Amedeo Manfrin

Istituto Zooprofilattico Sperimentale delle Venezie

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Eleonora Fiocchi

Istituto Zooprofilattico Sperimentale delle Venezie

Pierluigi Carbonara

Tecnologia e Ricerca Italiana (Italy)

Giuseppe Lembo

University of Malta

Walter Zupa

Fondazione Italiana Fegato

Sébastien Alfonso

Ecology and Conservation Science for Sustainable Seas

Anna Toffan

Istituto Zooprofilattico Sperimentale delle Venezie

Tobia Pretto

Istituto Zooprofilattico Sperimentale delle Venezie

Giuseppe Arcangeli

Istituto Zooprofilattico Sperimentale delle Venezie

María Teresa Spedicato

Stazione Sperimentale per l'Industria delle Pelli e delle Materie Concianti

Manuela Dalla Pozza

Istituto Zooprofilattico Sperimentale delle Venezie

Cooperative Institutions

Istituto Zooprofilattico Sperimentale delle Venezie

University of Padua

University of Bologna

Royal College of Surgeons in Ireland

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"

University of Milan

Technical University of Denmark

Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche

Université Paris-Saclay

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

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First isolation of koi herpes virus (KHV) in Italy from imported koi (Cyprinus carpio koi)

Zenodo (CERN European Organization for Nuclear Research) (2013)

Tobia Pretto Amedeo Manfrin Chiara Ceolin Manuela Dalla Pozza Zelco Stefano

Isolation

Virus isolation

10.5281/zenodo.7767544

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Citations (8)

Ultrastructural and molecular characterization of Vairimorpha austropotamobii sp. nov. (Microsporidia: Burenellidae) and Thelohania contejeani (Microsporidia: Thelohaniidae), two parasites of the white-clawed crayfish, Austropotamobius pallipes complex (Decapoda: Astacidae)

Journal of Invertebrate Pathology (2017)

Tobia Pretto Francesco Montesi Daniela Ghia Valeria Berton Miriam Abbadi

Polar filament

10.1016/j.jip.2017.11.002

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Citations (22)

Workshop: How outputs from EU projects can upgrade health management in the Mediterranean aquaculture

Bulletin of the European Association of Fish Pathologists (2022)

Snježana Zrnčić Francesc Padrós S Tavornapanich Niels Lorenzen Donatella Volpatti

Aquaculture of European sea bass ( Dicentrarchus labrax ) and gilthead sea bream ( Sparus aurata ) is an essential activity in the Mediterranean basin. Several EU Horizon 2020 and regional projects are focusing on the improvement of their farming performance. This workshop aimed at displaying, sharing and discussing projects’ achievements among interested colleagues engaged to work with fish diseases. The most innovative research outputs aiming to improve the European mariculture were presented during this workshop, including updates from EU Horizon 2020 projects MedAID, PerformFISH, ParaFishControl, FutureEUAqua and the Italy-Croatia Interreg project AdriAquaNet.

Mariculture

Dicentrarchus

Sea bass

Mediterranean Basin

Black Sea

10.48045/001c.35854

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Redfin perch juveniles, Perca fluviatilis L., are resistant to European viral haemorrhagic septicaemia virus and infectious haematopoietic necrosis virus delivered via immersion in experimental condition

Journal of Fish Diseases (2014)

Francesco Pascoli F. Borghesan Amedeo Manfrin Francesco Quaglio Francesca Gatti

10.1111/jfd.12272

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Citations (1)

ASSESSING PHYSIOLOGICAL EFFECTS OF FEEDING AN INNOVATIVE DIET IN FARMED EUROPEAN SEA BASS (Dicentrarchus labrax)

Zenodo (CERN European Organization for Nuclear Research) (2022)

Pierluigi Carbonara Sébastien Alfonso Eleonora Fiocchi Amedeo Manfrin Annalisa Stefani

Dicentrarchus

Sea bass

10.5281/zenodo.8265189

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Occurrence of nodular gill disease in farmed brown trout (Salmo trutta L.)

Journal of Fish Diseases (2019)

Alberto Perolo Andrea Gustinelli Marialetizia Fioravanti Amedeo Manfrin Manuela Dalla Pozza

The authors Alberto Perolo, Andrea Gustinelli, Maria Letizia Fioravanti, Amedeo Manfrin, Manuela Dalla Pozza, Fernando Lunelli, Adelchi Accini and Francesco Quaglio certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership or other equity interest; and expert testimony or patent-licensing arrangements) or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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Equity

Brown trout

10.1111/jfd.13027

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Growth Performance, Immune Characteristics, and Health and Welfare of Gilthead Seabream (Sparus aurata) Fed a Tailor‐Made Environmentally Sustainable Diet Formulated Using Novel Ingredients

Aquaculture Research (2024)

