Shicong Zheng

Guangzhou Medical University

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Yongchang Yao

First Affiliated Hospital of Guangzhou Medical University

Jianyu Zou

First Affiliated Hospital of Jinan University

Shujiang Zhang

Institute of Vegetables and Flowers

Hanzheng Chen

First Affiliated Hospital of Guangzhou Medical University

Rixu Liu

First Affiliated Hospital of Guangzhou Medical University

Tingshuai Zhang

First Affiliated Hospital of Guangzhou Medical University

Yi Chen

Columbia University

Dongyang Qian

First Affiliated Hospital of Guangzhou Medical University

Weixian Su

First Affiliated Hospital of Guangzhou Medical University

Yushu Chen

Nanjing Forestry University

Cooperative Institutions

Guangzhou Medical University

First Affiliated Hospital of Guangzhou Medical University

Key Laboratory of Guangdong Province

Southern Medical University

Nanjing Medical University

Chinese Academy of Sciences

Capital Medical University

University of Chinese Academy of Sciences

Nanchang University

Affiliated Hospital of Southwest Medical University

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[A prospective comparative study on effectiveness of single versus continuous adductor canal block combined with local infiltration anesthesia in unicompartmental knee arthroplasty].

PubMed (2024)

Dianqi Liu Wenbin Huang Wenrun Zhai Yingfen Li Dongfeng Chen

To compare the early analgesic effects and the impact on knee joint function recovery after unicompartmental knee arthroplasty (UKA) between single adductor canal block (SACB) and continuous adductor canal block (CACB) combined with local infiltration anesthesia (LIA) using a prospective study.

Adductor canal

Unicompartmental knee arthroplasty

Infiltration (HVAC)

Adductor muscles

10.7507/1002-1892.202312103

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Experimental study of three-dimensional co-culture system promoting dedifferentiated chondrocyte redifferentiation

Chin J Joint Surg(Electronic Edition) (2019)

Tingshuai Zhang Jianyu Zou Hanzheng Chen Ri-Xu Liu Shicong Zheng

Objective To investigate the effect of alginate hydrogel 3D co-culture on redifferentiation of dedifferentiated chondrocytes. Methods The first generation (P1) chondrocytes and the fourth generation (P4) chondrocytes were co-cultured with ATDC5 at the ratio of 3: 1, and wee named as AP1 and AP4 respectively. Another 3D culture system consisting of single P1, P4 and ATDC5 was established, and was named as P1, P4 and ATDC, respectively. All the groups were cultured in the chondrocyte-inducing medium with the supply of TGF-β3. After 28 days, the expression of collagen typeⅠ (ColⅠ), collagen typeⅡ (ColⅡ) and aggrecan (Acan) genes was assayed by Q-PCR. Histological staining was performed. The cartilage-related genes (Col Ⅱ and Acan) of each group were compared. The results were analyzed by Bonferroni test. Results The expression of ColⅡ and Acan in AP4 group was significantly up-regulated compared to P1 and P4 groups (ColⅡ: F=38.41, P<0.01, Acan: F=5, P<0.01 ), and the deposition of relative protein products could also be clearly observed on histological staining. Conclusion The 3D co-culture system can make the dedifferentiated chondrocytes regain their unique phenotype, the mechanism of which involves cellular factors and the cell-cell connection. Key words: Transforming growth factor beta3; Hydrogel; Collagen type Ⅰ; Collagen type Ⅱ; Biocompatible materials; Chondrocyte; Dedifferentiation

Aggrecan

Type II collagen

Type I collagen

10.3877/cma.j.issn.1674-134x.2019.06.008

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Gait analysis in knee diseases

Chin J Joint Surg(Electronic Edition) (2018)

Rixu Liu Yi Chen Yu‐Shu Cheng Jianyu Zou Shicong Zheng

Gait analysis is a well-established tool for the quantitative assessment of gait disturbances providing functional diagnosis, assessment for treatment planning, and monitoring of disease progress.The aims of gait analysis can be divided into three different categories: to characterize the gait pattern of a certain population, to classify and categorize the functional severity of a certain population and to evaluate the effect of a given intervention. This article briefly introduced the latest progresses of gait analysis in knee diseases including knee arthritis, anterior cruciate ligament injury and reconstruction, meniscus injury repair and so on. Key words: Knee, disease; Gait analysis; Meniscus; Anterior cruciate ligament; Osteoarthritis

