Huan Du

China University of Petroleum, East China

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Gang Fan

Chengdu University of Traditional Chinese Medicine

Ruyan Zhang

Hubei Polytechnic University

Xiandeng Hou

Sichuan University

Tong Xu

Lanzhou University of Technology

Junbo Chen

Jilin Agricultural University

Zhiqing Zhang

China University of Petroleum, East China

Peng Yang

Analysis and Testing Centre

Xiufeng Wang

China University of Petroleum, East China

Ting Zhou

South China University of Technology

Tingting Kuang

Chengdu University of Traditional Chinese Medicine

Cooperative Institutions

Chengdu University of Traditional Chinese Medicine

Sichuan University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

China University of Petroleum, East China

Peking University

Capital Medical University

Guizhou University

Sun Yat-sen University

West China Hospital of Sichuan University

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Activation of catalytic DNAzyme by binding‐induced DNA displacement for homogeneous assay

Luminescence (2021)

Wei Fan Junbo Chen Huan Du Changjia Hu Peng Yang

Abstract The sensitive assays for protein, especially for the DNA‐based assay, are often dependent on the amplification procedure with assistance of enzyme. Compared with a protein enzyme, deoxyribozyme (DNAzyme) exhibits similar catalytic activity and specificity and better flexibility. In this work, we streamlined the binding induced DNA displacement (BINDD) strategy for the activation of DNAzyme cleavage. Since the intrinsic element of DNAzyme is the nucleic acids, it is easy to join the BINDD by hybridization with an affinity probe. The activity of DNAzyme was initiated by the BINDD reaction mediated by the recognition affinity probe with target proteins. Upon DNAzyme release, it was able to catalyze and cleave the predesigned substrates, generating the enhanced fluorescence signal indicating the protein concentration. Such a constructed homogeneous assay is available and effective in human serum when it was used for detection of platelet derived growth factor‐BB (PDGF‐BB) and prostate specific antigen (PSA), with detection limits of 100 pM and 200 pM, respectively.

Deoxyribozyme

Cleave

Cleavage (geology)

10.1002/bio.4092

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Citations (2)

Design, Synthesis and Biological Activities of Novel Quinazolinone Derivatives Bearing 4-Phenyl-5-thioxo-1,2,4-triazole Mannich Bases

Chinese Journal of Organic Chemistry (2016)

Boren Yan Xinyang Lü Huan Du Xiaoping Bao

Quinazolinone

Triazole

1,2,4-Triazole

10.6023/cjoc201506026

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Citations (11)

[Determination of six compounds in Berberidis Cortex and comparative study of its different species].

PubMed (2019)

Qi Li Huan Du Huan-Song Wen Tong Xu Yu-Jie Wang

In order to clarify the characteristic components of Berberidis Cortex,the preparative liquid chromatography and spectral analysis methods were used to separate and identify the unknown components in the water extract of Berberidis Cortex. Two compounds were isolated and identified as bufotenidine and ferulic acid 4-O-β-D-glucopyranoside. They were both isolated for the first time from Berberidis Cortex and Berberis. In addition,an HPLC method was successfully established for simultaneously determination of six compounds in Berberidis Cortex,and chemometric methods were used to study the chemical differences among three main species of Berberidis Cortex. The results suggested that jatrorrhizine and bufotenidine are the main difference compounds among the three species.Compared with B. kansuensis and B. diaphana,B. vernae contains significantly more jatrorrhizine(P<0. 01),and the content of bufotenidine in B. vernae was significantly higher than that in B. kansuensis(P<0. 05). Considering these results,further research is necessary to reveal the pharmacological activities of bufotenidine and the pharmacodynamic differences between the three species. The results could provide a reference for quality control,the basic research on effective substances,and development of Berberidis Cortex.

Jatrorrhizine

10.19540/j.cnki.cjcmm.20181226.014

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Citations (9)

Expanding DNA nanomachine functionality through binding-induced DNA output for application in clinical diagnosis

Chemical Communications (2019)

Huan Du Peng Yang Xin Hou Rongxing Zhou Xiandeng Hou

Herein, we describe two homogeneous conversion systems that can convert protein recognition into the release of predesigned output DNA for the activation of DNA nanomachines. With high conversion efficiency, this strategy allows us to initiate DNA nanomachines to respond to any proteins, opening up the possibility of applying DNA nanomachines in clinical practice.

10.1039/c9cc01228a

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Citations (13)

Response mechanism of ♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus under low-temperature and waterless stresses using TMT proteomic analysis

PROTOPLASMA (2021)

Xiuping Fan Qiaoyu Guo Jiasheng Zhang Huan Du Xiaoming Qin

♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus, a hybrid grouper created from artificial breeding, has been widely developed over the past decades. However, the study focusing on lukewarm high-protein-content fish species using advanced techniques has rarely been reported. In this work, the TMT (tandem mass tag)-assisted technique was employed to explore its differentially expressed proteins and response mechanisms under low-temperature dormant and waterless stresses. Our findings suggest that 162 and 258 differentially expressed proteins were identified under low-temperature dormant and waterless stresses, respectively. The waterless preservation treatment further identifies 93 differentially expressed proteins. The identified proteins are categorized and found to participate in lipid metabolism, glycometabolism, oxidative stress, immune response, protein and amino acid metabolism, signal transduction, and other functions. Accordingly, the factors that affect the response mechanisms are highlighted to provide new evidences at protein level.

