I. S. Sheoran

University of Toronto

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Randhir Singh

Central University of Punjab

Randhir Singh

Guru Angad Dev Veterinary and Animal Sciences University

V. K. Sawhney

University of Saskatchewan

Y. P. Luthra

Chaudhary Charan Singh Haryana Agricultural University

M. S. Kuhad

Chaudhary Charan Singh Haryana Agricultural University

Andrew R. S. Ross

Fisheries and Oceans Canada

Douglas J. H. Olson

Children's Hospital of Orange County

A. S. Nandwal

Sugarcane Breeding Institute

R. S. Hooda

Chaudhary Charan Singh Haryana Agricultural University

D.A.R. Sanders

Arrowhead Pharmaceuticals (United States)

Cooperative Institutions

Chaudhary Charan Singh Haryana Agricultural University

University of Saskatchewan

Punjab Agricultural University

University of Toronto

Institut des Sciences des Plantes de Montpellier

Indian Council of Agricultural Research

Plant Biotechnology Institute

Banaras Hindu University

Central Soil Salinity Research Institute

University of Delhi

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Nitrogen Fixation and Carbon Metabolism in Nodules of Pigeonpea (Cajanus cajan L.) Under Drought Stress

Journal of Plant Physiology (1988)

I. S. Sheoran Amarjit Kaur Randhir Singh

Cajanus

Isocitrate dehydrogenase

Hexokinase

Carbohydrate Metabolism

Malate dehydrogenase

10.1016/s0176-1617(88)80067-1

Cite

Citations (10)

Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajanus cajan L.)

Photosynthesis Research (1990)

I. S. Sheoran H. R. Singal Randhir Singh

Cajanus

Stomatal Conductance

Plant Physiology

10.1007/bf00034865

Cite

Citations (298)

Nitrogen fixation, photosynthesis and enzymes of ammonia assimilation and ureide biogenesis in nodules of mungbean (Vigna radiata) grown in presence of cadmium.

Indian Journal of Experimental Biology (1990)

Veena Sawhney I. S. Sheoran Randhir Singh

Radiata

Nitrogen Assimilation

Assimilation (phonology)

Source

Cite

Citations (23)

Effect of cadmium and nickel on mobilisation of food reserves and activities of hydrolytic enzymes in germinating pigeon pea seeds

Biologia Plantarum (1993)

Narsi R. Bishnoi I. S. Sheoran Randhir Singh

Inclusion of cadmium as cadmium chloride and nickel as nickel chloride in media differentially induced dry matter mobilisation from cotyledons of germinating pigeon pea seeds. Cadmium depressed the activities of total amylase, protease, acid phosphatase and peroxidase in germinating seeds. The activities of these enzymes were stimulated at the lower concentrations of nickel and suppressed at the higher ones. This dual response of hydrolytic enzymes to cadmium and nickel is postulated to account for the promotory and inhibitory effects of these heavy metals on dry matter mobilisation.

Cadmium chloride

10.1007/bf02928036

Cite

Citations (37)

Anthocyanin accumulation in the hypocotyl of an ABA‐over producing male‐sterile tomato (Lycopersicon esculentum) mutant

Physiologia Plantarum (2006)

I. S. Sheoran Tim Dumonceaux Raju Datla V. K. Sawhney

Anthocyanin accumulation is known to be regulated by light and plant hormones but its occurrence varies with plant species and/or organ and tissue, and it has been negatively correlated with male sterility. In this study, we have examined the light responsive changes in anthocyanin in an abscisic acid (ABA) over‐producer, male‐sterile 7B‐1 mutant and wild‐type (WT) tomato hypocotyls. The results show that light‐induced anthocyanin accumulation in the hypocotyl was more in WT compared with the 7B‐1 mutant and more so under white light (W) compared with blue light (B) or red light (R). In contrast, the chlorophyll content was greater in the mutant than in WT. Exogenous ABA caused a transitory increase in anthocyanin content in WT but a reduction in 7B‐1 , both in W and B. The high level of anthocyanin in WT under light conditions was not correlated with increased mRNA levels of phenylalanine ammonia‐lyase (PAL), chalcone synthase (CHS) and dihydroflavonol 4‐reductase (DFR), some of the anthocyanin biosynthetic genes. However, the activity of PAL (EC 4.3.1.5) was higher in the WT than in 7B‐1 hypocotyls, and exogenous ABA caused an increase in PAL activity in the WT but a reduction in the mutant. The results presented show that high ABA negatively affects anthocyanin accumulation and that in the 7B‐1 mutant it is related, in part, to reduced PAL activity. The results also support the view that the 7B‐1 mutant has a defect in light perception and ABA sensitivity.

