Mukesh Kumar

Chaudhary Charan Singh Haryana Agricultural University

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Vikram Singh

Chaudhary Charan Singh Haryana Agricultural University

Lakshmi Chaudhary

Chaudhary Charan Singh Haryana Agricultural University

Shikha Yashveer

Chaudhary Charan Singh Haryana Agricultural University

Vijeta Gupta

Chaudhary Charan Singh Haryana Agricultural University

R. Sharma

Oklahoma State University

Kavita Lamba

Chaudhary Charan Singh Haryana Agricultural University

D. V. Pathak

Chaudhary Charan Singh Haryana Agricultural University

Virender Singh Mor

Chaudhary Charan Singh Haryana Agricultural University

Meenakshi Rathi

Centre for Development of Advanced Computing

R. S. Sengar

Sardar Vallabhbhai Patel University of Agriculture & Technology

Cooperative Institutions

Chaudhary Charan Singh Haryana Agricultural University

158

Indian Agricultural Research Institute

Sardar Vallabhbhai Patel University of Agriculture & Technology

Indian Council of Agricultural Research

Banaras Hindu University

Institute of Medical Sciences

Chandra Shekhar Azad University of Agriculture and Technology

Narendra Dev University of Agriculture and Technology

University of Delhi

Maharaja Sayajirao University of Baroda

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Multi-centric clinic trials in evidence-based research – A narrative review on the Indian scenario

Journal of Family Medicine and Primary Care (2023)

Deepa Jatti Patil Tarun Vyas Ajay Pal Singh Kataria Rajan Rajput Albert Ashem

Evidence-based health care is gaining prominence since the past many decades. The ultimate goal of evidence-based medicine is providing the best available treatment to patients. The boom in pharmacy sector has seen a rapid rise in randomised controlled clinical trials. Novel medicines or diagnostic tests must be tested before introducing to the target population. Randomised controlled trials are at the top hierarchy of evidence-based health care, especially for testing newly invented drugs. The results obtained from randomised controlled trials cannot be generalised to the entire population. This has led to the evolvement of multi-centre trials in evidence-based research. Multi-centre trials can overcome the barriers associated with single-centre clinical trials. The conduct of multi-centre trials is still in a budding stage in India. Although there are many ongoing multi-centre trials in India, very few trials are conducted among the dental fraternity. The conduct of such trials has its own set of challenges involving funding, ethical committee approval, and logistic requirements. This paper will discuss the growth of multi-centre research, steps involved in conduct of multi-centre trials, and the challenges faced in conducting these trials by the dental specialists in India.

10.4103/jfmpc.jfmpc_2257_22

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Use of an rRNA internal transcribed spacer region to distinguish closely related isolates of the genera Rhizoctonia

Indian Journal of Genetics and Plant Breeding (The) (2008)

D. Monga Mukesh Kumar P. K. Chakrabarty

Inter-and intra specific variation among isolates of Rhizoctonia bataticola and R. solani the causal organism of root rot of cotton were evaluated by analysis of the internal transcribed spacer (ITS) sequences of the rRNA region. The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and amplification products, which ranged between 540 to 680 bp were obtained for all the isolates analyzed.The degree of polymorphism observed did not allow proper identification of most of the isolates. Analysis of two internal transcribed spacer sequences ITS 1 and ITS 2, revealed that within the isolates of R. bataticola, the size of ITS 1 region ranged from 135 to 184 bp while the size of ITS 2 varied by 4 bp. The size of ITS 1 ranged from 155 to 205 bp, while the size of ITS 2 ranged from 149 to 251 bp in isolates of R. solani. Based on phylogenetic analysis of the ITS 1 and ITS2 sequences different isolates of R. bataticola and R. solani were grouped in separate clusters.

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Microbial Inoculants as Biofertilizers and Biopesticides

Springer eBooks (2016)

D. V. Pathak Mukesh Kumar

Biofertilizer

Biopesticide

Azotobacter

10.1007/978-81-322-2647-5_11

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Citations (48)

SSR Markers based Molecular Assessment of Stripe Rust Resistance Genes in F3 Population of Cross DBW17 x WH1105 of Wheat (Triticum aestivum L.)

International Journal of Current Microbiology and Applied Sciences (2020)

Vijeta Gupta Mukesh Kumar Vikram Singh Lakshmi Chaudhary

To evaluate the presence of resistance genes parents (DBW17 and WH1105) and F3 population of cross DBW17 x WH1105 were screened for reaction to stripe rust using Modified Cobb’s Scale and using SSR markers. Considering the inheritance pattern of resistance to yellow rust a ratio of 13:3 was observed for resistant to susceptible plants. For genotyping 99 primers were used out of which 15 primers were found to be polymorphic and were dispersed over the wheat genome (AABBDD), with allele range 2-5. A total of 32 alleles were detected in polymorphic markers. The average number of SSR alleles per locus was 2.13, with a range from 2 to 4. PIC values of various SSR loci ranged from 0.44 to 0.66. The hierarchical cluster analysis revealed that the F3 population along with their parents were mainly divided into two major clusters, I and II at a similarity coefficient of 0.54. Dendrogram confirmed that two parents were highly diversed and all of the progeny lines were interspersed between the two parents. These parents can be used for developing RIL’s that could be used for QTL mapping to identify the resistance genes and chromosome location.

