Sergey V. Chepanov

Research Institute of Obstetrics and Gynecology named after D.O. Ott

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SА

S. А. Selkov

Research Institute of Obstetrics and Gynecology named after D.O. Ott

ДИ

Д. И. Соколов

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Roman V. Kapustin

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Olga N. Arzhanova

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Igor Yu. Kogan

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Ekaterina V. Kopteeva

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Olesya N. Bespalova

Research Institute of Obstetrics and Gynecology named after D.O. Ott

МС

М. С. Зайнулина

First Pavlov State Medical University of St. Petersburg

НН

Н. Н. Петрищев

First Pavlov State Medical University of St. Petersburg

M. I. Krivonos

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Cooperative Institutions

Research Institute of Obstetrics and Gynecology named after D.O. Ott

First Pavlov State Medical University of St. Petersburg

St Petersburg University

Russian Academy of Sciences

Ministry of Health of the Russian Federation

Federal Almazov North-West Medical Research Centre

Academy of Medical Sciences

Institute of Experimental Medicine

Saint Petersburg State Pediatric Medical University

City Hospital No. 40

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Use of plasmapheresis and intravenous immunoglobulins in pregnant women with antiphospholipid syndrome

Medical Immunology (Russia) (2025)

E. S. Orlova Sergey V. Chepanov М. С. Зайнулина S. А. Selkov

Our objective was to demonstrate the efficiency of therapy for antiphospholipid syndrome (APS) in pregnant women using plasmapheresis and intravenous immunoglobulins. 92 women with diagnosed APS and 47 APS-free women with physiological pregnancy were under study. APS was diagnosed in accordance with Sydney Consensus Workshop (2006). Patients with APS were divided into groups depending on the method of treatment used: conventional therapy, plasmapheresis in addition to standard therapy, intravenous immunoglobulins (IVIG) in addition to standard therapy, and complex treatment, i.e., plasmapheresis and IVIG added to standard therapy. The levels of antiphospholipid antibodies, hemostasis parameters, P-selectin were measured in blood serum and plasma before and after treatment. Higher frequency of favorable pregnancy outcomes was shown in the group of patients with APS who were treated with combined therapy, i.e. in 96.3% of cases (term birth). Frequency of prematurity and fetal hypotrophy was significantly lower in the group of patients with APS treated with combined therapy. The most significant decrease in the level of antiphospholipid antibodies was observed in the group treated by plasmapheresis, IVIG and conventional therapy. Expression of P-selectin in the women with normal pregnancy without antiphospholipid antibodies, was significantly lower compared to pregnant women with APS. Usage of integrated approach using plasmapheresis, intravenous immunoglobulins and standard therapy is the most effective treatment for APS-related miscarriage. Implication of this strategy has reduced the incidence of obstetric complications and improved pregnancy outcomes due to increased frequency of term births, as well as lowest indices of pathology in the newborns. Analysis of P-selectin levels before and after treatment enables determination of the platelet activation levels, efficiency of therapeutic approaches, as well as medical drug correction of infavorable platelet hemostasis during therapy.

Plasmapheresis

Intravenous Immunoglobulins

10.15789/1563-0625-uop-2893

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Autosensitization to human chorionic gonadotropin: etiology, role in reproductive loss, and approaches to therapy

Journal of obstetrics and women s diseases (2023)

Daria V. Tkhai Sergey V. Chepanov Olga N. Arzhanova Olesya N. Bespalova S. А. Selkov

The important role of human chorionic gonadotropin in pregnancy suggests the occurrence of reproductive disorders due to the circulation of specific antibodies, especially in early pregnancy, which can lead to a cascade of pathological reactions such as implantation failure, placentation, trophoblast invasion, and ultimately fetal rejection. The lack of knowledge and the complexity of diagnosing and treating this autoimmune pathology in reproductive medicine and obstetrics negatively affects both the outcomes of each individual pregnancy and the demographic situation as a whole. This review article highlights the main ideas about the causes of human chorionic gonadotropin antibody occurrence, the pathological mechanisms, the reproductive and obstetric complications associated with human chorionic gonadotropin autoantibodies, diagnostic methods, and therapeutic approaches.