Lycopersicon

Phenylalanine ammonia-lyase

Wild type

10.1111/j.1399-3054.2006.00697.x

Cite

Citations (26)

Effect of water stress on the enzymes of nitrate metabolism in two Brassica species

Phytochemistry (1979)

Pankaj Gupta I. S. Sheoran

Osmotic shock

10.1016/0031-9422(79)83078-2

Cite

Citations (10)

Carbon metabolism in leaves of cadmium treated wheat seedlings

Plant Physiology and Biochemistry (1992)

D. S. Malik I. S. Sheoran R. P. Singh

Carbon fibers

Plant metabolism

Source

Cite

Citations (92)

Preliminary X-ray crystallographic studies of UDP-glucose-4-epimerase fromAspergillus nidulans

Acta Crystallographica Section F Structural Biology and Crystallization Communications (2011)

S.A. Dalrymple I. S. Sheoran Susan G. W. Kaminskyj D.A.R. Sanders

UDP-glucose-4-epimerase (GALE) from Aspergillus nidulans was overexpressed in Escherichia coli, purified via His-tag affinity chromatography and cocrystallized with UDP-galactose using the microbatch method. The crystals diffracted to 2.4 Å resolution using synchrotron radiation on the Canadian Light Source 08ID-1 beamline. Examination of the data with d*TREK revealed nonmerohedral twinning, from which a single lattice was ultimately extracted for processing. The final space group was found to be C2, with unit-cell parameters a = 66.13, b = 119.15, c = 161.42 Å, β = 98.48°. An initial structure solution has been obtained via molecular replacement employing human GALE (PDB entry 1hzj) as a template model.

Aspergillus nidulans

Molecular replacement

10.1107/s1744309111020914

Cite

Citations (1)

Combining Rational Design and Continuous Evolution on Minimalist Proteins That Target DNA

Jumi A. Shin Ichiro Inamoto I. S. Sheoran Serban Popa Montdher Hussain

We designed MEF to mimic the basic region/helix-loop-helix/leucine zipper (bHLHZ) domain of transcription factors Max and Myc, which bind with high DNA sequence specificity and affinity to the E-box motif (enhancer box, CACGTG). To make MEF, we started with our rationally designed ME47, a hybrid of the Max basic region and E47 HLH, that effectively inhibited tumor growth in a mouse model of breast cancer. ME47, however, displays propensity for instability and misfolding. We therefore sought to improve ME47's structural and functional features. We used phage-assisted continuous evolution (PACE) to uncover "nonrational" changes to complement our rational design. PACE mutated Arg12 that contacts the DNA phosphodiester backbone. We would not have rationally made such a change, but this mutation improved ME47's stability with little change in DNA-binding function. We mutated Cys29 to Ser and Ala in ME47's HLH to eliminate undesired disulfide formation; these mutations reduced E-box binding activity. To compensate, we fused the designed FosW leucine zipper to ME47 to increase the dimerization interface and improve protein stability and E-box targeting activity. This "franken-protein" MEF comprises the Max basic region, E47 HLH, and FosW leucine zipper—plus mutations that arose during PACE and rational design—and is a tractable, reliable protein in vivo and in vitro. Compared with ME47, MEF gives three-fold stronger binding to Ebox with four-fold increased specificity for E-box over nonspecific DNA. Generation of MEF demonstrates that combining rational design and continuous evolution can be a powerful tool for designing proteins with robust structure and strong DNA-binding function. <br>

Zipper

Rational design

Transcription

10.26434/chemrxiv.11882142

Cite

Citations (0)

Effect of salt and water stress on RNA synthesis in the excised embryo axis of germinating mung bean (Phaseolus aureus ROXB).

Biologia Plantarum (1978)

I. S. Sheoran Ram K. Sihag

The rate of synthesis of RNA was studied under salt and water stresses created by the addition of four different salts and polyethylene glycol-6000 (PEG-6000) in excised embryo axis of mung bean usingl4C-uracil. Iso-osmotio concentrations of salts and PEG resulted in differential effect on RNA synthesis. PEG inhibited, whereas salt stimulated incorporation of labelled uracil into RNA. Ionic differences on RNA synthesis were very clear as synthesis of RNA was more affected with sulphate than chloride and with sodium than potassium ions.

Uracil

Mung bean

10.1007/bf02923338

Cite

Citations (2)