

Guangdong Ocean University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jichang Jian

Guangdong Ocean University

Yucong Huang

Guangdong Ocean University

Shiping Yang

Guangdong Ocean University

Yishan Lu

Guangdong Ocean University

Zaohe Wu

Guangdong Ocean University

Yunhao Yuan

Guangdong Ocean University

Junliang Luo

Guangdong Ocean University

Yongxiong Huang

Guangdong Provincial People's Hospital

Bei Wang

Guangdong Ocean University

Huanying Pang

Guangdong Ocean University

Cooperative Institutions

Guangdong Ocean University

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)

Qingdao National Laboratory for Marine Science and Technology

Chinese Academy of Sciences

Sun Yat-sen University

Key Laboratory of Guangdong Province

Dalian Ocean University

Zhanjiang Experimental Station

University of Hong Kong

Shanghai Jiao Tong University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Characterization and expression analysis of B Cell receptor accessory molecule CD79 gene in humphead snapper (Lutjanus sanguineus)

Journal of Ocean University of China (2016)

Yucong Huang Xiuying Yan Shuanghu Cai Jia Cai Jichang Jian

10.1007/s11802-016-2816-0

Cite

Citations (4)

The role of dctP gene in regulating colonization, adhesion and pathogenicity of Vibrio alginolyticus strain HY9901

Journal of Fish Diseases (2021)

Yilin Zhang Huimin Tan Shiping Yang Yucong Huang Shuanghu Cai

Vibriosis caused by Vibrio alginolyticus has severely affected the development of mariculture industry in recent decades. DctP, a tripartite ATP-independent periplasmic transporter solute-binding subunit, is thought to be one of the virulence factors in Vibrio. In this study, the results displayed no difference in morphological characteristics and growth between ΔdctP (dctP mutant strain) and WT (wild-type strain). Nevertheless, the ability of swarming motility, biofilm formation, ECPase formation, cell adhesion and colonized ability of ΔdctP significantly decreased compared to those of WT. The LD50 of ΔdctP significantly increased by 40-fold compared to that of WT. The transcriptome analysis demonstrated the deletion mutation of dctP could regulate the expression levels of 22 genes related to colonization, adhesion and pathogenicity in V. alginolyticus. The analysis of qRT-PCR showed the transcriptome data were reliable. These results reveal the effect of attenuated function of DctP on colonization, adherence and pathogenicity by controlling the expression of related gene.

Vibrio alginolyticus

10.1111/jfd.13571

Cite

Citations (9)

Identification and Characterization of four Bacillus species from the intestine of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂), their antagonistic role on common pathogenic bacteria, and effects on intestinal health

Fish & Shellfish Immunology (2024)

Kwaku Amoah Jia Cai Yü Huang Bei Wang Vicent Michael Shija

Epinephelus

Identification

Pathogenic bacteria

Bacillus (shape)

10.1016/j.fsi.2024.109795

Cite

Citations (3)

Biological characterisation, expression and functional analysis of non-specific cytotoxic cell receptor protein 1 in Nile tilapia (Oreochromis niloticus)

Fish & Shellfish Immunology (2020)

Yu Huang Xinchao Liu Jia Cai Jufen Tang Shuanghu Cai

Nile tilapia

10.1016/j.fsi.2020.05.048

Cite

Citations (11)

Vibrio harveyi (VirB11) recombinant vaccine development against vibriosis in orange‐spotted grouper ( Epinephelus coioides )

Aquaculture Research (2019)

Denicia Atujona Yucong Huang Zhiwen Wang Jichang Jian Shuanghu Cai

Despite significant improvements in aquaculture to compensate wild catch, disease organisms have thrived in limiting its national and global potential. Using antibiotics, in a bid to remedy the havoc, has given rise to complications, attracting attention to disease prevention by immune enhancement against diseases. Grouper production has been inhibited for the threats of bacterial infection, particularly of Vibrio origin. Considering the rise in vibriosis cases, improved vaccines are necessary; moreover, recombinant vaccines, the choice for trial in the present experiment have been effective and more specific in improving immunity. The current work deals with grouper immune system enhancement with a recombinant vaccine developed from VirB11 gene in Vibrio harveyi. VirB11 was cloned in V. harveyi for recombinant vaccine development against vibriosis in orange-spotted grouper (Epinephelus coioides). As indicated by the results, recombinant VirB11 protein showed effectiveness in conferring protection against vibriosis with observable specific antibody response in enzyme-linked immunosorbent assay (ELISA) analysis; a significant increase (p < 0.05) in antibody levels was observed after a week and after 8 weeks post-vaccination. From the weeks post-vaccination, log2 (antibody titres) in the sera of vaccinated groups reached a peak of 14.2 at week 5 in the vaccinated group in comparison with a peak of approximately 5 and 2 in adjuvant and PBS controls. As indicated by the challenge results, 90% relative survival was observed in vaccinated group and 13% relative survival in control group I (adjuvant control). The cumulative performance of protein concludes VirB11 commendable for recombinant vaccine development.

