Kurbatova Ea

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

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Egorova Nb

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

Semenova Ib

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

Efremova Vn

Semenov Bf

Chumakov Institute of Poliomyelitis and Viral Encephalitides

НК

Н. К. Ахматова

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

Gruber Im

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

Kiselevskiĭ Mv

Kuz'mina La

Ministry of Health of the Russian Federation

Vorob'ev Ds

RÈ

Romanenko Èe

Cooperative Institutions

Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences

Russian Academy of Sciences

Academy of Medical Sciences

Ministry of Health of the Russian Federation

Gamalei Institute of Epidemiology and Microbiology

Sechenov University

D.I. Ivanovsky Institute of Virology Russian Academy of Medical Sciences

Institute of Pharmacology Russian Academy of Medical Sciences

Lomonosov Moscow State University

Center for Biologics Evaluation and Research

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[Protective effect of the aerosol immunization of mice with the polycomponent vaccine Immunovac VP-4 after the challenge of the animals with Klebsiella pneumoniae virulent strain].

Zhurnal mikrobiologii epidemiologii i immunobiologii (2006)

Solov'ev Kg Kurbatova Ea Egorova Nb Uspenskaia Sn Tret'iakova Av

Used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with K. pneumoniae virulent strain K16. Irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized animals. After intervals between immunizations were increased to 3 days the protective effect of aerosol immumization was obtained (the survival rate was 65-80 % and considerably differed from that of the controls). The protective effect of aerosol immunization thus obtained was comparable with the effectiveness immunization made in a single subcutaneous injection. Aerosol immunization resulted in low antibody titers to the antigens contained in the vaccine, while after a single subcutaneous injection high antibody titers to Klebsiella and Proteus antigens were detected. The antigen-stimulated blast transformation of spleen lymphocytes in mice subjected to aerosol immunizations in 5 exposures was high. After subcutaneous immunization significant changes in such characteristics were detected on day 15. The data thus obtained were indicative of good prospects in the development Immunovac VP-4 as the medicinal form intended for use in aerosols.

Antibody titer

Subcutaneous injection

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[Protective activity of a cell-free Klebsiella vaccine in relation to different Klebsiella pneumoniae serovars].

PubMed (1984)

Kurbatova Ea Egorova Nb Kiseleva Bs

The capacity of dried Klebsiella cell-free vaccine, obtained from strain No. 204 by the disintegration of microbial cells with hydroxylamine, for protecting mice from Klebsiella septic infection caused by the homologous serovar and 9 heterologous serovars of K. pneumoniae was studied. The newly developed preparation was found capable of stimulating immunity not only to the homologous K. pneumoniae serovar, but also to other K. pneumoniae heterologous serovars: K1, K9, K11, K16, K20, K61. The protective capacity of the preparation with respect to these serovars was not inferior to that of the vaccines prepared by the same method from the corresponding homologous strains. The capacity of the vaccine to protect mice from Klebsiella sepsis was manifested irrespective of the virulence of the strains used for challenge.

Heterologous

Klebsiella

Strain (injury)

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Protective activity of secreted proteins of Streptococcus pneumoniae and Klebsiella pneumoniae

Zhurnal mikrobiologii epidemiologii i immunobiologii (2008)

Trishin Av Donenko Fv Kurbatova Ea Voiushin Ke Kiselevskiĭ Mv

Protective efficacy of secreted proteins of Streptococcus pneumoniae and Klebsiella pneumoniae cultivated on cardiocerebral broth and semisynthetic growth medium respectively was studied in vivo. Fraction with molecular weight 30 - 50 kDa obtained by the method of membrane fractionation had high protective efficacy. Two-dose immunization of mice with this fraction provided 80 - 100% protection from infection by homologous strains of S. pneumoniae and K. pneumoniae. Cross-protective activity of the fraction was revealed when infecting immunized mice by different K-types of K. pneumoniae. Blood sera of mice immunized with 30 - 50 kDa fraction possessed preventive features protecting from infection 90% of animals while 100% of death in the control group. It was determined that protective efficacy of the mentioned fraction was determined by protein-containing antigens because proteolytic disruption of the protein component resulted in loss of protective properties of the preparation.

Pneumococcal infections

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[Strain differences of intra-species immunogenic activity of Streptococcus pneumoniae antigen components].

