Lars Hennig

Friedrich-Alexander-Universität Erlangen-Nürnberg

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Claudia Köhler

Swedish University of Agricultural Sciences

Wilhelm Gruissem

ETH Zurich

Minerva S. Trejo‐Arellano

Swedish University of Agricultural Sciences

Shujing Liu

Nanjing Agricultural University

Rafael Muñoz‐Viana

Instituto de Neurociencias

Lejon E. M. Kralemann

Leiden University

Iva Mozgová

Czech Academy of Sciences, Biology Centre

D. Magnus Eklund

Uppsala University

Yichun Qiu

Max Planck Institute of Molecular Plant Physiology

D. Vivolo

University of Campania "Luigi Vanvitelli"

Cooperative Institutions

Centre National de la Recherche Scientifique

359

National Institute for Subatomic Physics

180

Université Paris Cité

149

Aix-Marseille Université

141

Laboratoire AstroParticule et Cosmologie

138

Parc Científic de la Universitat de València

137

Universitat Politècnica de València

127

Friedrich-Alexander-Universität Erlangen-Nürnberg

107

Universitat de València

101

Instituto de Física Corpuscular

100

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Papers

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Transcriptional Programs of Early Reproductive Stages in Arabidopsis

PLANT PHYSIOLOGY (2004)

Lars Hennig Wilhelm Gruissem Ueli Grossniklaus Claudia Köhler

Abstract The life cycle of flowering plants alternates between a diploid sporophytic and a haploid gametophytic generation. After fertilization of each the egg and central cells by one male gamete, the development of both fertilization products occurs coordinated with the maternally derived seed coat and carpel tissues forming the fruit. The reproduction program is likely to involve the concerted activity of many genes. To identify genes with specific functions during reproduction, we have analyzed the expression profile of more than 22,000 genes present on the Arabidopsis ATH1 microarray during three stages of flower and fruit development. We found 1,886 genes regulated during reproductive development and 1,043 genes that were specifically expressed during reproduction. When compared to cells from an Arabidopsis suspension culture, S-phase genes were underrepresented and G2 and M-phase genes were strongly enriched in the set of specific genes, indicating that important functions during reproduction are exerted in the G2 and M phases of the cell cycle. Many potential signaling components, such as receptor-like protein kinases, phosphatases, and transcription factors, were present in both groups of genes. Members of the YABBY, MADS box, and Myb transcription factor families were significantly overrepresented in the group of specific genes, revealing an important role of these families during reproduction. Furthermore, we found a significant enrichment of predicted secreted proteins smaller than 15 kD that could function directly as signaling molecules or as precursors for peptide hormones. Our study provides a basis for targeted reverse-genetic approaches aimed to identify key genes of reproductive development in plants.

MYB

Gamete

Sexual reproduction

10.1104/pp.104.043182

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Citations (135)

WinPep 2.11: novel software for PC-based analyses of amino acid sequences.

PubMed (2001)

Lars Hennig

WinPep 2.11 is the latest version of a user-friendly, versatile software for the analysis of amino acid sequences. WinPep was developed for Windows 95/98 and WindowsNT. Because of the standard windows techniques (copy & paste) and an intuitive user interface, no lengthy training is required to work with this program. Main features include calculation of molecular weight, isoelectric point, and molar absorption coefficients, as well as simulation of sequence specific cleavage. In addition to data analysis, data presentation is greatly assisted by the program, e.g., by producing helical wheel displays and hydropathy plots. The implemented functions of WinPep and it's usage are described in this publication.

Interface (matter)

Sequence (biology)

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Citations (15)

ArabidopsisRETINOBLASTOMA-RELATED Is Required for Stem Cell Maintenance, Cell Differentiation, and Lateral Organ Production

The Plant Cell (2010)

Lorenzo Borghi Ruben Gutzat Johannes Fütterer Yec’han Laizet Lars Hennig

Abstract Several genes involved in the regulation of postembryonic organ initiation and growth have been identified. However, it remains largely unclear how developmental cues connect to the cell cycle. RETINOBLASTOMA RELATED (RBR) is a plant homolog of the tumor suppressor Retinoblastoma (pRb), which is a key regulator of the cell cycle. Using inducible RNA interference (RNAi) against Arabidopsis thaliana RBR (RBRi), we reduced RBR expression levels at different stages of plant development. Conditional reduction or loss of RBR function disrupted cell division patterns, promoted context-dependent cell proliferation, and negatively influenced establishment of cell differentiation. Several lineages of toti- and pluripotent cells, including shoot apical meristem stem cells, meristemoid mother cells, and procambial cells, failed to produce appropriately differentiated cells. Meristem activity was altered, leading to a disruption of the CLAVATA-WUSCHEL feedback loop and inhibition of lateral organ formation. Release of RBR from RNAi downregulation restored meristem activity. Gene profiling analyses soon after RBRi induction revealed that a change in RBR homeostasis is perceived as a stress, even before genes regulated by RBR-E2F become deregulated. The results establish RBR as a key cell cycle regulator required for coordination of cell division, differentiation, and cell homeostasis.

