Ha-Hyun Kim

Animal and Plant Quarantine Agency

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Dong-Kun Yang

Animal and Plant Quarantine Agency

Bang‐Hun Hyun

Animal and Plant Quarantine Agency

Kyoung‐Oh Cho

Chonnam National University

Sung-Suk Choi

Animal and Plant Quarantine Agency

Jae‐Young Song

Rural Development Administration

In‐Soo Cho

Animal and Plant Quarantine Agency

Sang‐Ik Park

Chonnam National University

Mun-Il Kang

Chonnam National University

Jin‐Ju Nah

Animal and Plant Quarantine Agency

Jae Young Yoo

Korea Disease Control and Prevention Agency

Cooperative Institutions

Animal and Plant Quarantine Agency

Ministry of Agriculture, Food and Rural Affairs

Chonnam National University

Korea Research Institute of Bioscience and Biotechnology

National Veterinary Research and Quarantine Service

Chonnam National University Hospital

Seoul National University

Yonsei University

Kangwon National University

Kyungpook National University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Antibody Response in Korean Raccoon Dogs Inoculated with Inactivated Rabies Vaccines

Journal of Bacteriology and Virology (2012)

Dong-Kun Yang T. Go Young‐Hee Nam Ha-Hyun Kim Soo-Dong Cho

Since sylvatic rabies was first identified in South Korea in 1993, over three million bait vaccine doses have been distributed to rabies risk regions in order to block transmission of rabies among wild animals. New progressive strategy is needed to eliminate sylvatic rabies completely in rabies risk regions. Before applying the preventive program related to eradication, immunogenicity of inactivated rabies vaccines available in Korea has to be evaluated in Korean raccoon dogs (Nyctereutes procyonoides koreensis). Six groups of raccoon dogs in wild rescue center of Gyeonggi-do were vaccinated intramuscularly with single dose of six inactivated commercial rabies vaccines (designated A to F). Serum samples at the time of vaccination, and two and four weeks post vaccination were obtained and analyzed by virus neutralizing assay (VNA). All raccoon dogs inoculated with vaccines C, D, E or F, showed VN antibody titers ranging from 0.5 to 13.77 IU/ml. Half of four raccoon dogs immunized with vaccine B revealed VN titer over 0.5 IU/ml, and one of four raccoon dogs inoculated with vaccine A showed protective antibody titer. This finding suggests that most of the commercially available inactivated rabies vaccines could induce protective immunity in Korean raccoon dogs and be applicable to new rabies control program.

Raccoon Dogs

Rabies vaccine

Inactivated vaccine

Antibody titer

10.4167/jbv.2012.42.3.242

Cite

Citations (7)

Detection of Neutralizing Antibody Against Japanese Encephalitis Virus in Wild Boars of Korea

Journal of Bacteriology and Virology (2012)

Dong-Kun Yang Ha-Hyun Kim Bang‐Hun Hyun Seong-In Lim Yun-Kyoung Nam

Several species of animals, including horses and pigs, can be infected with Japanese encephalitis virus (JEV). Wild boars (Sus scrofa) are also considered to be an effective amplifying host for JEV in wild environments. In this study, 288 blood samples were collected from wild boars in eight Korean provinces, and antibodies against JEV were detected using a virus neutralizing assay. The results showed that 66.0% (190/288) of wild boars in Korea had neutralizing antibodies against JEV. We found no significant differences in the seroprevalence of JEV among provinces (p > 0.05). The results indicate that wild boars in Korea have been exposed to JEV, suggesting that these boars may play an important role in amplifying and carrying JEV to other regions of Korea. The result of this study may be helpful for planning preventive measures.

