Heiko Ziebell

Julius Kühn-Institut

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Yahya Z. A. Gaafar

Canadian Food Inspection Agency

Katja R. Richert‐Pöggeler

Julius Kühn-Institut

Sébastien Massart

Gembloux Agro-Bio Tech

Thierry Candresse

Biologie du Fruit et Pathologie

Arnaud G. Blouin

Agroscope

Pascal Gentit

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

Marcel Westenberg

Netherlands Food and Consumer Product Safety Authority

Kris De Jonghe

Instituut voor Landbouw en Visserijonderzoek

Denis Kutnjak

National Institute of Biology

Neil Boonham

Newcastle University

Cooperative Institutions

Julius Kühn-Institut

University of the Punjab

Gembloux Agro-Bio Tech

University of Liège

Institute for Sustainable Plant Protection

Agricultural Research Service

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

United States Department of Agriculture

Centre National de la Recherche Scientifique

University of Kassel

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Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene

Journal of General Virology (2009)

Heiko Ziebell John P. Carr

In tobacco and Nicotiana benthamiana, limited cross-protection against cucumber mosaic virus strain Fny (Fny-CMV) was provided by prior inoculation with a deletion mutant lacking the 2b silencing-suppressor protein gene (Fny-CMVΔ2b). Cross-protection by Fny-CMVΔ2b did not result from induction of systemic RNA silencing. We investigated whether protection occurs through induction of localized RNA silencing by using Arabidopsis thaliana plants harbouring mutations in genes encoding the dicer-like (DCL) endoribonucleases 2, 3 and 4 involved in antiviral silencing. In wild-type A. thaliana (Col-0) plants, Fny-CMVΔ2b was symptomless and cross-protected against Fny-CMV infection. Cross-protection by Fny-CMVΔ2b against Fny-CMV infection was not abolished in dcl2, dcl3 or dcl4 mutant plants and was strongest in dcl2/4 double mutants, although in these plants and in dcl4 mutants, Fny-CMVΔ2b replicated to high levels and induced strong symptoms. The results suggest that Fny-CMVΔ2b/Fny-CMV cross-protection is not completely dependent on RNA silencing and also involves competition between these viruses.

RNA Silencing

Dicer

10.1099/vir.0.012070-0

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Citations (41)

A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria

Virology Journal (2019)

Yahya Z. A. Gaafar Katja R. Richert‐Pöggeler A. Sieg‐Müller P. Lüddecke Kerstin Herz

A tenuivirus, referred to here as JKI 29327, was isolated from a black medic (Medicago lupulina) plant collected in Austria. The virus was mechanically transmitted to Nicotiana benthamiana, M. lupulina, M. sativa, Pisum sativum and Vicia faba. The complete genome was determined by high throughput sequencing. The genome of JKI 29327 consists of eight RNA segments closely related to those of melon chlorotic spot virus (MeCSV) isolate E11–018 from France. Since segments RNA 7 and 8 of JKI 29327 are shorter, its genome is slightly smaller (by 247 nts) than that of E11–018. Pairwise comparisons between the predicted virus proteins of JKI 29327 and their homologues in E11–018 showed aa identities ranging from 80.6 to 97.2%. Plants infected with E11–081 gave intermediate DAS-ELISA reactions with polyclonal antibodies to JKI 29327. Since JKI 29327 and E11–018 appear to be closely related both serologically and genetically, we propose to regard JKI 29327 as the black medic strain of MeCSV. To our knowledge, JKI 29327 represents the second tenuivirus identified from a dicotyledonous plant. Serological and molecular diagnostic methods were developed for future detection.

Melon

Strain (injury)

10.1186/s12985-019-1195-8

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Citations (8)

A cucumber mosaic virus mutant lacking the 2b counter-defence protein gene provides protection against wild-type strains

Journal of General Virology (2007)

