Junjie Yu

Jiangsu Academy of Agricultural Sciences

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Yongfeng Liu

Zhejiang University

Xiayan Pan

Institute of Plant Protection

Tianqiao Song

Jiangsu Academy of Agricultural Sciences

Ning Yang

Jiangsu University

Rongsheng Zhang

Jiangsu Academy of Agricultural Sciences

Huijuan Cao

Beijing University of Chinese Medicine

Mina Yu

Institute of Plant Protection

Zhongqiang Qi

Jiangsu Academy of Agricultural Sciences

Dong Liang

Nanjing Maternity and Child Health Care Hospital

Mingguo Zhou

Nanjing Agricultural University

Cooperative Institutions

Jiangsu Academy of Agricultural Sciences

Institute of Plant Protection

Nanjing Agricultural University

Jiangsu University

Zhejiang University

Chinese Academy of Sciences

Agriculture and Agri-Food Canada

Ottawa Research and Development Centre

Peking University

University of Chinese Academy of Sciences

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Changes of Magnaporthe oryzae population in Jiangsu Province from 2001 to 2010.

Jiangsu nongye xuebao (2010)

Yongfeng Liu Zhiyi Chen Youzhou Liu Luo ChuPing Nie YaFeng

818 monosporous strains of Magnaporthe oryzae were isolated from 1 269 rice blast disease samples in five major rice planting areas in 2001-2010,which were identified by seven Chinese rice cultivars and classified into 7 groups and 40 races.The results revealed that ZG1 and ZB groups were dominant races in Jiangsu Province.ZG1 race exhibited the highest appearance frequency(58.3%) in 2001-2010 and ZB group was the second with 16.9% appearance frequency.The distribution of different M.grisea races varied in five major rice planting areas of Jiangsu Province.The race distribution was relatively simple,just 7 groups and 18 races in Xuzhou and Yancheng areas.However,in the other areas,the distribution was complicated,with 7 groups and 22 races in Nanjing,7 groups and 23 races in Suzhou,7 groups and 21 races in Nantong.In Suzhou,there was as more as had 11 races belonging to ZB group.The dominant race ZG1 kept high appearance frequency,with the highest 73% in 2006 and the lowest 41.4% in 2009.ZB groups reached the highest appearance frequency(31.4%) in 2009.The results indicated that rice cultivars exhibited high sensitivity and affinity to rice M.grisea races in Jiangsu Province.

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Low Temperature Storage and in vitro Pollen Germination of Selected Spring Wheat Accessions

Journal of Agricultural Science (2017)

Bahram Baninasab Mirko Tabori Junjie Yu Yuxue Zhang Xuelian Wang

The study was conducted to evaluate in vitro pollen germination of 50 spring wheat (Triticum aestivum L.) accessions at three storage temperatures (23 °C, 5 °C, and -20 °C). Germination of the mature pollen was measured right after harvest (0 time), and 24, 48, and 72 h after storage (HAS). Differences in fresh pollen germination between accessions were significant and ranged from 21.15% to 1.09%. Pollen germination was the lowest at 24 h at room temperature. The pollens of 24 wheat accessions (AAC Scotia, AW636, AW725, AW780, AW804, AW822, AW823, Brookfield, BRS Pardela, ECSW05, ECSW38, ECSW48, ECSW49, ECSW69, Hoffman, Kleos, Major, Nass, 12NQW-237, 12NQW-413, 12NQW-414, 12NQW-436, 12NQW-754, and 12BW0374) were viable after 24 h when they were stored at 5 °C. Sub-zero temperature did not prolong pollen viability.

10.5539/jas.v9n9p1

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Mutations in a β-Tubulin Confer Resistance of Gibberella zeae to Benzimidazole Fungicides

Phytopathology (2009)

Changjun Chen Junjie Yu Chaowei Bi Yannan Zhang Jianqiang Xu

ABSTRACT Wheat head blight caused by Gibberella zeae (anamorph: Fusarium graminearum) is a threat to food safety in China because of mycotoxin contamination of the harvested grain, the frequent occurrence of the disease, and the failure of chemical control in some areas due to benzimidazole resistance in the pathogen population. The molecular resistance mechanism, however, of G. zeae to benzimidazole fungicides (especially carbendazim; active ingredient: methyl benzimidazol-2-yl carbamate [MBC]) is poorly understood. DNA sequences of a beta-tubulin gene (beta(2)tub) (GenBank access number FG06611.1) in G. zeae were analyzed. Mutations in beta(2)tub in moderately resistant strains (MBC(MR)) included TTT (Phe)-->TAT (Tyr) at codon 167 or TTC (Phe)-->TAC (Tyr) at codon 200. A highly resistant strain (MBC(HR)) had two point mutations, one at codon 73, CAG (Gln)-->CGG (Arg), and the other at codon 198, GAG (Glu)-->CTG (Leu). To confirm that mutations in the beta(2)tub confer resistance to benzimidazole fungicides, the entire beta(2)tub locus was deleted from MBC(MR) and MBC(HR) strains of G. zeae. The resulting Deltabeta(2)tub mutants from both MBC(MR) and MBC(HR) strains grew normally on MBC-free potato dextrose agar medium and were supersensitive to MBC. Complementation of the Deltabeta(2)tub mutants by transformation with a copy of the intact beta(2)tub locus from their parent strains exhibited less resistance than the original strains, and complementation of the Deltabeta(2)tub mutants by transformation with a copy of the intact beta(2)tub locus from sensitive strains restored MBC sensitivity. The results indicated that the mutations in the beta(2)tub gene conferred resistance of G. zeae to benzimidazole fungicides and this gene can be used as a genetic marker in G. zeae.

