Jiechang Tang

Chongqing University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Xiaowei Chen

Army Medical University

Yan Wang

Guangdong Medical College

Longhui Li

Ningbo Kangning Hospital

Xingyi Li

Zhengzhou University of Light Industry

Rou Xue

China Medical University

Min Li

Institute of Optics and Electronics, Chinese Academy of Sciences

Janelle M.P. Pakan

Otto-von-Guericke University Magdeburg

Hongbo Jia

Chinese Academy of Sciences

Cooperative Institutions

Chinese Academy of Sciences

Army Medical University

Suzhou Institute of Biomedical Engineering and Technology

Leibniz Institute for Neurobiology

Center for Behavioral Brain Sciences

Chongqing University

Otto-von-Guericke University Magdeburg

Center for Excellence in Brain Science and Intelligence Technology

Guangzhou Medical University

Guangzhou First People's Hospital

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Optical Fiber-Based Recording of Climbing Fiber Ca2+ Signals in Freely Behaving Mice

Biology (2022)

Jiechang Tang Rou Xue Yan Wang Min Li Hongbo Jia

The olivocerebellar circuitry is important to convey both motor and non-motor information from the inferior olive (IO) to the cerebellar cortex. Several methods are currently established to observe the dynamics of the olivocerebellar circuitry, largely by recording the complex spike activity of cerebellar Purkinje cells; however, these techniques can be technically challenging to apply in vivo and are not always possible in freely behaving animals. Here, we developed a method for the direct, accessible, and robust recording of climbing fiber (CF) Ca2+ signals based on optical fiber photometry. We first verified the IO stereotactic coordinates and the organization of contralateral CF projections using tracing techniques and then injected Ca2+ indicators optimized for axonal labeling, followed by optical fiber-based recordings. We demonstrated this method by recording CF Ca2+ signals in lobule IV/V of the cerebellar vermis, comparing the resulting signals in freely moving mice. We found various movement-evoked CF Ca2+ signals, but the onset of exploratory-like behaviors, including rearing and tiptoe standing, was highly synchronous with recorded CF activity. Thus, we have successfully established a robust and accessible method to record the CF Ca2+ signals in freely behaving mice, which will extend the toolbox for studying cerebellar function and related disorders.

Climbing fiber

Parallel fiber

Climbing

Toolbox

Tracing

Cerebellar hemisphere

10.3390/biology11060907

Cite

Citations (2)