Mauro Grabiele

National University of Misiones

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Humberto Debat

National Agricultural Technology Institute

Patricia M. Aguilera

National University of Misiones

Daniel A. Ducasse

National Agricultural Technology Institute

Julio R. Daviña

Consejo Nacional de Investigaciones Científicas y Técnicas

Ana I. Honfi

Instituto de Biología Subtropical

Eduardo A. Moscone

Instituto Multidisciplinario de Biología Vegetal

Marisel A. Scaldaferro

Instituto Multidisciplinario de Biología Vegetal

Glaucilene Ferreira Catroli

University of California, San Diego

Cleusa Yoshiko Nagamachi

Universidade Federal do Pará

Pablo Suárez

Consejo Nacional de Investigaciones Científicas y Técnicas

Cooperative Institutions

Consejo Nacional de Investigaciones Científicas y Técnicas

National University of Misiones

Instituto de Biología Subtropical

Universidade Estadual Paulista (Unesp)

National University of the Northeast

Centro Científico Tecnológico - Nordeste

University of Ferrara

Instituto Multidisciplinario de Biología Vegetal

Helsinki University Hospital

University of Helsinki

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The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis

Figshare (2023)

Patricia M. Aguilera Humberto Debat María Lorena Castrillo Gustavo Ángel Bich Mauro Grabiele

Abstract Dry leaves and twigs of yerba mate are widely infusion-consumed in southern Southamerica. Endemic and adapted to the Atlantic Forest, its extensive full-sun monoculture links to diverse biotic (pest, pathogens) and abiotic stresses (solar radiation, drought), impacting its productivity, ecology and socioeconomic niche. We focused in comprehensively characterize the DNAJ gene family in yerba mate to predict its possible roles on development and diverse stress responses to further assist crop manage. Our results suggest that yerba mate DNAJ proteins account 140 diverse members of six structural types displaying potential variable roles in protein homeostasis control. We were able to classify them into 51 distinct orthology groups, in agreement to Arabidopsis, and performed translational genomics of function, localization, expression and stress responsiveness data. Genome mapping and expression analysis indicated that yerba mate DNAJ genes differ in expression, nucleotide composition, length and exon-intron structure. Intronless or few introns genes -linked to rapid stress response- accounted 85 DNAJs. Promoters of DNAJ genes harbored a 73.2% of cis-acting regulatory elements involved in response to diverse stresses, hormones and light, simultaneously. We hypothesize that yerba mate DNAJs assist to plant survival during multiple stresses linked to current dominant agroecosystem but promote its growth under shade.

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10.6084/m9.figshare.22785637

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ESTUDIOS CROMOSÓMICOS EN ORQUÍDEAS SUBTROPICALES DE ARGENTINA CON VALOR ORNAMENTAL

Agro Sur (2007)

Julio R. Daviña Mauro Grabiele Juan Carlos Cerutti Diego Hojsgaard Rubén Almada

Ornamental plant

10.4206/agrosur.2007.v35n2-28

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CARACTERIZACION MORFOLOGICA Y CROMOSOMICA DE COMMELINA BENGHALENSIS L. (COMMELINACEAE) DE ARGENTINA MORPHOLOGICAL AND CHROMOSOMAL CHARACTERIZATION OF COMMELINA BENGHALENSIS L. (COMMELINACEAE) FROM ARGENTINA

DOAJ (DOAJ: Directory of Open Access Journals) (2009)

