Pierrick Labbé

Institut des Sciences de l'Evolution de Montpellier

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Mylène Weill

Institut des Sciences de l'Evolution de Montpellier

Pascal Milesi

Uppsala University

Haoués Alout

Animal, Santé, Territoires, Risques et Ecosystèmes

Serge Cosnier

Université Grenoble Alpes

Nicole Pasteur

Université de Montpellier

Patrick Makoundou

Institut des Sciences de l'Evolution de Montpellier

Liliane Coche‐Guérente

Département de Chimie Moléculaire

Arnaud Berthomieu

Centre National de la Recherche Scientifique

Thomas Lenormand

École Pratique des Hautes Études

Benoît Sessinou Assogba

MRC Unit the Gambia

Cooperative Institutions

Centre National de la Recherche Scientifique

208

Université de Montpellier

121

Institut de Recherche pour le Développement

100

Institut des Sciences de l'Evolution de Montpellier

Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle

École Pratique des Hautes Études

University of Oxford

Agropolis International

Inserm

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

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Axisymmetric Elastic-Plastic FE Analysis of Pressurized Elbows

Journal of Pressure Vessel Technology (1995)

Dj. Boussaa K. Dang Van Pierrick Labbé Hehan Tang

Abstract A variational formulation for the pure in-plane bending problem of pressurized elbows with arbitrary cross section, written in the 3-D infinitesimal displacement continuum mechanics framework, and particularly appropriate when a refined elastic-plastic local response is needed (e.g., in the presence of ratcheting), is presented. The similarity between this problem and an axisymmetric analysis is underlined and turned to account to solve the former using existing FE routines devoted to the latter. Some applications are given to illustrate the potentialities of the model.

Section (typography)

10.1115/1.2842136

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Citations (3)

Unraveling the Spatial Distribution of Immunoglobulins, Enzymes, and Polyelectrolytes within Layer-by-Layer Self-Assembled Multilayers. Ellipsometric Studies

Langmuir (2006)

Nancy F. Ferreyra Erica Forzani Manuel López Teijelo Liliane Coche‐Guérente Pierrick Labbé

The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ψ−Δ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.

Ellipsometry

Layer by layer

Glucose oxidase

10.1021/la061173q

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Citations (3)

Human activities and densities shape insecticide resistance distribution and dynamics in the virus-vector Culex pipiens mosquitoes from Morocco

Parasites & Vectors (2024)

Soukaina Arich Najlaa Assaid Mylène Weill Fatim‐Zohra Tmimi Hassan Taki

Abstract Background Mosquitoes of the Culex pipiens complex are widely distributed vectors for several arboviruses affecting humans. Consequently, their populations have long been controlled using insecticides, in response to which different resistance mechanisms have been selected. Moreover, their ecological preferences and broad adaptability allow C. pipiens mosquitoes to breed in highly polluted water bodies where they are exposed to many residuals from anthropogenic activities. It has been observed for several mosquito species that anthropization (in particular urbanization and agricultural lands) can lead to increased exposure to insecticides and thus to increased resistance. The main objective of the present study was to investigate whether and how urbanization and/or agricultural lands had a similar impact on C. pipiens resistance to insecticides in Morocco. Methods Breeding sites were sampled along several transects in four regions around major Moroccan cities, following gradients of decreasing anthropization. The imprint of anthropogenic activities was evaluated around each site as the percentage of areas classified in three categories: urban, agricultural and natural. We then assessed the frequencies of four known resistance alleles in these samples and followed their dynamics in five urban breeding sites over 4 years. Results The distribution of resistance alleles revealed a strong impact of anthropization, in both agricultural and urbanized lands, although different between resistance mutations and between Moroccan regions; we did not find any clear trend in the dynamics of these resistance alleles during the survey. Conclusions Our study provides further evidence for the role of anthropic activities in the selection and maintenance of mutations selected for resistance to insecticides in mosquitoes. The consequences are worrying as this could decrease vector control capacities and thus result in epizootic and epidemic outbreaks. Consequently, concerted and integrated disease control strategies must be designed that include better management regarding the consequences of our activities. Graphical Abstract

Culex pipiens

Entomology

10.1186/s13071-024-06164-1

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Citations (6)

[New technic for determining absorption spectra at low temperature].