Heiko Ziebell Tina Payne James O. Berry John A. Walsh John P. Carr

Several plant virus mutants, in which genes encoding silencing suppressor proteins have been deleted, are known to induce systemic or localized RNA silencing against themselves and other RNA molecules containing homologous sequences. Thus, it is thought that many cases of cross-protection, in which infection with a mild or asymptomatic virus mutant protects plants against challenge infection with closely related virulent viruses, can be explained by RNA silencing. We found that a cucumber mosaic virus (CMV) mutant of the subgroup IA strain Fny (Fny-CMVDelta2b), which cannot express the 2b silencing suppressor protein, cross-protects tobacco (Nicotiana tabacum) and Nicotiana benthamiana plants against disease induction by wild-type Fny-CMV. However, protection is most effective only if inoculation with Fny-CMVDelta2b and challenge inoculation with wild-type CMV occurs on the same leaf. Unexpectedly, Fny-CMVDelta2b also protected plants against infection with TC-CMV, a subgroup II strain that is not closely related to Fny-CMV. Additionally, in situ hybridization revealed that Fny-CMVDelta2b and Fny-CMV can co-exist in the same tissues but these tissues contain zones of Fny-CMVDelta2b-infected host cells from which Fny-CMV appears to be excluded. Taken together, it appears unlikely that cross-protection by Fny-CMVDelta2b occurs by induction of systemic RNA silencing against itself and homologous RNA sequences in wild-type CMV. It is more likely that protection occurs through either induction of very highly localized RNA silencing, or by competition between strains for host cells or resources.

RNA Silencing

10.1099/vir.0.83138-0

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Citations (65)

Tomato brown rugose fruit virus (ToBRFV)

Anne Wilstermann Heiko Ziebell

Das tomato brown rugose fruit virus (ToBRFV) ist ein seit 2018 in Deutschland auftretender potentieller Quarantaneschadorganismus. Aufgrund der leichten mechanischen Ubertragbarkeit kann dieses Virus einfach innerhalb von Pfl anzenbestanden sowie zwischen verschiedenen Betrieben/Anbauorten verschleppt werden. Befallene Pflanzen mussen vernichtet werden.

10.5073/20190607-160917

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Citations (12)

The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species

Virus Research (2013)

Jan Kreuze R. Koenig Joao De Souza Heinrich Josef Vetten G. Müller

Latent Virus

Potato virus Y

10.1016/j.virusres.2013.01.014

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Citations (30)

Einsatz multispektraler Drohnenfotos zur Detektion und Ertragsabschätzung von Nanovirusinfektionen (PNYDV) in Ackerbohne (Vicia faba L.)

62. Deutsche Pflanzenschutztagung : Gesunde Pflanzen in Verantwortung für unsere Welt ; 21. - 23. September 2021, -Kurzfassungen der Vorträge und Poster- (2021)

J. Seeger Thomas Astor Heiko Ziebell Christiane Then Herwart Böhm

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Validation of high-throughput sequencing technologies pipeline for virology

Heiko Ziebell

10.5281/zenodo.1464819

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The effects of cucumber mosaic virus 2b protein on methyl jasmonate induced transcriptional responses and plant-aphid interactions in Arabidopsis

John P. Carr Mathew G. Lewsey James H. Westwood Heiko Ziebell Dan MacLean

Jasmonate

Methyl jasmonate

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Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description

EPPO Bulletin (2022)

B. S. M. Lebas Ian P. Adams Maher Al Rwahnih Steve Baeyen Guillaume J. Bilodeau

Abstract High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories for performing HTS testing. This paper describes general and technical recommendations to guide laboratories through the complex process of preparing a laboratory for HTS tests within existing quality assurance systems. From nucleic acid extractions to data analysis and interpretation, all of the steps are covered to ensure reliable and reproducible results. These guidelines are relevant for the detection and identification of any plant pest (e.g. arthropods, bacteria, fungi, nematodes, invasive plants or weeds, protozoa, viroids, viruses), and from any type of matrix (e.g. pure microbial culture, plant tissue, soil, water), regardless of the HTS technology (e.g. amplicon sequencing, shotgun sequencing) and of the application (e.g. surveillance programme, phytosanitary certification, quarantine, import control). These guidelines are written in general terms to facilitate the adoption of HTS technologies in plant pest routine diagnostics and enable broader application in all plant health fields, including research. A glossary of relevant terms is provided among the Supplementary Material.

Glossary

Identification

Plant quarantine

Amplicon sequencing

10.1111/epp.12863

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Citations (37)

Plant Defence and Viral Interference

Springer eBooks (2016)

Heiko Ziebell

Viral Interference

RNA Silencing

10.1007/978-3-319-25489-0_5

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Citations (3)