Epinephelus

10.1007/s00709-021-01654-w

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Citations (4)

Accelerating DNA nanomotor by branched DNAzyme for ultrasensitive optical detection of thrombin

Microchemical Journal (2018)

Huan Du Peng Yang Xin Hou Xiandeng Hou Junbo Chen

Nanodevice

Deoxyribozyme

10.1016/j.microc.2018.03.004

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Citations (13)

Endothelial function and plasma matrix metalloproteinase‐2 levels and their association with the size and elastic properties of the ascending aorta in first‐degree relatives of bicuspid aortic valve patients

Echocardiography (2020)

Li Yang Yi‐Bin Wang Ying Zhang Shuai Zhao Peng Jin

Abstract Purpose To explore endothelial function and plasma matrix metalloproteinase‐2 levels and their association with the size and elastic properties of the ascending aorta in the first‐degree relatives (FDRs) with tricuspid aortic valve (TAV) of individuals with bicuspid aortic valve (BAV). Methods Twenty‐six patients with BAV without significant valvular dysfunction, 35 FDRs with TAV, and 29 matched healthy controls were analyzed. Two‐dimensional echocardiography and high‐resolution ultrasound were applied to evaluate the size and elasticity of the ascending aorta and the flow‐mediated vasodilation (FMD) of the brachial artery in response to hyperemia. Results The aortic diameter was larger in the BAV patients than in the FDRs and controls, and the aortic elastic properties showed larger decreases in the BAV patients. The FDRs had a larger aortic diameter and more impaired elastic properties than the controls. The BAV patients had a significantly lower FMD than the FDRs and controls ( P < .001), while the FMD was lower in the FDRs than in the controls ( P < .001). The plasma MMP‐2 levels were significantly higher in the BAV patients than in the FDRs ( P = .001) and controls ( P < .001). Additionally, the FDRs had higher plasma MMP‐2 levels than the controls ( P < .001). Pearson correlation analysis revealed that the aortic diameter, distensibility, stiffness index, and strain were significantly associated with FMD and plasma MMP‐2 levels. Conclusion Aortic enlargement and impaired elasticity in the FDRs with TAV were associated with impaired endothelial function and increased plasma MMP‐2 concentrations.

Endothelial Dysfunction

10.1111/echo.14594

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Citations (1)

Effect of Different Processing Methods on the Chemical Constituents of Scrophulariae Radix as Revealed by 2D NMR-Based Metabolomics

Molecules (2022)

Xiaohui Duan Mina Zhang Huan Du Xiu Gu Caihong Bai

Scrophulariae Radix (SR) is one of the oldest and most frequently used Chinese herbs for oriental medicine in China. Before clinical use, the SR should be processed using different methods after harvest, such as steaming, “sweating”, and traditional fire-drying. In order to investigate the difference in chemical constituents using different processing methods, the two-dimensional (2D) 1H-13C heteronuclear single quantum correlation (1H-13C HSQC)-based metabolomics approach was applied to extensively characterize the difference in the chemical components in the extracts of SR processed using different processing methods. In total, 20 compounds were identified as potential chemical markers that changed significantly with different steaming durations. Seven compounds can be used as potential chemical markers to differentiate processing by sweating, hot-air drying, and steaming for 4 h. These findings could elucidate the change of chemical constituents of the processed SR and provide a guide for the processing. In addition, our protocol may represent a general approach to characterizing chemical compounds of traditional Chinese medicine (TCM) and therefore might be considered as a promising approach to exploring the scientific basis of traditional processing of TCM.

Radix (gastropod)

10.3390/molecules27154687

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Citations (4)

Spatially Constrained DNA Nanomachines To Accelerate Kinetics in Response to External Input: Design and Bioanalysis

Analytical Chemistry (2020)

Rongxing Zhou Changjia Hu Yanwen Jin Jie Zhang Huan Du

Cells take advantage of the spatial organization to accelerate the reaction kinetics of diverse components within a crowded intracellular environment. Inspired by this, we hereby designed a principle of spatial constraint to overcome limitations of response kinetics in DNAzyme-powered DNA nanomachines. First, we proposed the type-1 of spatially constrained DNA nanomachines (scDN-1) by co-localizing the aptamer probe and power unit (DNAzyme), allowing the DNA nanomachines to accomplish faster cyclic cleavage of DNAzyme as intramolecular reactions. To expand the scDN into the clinical practice, Type 2 spatially constrained DNA nanomachines (scDN-2) with constrained antibody probes were then constructed through Holliday junction assembly, which increased the effective local concentration to obtain the improved kinetics. With an accelerated response kinetics, this design principle allows DNA nanomachines to accomplish the response to tumor markers in real patients’ samples within 30 min, significantly broadening the bioanalytical applications of DNA nanomachines to clinical practice.

Bioanalysis

Deoxyribozyme

Aptamer

Nanodevice

DNA nanotechnology

10.1021/acs.analchem.0c00802

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Citations (24)

Sensitive detection of trace 4-methylimidazole utilizing a derivatization reaction-based ratiometric surface-enhanced Raman scattering platform

Talanta (2021)

Lichen Gou Xiaoliang Zeng Huan Du Ling Li Yunfei Tian

Imidazole

Trace Amounts

10.1016/j.talanta.2021.122925

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Citations (7)