Meniscus

10.3877/cma.j.issn.1674-134x.2018.06.012

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Gender differences in knee kinematics of Chinese during running

Chin J Joint Surg(Electronic Edition) (2019)

Rixu Liu Jianyu Zou Shicong Zheng Dongyang Qian Yushu Chen

Objective To investigate the kinematics of Chinese normal knee of running and compare gender differences. Methods Knee six-degree of freedom (6-DOF) kinematics of running were performed on 40 healthy adults (22 males and 18 females). Statistical analysis was performed using SPSS v16.0. The gender differences of kinematics were compared by one-way ANOVA. Results There were gender differences in kinematics of running in normal people. Gender differences mainly occured in the adduction/abduction angle. The average abduction angle of female was greater than that of male (F=5.563, P 0.05). Conclusions During running, the kinematics of knee is related to gender, and kinematic differences mainly exist in both frontal and transverse planes. The kinematics parameters of running in different genders provided in this study can provide valuable information for injury prevention, disease treatment and rehabilitation. Key words: Gait; Biomechanical phenomena; Knee; Running; Health; Sex

Knee flexion

Biomechanics

Statistical Analysis

10.3877/cma.j.issn.1674-134x.2019.05.008

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Investigation of normal knees kinematics in walking and running at different speeds using a portable motion analysis system

Sports Biomechanics (2021)

Rixu Liu Dongyang Qian Yushu Chen Jianyu Zou Shicong Zheng

Walking and running at different speeds are common in daily life. This study investigated 6 degrees of freedom (DOF) kinematics of normal knees of Chinese during walking and running. Forty healthy participants were investigated in 4 conditions: comfortable walking, normal walking, slow running and ordinary running. The range of motion (ROM) and peak values in 6 DOF kinematics were analysed. As the speed increased, a general increase in flexion, lateral and proximal translations occurred. Significant increases of ROM in flexion/extension, axial rotation and medial/lateral translations were observed. The ROM of adduction/abduction, anterior/posterior and proximal/distal translations were greatest during normal walking. The maximum and minimum flexion/extension, maximum internal rotation and tibial lateral translations increased with the increase of speed. The maximum and minimum tibial proximal translations in running were found being greater than walking. A phenomenon between walking and running was observed: both tibial proximal/distal and medial/lateral translations increased when changed from walking to running. Non-linear transition exists in 6 DOF kinematics during walking to running. Discoveries in this study may have potential clinical values to serve as references of normal walking and running in the management of knee injury and knee rehabilitation.

Knee flexion

External rotation

Internal Rotation

Motion analysis

10.1080/14763141.2020.1864015

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Application of co-culture system in articular cartilage tissue engineering

Chin J Joint Surg(Electronic Edition) (2018)

Jianyu Zou Ri-Xu Liu Shicong Zheng Yongchang Yao

Cartilage tissue engineering appears as an important strategy for repairing articular cartilage. However, it faces the problems of insufficient number of chondrocytes, dedifferentiation in vitro, and difficulty in controlling the direction of differentiation. The co-culture system provides a new solution to these problems. A series of studies have reported that the co-culture system can prevent the dedifferentiation of chondrocytes cultured in vitro, promote the re-differentiation of dedifferentiated chondrocytes, and facilitate the chondrogenic differentiation of stem cells in the system as well as inhibit the process of hypertrophy. This review summarized the literatures about the application of co-culture system in cartilage tissue engineering, discussed the role of co-culture system in cartilage tissue engineering, and explored the underlying mechanism. Key words: Coculture techniques; Chondrocytes; Tissue engineering

Chondrogenesis

10.3877/cma.j.issn.1674-134x.2018.06.017

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Enhanced chondrogenesis in a coculture system with genetically manipulated dedifferentiated chondrocytes and ATDC5 cells

Biotechnology and Bioengineering (2020)

