Objective To study the differential diagnostic value of detecting of Angiopoietin-2(Ang-2) in malignant and benign ascites.Methods The concentrations of Ang-2 and CEA in synovia of abdominal cavity of patients with benign ascites(n=82) and malignant ascites(n=119) were measured by ELISA technique.Results The concentrations of Ang-2 and CEA in the malignant ascites group were significantly higher than those in the benign ascites group(P 0.01).The sensitivity,specificity,total accurate rate and negative predictive value of Ang-2 was 89.9%,96.3% and 92.5%,86.8% respectively,and the sensitivity,the negative predictive value and the total accurate rate was significantly higher than those of CEA(P 0.05).Coclusion Ang-2 in malignant ascites shows a certain histio-specificity.Ang-2 is an useful tumor marker in differential diagnosis of benign and malignant ascites.
The patterns of carbon flow in large rivers influenced by dams are still not well known. We hypothesized that spatial variation in fish assimilation of basal production sources would be affected by heterogeneity of landscape-scale hydrology within a watershed due to the impoundment. We used stable isotope analysis and Bayesian mixing models to estimate relative contributions of basal production sources to fish consumers in a tributary of the Three Gorges Reservoir (TGR) with remarkably heterogeneous habitats between the upper and lower reaches. Model estimates indicated that riparian C3 plants were the main basal food source irrespective of reaches or feeding groups. Autochthonous algae carbon also appeared to be important as secondary carbon sources. However, the major contributive aquatic algae in the upstream were benthic algae while planktonic algae were important in the downstream, and this difference was related to their distinct hydrological characteristics. The contribution from C4 plants was comparatively less important to all fish consumers. Our results highlighted the significance of the riparian C3 carbon source to fish consumers in the TGR tributary and that the difference in landscape-scale characteristics was not enough to influence the pattern, which may be attributed to abundant standing stock of the riparian plants as well as hydrologic regimes in this river.
// Yuan Rong 1 , Hao Chen 1 , Xue-Feng Zhou 2 , Chang-Qing Yin 1 , Bi-Cheng Wang 3 , Chun-Wei Peng 4 , Shao-Ping Liu 5 , Fu-Bing Wang 1 1 Department of Laboratory Medicine & Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuchang, Wuhan 430071, P.R. China 2 Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital of Wuhan University, Wuchang, Wuhan 430071, P.R. China 3 Department of Pathology, Zhongnan Hospital of Wuhan University, Wuchang, Wuhan 430071, P.R. China 4 Department of Oncology, Zhongnan Hospital of Wuhan University, Wuchang, Wuhan 430071, P.R. China 5 Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuchang, Wuhan 430071, P.R. China Correspondence to: Fu-Bing Wang, e-mail: wfb20042002@sina.com Keywords: hepatocellular carcinoma, migration, invasion, cell-SELEX, aptamer Received: May 12, 2015 Accepted: January 13, 2016 Published: January 23, 2016 ABSTRACT Hepatocellular carcinoma (HCC) is one of the most deadly human cancers due to its ability of invasion and metastasis. Thus, the approaches to identify potential compounds that inhibit invasion and metastasis of HCC are critical for treatment of this disease. In the present study, we used HCCLM9 cells with high metastatic potential and MHCC97L with low metastatic potential as a model system to study the molecular mechanisms of HCC metastasis. By applying cell- Systematic Evolution of Ligands by Exponential enrichment (SELEX) against living cells, we used HCCLM9 as target cells and MHCC97L cells as control to screen a group of HCC metastasis- and cell-specific DNA aptamers. One of selected aptamers, LY-1, could specifically bind to metastatic HCC with a dissociation constant (Kd) in nanomolar range. In vitro studies demonstrated that LY-1 can recognize and bind to membrane protein of metastatic HCC cells. Furthermore, QD605 labeled LY-1 aptamer could recognize HCC cells in both local liver cancer tissues and pulmonary metastatic sites in a xenograft model of HCC with pulmonary metastasis. Further biochemical and immunostaining studies showed that LY-1 could selectively bind to a subpopulation of more metastatic cells in HCCLM9 cells, which express more CK19 and vimentin. Finally, treatment of highly metastatic cells with LY-1 led to reduced migration and invasiveness of HCCLM9 cells in vitro and suppression of xenograft growth in vivo . Taken together, the present study demonstrated the tumor targeting and tumor suppressive effects of LY-1, which could be a promising molecular probe for metastatic HCC and a potential candidate of chemotherapy for metastatic HCC.
Objective To analyse the epidemiological features of typhoid paratyphoid. Methods Desriptive epidemiological method was used to analyse the data. Results From 2004 to 2007,a total of 245 cases were confirmed,in which 166 cases(67.75%)were in Junlian County with all average annual incidene rate of 1.24/105 (annual incidence rate ranged 0.55/105-2.4/105).The disease revealed no seasonal or sexual differences. About 84.90% cases aged 40 years old. Most cases were students,farmers,cadres or office workers,persons engaged in household daties or awaiting employment and children outside kindergarten(71.84%). Conclusion The epidemiological feature of typhoid in Yibin City from 2004 to 2007 showed that the disease concentrated in Junlian County and distribated in broad occupations.