Gibberella zeae

10.1094/phyto-99-12-1403

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A rapid rice blast detection and identification method based on crop disease spores' diffraction fingerprint texture

Journal of the Science of Food and Agriculture (2020)

Ning Yang Junjie Yu Aiying Wang Jian Tang Rongbiao Zhang

Rice blast fungus is a worldwide disease, and it is one of the most serious rice diseases in the north and south rice fields in China. The initial symptoms of rice blast are not obvious, and the speed of transmission is fast. Manual identification is time-consuming and laborious. At present, it is a great challenge to realize rapid and accurate early identification of rice blast.In this paper, an identification method based on crop disease spores' diffraction fingerprint texture for rice blast was studied; this method utilizes the light field and texture features of diffraction images. To verify the reliability of the model that we proposed, we selected two methods of manual identification and machine recognition to compare and detect rice blast spores. The experimental results show that the identification of light diffraction characteristics is not only higher than the traditional manual recognition by microscope (increased by more than 0.3%), but also faster after neural network training (increased by more than 90%). The diffraction recognition method used in this study, based on crop disease spores' diffraction fingerprint texture, can be completed in a few seconds, and its test accuracy is 97.18%.The proposed method, a rapid rice blast detection and identification method based on crop disease spores' diffraction fingerprint texture, has certain advantages compared with the existing manual identification by microscope. This method can be applied to the recognition of rice blast in agricultural research. © 2020 Society of Chemical Industry.

Texture (cosmology)

Identification

Plant disease

Blast disease

10.1002/jsfa.10383

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UvSMEK1, a Suppressor of MEK Null, Regulates Pathogenicity, Conidiation and Conidial Germination in Rice False Smut Fungus Ustilaginoidea virens

Rice Science (2021)

Junjie Yu Mina Yu Tianqiao Song Huijuan Cao Mingli Yong

Rice false smut, which is caused by Ustilaginoidea virens, is an emerging disease of rice spikelets in rice-growing areas worldwide. However, the infection mechanism of U. virens on rice spikelets is still unclear. Here, we characterized a suppressor of mitogen-activated protein kinase kinase or ERK kinase (MEK) null (UvSMEK1) in U. virens that is conserved among filamentous fungi. Compared with wild type U. virens strain P-1, UvSMEK1 deletion mutants were defective in pathogenicity and conidial germination. In addition, conidiation of UvSMEK1 deletion mutants was significantly reduced on yeast extract tryptone (YT) plates, but increased in YT broth compared with the wild type. Compared with UvSMEK1 expression level during the vegetative mycelia and conidiation stages, UvSMEK1 dramatically increased during infection of rice florets. Surprisingly, the UvSMEK1 deletion mutants exhibited higher tolerance to H2O2 and NaCl. In summary, presented evidence suggested that UvSMEK1 positively regulated pathogenicity, conidial germination and conidiation in YT broth, and negatively regulated conidiation on YT medium and tolerance to oxidative and osmotic stresses. The results enhance our understanding of the regulatory mechanism of pathogenicity of U. virens, and present a potential molecular target for blocking rice infection by U. virens.

Conidiation

Appressorium

Wild type

10.1016/j.rsci.2021.07.006

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Transfer of the β-tubulin gene of Botrytis cinerea with resistance to carbendazim into Fusarium graminearum

Pest Management Science (2010)

Shengming Liu Yu Chen Junjie Yu Changjun Chen Jianxin Wang

BACKGROUND: Resistance to carbendazim and other benzimidazole fungicides in Botrytis cinerea (Pers. ex Fr.) and most other fungi is usually conferred by mutation(s) in a single chromosomal β-tubulin gene, often with several allelic mutations. In Fusarium graminearum Schwade, however, carbendazim resistance is not associated with a mutation in the corresponding β-tubulin gene. RESULTS: The β-tubulin gene conferring carbendazim resistance in B. cinerea was cloned and connected with two homologous arms of the β-tubulin gene of F. graminearum by using a double-joint polymerase chain reaction (PCR). This fragment was transferred into F. graminearum via homologous double crossover at the site where the β-tubulin gene of F. graminearum is normally located (the β-tubulin gene of F. graminearum had been deleted). The transformants were confirmed and tested for their sensitivity to carbendazim. CONCLUSION: The β-tubulin gene conferring carbendazim resistance in B. cinerea could not express this resistance in F. graminearum, as transformants were still very sensitive to carbendazim. Copyright © 2010 Society of Chemical Industry

