The flexibility and versatility of self-complementing split fluorescent proteins (FPs) have enabled a wide range of applications. In particular, the FP1-10/11 split system contains a small fragment that facilitates efficient generation of endogenous-tagged cell lines and animals as well as signal amplification using tandem FP11 tags. To improve the FP1-10/11 toolbox we previously developed, here we used a combination of directed evolution and rational design approaches, resulting in two mNeonGreen (mNG)-based split FPs (mNG3A1-10/11 and mNG3K1-10/11) and one mClover-based split FP (CloGFP1-10/11). mNG3A1-10/11 and mNG3K1-10/11 not only enhanced the complementation efficiency at low expression levels, but also allowed us to demonstrate signal amplification using tandem mNG211 fragments in mammalian cells.
Increasing bystander cardiopulmonary resuscitation (CPR) among racial/ethnic minority groups and culturally underserved populations is a key strategy in improving health care disparities in out-of-hospital cardiac arrest.
Objective
To ascertain whether implementation of the Los Angeles Tiered Dispatch System (LA-TDS) was associated with improved performance of telecommunicator-assisted CPR (T-CPR) among 9-1-1 callers with limited English proficiency in the City of Los Angeles.
Design, Setting, and Participants
This cohort study compared emergency medical services–treated, nontraumatic out-of-hospital cardiac arrest calls using the Medical Priority Dispatch System (MPDS) from January 1 to March 31, 2014, with calls using LA-TDS from January 1 to March 31, 2015. Trained data abstractors evaluated all 9-1-1 audio recordings for the initiation of T-CPR and the elapsed time to predefined events. Data were analyzed between January and December 2017.
Main Outcomes and Measures
The primary outcome was the prevalence of T-CPR among 9-1-1 callers with limited English proficiency for field-confirmed nontraumatic cardiac arrests. Additional outcomes included T-CPR among callers with English proficiency and the elapsed time until key events in the call.
Results
Of the 1027 emergency medical services calls during the study periods, 597 met the inclusion criteria. A total of 289 calls (48%) were made using MPDS (263 callers with English proficiency, and 26 callers with limited English proficiency), and 308 calls (52%) were made using LA-TDS (273 callers with English proficiency, and 35 callers with limited English proficiency). No differences between MPDS and LA-TDS cohorts were found in age, sex, known comorbidities, arrest location (private vs public), or witnessed status. The prevalence of T-CPR among callers with limited English proficiency was significantly greater using LA-TDS (69%) vs MPDS (28%) (odds ratio [OR], 5.66; 95% CI, 1.79-17.85;P = .003). For callers with English proficiency, the prevalence of T-CPR improved from 55% using MPDS to 67% using LA-TDS (OR, 1.66; 95% CI, 1.15-2.41;P = .007). With LA-TDS, callers with limited English proficiency had a significant decrease in time to recognition of cardiac arrest (OR, 0.59; 95% CI, 0.41-0.84;P = .005) and dispatch of resources (OR, 0.71; 95% CI, 0.54-0.94;P = .02).
Conclusions and Relevance
The LA-TDS compared with MPDS was associated with increased performance of T-CPR for out-of-hospital cardiac arrests involving 9-1-1 callers with limited English proficiency. Further studies are needed in communities with a predominance of people with limited English proficiency to characterize bystander response, promote activation of the chain of survival, and clarify the precise elements of LA-TDS that can improve T-CPR performance.
Spectral imaging is a powerful technique used to simultaneously study multiple fluorophore labels with overlapping emissions. Here, we present a computational spectral imaging method, which uses sample spatial fluorescence information as a reconstruction constraint. Our method addresses both the under-sampling issue of compressive spectral imaging and the low throughput issue of scanning spectral imaging. With simulated and experimental data, we have demonstrated the reconstruction precision of our method in two and three-color imaging. We have experimentally validated this method for differentiating cellular structures labeled with two red-colored fluorescent proteins, tdTomato and mCherry, which have highly overlapping emission spectra. Our method has the advantage of totally free wavelength choice and can also be combined with conventional filter-based sequential multi-color imaging to further improve multiplexing capability.
Char sulfonic acid and ammonium polyphosphate were used to improve the flame retardancy of composites. A synergistic effect between char sulfonic acid and ammonium polyphosphate on the flame retardancy of epoxy resin and polyamide composites was proposed. The results showed that the optimal synergistic effects were achieved when the weight ratio of char sulfonic acid:ammonium polyphosphate was 1:3. Thermogravimetric analysis indicated that the flame retardants improved the amount of residue. The char sulfonic acid and ammonium polyphosphate additives greatly decreased the peak heat release rate and suppressed smoke according to cone calorimetry testing. Scanning electron microscope results showed that a compact and thick char layer was formed, which hindered the transfer of heat flow and combustible gases in the condensed phase. Thus, the combination of char sulfonic acid and ammonium polyphosphate had been proved to be a promising flame retardant system for epoxy resin and polyamide composites.
