The paper presents the study on the effects of the plasma activated water (PAW) generated by transient spark discharge on the wheat ( Triticum aestivum L.) cultivated in vitro and in vivo. Water uptake and germination of seeds, growth parameters of seedlings and plants, as well as the content of photosynthetic pigments, soluble proteins and activity of antioxidant enzymes are reported and correlated with the concentrations of reactive oxygen and nitrogen species in the PAW. The PAW improves germination, early development of the seedlings, the content of photosynthetic pigments in the leaves and soluble protein content in the roots, and suppresses the activity of antioxidant enzymes. The results indicate that the PAW may effectively stimulate growth of the wheat seedlings and positively affect their metabolism especially in the soil with low nutrient content.

Biological pigment

Photosynthetic pigment

10.1002/ppap.201800131

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Effect of Plasma Activated Water, Hydrogen Peroxide, and Nitrates on Lettuce Growth and Its Physiological Parameters

Applied Sciences (2021)

Katarína Kučerová M. Henselová Ľudmila Slováková Michaela Bačovčinová Karol Hensel

Cold plasma generated by atmospheric pressure air discharge is a source of various gaseous reactive oxygen and nitrogen species (RONS). When the plasma is generated in a contact with water, the RONS dissolve into water, change its chemical composition, while producing so-called plasma activated water (PAW). The PAW has the potential to be effectively used in various agricultural applications, as the long lived liquid RONS (H2O2, NO2−, NO3−) may act like signaling molecules in plant metabolism or serve as nutrients. We studied the effect of the PAW on lettuce plants and compared it with the effect of H2O2 and/or NO3− solutions of various concentrations to assess their role in the PAW. The PAW was generated from tap water by DC driven self-pulsing transient spark discharge. Pre-grown lettuce plants were cultivated in pots with soil and irrigated with the PAW or solutions of H2O2 and/or NO3−. After 5 weeks the growth parameters, number and quality of leaves, fresh and dry weight of plants, photosynthetic pigment (chlorophyll a + b) content, photosynthetic rate, and activity of antioxidant enzymes (superoxide dismutase, SOD) were evaluated. Lettuce plants irrigated with the PAW in comparison with chemically equivalent solution of H2O2 and NO3− had similar dry weight; however, the PAW induced higher photosynthetic pigment content, higher photosynthetic rate, and lower activity of SOD. The NO3− mainly contributed to the increase of dry weight, photosynthetic pigment content, photosynthetic rate, and overall better appearance of plants. The H2O2 contributed to an increase of dry weight and induced SOD activity. In general, H2O2 and NO3− in proper concentrations can stimulate plant growth and affect their physiological properties.

Dry weight

10.3390/app11051985

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Zařízení pro úpravu rostlinných semen nízkoteplotním plazmatemza atmosférického tlaku

Mirko Černák Pavel Šťahel Dušan Kováčik Anna Zahoranová M. Henselová

Technicke řeseni se týka zařizeni pro upravu rostlinných semen nizkoteplotnim plazmatem za atmosferickeho tlaku, ktere využiva elektricky buzeneho dielektrickeho koplanarniho povrchoveho barieroveho výboje (DCSBD) pro upravu osiva.

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Comparison of tolerance of Brassica juncea and Vigna radiata to cadmium

Biologia Plantarum (2007)

Ekaterina A. Simonova M. Henselová E. Masarovičová Jana Kohanová

The effect of different cadmium concentrations (6-120 µM) on Hill reaction activity (HRA) of isolated chloroplasts, contents of chlorophylls (Chls) and carotenoids (Cars), and Cd uptake and accumulation in plant organs of Indian mustard (Brassica juncea L. cv. Vitasso) and mung bean [Vigna radiata (L.) Wilczek] were determined. The Cd stress inhibited photochemical activity of isolated chloroplasts of both species and in both tested developmental stages. On the basis of EC50 values, the mung bean showed a higher sensitivity to Cd treatment than Indian mustard. The higher sensitivity of both species was determined in the earlier than in the older developmental stage. The leaves of Cd-treated plants possessed lower contents of Chls and Cars in both species and the negative effect increased with Cd concentration. A difference between species was also found in Cd uptake and accumulation. In both species, Cd was accumulated more in roots than in shoots, with higher accumulation in Indian mustard than in mung bean.

Radiata

10.1007/s10535-007-0103-z

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Citations (54)