Sébastien Alfonso Lola Toomey Eleonora Fiocchi Amedeo Manfrin Marilena Boscarato

The use of fish meal/oil in carnivorous fish feeds remains a concern for the environmental sustainability of aquaculture. In this study, we investigated the impact of an innovative diet designed to be cost‐effective and environmentally sustainable (i.e., 60% replacement of fish meal by a blend of plant, yeast [ Saccharomyces cerevisiae ], and krill meal feed ingredients) on the growth, health, and welfare of gilthead seabream ( Sparus aurata ). Over a 135‐day experiment, fish were fed either the innovative or a commercial diet (control), and various parameters were evaluated, namely growth performance, levels of physiological blood parameters related to stress, immunity, health, and welfare, as well as swimming activity, serving as a proxy for energy expenditure. Results revealed that the innovative diet enhanced growth compared to fish fed the control diet. Hematological and biochemical indicators did not highlight any impaired welfare condition in fish fed innovative diet while higher levels of Immunoglobulin M were measured in plasma of fish fed innovative diet, potentially suggesting enhancement of humoral immunity. However, accelerometer tags data revealed that fish fed the innovative diet exhibited higher overall swimming activity, suggesting higher energy expenditure, which was consistent with greater prealbumin levels measured in the plasma. In conclusion, the higher energy metabolism in fish fed the innovative diet might be compensated by the diet’s content, which may boost humoral immunity and hence help the fish develop a better adaptation to rearing environment, including its viral and bacterial load, ensuring overall better growth. Longer term investigations, including measurements of additional parameters, are required to validate these promising preliminary outcomes.

10.1155/2024/8234882

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Genomic Diversity and Evolution of the Fish Pathogen Flavobacterium psychrophilum

Frontiers in Microbiology (2018)

Éric Duchaud Tatiana Rochat Christophe Habib Paul Barbier Valentin Loux

Flavobacterium psychrophilum, the etiological agent of rainbow trout fry syndrome and bacterial cold-water disease in salmonid fish, is currently one of the main bacterial pathogens hampering the productivity of salmonid farming worldwide. In this study, the genomic diversity of the F. psychrophilum species is analyzed using a set of 41 genomes, including 30 newly sequenced isolates. These were selected on the basis of available MLST data with the two-fold objective of maximizing the coverage of the species diversity and of allowing a focus on the main clonal complex (CC-ST10) infecting farmed rainbow trout (Oncorhynchus mykiss) worldwide. The results reveal a bacterial species harboring a limited genomic diversity both in terms of nucleotide diversity, with ~0.3% nucleotide divergence inside CDSs in pairwise genome comparisons, and in terms of gene repertoire, with the core genome accounting for ~80% of the genes in each genome. The pan-genome seems nevertheless "open" according to the scaling exponent of a power-law fitted on the rate of new gene discovery when genomes are added one-by-one. Recombination is a key component of the evolutionary process of the species as seen in the high level of apparent homoplasy in the core genome. Using a Hidden Markov Model to delineate recombination tracts in pairs of closely related genomes, the average recombination tract length was estimated to ~4.0 Kbp and the typical ratio of the contributions of recombination and mutations to nucleotide-level differentiation (r/m) was estimated to ~13. Within CC-ST10, evolutionary distances computed on non-recombined regions and comparisons between 22 isolates sampled up to 27 years apart suggest a most recent common ancestor in the second half of the nineteenth century in North America with subsequent diversification and transmission of this clonal complex coinciding with the worldwide expansion of rainbow trout farming. With the goal to promote the development of tools for the genetic manipulation of F. psychrophilum, a particular attention was also paid to plasmids. Their extraction and sequencing to completion revealed plasmid diversity that remained hidden to classical plasmid profiling due to size similarities.

Nucleotide diversity

10.3389/fmicb.2018.00138

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Citations (55)

Carp edema virus‐related mortality in wild adult common carp (cyprinus carpio) in Italy

Journal of Fish Diseases (2021)

Andrea Marsella Tobia Pretto Miriam Abbadi Rosita Quartesan Luana Cortinovis

Abstract Mortality in wild fish populations represents a challenging issue for public fish health inspectors. When a single fish species is involved, an infective aetiology is frequently suspected, with focus on viral notifiable diseases. However, other viral agents not subjected to regulation and causing mortality in common carp have been reported such as carp edema virus (CEV). In mid‐June 2020, a severe common carp mortality was observed in an artificial lake in north‐east of Italy. Sleepy fish were noted some days before the beginning of the mortality itself, which lasted several days and involved over 340 adult specimens. During the outbreak, water temperature was around 15°C, water quality was normal, and no adverse meteorological events were reported in the area. Four specimens, which showed severe cutaneous hyperaemia and increased mucus production on skin and gills, were tested by bacteriological methods and virological analysis targeting the main carp pathogens. Molecular analysis performed on gills, kidney and brains from all the fish analysed resulted positive for CEV, which, based on anamnestic information and laboratory findings, was considered the responsible for the mortality event herein described.

Common carp

Fish mortality

10.1111/jfd.13353

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Citations (11)

ABSTRACTS

Pharmacoepidemiology and Drug Safety (2018)

C Bains Catherine J. Byrne Amy Campbell M Cashin-Cox R. Michael Cassidy

Background: Medication waste is a significant loss of potential funding to the NHS.Figures from 2010 showed that around £300 million of medication is wasted annually and a sixth of this is accountable to care homes. 1 Aim: We aimed to audit the extent of this medication waste in NHS Sunderland CCG and implement pharmacy technician support in order to reduce this.A re-audit was then carried out to determine the impact of this support.Methods: An audit form was designed and tested.Each care home was visited during a baseline audit period.Data from the audit were then used to provide support to the care home.Waste was categorized into avoidable and unavoidable waste.Identified avoidable waste was communicated to the care homes, changes to medicines management practices were suggested such as amendments to ordering procedures to avoid unnecessary prescription generation.Twenty care homes were included in the waste reduction programme.Re-audits following this advice were then carried out after a 5-month period.Results: Initial audits found that there was an average of £202.63 of medication wasted per care home for each 28-day cycle of medication.Following support from a pharmacy technician, this reduced to £103.33, saving £25,818 of medication per year to the CCG.

10.1002/pds.4439

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Citations (2)