A synthetic probe, STR 14C13, detects a new polymorphic locus on chromosome arm 7q (D7S450) Get access Valérie Lauthier, Valérie Lauthier Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance Search for other works by this author on: Oxford Academic PubMed Google Scholar Denis Mariat, Denis Mariat Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance Search for other works by this author on: Oxford Academic PubMed Google Scholar Monique Zoroastro, Monique Zoroastro Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance Search for other works by this author on: Oxford Academic PubMed Google Scholar Gilles Vergnaud Gilles Vergnaud * Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 14, 25 July 1991, Page 4014, https://doi.org/10.1093/nar/19.14.4014-a Published: 25 July 1991
A synthetic probe, STR 16C2, detects a new polymorphic locus at 5pter (D5S206) Get access Valérie Lauthier, Valérie Lauthier Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Denis Mariat, Denis Mariat Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Monique Zoroastro, Monique Zoroastro Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Gilles Vergnaud Gilles Vergnaud * Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 14, 25 July 1991, Page 4013, https://doi.org/10.1093/nar/19.14.4013 Published: 25 July 1991
A synthetic probe, STR 16018, detects a new polymorphic locus at 12qter (Dl2S55) Valérie Lauthier, Valérie Lauthier Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Denis Mariat, Denis Mariat Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Monique Zoroastro, Monique Zoroastro Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Gilles Vergnaud Gilles Vergnaud * Centre d'Etudes du Bouchet, Laboratoire de Génétique MoléculaireBP no. 3, 91710 Vert le Petit, France *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 14, 25 July 1991, Page 4013, https://doi.org/10.1093/nar/19.14.4013-a Published: 25 July 1991
Loci containing tandem repeats of short sequences are sometimes associated with a high level of polymorphism due to variations in the number of repeats. The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redundant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.
Journal Article A synthetic probe, STR 16C17, detects a new polymorphic locus at l7pter (D17S450) Get access Valérie Lauthier, Valérie Lauthier Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance Search for other works by this author on: Oxford Academic PubMed Google Scholar Denis Mariat, Denis Mariat Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance Search for other works by this author on: Oxford Academic PubMed Google Scholar Monique Zoroastro, Monique Zoroastro Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance Search for other works by this author on: Oxford Academic PubMed Google Scholar Gilles Vergnaud Gilles Vergnaud * Centre d'Etudes du Bouchet, Laboratoire de Génétique Moléculaire, BP no. 3, 91710 Vert le PetitFrance *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 14, 25 July 1991, Page 4014, https://doi.org/10.1093/nar/19.14.4014 Published: 25 July 1991
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