An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Objective To construct the fusion gene of human MAGE-1 and Mycobacterium tuberculosis heat shock protein 70. The fusion gene was expressed in E. coli. and the fusion protein was purified. Methods the HSP70 and MAGE-1 genes were amplified by PCR. After sequencing, the two genes were cloned into the expression vector pGEX-4T-1 to construct fusion gene expression plasmid pGEX-MAGE-1-TBHSP70. The DH5α contained the expression plasmid was induced by IPIG and the fusion protein was purified with GSTrap FF column. Results The HSP70 gene and MAGE-1 gene segment were amplified successfully from plasmids, and their sequences were identical with that reported in GenBank. The fusion gene expression plasmid pGEX-MAGE-1-TBHSP70 was successfully constructed. The DH5α contained the plasmid could express a M, 125 000 protein after induced by IPTG and the M_r 125 000 fusion protein was purified by GSTrap FF column. Conclusion In this study, the fusion protein of human MAGE-1 segment and Mycobacterium tuberculosis HSP70 was expressed and purified successfully, which prepared the materials for the research of the HSP70 protein as a adjuvant-free carrier.
A combination of methylprednisolone sodium succinate (MSS) and granisetron hydrochloride (GH) is generally devoted to treating the chemotherapy-induced nausea and vomiting. To date, none of these novel mixtures have been commercially available. The present study was aimed at investigating physical and chemical compatibility and stability of a combination of MSS with GH in 0.9% sodium chloride injection for 72 hours at 4°C and 25°C. A mixture of MSS (0.4–0.8 mg/mL) with GH (0.03 mg/mL) was prepared and stored in both polyvinyl chloride bags and glass bottles using 0.9% sodium chloride injection as a diluent. The study was performed using a validated and stability-indicating high-performance liquid chromatography method. The physical compatibility was assessed by a spectrometer. Furthermore, the pH measurement of each sample was measured electronically. All test solutions stored at 4°C or 25°C had a no >2% loss of the initial concentration throughout the 72-hour study period. All solutions remained clear and colorless throughout the study and were without precipitation or turbidity in any of the batches. The drug mixtures of MSS (0.4–0.8 mg/mL) and GH (0.03 mg/mL) in 0.9% sodium chloride injections were physically and chemically stable for at least 72 hours when stored at 4°C or 25°C in polyvinyl chloride bags or glass bottles.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Background . The cardiovascular characteristics of children with Hutchinson-Gilford progeria syndrome (HGPS) remain unclear. The present study is aimed at evaluating the cardiovascular changes with ultrasound examination in children with HGPS and compared these with those in normal children and older people. Methods . Seven HGPS children, 21 age-matched healthy children, and 14 older healthy volunteers were evaluated by three-dimensional echocardiography (including strain analysis) and carotid elasticity examination with the echo-tracking technique. Results . Children with HGPS had higher left ventricular ejection fraction (LVEF) and global longitudinal strain, when compared to older healthy volunteers (P<0.05). However, these parameters were not significantly different, when compared to those in healthy children. Furthermore, children with HGPS had lower average peak times in the left ventricle, when compared with the other two groups. For the structure of the carotid artery detected by ultrasound, the abnormality rates were similar between children with HGPS and older healthy volunteers (83.3% vs. 71.4%). The elastic parameters, elastic modulus, stiffness parameter, and pulsed wave transmittal velocity of children with HGPS were lower, when compared to those in older healthy volunteers (P<0.05), while they were higher with arterial compliance (P>0.05). Furthermore, no significant difference existed among the vascular elastic parameters between HGPS and normal children. Conclusion . HGPS children had impaired left ventricular (LV) synchrony, when compared to normal children, although the difference in LVEF was not statistically significant. Furthermore, the structural abnormality of the carotid artery in HGPS children was similar to that in older people, although the index of elasticity appears to be more favorable. These results suggest that the cardiovascular system in HGPS children differs from natural aging.
Previous studies have demonstrated that a family history of breast cancer is considered a risk factor, and hereditary factors may be involved in breast cancer pathogenesis. Next‑generation sequencing techniques were used to analyze 111 cancer‑associated genes in patients with breast cancer with a familial history of malignant tumors in the pre‑experiment and a novel variant, receptor tyrosine‑protein kinase erbB‑2 (ERBB2) c.338G>A: p.R113Q was identified in two cases of breast cancer. ERBB2 is considered an important oncogene, and overexpression or mutation of the ERBB2 gene may lead to the occurrence or metastasis of tumors. To assess a potential association between rs185670819 and breast cancer, 117 patients with breast cancer and a familial history of any cancer, who were diagnosed by experienced pathologists at the Xijing Hospital (Shaanxi, China) between July 2015 and December 2016, were recruited. The presence of the missense variant was confirmed using bi‑directional Sanger sequencing of samples from the patients with breast cancer and 250 healthy controls. The effects of the missense mutation on the structure and function of ERBB2 were analyzed in silico. The missense variant, R113Q, in patients with breast cancer with a familial history of malignant tumors in China, was present in 8 patients [6.8% (95% CI: 3.21‑13.45)] and 3 of 250 healthy controls [1.2% (95% CI: 0.31‑3.76; OR=6.04, 95% CI: 1.573‑23.214, P=0.009)]. Of the 8 patients with the R113Q variant, 6 patients had a family history of cancer of the digestive system. The present study suggests that ERBB2 c.338G>A: p.R113Q may be a potential risk factor in the development and progression of breast cancer.
AIM: To construct the melanoma antigen-1(MAGE-1) eukaryotic expression plasmid and express MAGE-1 in mouse melanoma B16 cells. METHODS: The MAGE-1 gene was amplified by PCR and cloned into the eukaryotic expressi on vector pIRES2-EGFP to construct the pIRES2-EGFP-MAGE-1 plasmid. The plasm id was transfected into the B16 cells. The EGFP expression was detected under f luoroscent microscope and the MAGE-1 expression was detected by immunohistoche mistry staining. RESULTS: The eukaryotic expression vector pIRE S2-EGFP-MAGE-1 was constructed and transfected successfully into B16 cells, a nd the EGFP and MAGE-1 genes were co-expressed in the B16 cells. CONCL USION: A mouse melanoma cell line B16 co-expressing MAGE-1 and EGFP genes has been established successfully, which lays the foundation for the rese arch on application of MAGE-1 in the tumor immunotherapy.
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