A method for isolation of the nuclei from embryo of dormant barley seed is described. In them, metaphasic chromosome figures were found, and somatic association of metaphasic chromosomes was repeatedly observed.

Isolation

10.1266/jjg.55.77

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Citations (2)

The Inhibition of Division in Barley Cells by Antibiotics and the Effect of Gamma-rays on Synthesis of Division Protein

Ikushugaku zasshi (1974)

Hikoyuki YAMAGUCHI Atsushi Tatara

Several antibiotics, being effective inhibitors of protein and messenger RNA, such as chloramphenicol, puromycin, cycloheximide and actinomycin D, were administered to the soaking barley seeds during premitotic interphase of first leaf meristem cells. Consequently, it was noted that some kind or degree of protein and messenger RNA which were necessary for the beginning of mitosis were synthesized concurrently with DNA replication. By using microautoradiographic method with incorporation of tritiated leucine soon after exposure of gamma-rays, however, it was found that the biosynthesis of the protein essential for mitosis was scarcely inhibited with relatively low doses (less than 1, 800 R) .

Puromycin

Interphase

10.1270/jsbbs1951.24.141

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Citations (1)

Decreased activity of DNA polymerase in seeds of barley during storage.

The Japanese Journal of Genetics (1978)

Hikoyuki YAMAGUCHI Tadao NAITO Atsushi Tatara

10.1266/jjg.53.133

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Citations (15)

DNA Synthesis during Premitotic (G2) and Mitotic (M) Phases in the Meristematic Cells of Barley

RADIOISOTOPES (1972)

Hikoyuki YAMAGUCHI Atsushi Tatara

10.3769/radioisotopes.21.7_433

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Citations (4)

Detection of 3′-OH broken termini of DNA strands using the TdT reaction

Mutation Research/Environmental Mutagenesis and Related Subjects (1995)

Atsushi Tatara Hironobu Eguchi Kei Kobayashi Kotaro Hieda Takashi Ito

10.1016/0165-1161(95)90165-5

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Citations (1)

UNSCHEDULED DNA SYNTHESIS INDUCED IN BARLEY SEEDS BY GAMMA RAYS AND 4-NITROQUINOLINE 1-OXIDE

The Japanese Journal of Genetics (1975)

Hikoyuki YAMAGUCHI Atsushi Tatara Tadao NAITO

During the early stage of germination of barley seeds the nuclear chromatin, which was diffused fibrously in the first-leaf and root meristem cells, incorporated tritiated thymidine into them. These tritium-labeled nuclei increased significantly after the treatments of gamma rays and 4-nitro-quinoline 1-oxide (4NQO). With post-treatment of 1mM EDTA, radiation-induced incorporation of tritiated thymidine into the diffuse nuclei of irradiated seeds was inhibited. The results obtained suggest that unscheduled DNA synthesis occurs in accordance with or due to chromatin diffusion in barley cells after gamma-ray irradiation and treatment with 4NQO.

4-Nitroquinoline 1-oxide

Thymidine

10.1266/jjg.50.307

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Citations (23)

Induction of single-strand DNA breaks with synchrotron radiation in dry Barley nucleus

Environmental and Experimental Botany (1982)

H. Yamaguchi Atsushi Tatara Yu Sato H. Eguchi Takashi Ito

10.1016/0098-8472(82)90046-6

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