Human chorionic gonadotropin

Placentation

Etiology

Gonadotropin

Trophoblast

10.17816/jowd114793

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Recombinant IL-2: clinical efficacy and pathogenetic rationale for use in external genital endometriosis

Russian Journal of Human Reproduction (2021)

Maria I. Yarmolinskaya E.I. Durneva Sergey V. Chepanov Д. И. Соколов S. А. Selkov

Sex organ

Clinical efficacy

10.17116/repro20212702141

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Extended combined screening for preeclampsia prediction in women with diabetes mellitus

Journal of obstetrics and women s diseases (2023)

Roman V. Kapustin Elizaveta M. Tcybuk Ekaterina V. Kopteeva Elena N. Alekseenkova Sergey V. Chepanov

BACKGROUND:Early screening for preeclampsia has shown high efficiency for low-risk groups, however, the presence of systemic vascular disease in patients with diabetes mellitus complicates their use and requires the development of additional approaches to predicting preeclampsia in this group of patients. AIM:The aim of this study was to evaluate the effectiveness of early prediction of preeclampsia with extended combined screening in patients with pregestational types of diabetes mellitus. MATERIALS AND METHODS:This study included 75 pregnant women: 40 patients with type 1 diabetes mellitus, and 35 patients with type 2 diabetes mellitus. To determine the risk of further preeclampsia development, we evaluated biochemical, biophysical and anamnestic factors, along with the serum levels of placental growth factor, soluble fms-like tyrosine kinase 1, and soluble endoglin at 11+0to 13+6gestational weeks. The main outcome assessed was the development of preeclampsia. RESULTS:In patients with further development of preeclampsia (35% in type 1 and 40 % in type 2 diabetic women), we observed higher soluble fms-like tyrosine kinase 1 / placental growth factor ratios, as well as elevated serum soluble endoglin (type 1 diabetes mellitus) and soluble fms-like tyrosine kinase 1 (type 2 diabetes mellitus) levels. Isolated determination of placental growth factor showed no significant differences in the levels of this angiogenic factor in preeclampsia. A multivariate predictive model for preeclampsia demonstrated high prognostic parameters: for patients with type 1 diabetes mellitus, area under the curve was 0.96 (95% confidence interval 0.931.00), with positive predictive value 76.7% and negative predictive value 90.1%; for patients with type 2 diabetes mellitus, area under the curve was 0.94 (95% confidence interval 0.861.00), with positive predictive value 88.5% and negative predictive value 97.1%. CONCLUSIONS:Specific biochemical and biophysical markers of preeclampsia combined with maternal risk factors and assessment of carbohydrate metabolism can increase the predictive value of early screening studies for preeclampsia in women with pregestational diabetes mellitus.

10.17816/jowd321321

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Microvesicles produced by monocytes affect the phenotype and functions of endothelial cells

AIMS Allergy and Immunology (2021)

Д. И. Соколов А. Р. Козырева K. L. Markova V. A. Mikhailova А. В. Кореневский

Monocytes\macrophages regulate angiogenesis via cytokine production and contact interactions with endothelial cells (ECs). The biological effects of macrophage-derived microvesicles (MVs) are studied using cell lines, such as monocytic leukemia THP-1 cell line. The effect of MVs produced by THP-1 cells on EC phenotype and functions remain understudied. In this research, we studied the effect of MVs produced by THP-1 cells on the phenotype, proliferation, migration, and vascular formation of EA.Hy926 ECs. MVs produced by THP-1 cells express CD54, CD18, CD11a, CD11b, CD29, CD120a, CD120b, VEGFR1, VEGFR2, CD105, CD119, TGFR2 on the surface and contain ERK1/2, pERK1/2 Akt, FGF10, endothelin-2. The transfer of an intracellular protein labeled with a fluorescent dye from MVs produced by THP-1 cells to EA.Hy926 ECs was established. It was found that MVs derived from THP-1 cells inhibit EC proliferation. In high concentrations, MVs reduce EC migration, increase the length but decrease the number of vessels formed by ECs, promoting the development of non-branching angiogenesis. On the contrary, in low concentrations, MVs increase EC migration, reduce the length, and increase the number of vessels formed by ECs, promoting the development of branching angiogenesis. Thus, the fundamental possibility of the influence of MVs produced by THP-1 cells on the processes of angiogenesis has been established. Proteins found in the MVs composition may be responsible for the observed effects of MVs on ECs.

THP1 cell line

10.3934/allergy.2021011

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Effects of Nothrombel on Thrombin-Induced Formation of Thrombocyte-Leukocyte Complexes

Neuroscience and Behavioral Physiology (2018)

О. С. Веселкина Н. Н. Петрищев Л. В. Васина M. E. Borovitov A. V. Selyutin

Mechanism of Action

10.1007/s11055-018-0636-y

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Antiphospholipid syndrome in miscarriage

Journal of obstetrics and women s diseases (2015)

Tat’yana Nikolaevna Shlyakhtenko Ekaterina Anatol’yevna Alyab’yeva Olga N. Arzhanova S. А. Selkov Tamara Aleksandrovna Pluzhnicova

Purpose: the frequency of antiphospholipid in patients with reproductive failures was defined. The algorithm of the treatment of these patients was presented. Materials and methods: the study included 2150 patients: 1650 with a history of the repeated pregnancy losses; 450 with sterility and 50 healphy women. Results: the high levels of AFA were founded in 24 % of patients with spontaneous abortions and in 20 % of patients with sterility. Conclusion: Pregravid preparation and permanent monitoring the state of patients reduce frequency of unfavorable ends of pregnancy effectively.