PDF HTML阅读 XML下载 导出引用 引用提醒 5种鱼类标志对草鱼临界游泳速度的影响 DOI: 作者: 作者单位: 1. 西南大学 生命科学学院, 重庆 400715;2. 农业部长江中上游渔业资源环境科学观测实验站, 中国水产科学研究院 长江水产研究所, 湖北 武汉 430223 作者简介: 俞立雄(1991-),男,博士研究生,专业方向为渔业资源.E-mail:568264442@qq.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目（51579247）；科技部支撑计划项目（2015BAD13B02） Effect of five tagging methods on critical swimming speed of grass carp, Ctenopharyngodon idellus Author: Affiliation: 1. Faculty of Life Sciences, Southwest University, Chongqing 400715, China;2. Key Field Experimental Station for Scientific Observation of Fishery Resources and Environment of the Middle and Upper Reaches of the Yangtze River, Ministry of Agriculture; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:采用被动整合雷达标志法（PIT）、切鳍标记、荧光标记、超声波标志和T型标志这5种标志方法对草鱼（）进行标记后，测定其临界游泳速度，研究5种不同标记对草鱼游泳能力的影响。将试验草鱼按不同体长分为3组：15~18 cm，18~21 cm，21~25 cm，每个体长组均设置对照组，并分别进行以上5种鱼类标志，测量标志后草鱼的绝对临界游泳速度和相对临界游泳速度。采用SPSS17.0统计软件进行数据的分析比较。研究结果表明，随着体长增加，草鱼的绝对临界游泳速度增大，相对临界游泳速度减小；超声波标志对3个体长组草鱼的临界游泳速度均有极显著影响（<0.01），标志后草鱼绝对临界游泳速度分别下降18.72%、16.40%、23.15%，相对临界游泳速度分别下降18.95%、17.78%、21.86%；T型标志对15~18 cm体长组草鱼的临界游泳速度有极显著性影响（<0.01），标志后草鱼绝对临界游泳速度下降8.35%，相对临界游泳速度下降9.30%，对体长大于18 cm的草鱼无显著性影响；PIT标志、切鳍标记和荧光标记对这3个体长组草鱼的临界游泳速度均无显著性影响（>0.05）。 Abstract:This research assessed the effect of five tagging methods: namely, PIT marking, fin amputation marking, fluorescent marking, ultrasound marking and T-type marking, on the critical swimming speed of grass carp . Grass carp with a body length of 15-25 cm (standard length, SL) were divided into three size groups: short body length (15-18 cm), medium body length (18-21 cm) and long body length (21-25 cm). Critical swimming speed was measured for 144 fish in an annular flume with a water temperature of (16±1)℃. SPSS17.0 software was used for data processing. The results showed that the absolute critical swimming speed of the grass carp tended to increase, while the relative critical swimming speed tended to decrease along with an increasing body length. Ultrasonic tagging showed a significant impact (<0.01) on the swimming ability of the fish in all size groups (body length 15-25 cm). The absolute critical swimming speed of the grass carp in each of the three size groups (short, medium, large) decreased by 18.72%, 16.40% and 23.15%, respectively, after ultrasound tagging, and the relative critical swimming speed of the three groups decreased by 18.95%, 17.78% and 21.86%, respectively. T-type tagging also showed a significant impact (<0.01) on the swimming ability of grass carp in the short body length group: their absolute critical swimming speed decreased by 8.35%, whereas their relative critical swimming speed decreased by 9.30%. No significant impact of T-type tagging was detected on the critical swimming speed of grass carp with a medium or long body length (18-25 cm). Finally, no significant impact on swimming speed was detected for fish with a PIT tag, fin amputation marking or fluorescent tag. 参考文献 相似文献 引证文献
To explore the effective method of preventing cubitus varus deformity in nonoperative treatment of humeral supracondylar fracture in children.From May 1992 to December 2013,319 patients with hemeral supracondylar fracture in children were treated with manual reduction and external plaster fixation in extension position. There were 253 males and 66 females, aged from 15 months to 13 years old with an average of 6.7 years. Among the patients with humeral supracondylar fracture, extension type was in 284 cases and inflexion type was in 35 cases; 167 cases on the left and 152 cases on the right. Injury to treatment time was 1 hour to 7 days with a mean of 1.8 days. No included nerve injury and operative case in the patients.All patients were followed up from 3 months to 14 years with an average of 37.3 months. All fractures obtained bone healing and healed time was 6 to 8 weeks with an average of 6.9 weeks. No complications of serious cubitus varus deformity,vascular nerve injury or volkmann contracture were found. In 183 cases, the carrying angle was consistent with itself contralateral,ranged from 5° to 15°; in 105 cases, the carrying angle of uninjuryed side was decreased. During the reset, the carrying angle of 26 patients with ulnar deviation angle was for -5° to 0°; and 5 patients with radial deviation angle was for 15° to 18°, basically does not affect the appearance. The activities of all elbow joint were normal.External plaster fixation in extension position has some value to preventing cubitus varus deformity in nonoperative treatment of humeral supracondylar fracture in children and hope that more scholars to do further study.