Seroprevalence

10.4167/jbv.2012.42.4.353

Cite

Citations (7)

Pathogenicity of porcine G9P[23] and G9P[7] rotaviruses in piglets

Veterinary Microbiology (2013)

Ha-Hyun Kim Jun‐Gyu Park Jelle Matthijnssens Hyun Jeong Kim Hyung‐Jun Kwon

Viremia

Mesenteric lymph nodes

Intestinal mucosa

Viral Shedding

10.1016/j.vetmic.2013.05.024

Cite

Citations (16)

Pathological Study on Interstitial Pneumonia in Korean Native Goats

Laboratory Animal Research (2007)

Ha-Hyun Kim In-Jin Hwang Su‐Jin Park Sang‐Ik Park Young‐Ju Jeong

Parenchyma

Alveolar Wall

Infiltration (HVAC)

Anorexia

Diffuse alveolar damage

Source

Cite

Citations (0)

Inactivated genotype 1 Japanese encephalitis vaccine for swine

Clinical and Experimental Vaccine Research (2014)

Dong-Kun Yang Jin‐Ju Nah Ha-Hyun Kim Jae‐Young Song

Japanese encephalitis is a reproductive disorder caused by Japanese encephalitis virus (JEV) in swine. Recent genotype (G) shift phenomenon (G3 to G1) in the Asia-wide has posed a challenge for proper prevention by the current vaccine strain. Thus, new kinds of JEV G1 vaccines with enhanced immunogenicity have been required for pigs.Recombinant porcine granulocyte monocyte-colony stimulating factor (reporGM-CSF) protein was expressed in Spodoptera frugiperda (Sf-9) cells using baculovirus expression system. Two kinds of trials with inactivated JEV vaccines containing IMS1313 adjuvant (Seppic, France) were prepared with or without reporGM-CSF protein. Safety and immunogenicity of the pigs inoculated with the JEV vaccines via intramuscular route was evaluated for 28 days after inoculation.Mice, guinea pigs, and fattening pigs inoculated with the inactivated vaccine showed no signs for 14 and 21 days. Both hemagglutination inhibition and plaque reduction neutralizing antibody titers were significantly higher in pigs immunized with the vaccine containing reporGM-CSF protein after boosting. However, on the side of vaccine efficacy, most mice (87%) immunized with the inactivated JEV vaccine survived after virulent JEV challenge. Whereas the group with the vaccine containing reporGM-CSF protein showed lower protective effects than the vaccine alone for the biological activity of the GM-CSF depending on species specific.Our data indicate that animals inoculated with the JEV vaccines was safe and pigs inoculated with inactivated JEV vaccine containing reporGM-CSF protein showed higher humoral immune responses than that of inactivated JEV vaccine without reporGM-CSF protein.

Inactivated vaccine

Japanese encephalitis vaccine

10.7774/cevr.2014.3.2.212

Cite

Citations (10)

Efficacy of inactivated variant porcine epidemic diarrhea virus vaccines in growing pigs

Clinical and Experimental Vaccine Research (2018)

Seung Heon Lee Dong-Kun Yang Ha-Hyun Kim In‐Soo Cho

Purpose:The first aim of this study was to develop a novel inactivated porcine epidemic diarrhea virus (PEDV) vaccine using the recently isolated Korean PEDV QIAP1401 strain and to evaluate its protective efficacy in growing pigs.The second was to determine the optimum adjuvant formulation of the inactivated PEDV vaccine that induces protection against viral challenge.Materials and Methods: To generate high titers of infectious PEDV, the QIAP1401 isolate was passaged in Vero cells.The experimental vaccines were prepared from a binary ethyleneimineinactivated QIAP1401 strain passaged sequentially 70 times (QIAP1401-p70), formulated with four commercial adjuvants, and administered twice intramuscularly to growing pigs.Challenge studies using a virulent homologous strain of PEDV QIAP1401-p11, which was passaged 11 times after isolation, were performed to assess protection against disease progression and viral shedding during the 15-day observation period.The vaccine-induced antibody responses were measured in serum samples collected at predetermined time points by indirect enzymelinked immunosorbent assay and virus neutralization test.Results: The QIAP1401-p70 strain had 42 amino acid (aa) mutations, including a 25 aa deletion, and was selected as the inactivated PEDV vaccine candidate.Although none of the pigs that received the experimental vaccines were completely protected against subsequent viral challenge, they exhibited a significantly higher immune response than did non-vaccinated control pigs.Among the vaccine groups, the highest antibody responses were observed in the pigs that received an oil-based multiphasic water/oil/water (W/O/W) emulsion adjuvanted vaccine, which delayed the onset of clinical symptoms and viral shedding.Conclusion: A novel inactivated PEDV vaccine formulated with a W/O/W emulsion adjuvant was both immunogenic and protective against viral challenge.