Recurrent miscarriage

10.17816/jowd64569-76

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Citations (4)

Maternal serum nitrotyrosine, 8-isoprostane and total antioxidant capacity levels in pre-gestational or gestational diabetes mellitus

Gynecological Endocrinology (2020)

Roman V. Kapustin Sergey V. Chepanov Ekaterina V. Kopteeva Olga N. Arzhanova

Objective To evaluate serum concentration of 8-isoprostane, nitrotyrosine (NT), and total antioxidant capacity (TAC) in pregnant women with diabetes mellitus (DM) considering preconception planning and method of diabetes correction in 11–14 and 30–34 weeks. Materials and methods The study included 130 women: T1DM (n = 40), T2DM (n = 35), gestational diabetes (GDM, n = 40) and the control group (n = 15). The serum concentrations of NT, 8-isoprostane, and TAC were measured by ELISA methods.Results Elevated 8-isoprostane levels were observed in all patients with DM, but this biomarker's maximum values have been seen in T1DM and T2DM on insulin groups. A similar tendency was observed for the concentration of NT in both the 1st and 3rd trimesters. TAC levels showed a statistically relevant decrease in all DM groups compared to the control. The correlation analysis showed a direct correlation between HbA1c and serum 8-isoprostane levels in the 1st (r = .27) and 3rd (r = .3) pregnancy trimesters as well as inverse correlation with TAC level (r = −.48). Direct (NT, 8-isoprostane) and inverse correlations (TAC) were fixated for this biomarker concentration and preeclampsia rates.Conclusion DM in pregnancy is related to oxidative stress activation, which might lead to the development of adverse perinatal outcomes.

Nitrotyrosine

Isoprostanes

10.1080/09513590.2020.1816727

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IMMUNOREGULATORY EFFECTS OF INTRAVENOUS IMMUNOGLOBULINS

Medical Immunology (Russia) (2014)

S. А. Selkov Д. И. Соколов Sergey V. Chepanov

Treatment with intravenous immunoglobulins (IVIG) is widely used in modern clinical practice inorder to cure different clinical disorders, including obstetric conditions. Currently, IVIGs have become drugs of choice for treatment of anti-phospholipid syndrome in pregnant women, like as in cases of intrauterine cytomegalovirus infection.

Intravenous Immunoglobulins

Cytomegalovirus

Clinical Practice

Intravenous Use

10.15789/1563-0625-2013-1-5-12

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An impact of the combined drug containing antimicrobial, antiprotozoal, antifungal and glucocorticoid agents on local immunity in women with bacterial vaginosis and non-specific vaginitis

Russian Journal of Infection and Immunity (2021)

O. M. Bokach M. S. Murasheva M. S. Selkova Kseniia V. Storozheva Sergey V. Chepanov

Inflammatory diseases of the vagina related to imbalanced microflora composition represent one of the most common gynecological diseases primarily dealt with by obstetrician/gynecologists. Such diseases markedly worsen female patient quality of life as well as affect female reproductive system resulting both in altered fertility and adverse pregnancy outcomes. By now, multiple therapeutic and preventive protocols have been proposed to manage such diseases, but insufficient therapeutic efficacy and frequent relapses require further investigations. The aim of the study was to evaluate an effect of the drug Elgyna (Wertex, Russia) consisting of antibacterial, antifungal and glucocorticoid agents on local immunity in females with bacterial vaginosis and nonspecific vaginitis. For this, there were examined and treated 24 females enrolled to the study, who underwent gynecological examination (speculum and bimanual vaginal examination), cervical epithelium microscopy, and calculating karyopicnotic index. Special attention was paid to quantifying level of cytokines IL-6, IL-10, TNFα in vaginal smears before and after therapy with the drug Elgyna. It was found that by the end of the therapy course vaginally administered Elgyna resulted in significantly ameliorated clinical symptoms and normalized microscopic parameters of vaginal discharge. Moreover, this drug consisting of antibacterial, antifungal and glucocorticoid agents did not suppress vaginal epithelium proliferation, but lowered production of pro-inflammatory cytokine TNFα in vaginal smears and shifted toward anti-inflammatory cytokine production. This evidence pathogenetically justifies administration of the drug Elgyna in females with bacterial vaginosis and nonspecific vaginitis as an anti-inflammatory medicine. Efficacy and safety of the two-step therapeutic approach consisting of antibacterial therapy followed by vaginal microbiota recovery for treating inflammatory diseases of vaginal mucosa were confirmed in multiple randomized clinical trials. We envision that such therapeutic concept might be added with a third component implying correction of local vaginal immunity aimed at normalizing the balance between pro- and anti-inflammatory cytokines.

Vaginal disease

10.15789/2220-7619-aio-1462

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