Objective To investigate the correlation between expression of human telomerase reverse transcriptase (hTERT) mRNA in peripheral blood and postoperative recurrence and prognosis in the patients with advanced colorectal cancer (CRC).Methods The preoperative and 1 month postoperative quantitative expression of hTERT mRNA in peripheral blood in 85 patients with advanced CRC were detected with real-time fluorescence quantitative real-time fluorescence quantitative polymerase chain reaction (RTPCR).The quantitative expression of hTERT mRNA in peripheral blood in 30 normal controls were also detected.The postoperative prognosis was followed-up,and the quantitative expression of hTERT mRNA in peripheral blood in the patients with recurred CRC and the patients without recurred CRC were detected repeatedly.The factors that affected the preoperative hTERT mRNA expression were analyzed.The relationship was investigated between the hTERT mRNA expression levels and postoperative recurrence and prognosis in the patients with advanced CRC.Results The hTERT mRNA expression levels in peripheral blood in advanced CRC group were significantly higher than those in the normal controls [(6.22 ±5.85)2-△△Ct vs (0.45 ±0.27)2-△△Ct,(P ＜0.01)],and were significantly decreased at the time point of 1 month after the operation (P ＜ 0.01).The hTERT mRNA expression levels in the postoperative recurred CRC group were significantly higher than those in the 1 month postoperation group and no recurrence CRC group [(5.07 ± 2.87)2-△△Ct vs (1.83 ± 1.08)2-△△Ct,(2.15 ± 1.49)2-△△Ct,(P ＜0.01)].Univariate analysis revealed that preoperative high hTERT mRNA expression levels in peripheral blood were related to the differentiated degree of tumor,tumor size,lymph node metastasis ratio,and liver metastasis (P ＜ 0.05 or P ＜ 0.01).Multivariate logistic regression analysis revealed that differentiated degree of the tumor,lymph node metastasis,and liver metastasis were independent risk factors for the elevation of preoperative hTERT mRNA expression in the advanced CRC.Elevated preoperative hTERT mRNA expression was positively related to the postoperative recurrence rate,and was negatively related to postoperative survival rate.Conclusions Preoperative hTERT mRNA expression levels in peripheral blood were related to the important pathologic features in patients with advanced CRC,and might be a valuable prognostic factor.Postoperatively elevated hTERT mRNA expression in peripheral blood was related to recurrence of advanced CRC.
Key words:
Telomerase/genetics ; RNA, messenger/genetics ; Telomerase/blood ; Colorectal neoplasms/surgery ; Colorectal Neoplasms/blood ; Reverse transcriptase polymerase chain reaction ; Recurrence ; Prognosis
The Nu River Project comprised of 13-dam cascade is one of the biggest hydropower development projects yet conceived in China. However, hydropower development will inevitably result in the local ecological environment and biological material impact, particularly the effects on fish. Glyptothorax zainaensis is weak in swimming with suction cup and is extremely sensitive in the hydrological characteristics and water quality changes, so that it can be considered as a water quality monitor of indicator organism. Currently, with the need for conservation, fishery restoration and so on, there is a great demand for genetic information on Glyptothorax zainaensis. Fourty microsatellite loci were isolated, characterized and evaluated from Glyptothorax zainaensis. A total of 91 different alleles were observed in the 303 wild Glyptothorax zainaensis, and the percentage of polymorphic bands ranged from 41.8% to 66.7%, with an average of 90.1%. The genetic diversity value was significantly huge with the expected heterozygosity ranging from 0.578 to 0.706, the observed heterozygosity value ranging from 0.923 to 0.980 and PIC value ranging from 0.418 to 0.644, which showed that the wild Glyptothorax zainaensis population had a high level of genetic diversity. Analysis of molecular variance (AMOVA) and pairwise Fst showed a significant level of genetic differentiation (P
The complete mitogenome sequence of Glyptothorax laosensis was determined using long PCR reactions. The genome is 16 539 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of G. laosensis was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 31.3% A, 27.5% C, 25.9% T, 15.3% G, with a relatively lower level of G and a slight AT bias of 57.2%. The results of phylogenetic reconstruction showed G. laosensis is the sister group with G. trilineatus (Figure 1), and the Glyptothorax genus formed a monophyletic group.
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