PubMed (1969)

Pierrick Labbé Paulette Chaix

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Citations (9)

Évolution de l'adaptation locale : le cas de la résistance aux insecticides chez le moustique "Culex pipiens"

Pierrick Labbé

Insecticide Resistance

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Issue information

Evolution Letters (2017)

Reto Burri Kat Bebbington Sjouke A. Kingma John W. Davey Sarah L. Barker

10.1002/evl3.23

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Multivalent Ion/Polyelectrolyte Exchange Processes in Exponentially Growing Multilayers

Langmuir (2005)

Eric Hübsch Guillaume Fleith Julien Fatisson Pierrick Labbé J.‐C. Voegel

We show, in this paper that multivalent ferrocyanide anions can penetrate into exponentially growing (PGA/PAH)n multilayer films whatever the nature of the last deposited layer. These ions are not able to diffuse out of the film when it is brought in contact with a pure buffer solution. However, the contact of this film with a poly(allylamine) (PAH) or a poly(L-glutamic acid) (PGA) solution leads to the release of ferrocyanide ions from the multilayer. It is shown that the release of ferrocyanide anions, when the film is in contact with a PGA solution, is due to the diffusion of the PGA chains into the film so that an exchange between ferrocyanide ions and PGA chains takes place inside the film. On the other hand, PAH chains do not diffuse into PGA/PAH multilayers. When the film is then brought in contact with a PAH solution, the PAH chains from the solution are expected to strongly interact with the ferrocyanide ions and thus induce a diffusion mechanism of the multivalent anions out of the film, the film/solution interface playing the role of a sink for these ions. This work thus shows that interactions between multivalent ions and exponentially growing films are much more complex than expected at first sight and that polyelectrolyte multilayers must be seen as dynamic entities in which diffusion and exchange processes can take place.

10.1021/la047258d

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Citations (71)

Amplification of Amperometric Biosensor Responses by Electrochemical Substrate Recycling. 3. Theoretical and Experimental Study of the Phenol−Polyphenol Oxidase System Immobilized in Laponite Hydrogels and Layer-by-Layer Self-Assembled Structures

Analytical Chemistry (2001)

Liliane Coche‐Guérente Pierrick Labbé Virginie Mengeaud

The amperometric response toward phenol of PPO-based rotating disk bioelectrodes is analyzed on the basis of a kinetic model taking into account internal and external mass transport effects and a CEC' electroenzymatic mechanism. Monophenolase activity of PPO catalyses the oxidation of phenol to o-quinone (step C). o-Quinone can then enter an amplification recycling process involving electrochemical reduction (step E) and enzymatic reoxidation (step C': catecholase activity). The rate-limiting steps such as monophenolase activity, catecholase recycling, permeability of the membrane, and activity and accessibility of the catalytic enzyme sites are theoretically considered and experimentally demonstrated for different electrode configurations including PPO immobilized in Laponite hydrogels and layer-by-layer self-assembled multilayers of PPO and poly(diallyldimethylammonium).

Amperometry

Catechol

Glucose oxidase

10.1021/ac001534l

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Citations (72)

Cytochrome P-450 reductase is responsible for the ferrireductase activity associated with isolated plasma membranes of

FEMS Microbiology Letters (1997)

Emmanuel Lesuisse Monique Casteras-Simon Pierrick Labbé

10.1016/s0378-1097(97)00418-7

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Citations (8)

Distribution of insecticide resistance and molecular mechanisms involved in the West Nile vector Culex pipiens in Morocco

Pest Management Science (2020)

Soukaina Arich Najlaa Assaid Hassan Taki Mylène Weill Pierrick Labbé

Abstract BACKGROUND Mosquitoes of the Culex pipiens complex are the vectors of several arboviruses and are thus subjected to insecticide control worldwide. However, overuse of insecticides selects for resistance. While assessing the resistance status of the vectors is required for effective and sustainable disease control, resistance has so far only been sparsely studied in Morocco. In this study, we establish a first countrywide assessment of the levels of resistance to various insecticides and the potential responsible mechanisms involved. Cx. pipiens larvae were collected from natural populations of five regions of Morocco, and their taxonomic status was determined (molecular forms). The level of their susceptibility to insecticides was assessed by single‐diagnostic‐dose bioassays. Molecular identification of known resistance alleles was investigated to determine the frequency of target‐site mutations. RESULTS This study confirms that Moroccan populations are an interbreeding mix of pipiens and molestus forms, with large gene flow for the resistance alleles. We also found that Cx. pipiens mosquitoes are resistant to all insecticide families, all over Morocco: resistance is high for insecticides used in mosquito control, but also present for other pesticides. Resistance alleles are similarly more frequent for mosquito control insecticides. However, their distribution is heterogeneous in the five regions, with significant genetic differentiation between populations, revealing the crucial role of local insecticide treatment practices. CONCLUSION This study provides reference countrywide data that highlight the need for further research to refine the distribution of resistance in Morocco and to understand the role of agriculture/urban residuals in its spread. © 2020 Society of Chemical Industry

Culex pipiens

Insecticide Resistance

Pesticide resistance

10.1002/ps.6127

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Citations (8)