Gibberella zeae

10.1002/ps.1897

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Effect of Selected Plant Growth Regulators on Yield and Stem Height of Spring Wheat in Ontario

Journal of Agricultural Science (2017)

Yuxue Zhang Shengnan Su Mirko Tabori Junjie Yu Denise Chabot

Lodging is an important limiting factor in wheat because it affects growth, yield and grain quality. Plant growth regulators (PGRs) are often used to restrain elongation of internodes, improve lodging traits, and protect yield potentials. An experiment was set up in the greenhouse at the Ottawa Research and Development Centre (ORDC) to study the effect of the selected PGRs (Manipulator, the active ingredient of which is chlormequat; and Palisade, the active ingredient of which is trinexapac-ethyl) on yield, stem height and morphological traits in six spring wheat cultivars (AC Carberry, AAC Scotia, Hoffman, Fuzion, FL62R1, and AW725). Both PGRs reduced plant height and caused a 6% to 48% reduction in the length of the second basal internode. The mixture of the two PGRs had a synergistic affect and made the stem shorter. The application of PGRs significantly reduced lodging, increased stem diameter, thickness, filling degree, and stem strength, and increased leaf relative chlorophyll content. However, application of PGRs significantly reduced grain yield, and the combination of the two PGRs (Manipulator and Palisade) had a synergistic effect and lowered the yield. In general, the effect of Palisade was more evident than that of Manipulator.

Plant stem

Chlormequat

Main stem

Limiting

10.5539/jas.v9n12p30

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Genome-Wide Identification of Zn2Cys6 Class Fungal-Specific Transcription Factors (ZnFTFs) and Functional Analysis of UvZnFTF1 in Ustilaginoidea virens

Rice Science (2021)

Tianqiao Song Xiong Zhang Zhang You Dong Liang Jiaoling Yan

Transcription factors (TFs) orchestrate the regulation of cellular gene expression and thereby determine cell functionality. In this study, we analyzed the distribution of TFs containing domains, which named as ZnFTFs, both in ascomycete and basidiomycete fungi. We found that ZnFTFs were widely distributed in these fungal species, but there was more expansion of the ZnFTF class in Ascomycota than Basidiomycota. We identified 40 ZnFTFs in Ustilaginoidea virens, and demonstrated the involvement of UvZnFTF1 in vegetative growth, conidiation, pigment biosynthesis and pathogenicity. RNA-Seq analysis suggested that UvZnFTF1 may regulate different nutrient metabolism pathways, the production of secondary metabolites, and the expression of pathogen-host interaction genes and secreted protein- encoding genes. Analysis of the distribution of different fungal TFs in U. virens further demonstrated that UvZnFTFs make up a large TF family and may play essential biological roles in U. virens.

Conidiation

Secondary metabolism

Fungal protein

10.1016/j.rsci.2021.03.001

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A rapid detection method of early spore viability based on AC impedance measurement

Journal of Food Process Engineering (2020)

Ning Yang Jiaqi Hu Xu Zhou Aiying Wang Junjie Yu

Abstract Rice blast is a destructive disease of rice; it is difficult to detect the pathogenic spore viability in early stage. In this paper, a rapid detection method is proposed; the impedance‐viability model of spores was established by measuring the viability of early rice blast spores and the AC impedance at the most sensitive frequencies. This model is a nice linear model, and the goodness of fit R 2 is greater than 0.99. At the same time, there is a positive correlation between the spore impedance‐viability model and the hyphae impedance‐growth speed model measured in practice. The p value is less than 0.05, and the Pearson coefficient is close to 1, which proves the feasibility of this method to detect spore viability. Compared with the traditional experimental culture method, the detection speed of spore viability based on impedance method is greatly improved, which brings convenience for the study of rice blast. Practical Applications The viability of spores is one of the most important indexes to show the degree of rice blast. The early spores of the rice blast look similarly, which makes it hard for the naked eye to distinguish the viability, and makes it difficult for farmers to decide whether to use pesticides in the rice field. The traditional method for the detection of spore viability of rice blast is complicated and needs a long time. In recent years, the method of microbial impedance is put forward, which can provide the information of microbial AC impedance, and has the advantages of fast and nondestructive. The results showed that it is feasible to detect spore viability by impedance technique.

10.1111/jfpe.13520

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Citations (3)

Optimization of Bacillus amyloliquefaciens Lx-11 suspoemulsion by response surface methodology to control rice bacterial blight

BioControl (2023)

Hao Zhang Rongsheng Zhang Junqing Qiao Junjie Yu Zhongqiang Qi

Bacillus amyloliquefaciens

10.1007/s10526-023-10186-6

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Citations (5)