Mauro Grabiele Ana I. Honfi Marina Grabiele Humberto Debat Julio R. Daviña

Commelina benghalensis is a small herb native to tropical Africa and Asia but introduced elsewhere. In America it was found growing at open fields, border of woods and cultivated areas (south USA, Mexico, West Indies, French Guiana, Brazil, Bolivia, Paraguay) as a weed or invasive plant and its difficult manage causes economic damages in diverse agroecosystems all over the world. Commelina benghalensis was found at a border of Misionesforest (Argentina, Misiones Province, Guarani Departament, El Soberbio, 27o17'54.5''S; 54o12'19''W) and classical cytogenetic techniques were applied in order to analyze its genetic system. Commelina benghalensis is a diploid with 2 n = 2x = 22 median size chromosomes (4.05 - 6.60 µm) and 55.04 µm/genome. Its karyotype, 8m + 10sm + 4st is unimodal (A 2 = 0.14 / R = 1.63) and slightly asymmetrical (A 1 = 0.44 / i = 34.75 / r>2 = 0.55) (3A Stebbins category). Chromosome pair No. 11 ( st) has a terminal macrosatellite in the short arm and presumably carry the active NOR. Microsporogenesis is normal and produces viable polen grains (>80%). Meiotic behaviour is regular . In PMC at diakinecis / metaphase I, 11 bivalents were observed, mainly rings (92.7%) with distal chiasmata (96.7%) and an average of chiasmata / bivalent = 1.90. The meiotic system in conjuntion with a particular breeding system promotes a high genetic homogeneity , and suggests that this species has coadapted features with adaptative advantages allowing an invader behaviour .

Commelinaceae

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Contents Vol. 141, 2013

Cytogenetic and Genome Research (2013)

Roberta Santos Guilherme Mirlene Cecília Soares Pinho Cernach T.E. Sfakianakis S.S. Takeno L.M.M. Nardozza

10.1159/000356038

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Exploring the Genes of Yerba Mate (Ilex paraguariensis A. St.-Hil.) by NGS and De Novo Transcriptome Assembly

PLoS ONE (2014)

Humberto Debat Mauro Grabiele Patricia M. Aguilera Rosana Bubillo Mónica Beatriz Otegui

Yerba mate (Ilex paraguariensis A. St.-Hil.) is an important subtropical tree crop cultivated on 326,000 ha in Argentina, Brazil and Paraguay, with a total yield production of more than 1,000,000 t. Yerba mate presents a strong limitation regarding sequence information. The NCBI GenBank lacks an EST database of yerba mate and depicts only 80 DNA sequences, mostly uncharacterized. In this scenario, in order to elucidate the yerba mate gene landscape by means of NGS, we explored and discovered a vast collection of I. paraguariensis transcripts. Total RNA from I. paraguariensis was sequenced by Illumina HiSeq-2000 obtaining 72,031,388 pair-end 100 bp sequences. High quality reads were de novo assembled into 44,907 transcripts encompassing 40 million bases with an estimated coverage of 180X. Multiple sequence analysis allowed us to predict that yerba mate contains ∼ 32,355 genes and 12,551 gene variants or isoforms. We identified and categorized members of more than 100 metabolic pathways. Overall, we have identified ∼ 1,000 putative transcription factors, genes involved in heat and oxidative stress, pathogen response, as well as disease resistance and hormone response. We have also identified, based in sequence homology searches, novel transcripts related to osmotic, drought, salinity and cold stress, senescence and early flowering. We have also pinpointed several members of the gene silencing pathway, and characterized the silencing effector Argonaute1. We predicted a diverse supply of putative microRNA precursors involved in developmental processes. We present here the first draft of the transcribed genomes of the yerba mate chloroplast and mitochondrion. The putative sequence and predicted structure of the caffeine synthase of yerba mate is presented. Moreover, we provide a collection of over 10,800 SSR accessible to the scientific community interested in yerba mate genetic improvement. This contribution broadly expands the limited knowledge of yerba mate genes, and is presented as the first genomic resource of this important crop.

10.1371/journal.pone.0109835

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Citations (26)

The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis

Rodriguésia (2023)

Patricia M. Aguilera Humberto Debat María Lorena Castrillo Gustavo Ángel Bich Mauro Grabiele

WRKY protein domain

Comparative Genomics

10.1590/2175-7860202374020

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Citations (1)

Front & Back Matter

Cytogenetic and Genome Research (2013)

Roberta Santos Guilherme Mirlene Cecília Soares Pinho Cernach T.E. Sfakianakis S.S. Takeno L.M.M. Nardozza

10.1159/000356392

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Comparative cytogenetics in Cyclopogon (Orchidaceae)

Biologia (2012)