Epinephelus

Vibrio harveyi

10.1111/are.14220

Cite

Citations (9)

Molecular Characterization and Bioinformatics Analysis of Virb11 in <i>Vibrio Harveyi</i>

Genomics and Applied Biology (2018)

Denicia Atujona Weijian Liang Shuanghu Cai Huanying Pang Umar Farouk Mustapha

Vibrio harveyi has caused devastating loses in mariculture through vibrioses infection. VirB11 plays a critical role in bacteria substrate translocation and virulence. The present study is a bioinformatic analysis of VirB11 in Vibrio harveyi . VirB11 was cloned and analyzed, unveiling an encoded nucleotide sequence of 1,034 bp coding 338 amino acids. A molecular weight and pI of 37,703.39 Da and 6.31 were respectively predicted. No signal peptides and transmembranes were predicted. A conserved P-loop NTPase was predicted, multiple predictions also included 3D module.

Vibrio harveyi

Mariculture

10.5376/gab.2018.09.0008

Cite

Citations (0)

Expression and immunogenicity analysis of accessory colonization factor A from Vibrio alginolyticus strain HY9901

Fish & Shellfish Immunology (2012)

Shuanghu Cai Yucong Huang Yishan Lu Zaohe Wu Bei Wang

Vibrio alginolyticus

10.1016/j.fsi.2012.11.051

Cite

Citations (15)

Mini review on Vibrio Infection-A Case Study on Vibrio harveyi Clade

Fisheries and Aquaculture Journal (2018)

Denicia Atujona Shuanghu Cai Eric Amenyogbe

Vibrio harveyi

10.4172/2150-3508.1000258

Cite

Citations (2)

A live attenuated strain of HY9901ΔdctP provides protection against Vibrio alginolyticus to the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Frontiers in Marine Science (2022)

Yilin Zhang Ziyu Zhang Jinxia Zhang Yucong Huang Jichang Jian

In recent decades, vibriosis caused by Vibrio alginolyticus has become a severe threat to the global mariculture industry. There is an urgent need for an effective vaccine to alleviate this unoptimistic situation. In this study, we evaluated the safety, immunoprotection, and specific and non-specific immune response effect of Δ dctP strain as a live-attenuated vaccine to pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu ). The results demonstrate that the safe dose of Δ dctP was ≤1.0 ×10 6 CFU in pearl gentian grouper. The relative percent survival of the pearl gentian grouper challenged with the Δ dctP mutant strain by intraperitoneal injection reached 74.4%, which was significantly higher than that of the control group. Meanwhile, the expression level of immune-relative genes, including IgM , IL-1β , IL-8 , IL-10 , MHC-Iα , MHC2 , TNF-α , TLR3 , and CD4 , were upregulated in liver, spleen, and head kidney within 28 d post-vaccination. Moreover, specific antibody IgM, total serum protein as well as activities of catalase, superoxide dismutase, and lysozyme in serum were significantly up-regulated in vaccinated groupers compared with those in control. Collectively, Δ dctP could be used as a live-attenuated vaccine candidate against V. alginolyticus infection in pearl gentian grouper.

Vibrio alginolyticus

Epinephelus

Attenuated vaccine

10.3389/fmars.2022.907407

Cite

Citations (1)

Characterization and function analysis of interleukin-1 receptor-associated kinase-1 (IRAK-1) from Fenneropenaeus penicillatus

Fish & Shellfish Immunology (2016)

Youhou Xu Yucong Huang Shuanghu Cai

Vibrio alginolyticus

White spot syndrome

Protein kinase domain

10.1016/j.fsi.2016.12.030

Cite

Citations (14)