Yonghong Tian

Women's Hospital, School of Medicine, Zhejiang University

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Lejun Li

Women's Hospital, School of Medicine, Zhejiang University

Zhixiang Ren

Peng Cheng Laboratory

Zhongrui Zhao

University of Chinese Academy of Sciences

Xinze Zhang

Shanghai Industrial Technology Institute

Xiaoshuai Zhu

Chinese Academy of Sciences

Sixuan Liu

Central South University

Fengbin Zhang

Zhejiang University

Fan Jin

Women's Hospital, School of Medicine, Zhejiang University

Long Xu

Ningbo University

Chengliang Xiong

Huazhong University of Science and Technology

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Huazhong University of Science and Technology

Zhejiang University

Women's Hospital, School of Medicine, Zhejiang University

Chinese Academy of Sciences

Shanghai Jiao Tong University

Tongji Hospital

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

Yanbian University

Central South University

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NLFFF Dataset Flare Label

Figshare (2022)

Zhongrui Zhao Long Xu Xiaoshuai Zhu Xinze Zhang Sixuan Liu

NLFFF Dataset Flare Label and Intermediate Files

Flare

10.6084/m9.figshare.21760637.v1

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[Detection of Trichomonas vaginalis with direct immunofluorescence assay].

PubMed (2003)

Yonghong Tian Cheng-liang Xiong Huang-Tao Guan Pang Xuebing Jiang ChangFu

To detect Trichomonas vaginalis (Tv) by direct immunofluorescence assay (DFA) and discuss its clinical significance.The parasites at a concentration of 3 x 10(5) cells/L were fixed by acetone on slides which were then blocked by 1% BSA (bovine serum albumin) or 10% BSA or 10% NCS (newborn calf serum) respectively, incubated with different dilution of polyclonal goat anti-Tv IgG (1:20-1:2,560) for different incubation time (15, 30, 45, 60, 90, 120 min). 120 clinical vaginal specimens were examined by direct immunofluorescence assay, the wet mount method and the in vitro cultivation.Blocked by 1% or 10% BSA, incubated at 37 degrees C for 45 min with a titer 1:160 of polyclonal antibody were the optimal conditions for direct immunofluorescence assay. Its sensitivity and specificity were 87.9% and 98.6% respectively in comparison with the in vitro cultivation method.Direct immunofluorescence assay is a useful alternative to the wet mount method which shows a lower sensitivity.

Polyclonal antibodies

Immunofluorescence

Bovine serum albumin

Direct fluorescent antibody

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Candida albicans and filtrates interfere with human spermatozoal motility and alter the ultrastructure of spermatozoa: an in vitro study

Andrology (2007)

Yonghong Tian Jinwen Xiong Lian Hu Donghui Huang Chengliang Xiong

The in vitro effects of Candida albicans and its filtrates on the motility and ultrastructure of human spermatozoa from healthy donors were studied. A significant reduction in sperm progressive motility and signs of membrane alteration, directly related to contact time were observed. Distinct adhesion of spermatozoa to C. albicans and agglutination were observed. Light and transmission electron microscopy examinations showed that spermatozoa attached to C. albicans mainly via the head. Multiple ultrastructural lesions were present, which were the possible morphological reasons for spermatozoan immobilization observed in the present study. It is concluded that C. albicans as well as its filtrates had an inhibitory effect on human sperm motility and impaired the ultrastructure of human spermatozoa, which could be associated with male infertility.

10.1111/j.1365-2605.2006.00734.x

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Cloning and Analysis of the Promoter Region of Rat uPA Gene

Journal of Reproduction and Contraception (2007)

Yan Liu Jinwen Xiong Ligang Chen Yonghong Tian Chengliang Xiong

TATA box

genomic DNA

CAAT box

10.1016/s1001-7844(07)60001-7

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Citations (1)

Seminal plasma HSPA2 mRNA content is associated with semen quality

Journal of Assisted Reproduction and Genetics (2016)

Yonghong Tian Lejun Li Fengbin Zhang Jian Xu

Asthenozoospermia

Semen quality

10.1007/s10815-016-0730-4

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Clincal analysis of 135 elderly patients wish colorectal carcinoma

China Practical Medicine (2010)

Yonghong Tian

Objective To analyze and summarize the clinical characters in elderly patients with colorectal carcinoma. Methods After the patients were admitted to hospital,the abnormalities that might increase operative risk or adversely influence recovery must be treated peror to operation,and be treated postoperatively if necessary. Patients were closely monitored throughout the operative and postoperative phase of the first a few days so that any abnormalities could be treated immediately. Results Among the 135 eldely patients with colorectal carcinoma,85 cases( 62. 96% ) suffered from common diseases in the elderly,sush as hypertension,cardiac disease,diabetes et al. The rate of postoperative complications in the elderly who had coexisent disease was 26. 8% ,whereas the rate of postoperative complications in the elderly who had no coexistent diseases was 10% . There was a siginificant difference between the groups( P 0. 01) . Conclusion The coexisting diseases increase the risk of surgical procedures,but complete assessment of the patient's general health and intensive perioperaive management of the coexisting diseases can increase the success rate of operation and decrease the operative mortality and incidence of complication.

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Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa

Genetics and Molecular Research (2014)

Jie Cui Yonghong Tian Y. Liu Hongyi Huang Yi Jin

The main objective of the current study was to explore the different activation mechanisms of capacitation and freezethawed spermatozoa.Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed.The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group.Fresh spermatozoa displayed 45-and 35-kDa protein bands, while those of freeze-thawed andcapacitated spermatozoa displayed 45-, 35-and 28-kDa bands.In summary, these data indicate that proacrosin is activated, thus becoming αand β-acrosins and a 28-kDa protein during capacitation and freezethawing.

Capacitation

BOAR

10.4238/2014.november.27.20

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NLFFF Dataset and Flare Label Database Archive

Figshare (2023)

Zhongrui Zhao Long Xu Xiaoshuai Zhu Xinze Zhang Sixuan Liu

An archive of NLFFF dataset summary information in sqlite database format, which contains raw header file information, archive information of 3D magnetic fields extrapolated using Wiegelmann's Nonlinear Force-Free Field (NLFFF) extrapolation algorithm, flare information, and flare label of database samples.

Flare

10.6084/m9.figshare.21760658

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NLFFF Dataset Flare Label

Figshare (2023)

Zhongrui Zhao Long Xu Xiaoshuai Zhu Xinze Zhang Sixuan Liu

NLFFF Dataset Flare Label and Intermediate Files

Flare

10.6084/m9.figshare.21760637.v2

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