Abstract In response to diverse stimuli, tumor cells can undergo a process resembling the epithelial-mesenchymal transition (EMT) observed during development. During this transition, epithelial-like tumor cells acquire mesenchymal-like physiologic features, become more motile and invasive, and acquire insensitivity to many therapeutic agents. While the importance of this phenomenon to cancer treatment and patient outcomes is well known, considerable heterogeneity exists across experimental models of EMT, and diverse mechanisms have been proposed to explain its various associated malignant phenotypes. Consequently, the extent to which these processes are shared uniformly across instances of EMT, as well as their relative importance in treatment contexts, remains unclear. We have used RNAseq, ATACseq, and parallelized high-throughput cell viability screening to identify differences in gene expression levels, chromatin accessibility, and drug sensitivity that correlate with EMT in a series of cell line models. By contrasting these changes across different genetic backgrounds and mechanisms of EMT induction, we are able to identify sets of elements in the transcribed and noncoding genome that are characteristic of different modes of EMT induction. Then, by comparing these candidate sets with observed differences in acquired drug sensitivity in the respective models, we were able to define a set of genetic elements whose biologic activity is likely to influence the efficacy of these compounds. Finally, we used a series of targeted CRISPR-based knockout screens to assess the relative importance of these elements to the viability of isogenic epithelial-like and mesenchymal-like cells, alone and in the presence of multiple therapeutic drugs. Using this approach, we were able to validate known mechanisms of acquired drug resistance and identify new candidate effectors. Notably, we identify multiple candidate noncoding elements containing CTCF binding sites that appear to meaningfully influence cell viability in a drug-specific manner, potentially implicating genome conformational changes in acquired therapeutic resistance. Citation Format: Russell O. Bainer, Catherine Wilson, Marinella Callow, Siyu Feng, Michael Costa, Colin Watanabe, Oleg Mayba, Eva Lin, Scott Martin, Bob Yauch, Richard Bourgon, Christiaan Klijn. Functional coding and noncoding drivers of EMT-mediated acquired drug resistance [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 398.
Gallium oxide (Ga2O3) has attracted much attention for its ultra-wide band gap and superior optoelectronic properties in the field of ultraviolet (UV) photodetectors. However, Ga2O3-based photodetectors still face problems such as slow response speed, large dark current and high power consumption. In this work, we design an n-Ga2O3/p-GaN vertical heterojunction in a transverse device and use it to construct a high-performance UV photodetector. The GaN film directly connects to the electrodes, while the Ga2O3 nanowires cover the top surface of the GaN and do not contact with any electrode. Based on this unique heterostructure, the photodetector demonstrates a low dark current and fast photoresponse speed under 254 nm irradiation. On the other hand, the photodetector can be operated at 0 V bias due to asymmetric Schottky barriers caused by the difference in electrode size. The photoresponse, specific detectivity and photoresponse speed of the photodetector are calculated to be 44.98 mA W-1, 5.33 × 1011 Jones and 383 ms, respectively, under self-powered mode. The enhanced UV photoresponse and self-powered mechanism of the photodetector are discussed in detail via the schematic diagrams of energy band structure and carrier transport process. At last, a vivid image is obtained using the Ga2O3/GaN photodetector as an imaging pixel in the imaging system. This work provides an effective strategy to construct high-performance UV photodetectors toward optical imaging.
AMERICAN SOCIETY OF PLASTIC SURGEONS PLASTIC & RECONSTRUCTIVE SURGERY PRS GLOBAL OPEN ASPS EDUCATION NETWORK AMERICAN SOCIETY OF PLASTIC SURGEONS PLASTIC & RECONSTRUCTIVE SURGERY PRS GLOBAL OPEN ASPS EDUCATION NETWORK
Eliminating poverty, improving people's livelihood, and gradually achieving common prosperity are the essential requirements of socialism. After more than 40 years of reform and opening up policy, China has embarked on a path of poverty alleviation with Chinese characteristics. In precise poverty alleviation, cadres in villages have become an important force to tackle the problem, and contact channels. Through studying the actual situation of the implementation of the basic strategy of the Party Central Committee’s precise poverty alleviation in the concentrated and contiguous areas of Yanshan-Taihang Mountain, it is truly reflected in the successful examples of grassroots forces winning the battle against poverty under the strong leadership of party committees and governments at all levels. Finally, the research demonstrates the new era style of the vast number of poverty alleviation cadres stationed in the village taking up the role, dedicating to the grassroots, and making sacrifices on the battlefield of poverty alleviation.
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