Inactivated vaccine

Viral Shedding

Vero cell

Vaccine efficacy

10.7774/cevr.2018.7.1.61

Cite

Citations (30)

Immunogenicity of an inactivated rabies vaccine for animals derived from the recombinant ERAGS strain

Clinical and Experimental Vaccine Research (2021)

Dong-Kun Yang Ha-Hyun Kim Yu‐Ri Park Jae Young Yoo Yeseul Park

The aims of the present study were to evaluate the immunogenicity of an inactivated rabies vaccine based on the ERAGS strain.The ERAGS virus propagated in Vero cells was inactivated with 3 mM binary ethylenimine for 8 hours. Three types of inactivated rabies vaccines were prepared to determine the minimum vaccine virus titers. Four further types of inactivated rabies vaccines were prepared by blending inactivated ERAGS with four different adjuvants; each vaccine was injected into mice, guinea pigs, and dogs to identify the optimal adjuvant. The immunogenicity of a Montanide (IMS) gel-adjuvanted vaccine was evaluated in cats, dogs, and cattle. Humoral immune responses were measured via a fluorescent antibody virus neutralization method and a blocking enzyme-linked immunosorbent assay.The minimum virus titer of the inactivated rabies vaccine was over 107.0 50% tissue culture infectious doses (TCID50 values)/mL. Of the four kinds of adjuvants, the IMS gel-adjuvanted vaccine induced the highest mean viral neutralizing antibody (VNA) titers of 6.24 and 2.36 IU/mL in guinea pigs and dogs, respectively, and was thus selected as the vaccine for the target animals. Cats, dogs, and cattle inoculated with the IMS gel-adjuvanted vaccine developed protective VNA titers ranging from 3.5 to 1.2 IU/mL at 4 weeks post-inoculation (WPI).Our data indicate that cats, dogs, and cattle inoculated with an inactivated rabies vaccine derived from the ERAGS strain developed protective immune responses that were maintained to 12 WPI.

Rabies vaccine

Duck embryo vaccine

Inactivated vaccine

Vero cell

Antibody titer

10.7774/cevr.2021.10.2.141

Cite

Citations (2)

Recharacterization of Morphological and Genetic Feature of Getah Virus Isolated from South Korea

Journal of Bacteriology and Virology (2015)

Seung Heon Lee Dong-Kun Yang Ha-Hyun Kim Hyun-Ye Jo Sung-Suk Choi

Three QIAG93 strains, QIAG9301, QIAG9302 and QIAG9303 that have been identified as Getah virus (GETV) are analyzed in this study. The morphological features of three virus isolates were observed by using electron microscopy, suggesting that the QIAG9301, QIAG9302 and QIAG9303 isolate can be classified as tentative member of Alphavirus species in the Semliki Forest complex. The full length of the structural polyprotein gene of each QIAG93 isolate (QIAG9301, QIAG9302 and QIAG9303) was determined that are identical in size, comprising 3759 nucleotides that encoded 1253 amino acids. The sequence analysis of the structural polyprotein gene, including the C, E3, E1, 6K and E2 domain, showed that each QIAG93 isolate shares >98.9% sequence identity. The phylogenetic analysis and evolutionary distance (ED) estimation based on the structural polyprotein gene sequence showed that the QIAG9301 isolate is closely related to GETV South Korea strain (99.9% sequence identity and ED value 0.001) and Chinese GETV YN0540 strain (99.3% sequence identity ED value 0.007) than other Alphavirus species analyzed in this study. Both QIAG9032 and QIAG9303 isolate exhibited genetically close relationship with Mongolian GETV LEIV17741MPR strain (at least 99.3% sequence identity and mean ED value 0.0065). Therefore, our findings will be valuable for molecular epidemiological analyses of GETV in Korea and contribute to a further study on pathogenicity of three QIAG93 isolates in animals.