Retinoblastoma protein

E2F

10.1105/tpc.110.074591

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Citations (165)

Additional file 3 of Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis

Figshare (2020)

Lejon E. M. Kralemann Shujing Liu Minerva S. Trejo‐Arellano Rafael Muñoz‐Viana Claudia Köhler

Additional file 3:Table S2. GO analysis of genes upregulated in ubp12, ubp13, and ubp12/13.

Deubiquitinating enzyme

10.6084/m9.figshare.12495119

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Citations (0)

Additional file 17 of Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis

Figshare (2020)

Lejon E. M. Kralemann Shujing Liu Minerva S. Trejo‐Arellano Rafael Muñoz‐Viana Claudia Köhler

Additional file 17: Table S16. Differential H3K27me3 in ubp12/13 vs wt.

Deubiquitinating enzyme

10.6084/m9.figshare.12495098

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Recombinant Phytochrome A in Yeast Differs by its Spectroscopic and Photochemical Properties from the Major phyA′ and is Close to the Minor phyA″: Evidence for Posttranslational Modification of the Pigment in Plants¶

Photochemistry and Photobiology (2001)

V.A. Sineshchekov Lars Hennig Tilman Lamparter Jon Hughes Wolfgang Gärtner

Previously, two pools of phytochrome A (phyA' and phyA") have been detected by in situ low-temperature fluorescence spectroscopy and photochemistry; it was suggested that they might differ in the nature of their posttranslational modification. In order to verify this possibility Arabidopsis and rice (Oryza) phyA were expressed in yeast and the pigments were assembled in vivo with phycocyanobilin (PCB) and phytochromobilin (P phi B). The resulting recombinant phytochromes in the red-light-absorbing form (Pr) were characterized in the yeast cell by (1) the fluorescence emission spectra; (2) the temperature dependence of Pr fluorescence intensity and activation energy of fluorescence decay; and (3) the extent of photoconversion of Pr into photoproduct lumi-R (gamma 1) or far-red-light absorbing form (Pfr) (gamma 2). Both Arabidopsis phyA/PCB and Oryza phyA/P phi B had low gamma 1 of ca 0.05, allowing their attribution to the Pr" phenomenological type of phytochrome comprising phyA", phyB and cryptogam phytochromes. The spectroscopic properties of Oryza phyA/P phi B were also very close to phyA". However, both investigated holoproteins differed from phyA", both with respect to the character of temperature dependence of the fluorescence yield and activation energy. Thus, recombinant Oryza phyA/P phi B is similar but not identical to phyA". The data demonstrate that the low-abundance-fraction plant phyA (phyA") comes from the same gene as the major (phyA') fraction. Because both endogenous phyA fractions differ from the phytochrome expressed in yeast, they appear to be posttranslationally modified and/or bound to partner proteins or cellular substructures. However, the character of the presumed chemical modification is different in phyA' and phyA" and its extent is more profound in the case of the former.

Phytochrome A

Phytochrome

Phycocyanobilin

10.1562/0031-8655(2001)073<0692:rpaiyd>2.0.co;2

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Citations (19)

Tissue-specific transposon-associated small RNAs in the gymnosperm tree, Norway spruce

BMC Genomics (2019)

