Frank Kirstein

University of Cape Town

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Frank Brombacher

Wellcome Centre for Infectious Diseases Research in Africa

Sabelo Hadebe

South African Medical Research Council

William Horsnell

University of Exeter

Natalie E. Nieuwenhuizen

University of Würzburg

Pierre Redelinghuys

University of Aberdeen

Kaat Fierens

Ghent University Hospital

Bart N. Lambrecht

VIB-UGent Center for Inflammation Research

Rebecca A. Drummond

University of Birmingham

David L. Williams

East Tennessee State University

Simon Vautier

University of Aberdeen

Cooperative Institutions

University of Cape Town

International Centre for Genetic Engineering and Biotechnology

South African Medical Research Council

Wellcome Centre for Infectious Diseases Research in Africa

University of Aberdeen

International Centre for Genetic Engineering and Biotechnology

Red Cross War Memorial Children's Hospital

Council for Scientific and Industrial Research

National Institutes of Health

Medical Research Council

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

M3R deficient mice have impaired memory immune responses during secondary infection with N. brasiliensis .

Matthew Darby Corinna Schnoeller Alykhan Vira Fiona J. Culley Saeeda Bobat

10.1371/journal.ppat.1004636.g004

Cite

Citations (0)

β-Glucan and LPS influence T-cell responses to HDM.

Hadebe Sabelo Frank Kirstein Kaat Fierens Kong Chen A. Drummond Rebecca

10.1371/journal.pone.0134219.g004

Cite

Citations (0)

Residual allergenicity of amino acid-based and extensively hydrolysed cow’s milk formulas

South African Medical Journal (2017)

Michael Levin Dee Blackhurst Frank Kirstein D. M. KOK George F. van der Watt

Criteria for labelling infant feeds as suitable for the dietary management of cow's milk protein allergy (CMPA) rely on proving the hypoallergenicity of such feeds or clinical studies showing that the feeds are tolerated by 90% of children with proven CMPA. South African (SA) labelling legislation does not indicate what testing is necessary to prove hypoallergenicity.To evaluate all extensively hydrolysed cow's milk formulas and amino acid-based formulas available in SA for residual allergen content, protein size and amino-acid content.All amino-acid and extensively hydrolysed formulas were found to be similar in composition, with no residual cow's milk allergens detectable by enzyme-linked immunosorbent assay. Furthermore, proteins were absent and only small molecules in the size range of amino acids and possibly of very small oligopeptides were detected.These findings indicate that the formulas are extremely likely to be compliant with the definition of hypoallergenicity as tolerance in 90% of proven sufferers from cow's milk allergy. The formulas may therefore be labelled as suitable for the dietary management of infants with CMPA.

Hydrolyzed protein

Milk protein

Cow milk

10.7196/samj.2017.v107i9.12137

Cite

Citations (11)

Investigation of in vitro and in vivo anti-asthmatic properties of Siphonochilus aethiopicus

Journal of Ethnopharmacology (2010)

Gerda Fouché Natalie E. Nieuwenhuizen Vinesh Maharaj Schalk van Rooyen Nial Harding

Expectorant

10.1016/j.jep.2010.11.014

Cite

Citations (22)

IL-4Rα-responsive smooth muscle cells contribute to initiation of TH2 immunity and pulmonary pathology in Nippostrongylus brasiliensis infections

Mucosal Immunology (2010)

William Horsnell Alykhan Vira Frank Kirstein Helen Mearns J. Claire Hoving

Nippostrongylus brasiliensis infections generate pulmonary pathologies that can be associated with strong T(H)2 polarization of the host's immune response. We present data demonstrating N. brasiliensis-driven airway mucus production to be dependent on smooth muscle cell interleukin 4 receptor-α (IL-4Rα) responsiveness. At days 7 and 10 post infection (PI), significant airway mucus production was found in IL-4Rα(-/lox) control mice, whereas global knockout (IL-4Rα(-/-)) and smooth muscle-specific IL-4Rα-deficient mice (SM-MHC(Cre) IL-4Rα(-/lox)) showed reduced airway mucus responses. Furthermore, interleukin (IL)-13 and IL-5 cytokine production in SM-MHC(Cre) IL-4Rα(-/lox) mice was impaired along with a transient reduction in T-cell numbers in the lung. In vitro treatment of smooth muscle cells with secreted N. brasiliensis excretory-secretory antigen (NES) induced IL-6 production. Decreased protein kinase C (PKC)-dependent smooth muscle cell proliferation associated with cell cycle arrest was found in cells stimulated with NES. Together, these data demonstrate that both IL-4Rα and NES-driven responses by smooth muscle cells make important contributions in initiating T(H)2 responses against N. brasiliensis infections.

