Enrico Berger

Brandenburg University of Technology Cottbus-Senftenberg

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Rico Hiemann

Brandenburg University of Technology Cottbus-Senftenberg

Dirk Roggenbuck

Brandenburg University of Technology Cottbus-Senftenberg

Peter Schierack

Brandenburg University of Technology Cottbus-Senftenberg

Lisa K. Nolan

University of Georgia

Alexander Swidsinski

Charité - Universitätsmedizin Berlin

Bryon Nicholson

Boehringer Ingelheim (United States)

Rafał Kolenda

Quadram Institute

Dirk Reinhold

Otto-von-Guericke University Magdeburg

Christoph Kuhl

Stefan Rödiger

Brandenburg University of Technology Cottbus-Senftenberg

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Brandenburg University of Technology Cottbus-Senftenberg

University of Potsdam

Charité - Universitätsmedizin Berlin

Freie Universität Berlin

Iowa State University

Ames National Laboratory

TU Dresden

Humboldt-Universität zu Berlin

Wroclaw University of Environmental and Life Sciences

University of Veterinary Medicine

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Schierack et al Gut 2014 GP2 ab suppl

Peter Schierack Stefan Rödiger Rafał Kolenda Rico Hiemann Enrico Berger

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Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2)

Gut (2014)

Peter Schierack Stefan Rödiger Rafał Kolenda Rico Hiemann Enrico Berger

Dear Editor, With interest we read the paper by Juste et al 1 proposing the amount of zymogen-granule membrane glycoprotein 2 (GP2) on the surface of intestinal bacteria as a Crohn's disease (CD) marker. Indeed, a decreased GP2 level was found on microbes in patients with CD as compared to those of healthy controls. GP2 is a homologue to the urinary Tamm–Horsefall protein demonstrating an antimicrobial function by binding type 1-fimbriated uropathogenic Escherichia coli (UPEC). Likewise, GP2 seems to interact with intestinal bacteria as a specific receptor of bacterial type-1 fimbriae (FimH) on intestinal microfold cells that are partaking in immune responses against such microbes.2 GP2 is overexpressed in the inflamed intestine of patients with CD and has an immunomodulating role in innate and acquired immune responses.3 ,4 Interestingly, GP2 was identified as autoantigen of pancreatic antibodies in CD.4 Altogether, these findings indicate two major GP2 sources (pancreatic/intestinal) and support a role for GP2 in the interaction between the immune system and intestinal microbiota.3 Thus, loss of tolerance to GP2 could play a role in CD's pathophysiology supposed to be exacerbated by preceding intestinal infections. In general, the findings by Juste et al 1 may be explained by a lower pancreatic GP2 secretion, an impaired GP2 binding to bacteria, or by a higher prevalence of bacteria with poor or no GP2 binding in patients with CD. We have a longstanding interest in GP2's intestinal function and, therefore, we evaluated GP2 as receptor for intestinal bacterial pathotypes by testing the binding of 284 bacteria in a …

10.1136/gutjnl-2014-307854

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[Incidence of pathogenic coli strains in enteritis in infants].

PubMed (1955)

Enrico Berger Michael T. Vest

Pathogenic Escherichia coli

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Association of adhesion and a probiotic effect.

Peter Schierack Stefan Rödiger Christoph Kuhl Rico Hiemann Dirk Roggenbuck

Association (psychology)

10.1371/journal.pone.0059242.g004

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Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method

PLoS ONE (2013)

Peter Schierack Stefan Rödiger Christoph Kuhl Rico Hiemann Dirk Roggenbuck

We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.

Virulence factor

10.1371/journal.pone.0059242

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Citations (15)