Miyuki Nakamura Claudia Köhler Lars Hennig

Abstract Background Small RNAs (sRNAs) are regulatory molecules impacting on gene expression and transposon activity. MicroRNAs (miRNAs) are responsible for tissue-specific and environmentally-induced gene repression. Short interfering RNAs (siRNA) are constitutively involved in transposon silencing across different type of tissues. The male gametophyte in angiosperms has a unique set of sRNAs compared to vegetative tissues, including phased siRNAs from intergenic or genic regions, or epigenetically activated siRNAs. This is contrasted by a lack of knowledge about the sRNA profile of the male gametophyte of gymnosperms. Results Here, we isolated mature pollen from male cones of Norway spruce and investigated its sRNA profiles. While 21-nt sRNAs is the major size class of sRNAs in needles, in pollen 21-nt and 24-nt sRNAs are the most abundant size classes. Although the 24-nt sRNAs were exclusively derived from TEs in pollen, both 21-nt and 24-nt sRNAs were associated with TEs. We also investigated sRNAs from somatic embryonic callus, which has been reported to contain 24-nt sRNAs. Our data show that the 24-nt sRNA profiles are tissue-specific and differ between pollen and cell culture. Conclusion Our data reveal that gymnosperm pollen, like angiosperm pollen, has a unique sRNA profile, differing from vegetative leaf tissue. Thus, our results reveal that angiosperm and gymnosperm pollen produce new size classes not present in vegetative tissues; while in angiosperm pollen 21-nt sRNAs are generated, in the gymnosperm Norway spruce 24-nt sRNAs are generated. The tissue-specific production of distinct TE-derived sRNAs in angiosperms and gymnosperms provides insights into the diversification process of sRNAs in TE silencing pathways between the two groups of seed plants.

Gymnosperm

10.1186/s12864-019-6385-7

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Citations (15)

Acoustic positioning for deep sea neutrino telescopes with a system of piezo sensors integrated into glass spheres

Experimental Astronomy (2025)

A. Albert S. Alves M. André M. Ardid S. Ardid

Abstract Position calibration in the deep sea is typically done by means of acoustic multilateration using three or more acoustic emitters installed at known positions. Rather than using hydrophones as receivers that are exposed to the ambient pressure, the sound signals can be coupled to piezo ceramics glued to the inside of existing containers for electronics or measuring instruments of a deep sea infrastructure. The ANTARES neutrino telescope operated from 2006 until 2022 in the Mediterranean Sea at a depth exceeding 2000 m . It comprised nearly 900 glass spheres with 432 mm diameter and 15 mm thickness, equipped with photomultiplier tubes to detect Cherenkov light from tracks of charged elementary particles. In an experimental setup within ANTARES, piezo sensors have been glued to the inside of such – otherwise empty – glass spheres. These sensors recorded signals from acoustic emitters with frequencies from 46545 to 60235 Hz . Two waves propagating through the glass sphere are found as a result of the excitation by the waves in the water. These can be qualitatively associated with symmetric and asymmetric Lamb-like waves of zeroth order: a fast (early) one with $$\varvec{v_e \approx 5\,{\textbf {mm}}/\mu \text {s}}$$ v e ≈ 5 mm / μ s and a slow (late) one with $$\varvec{v_\ell \approx \,2\,{\textbf {mm}}/\mu \text {s}}$$ v ℓ ≈ 2 mm / μ s . Taking these findings into account improves the accuracy of the position calibration. The results can be transferred to the KM3NeT neutrino telescope, currently under construction at multiple sites in the Mediterranean Sea, for which the concept of piezo sensors glued to the inside of glass spheres has been adapted for monitoring the positions of the photomultiplier tubes.

10.1007/s10686-024-09971-7

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PRC2 Represses Hormone-Induced Somatic Embryogenesis in Vegetative Tissue of Arabidopsis thaliana

PLoS Genetics (2017)

Iva Mozgová Rafael Muñoz‐Viana Lars Hennig

Many plant cells can be reprogrammed into a pluripotent state that allows ectopic organ development. Inducing totipotent states to stimulate somatic embryo (SE) development is, however, challenging due to insufficient understanding of molecular barriers that prevent somatic cell dedifferentiation. Here we show that Polycomb repressive complex 2 (PRC2)-activity imposes a barrier to hormone-mediated transcriptional reprogramming towards somatic embryogenesis in vegetative tissue of Arabidopsis thaliana. We identify factors that enable SE development in PRC2-depleted shoot and root tissue and demonstrate that the establishment of embryogenic potential is marked by ectopic co-activation of crucial developmental regulators that specify shoot, root and embryo identity. Using inducible activation of PRC2 in PRC2-depleted cells, we demonstrate that transient reduction of PRC2 activity is sufficient for SE formation. We suggest that modulation of PRC2 activity in plant vegetative tissue combined with targeted activation of developmental pathways will open possibilities for novel approaches to cell reprogramming.

Reprogramming

PRC2

Totipotent

Ectopic expression

10.1371/journal.pgen.1006562

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Citations (100)

Functional Genomic Analysis of CAF-1 Mutants in Arabidopsis thaliana

Journal of Biological Chemistry (2006)

Nicole Schönrock Vivien Exner Aline V. Probst Wilhelm Gruissem Lars Hennig

10.1074/jbc.m513426200

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Citations (125)