Yongchang Yao Tingshuai Zhang Hanzheng Chen Shicong Zheng Yi Chen

Abstract Articular cartilage repair after injury is a great challenge worldwide due to its nerveless and avascular features. Tissue engineering is proposed as a promising alternative for cartilage regeneration. In this study, an adenoviral vector carrying the transforming growth factor‐β3 (TGF‐β3) gene was constructed and introduced into dedifferentiated chondrocytes, which were then cocultured with ATDC5 cells in an alginate hydrogel system. The results showed that the experimental groups exhibited better cell viability and higher levels of cartilage‐related genes than the control groups. In this coculture system, the chondrogenic differentiation of ATDC5 cells was effectively induced by TGF‐β3 and other latent cytokines that were produced by the transfected chondrocytes. Thus, this method can avoid the degradation of exogenous TGF‐β3, and it can protect ATDC5 cells during virus transfection to maintain cell viability and chondrogenic differentiation capability. Taken together, this study provides fresh insights for applying this genetically manipulated coculture system to cartilage repair in the future.

Chondrogenesis

Articular cartilage repair

Viability assay

SOX9

10.1002/bit.27482

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Citations (6)

Effect of passage number of genetically modified TGF-β3 expressing primary chondrocytes on the chondrogenesis of ATDC5 cells in a 3D coculture system

Biomedical Materials (2022)

Ke Chen Hanzheng Chen Hui Gao Wei Zhou Shicong Zheng

Abstract Due to the lack of blood vessels, nerves and lymphatics, articular cartilage is difficult to repair once damaged. Tissue engineering is considered to be a potential strategy for cartilage regeneration. Successful tissue engineering strategies depend on the effective combination of biomaterials, seed cells and biological factors. In our previous study, a genetically modified coculture system with chondrocytes and ATDC5 cells in an alginate hydrogel has exhibited a superior ability to enhance chondrogenesis. In this study, we further evaluated the influence of chondrocytes at various passages on chondrogenesis in the coculture system. The results demonstrated that transfection efficiency was hardly influenced by the passage number of chondrocytes. The coculture system with passage 5 (P5) chondrocytes had a better effect on chondrogenesis of ATDC5 cells, while chondrocytes in this coculture system presented higher levels of dedifferentiation than other groups with P1 or P3 chondrocytes. Therefore, P5 chondrocytes were shown to be more suitable for the coculture system, as they accumulated in sufficient cell numbers with more passages and had a higher level of dedifferentiation, which was prone to form a favorable niche for chondrogenesis of ATDC5 cells. This study may provide fresh insights for future cartilage tissue engineering strategies with a combination of a coculture system and advanced biomaterials.

Chondrogenesis

Articular cartilage repair

10.1088/1748-605x/ac489e

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Recent Research on Chondrocyte Dedifferentiation and Insights for Regenerative Medicine

Biotechnology and Bioengineering (2024)

Weixian Su Yuxue Nie Shicong Zheng Yongchang Yao

ABSTRACT Chondrocytes maintain the balance of the extracellular matrix by synthesizing glycoproteins, collagen, proteoglycans and hyaluronic acid. Chondrocyte dedifferentiation refers to a process in which chondrocytes lose their mature differentiated phenotype and transform into a fibroblast‐like morphology with fewer differentiated stages and inferior function under external stimulation. The important mechanism of homeostasis loss in osteoarthritis (OA) is a change in the chondrocyte phenotype. The dedifferentiation markers of chondrocytes are upregulated in OA, and the pathogenic factors related to OA have also been shown to enhance chondrocyte dedifferentiation. In this review, we compile recent studies on chondrocyte dedifferentiation, with an emphasis on potential markers and the underlying mechanisms of dedifferentiation, as well as the current research progress in inhibiting dedifferentiation or achieving redifferentiation. A deep understanding of chondrocyte dedifferentiation would not only support the pathogenesis of OA theoretically but also provide insightful ideas for regenerative medicine to manipulate the functional phenotype of cells.

Regenerative Medicine

10.1002/bit.28915

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Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel

Biotechnology Letters (2024)

Yongchang Yao Ke Chen Qian Pan Hui Gao Weixian Su

Chondrogenesis

Viability assay

10.1007/s10529-024-03475-2

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