Dendrogram

Rust (programming language)

10.20546/ijcmas.2020.911.021

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Impact of High Temperature on Germination, Seedling Growth and Enzymatic Activity of Wheat

Agriculture (2022)

Sushma Sharma Vikram Singh Hemender Tanwar Virender Singh Mor Mukesh Kumar

Global warming has increased the temperature significantly over a large extent both spatially and temporally. The threat of heat stress during the germination and seedling establishment stages in the wheat crop is now more prevalent than ever before. The present experiment assessed the effect of elevated temperature on the germination and early seedling growth of wheat genotypes. The seeds were sown under four temperatures, viz., 20 °C, 25 °C, 30 °C and 35 °C; the germination, seedling vigor and enzyme activities in 8-day-old seedlings were assessed. The temperature significantly influenced germination and early seedling growth. The germination percentage at 20 and 25 °C was statistically on par with and higher than at 30 and 35 °C. The seedling vigor parameters were maximum at 25 °C and showed a reduction at higher temperatures. Genotypic differences were observed for early heat stress as the genotypes WH 730, WH 1123 and HD 2967 showed tolerance towards heat stress during germination, whereas the genotypes PBW 725 and WH 1105 were susceptible. Antioxidant enzyme activities in seedlings increased with the rise in temperature. Catalase, peroxidase and superoxide dismutase enzymes showed increased activities at higher temperature levels.

10.3390/agriculture12091500

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Citations (29)

Microsatellites based assessment of genetic diversity and population structure of indian lentil (Lens culinaris Medik.) genotypes

Biologia (2023)

R. Sharma Lakshmi Chaudhary Mukesh Kumar

Jaccard index

Genetic distance

10.1007/s11756-023-01385-8

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The Status of Distance Education in Assam

Mukesh Kumar

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Study on inter relation and path coefficient for yield and its attributing characters in soyabean [Glycine max (L.)].

Journal of Plant Development Sciences (2009)

Mukesh Kumar Vipin Kumar J Singh

Path coefficient

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Molecular characterization of pearl millet [Pennisetum glaucum (L.) R. Br] inbreds using microsatellite markers

Journal of Applied and Natural Science (2017)

Mamta Nehra Mukesh Kumar Dev Vart Jyoti Kaushik Rajesh Kumar Sharma

Studies on genetic diversity in Pennisetum germplasm are the promising opportunities for the use of un-domesticated materials for improving pearl millet varieties. DNA based markers have now emerged as a potential genomic tool for estimation of genetic diversity among various cultivars and varietal identification. In present study, genetic diversity among 49 stay green inbreds of pearl millet was studied using simple sequence repeats (SSRs). Twenty nine polymorphic SSR primers, identified after initial screening of 70, were used to study diversity among these lines. A total of 108 alleles were amplified, collectively yielding unique SSR profiles for all the 49 inbreds. The average number of SSR alleles per locus was 3.72, with a range from 2 to 13. Polymorphic information content (PIC) values of various SSR loci across all the 49 inbreds ranged from 0.14 to 0.87 with an average of 0.51 per lo-cus. This indicated sufficient diversity among the 49 pearl millet inbreds and total 5 out of 29 polymorphic SSR loci, namely Xpsmp2070, Xpsmp2001, Xpsmp2008, Xpsmp2066, Xpsmp2072 revealed PIC values above 0.70, can be considered highly useful for differentiation of pearl millet inbred lines. The lowest PIC value (0.47) for linkage group 7 showed comparatively conserved nature of this linkage group A dendrogram obtained using WARD’s minimum variance method further delineates 49 inbreds into 8 major clusters, and the clustering pattern corroborated with their pedigree and characteristics traits.

Pennisetum

Germ plasm

Dendrogram

10.31018/jans.v9i1.1196

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Evaluation of aonla (Phyllanthus emblica) genotypes under semi-arid conditions

The Indian Journal of Agricultural Sciences (2022)

Narendra Singh Mukesh Kumar Jeet Ram Sharma

The present study was carried out to evaluate the aonla (Phyllanthus emblica L. syn. Emblica officinalis Gaertner) genotypes for their growth, yield and fruit quality attributes under the semi-arid condition. The evaluated genotypes had significant variability for the observed parameters. Chakaiya and BSR 1 were found comparatively less vigorous amongst selected genotypes. The number of male flowers, female flowers and total number of flowers, on 10 cm length of indeterminate branch varied from 650.67 to 1488.3, 4.33 to 12.33 and 657.0 to 1499.0, respectively. Sex ratio (male/female) ranged between 96.04 (NA 6) and 262.28 (S 1). Among the genotypes under investigation, NA 20 gave maximum yield, with higher fruit weight (54.96 g) and pulp per cent (93.87). TSS and TSS: acid ratio was observed higher in BSR 1. Total sugars, reducing sugars and non-reducing sugars content was found highest in genotype Krishna. Ascorbic acid and tannin content was recorded highest in Chakaiya and NA 10, respectively.

Phyllanthus emblica

Emblica officinalis

Tannin

10.56093/ijas.v91i12.120806

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Citations (2)