Strain (injury)

Sequence (biology)

10.4167/jbv.2015.45.4.328

Cite

Citations (3)

Oral immunization of mice with recombinant rabies vaccine strain (ERAG3G) induces complete protection

Clinical and Experimental Vaccine Research (2015)

Dong-Kun Yang Ha-Hyun Kim Sung-Suk Choi Jong-Taek Kim Woong-Ho Jeong

New rabies vaccine bait for both pets and raccoon dogs residing in Korea is needed to eradicate rabies infection among animals. In this study, we constructed a recombinant rabies virus (RABV), the ERAG3G strain, using a reverse genetics system. Then we investigated the efficacy of this strain in mice after oral administration and the safety of this strain in cats after intramuscular administration.The ERAG3G strain was rescued in BHK/T7-9 cells using the full-length genome mutated at the amino acid position 333 of the glycoprotein gene of RABV and helper plasmids. Four-week-old mice underwent one or two oral administrations of the ERAG3G strain and were challenged with the highly virulent RABV strain CVSN2c 14 days after the second administration. Clinical symptoms were observed and body weights were measured every day after the challenge.All mice showed complete protection against virulent RABV. In addition, cats intramuscularly inoculated with the ERAG3G strain showed high antibody titers ranging from 2.62 to 23.9 IU/mL at 28-day postinoculation.The oral immunization of the ERAG3G strain plays an important role in conferring complete protection in mice, and intramuscular inoculation of the ERAG3G strain induces the formation of anti-rabies neutralizing antibody in cats.

Rabies vaccine

Strain (injury)

Antibody titer

Duck embryo vaccine

10.7774/cevr.2015.4.1.107

Cite

Citations (8)

Development of a Blocking ELISA Method for Detection of Getah Virus Antibodies in Horse Sera

Journal of Bacteriology and Virology (2023)

Dong-Kun Yang Yu‐Ri Park Ha-Hyun Kim Hye Jeong Lee Subin Oh

Recent climate change and global warming are likely to increase mosquito-borne diseases. Getah virus (GETV) is a mosquito-borne virus that infects horses and other mammals, including humans. Currently, GETV infection in horse sera is confirmed by the virus neutralization (VN) test. Therefore, there is a need for a new enzyme-linked immunosorbent assay (ELISA) to detect GETV-antibody in a large number of horse serum samples. We aimed to develop a blocking ELISA (B-ELISA) method for the specific detection of GETV antibodies in horse serum samples. Antibodies against GETV in the sera of 175 horses were measured using the VN test. The purified QIAG9301-100P virus was used as an antigen for the B-ELISA. A monoclonal antibody (1E1) was conjugated with horseradish peroxidase and used as a detection antibody. For the establishment of the B-ELISA, antigen concentration, serum dilution factor, and conjugate dilution concentration were determined as 5 µg/mL, 20 times, and 3.6 µg/mL, respectively. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using sera from 175 horses. The B-ELISA had a diagnostic sensitivity of 91.4%, a specificity of 94.0%, and an accuracy of 93.1% compared with that of the VN test. The B-ELISA was significantly correlated with the VN test (r = 0.83). The new B-ELISA could replace the VN test and be useful for the sero-surveillance of GETV in horse sera. It is also expected to be able to detect GETV antibodies in the serum of various animals including pigs.

Horseradish peroxidase

10.4167/jbv.2022.53.2.170

Cite

Citations (0)