Nippostrongylus brasiliensis

Interleukin 13

10.1038/mi.2010.46

Cite

Citations (31)

Microbial Ligand Costimulation Drives Neutrophilic Steroid-Refractory Asthma

PLoS ONE (2015)

Sabelo Hadebe Frank Kirstein Kaat Fierens Kong Chen Rebecca A. Drummond

Asthma is a heterogeneous disease whose etiology is poorly understood but is likely to involve innate responses to inhaled microbial components that are found in allergens. The influence of these components on pulmonary inflammation has been largely studied in the context of individual agonists, despite knowledge that they can have synergistic effects when used in combination. Here we have explored the effects of LPS and β-glucan, two commonly-encountered microbial agonists, on the pathogenesis of allergic and non-allergic respiratory responses to house dust mite allergen. Notably, sensitization with these microbial components in combination acted synergistically to promote robust neutrophilic inflammation, which involved both Dectin-1 and TLR-4. This pulmonary neutrophilic inflammation was corticosteroid-refractory, resembling that found in patients with severe asthma. Thus our results provide key new insights into how microbial components influence the development of respiratory pathology.

Pathogenesis

10.1371/journal.pone.0134219

Cite

Citations (42)

β-Glucan plus LPS promote neutrophilic inflammation in OVA-induced allergic inflammation.

Sabelo Hadebe Frank Kirstein Kaat Fierens Kong Y. Chen Rebecca A. Drummond

Allergic Inflammation

10.1371/journal.pone.0134219.g002

Cite

Citations (0)

Differential responses to natural and recombinant allergens in a murine model of fish allergy

Molecular Immunology (2010)

Michelle L. van der Ventel Natalie E. Nieuwenhuizen Frank Kirstein Christoph Hikuam Mohamed F. Jeebhay

10.1016/j.molimm.2010.11.001

Cite

Citations (53)

β-Glucan exacerbates allergic airway responses to house dust mite allergen

Respiratory Research (2016)

Sabelo Hadebe Frank Kirstein Kaat Fierens Pierre Redelinghuys Graeme I. Murray

β-(1,3)-Glucan is present in mould cell walls and frequently detected in house dust mite (HDM) faeces. β-Glucan exposure is thought to be associated with pulmonary allergic inflammation in mouse and man, although the published data are inconsistent. Here, we show that highly purified β-glucan exacerbates HDM-induced eosinophilic, T helper 2 type airway responses by acting as an adjuvant, promoting activation, proliferation and polarisation of HDM-specific T cells (1-Derβ T cells). We therefore provide definitive evidence that β-glucan can influence allergic pulmonary inflammation.

Aeroallergen

Allergic Inflammation

Allergic response

10.1186/s12931-016-0352-5

Cite

Citations (22)

Therapeutic and prophylactic deletion of IL‐4Ra‐signaling ameliorates established ovalbumin induced allergic asthma

Allergy (2019)

Jermaine Khumalo Frank Kirstein Martyna Scibiorek Sabelo Hadebe Frank Brombacher

Abstract Background Allergic asthma is a chronic inflammatory airway disease driven predominantly by a T H 2 immune response to environmental allergens. IL‐4Rα‐signaling is essential for driving T H 2‐type immunity to allergens. Anti‐T H 2 therapies have the potential to effectively reduce airway obstruction and inflammation in allergic asthma. Objective We investigated potential therapeutic effects of selective inhibition of this pathway in mice with established allergic airway disease. We further investigated whether IL‐4Rα disruption in systemically sensitized mice can prevent the onset of the disease. Methods We used Rosa creERT2 IL‐4Rα −/lox mice, a tamoxifen (TAM)‐inducible IL‐4Rα knockdown model to investigate the role of IL‐4/IL‐13 signaling prior to the onset of the disease and during the effector phase in the ovalbumin‐induced allergic airway disease. Results Inducible deletion of IL‐4Rα demonstrated therapeutic effects, on established allergic airway disease, and prevented the development of ovalbumin‐induced airway hyperreactivity, eosinophilia, and goblet cell metaplasia in allergen‐sensitized mice. Interestingly, IL‐4Rα knockdown after allergic sensitization did not induce T H 17, a neutrophilic inflammatory response as observed in global IL‐4Rα‐deficient mice after intranasal allergen challenge. Conclusion Abrogation of IL‐4Rα signaling after allergic sensitization would have significant therapeutic benefit for T H 2‐type allergic asthma.

Allergic Inflammation

Allergic response

10.1111/all.14137

Cite

Citations (11)