Mauro Grabiele Juan C. Cerutti Diego Hojsgaard Rubén Almada Julio R. Daviña

A cytotaxonomical description of Cyclopogon (Spiranthinae, Orchidaceae) is carried out through a deep karyotype analysis of four species from NE Argentina. Distinctive karyotype parameters concerning the chromosomes number, morphology, size and symmetry and the genome size associate to each taxon. Cyclopogon calophyllus (2n = 2x =28; 18m + 10sm), C. congestus (2n = 2x = 32; 26m + 6sm), C. elatus (2n = 2x = 28; 18m + 10sm) and C. oliganthus (2n = 4x = 64; 40m + 24sm) possess symmetrical karyotypes (i-mean = 40.01–42.84; A 1 = 0.24–032; r>2 = 0.06–0.29) and excluding C. congestus (A 2 = 0.26; R = 2.62) unimodality is the rule (A 2 = 0.12–0.20; R = 1.73–1.92). Diploid taxa show a terminal macrosatellite in the m pair no. 2 (large arm) and share a comparable mean chromosome length (ca. 2.75 μm) and genome size (ca. 40 μm), superior to the tetraploid C. oliganthus (ca. 2 and 32 μm, respectively). The novel data added to preceding cytological, morphological and molecular approaches involving Cyclopogon and those related taxa of Spiranthinae largely based on x = 23 support the hypothesis that the unusual 2n and the karyotype morphology of Cyclopogon is an evolutionary advance within Spiranthinae with a basic reduction to x = 14 or 16 by chromosome fusions. A polyploid-dysploid series added to dibasic hybridization explain the extant 2n diversity though a paleopolyploid series on x = 7–8 is also possible.

Polyploid

Genome size

Orchidaceae

Morphology

10.2478/s11756-012-0127-5

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A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae)

Rodriguésia (2021)

Patricia M. Aguilera Humberto Debat Guillermo Seijo Mauro Grabiele

Abstract Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as a distinctive ingredient, and popularly known as “locoto” or “rocoto”. This taxon is also an outstanding source of resistance to biotic and abiotic stresses as well as other valuable fruit traits for breeding of the worldwidely cultivated C. annuum and related species. In this study, the chromosome complement of C. pubescens cv. “locoto” (2n = 24) was deeply characterized through a sequential combination of conventional and molecular cytogenetics approaches comprising: Ag-NOR staining, heterochromatic fluorescent C-DAPI, DAPI/AMD-CMA/DA bandings, fluorescence in situ hybridization (FISH) of Capsicum-derived probes of the 5S and 18S-25S rRNA genes and different regions of spacers of the ribosomal unit, as well as telomeric probe. The markers identified were systematically combined with morphological karyotype parameters - number, size, centromeres, satellites - to produce a physical map which allowed the identification of several landmarks in each individual chromosome. The reference chromosomal map of C. pubescens here presented is the most comprehensively developed in Capsicum so far. It is envisioned that this chromosomal map will serve as a reference framework for the upcoming sequencing projects and as starting point to assist future genetic mapping of important agronomic traits.

Capsicum chinense

Molecular cytogenetics

10.1590/2175-7860202172062

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Supplementary Material for: Chromosome Evolution in Dendropsophini (Amphibia, Anura, Hylinae)

Figshare (2013)

Pablo Suárez Darío Cardozo Diego Baldo Martín O. Pereyra Julián Faivovich

Dendropsophini is the most species-rich tribe within Hylidae with 234 described species. Although cytogenetic information is sparse, chromosome numbers and morphology have been considered as an important character system for systematic inferences in this group. Using a diversity of standard and molecular techniques, we describe the previously unknown karyotypes of the genera Xenohyla, Scarthyla and Sphaenorhynchus and provide new information on Dendropsophus and Lysapsus. Our results reveal significant karyotype diversity among Dendropsophini, with diploid chromosome numbers ranging from 2n = 22 in S. goinorum, 2n = 24 in Lysapsus, Scinax, Xenohyla, and almost all species of Sphaenorhynchus and Pseudis, 2n = 26 in S.carneus, 2n = 28 in P.cardosoi, to 2n = 30 in all known Dendropsophus species. Although nucleolar organizer regions (NORs) and C-banding patterns show a high degree of variability, NOR positions in 2n = 22, 24 and 28 karyotypes and C-banding patterns in Lysapsus and Pseudis are informative cytological markers. Interstitial telomeric sequences reveal a diploid number reduction from 24 to 22 in Scarthyla by a chromosome fusion event. The diploid number of X.truncata corroborates the character state of 2n = 30 as a synapomorphy of Dendropsophus.

10.6084/m9.figshare.5125576

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