PubMed (2014)

Kurbatova Ea Vorob'ev Ds Egorova Nb Baturo Ap Romanenko Èe

Study intra-species immunogenic activity of antigenic protein-polysaccharide components of S. pneumoniae.Antigenic components of serotype 3, 6A, 6B, 14, 10A, 18A, 19A, 19F, 23F and unencapsulated S. pneumoniae strains were obtained by water extraction method. Synthetic hexasaccharide--corresponding to the structure of S. pneumoniae serotype 14 capsule polysaccharide repeated unit chain fragment was used as a reference preparation. Molecular mass of antigenic components was determined in SDS-electrophoresis. Antibody titers in blood sera of immunized mice were evaluated by solid-phase EIA method. Protective activity of preparations was studied in mice after 2 immunizations with consequent infection by virulent S. pneumoniae serotype 3 and 6B strains.Preparations from serotype 6A, 6B, 14, 19A, 19F, 23F strains in reaction with anti-microbial sera were characterized by cross serologic activity (IgG titers of 1200 - 12 800). The lowest serologic activity was detected in S. pneumoniae serotype 3 and unencapsulated strain preparations. Conjugate of synthetic hexasaccharide and bovine serum albumin interacted only with homologous antimicrobial sera up to titers of 600 +/- 89.4 and did not react with sera against serotypes 19A and 19E Cross serologic activity of preparations is probably determined by the presence of protein fractions that were detected in SDS-electrophoresis. This is confirmed by high intra-species cross protective activity of preparations from serotype 6B and 10 A strains that protect 90 - 100% of mice from infection by heterologous S. pneumoniae strains.Use of strains with cross antigenic and protective activity for production of immunogenic protein-containing fractions with the aim of enchanting and broadening specter of protective activity of vaccine preparations that are constructed based on capsule polysaccharides of S. pneumoniae is appropriate.

Heterologous

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[Influence of dendrite cells, generated with the use of immunomodulators of microbial origin, on the proliferative and cytotoxic activity of lymphocytes].

Zhurnal mikrobiologii epidemiologii i immunobiologii (2005)

Н. К. Ахматова Lebedinskaia Ov Kiselevskiĭ Mv Makashin Ai Semenova Ib

The influence of ripe dendrite cells (DC) on the proliferative activity of mononuclear leukocytes and the cytotoxic activity of lymphocytes of syngenic mice was studied. As inducers of DC ripening, the combination of antigenic components incorporated into the vaccine lmmunovac Bh-4, (Klebsiella pneumoniae, Proteus vulgaris, Escherichia coli, Staphylococcus aureus), as well as K. pneumoniae LPS and TNF-alpha, were used. This study demonstrated that DC activated with these preparations enhanced the proliferative activity of mononuclear leukocytes, the activity of Bh-4 being higher than that of K. pneumoniae LPS. The increase of proliferative activity was accompanied by a rise in the cytotovicity of mouse lymphocytes with respect to the NK-sensitive tumor line YAC-1 or Ehrlich tumor cells. The incubation of the lymphocytes with ripe DC (with the use of the preparations under study as ripening inducers), loaded with tumor antigens, made it possible to obtain cytotoxic lymphocytes having high cytotoxic activity with respect, mainly, to those tumor lines from which lysates for the treatment of DC had been produced. The activation of DC with bacterial immunomodulators led to an increase in the antigen-presenting function of these cells, to their higher capacity for regulating the differentiation of mononuclear leukocytes and for activating the cytotoxicity of natural killers.

Syngenic

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[The results of experimental and clinical studies of a multicomponent vaccine made of the antigens from opportunistic microorganisms].

PubMed (1998)

Egorova Nb Efremova Vn Kurbatova Ea Kuz'mina La

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[Protective activity of Immunovac-VP-4 vaccine against avian influenza virus H5N2 administered by different methods].

Zhurnal mikrobiologii epidemiologii i immunobiologii (2011)

Egorova Nb Kurbatova Ea Н. К. Ахматова Semenova Ib

AIM To experimentally assess protective effect of Immunovac-VP-4 vaccine against avian influenza virus H5N2. MATERIALS AND METHODS. Immunization of mice with polycomponent vaccine Immunovac-VP-4 was performed using oral or mucosal route of administration (intranasally, orally, and with combined nasal-oral method). Immunized mice were inoculated intranasally by influenza virus H5N2 adapted for mice. Survival of mice in experimental and control (intact) groups was assessed daily during 14 days. Survival and death rates of mice were determined. Levels of cytokines in sera of mice from both groups were measured by enzyme immunoassay. RESULTS Half of experimental animals survived after triple subcutaneous administration of vaccine in dose 20 mcg and subsequent intranasal challenge with avian influenza virus H5N2. Single subcutaneous immunization with dose 400 mcg resulted in survival of 80 +/- 12.6% of mice after challenge. Triple intranasal and combined intranasal-oral immunization as well as after triple subcutaneous immunization resulted in survival of half of challenged mice. In control group challenge was lethal for 90 - 100% of mice. Same methods of immunization lead to increase of IL-6, IL-12, IL-15, and IFN-gamma levels. CONCLUSION Data about significant protective effect after immunization with Immunovac-VP-4 against avian influenza virus H5N2 were obtained. Immunovac-VP-4 administered by mentioned routes activated nasal-associated lymphoid tissue providing first line defense at entry site of influenza infection, which demonstrates need to further study of this vaccine during development of strategy for non-specific prophylaxis of influenza infection.

Subcutaneous injection

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[Expression of Toll-like receptors in spleen and lymphatic nodes after immunization by mucosal routes].

Zhurnal mikrobiologii epidemiologii i immunobiologii (2010)

Н. К. Ахматова Egorova Nb Akhmatov Éa Kurbatova Ea Semenova Ib

AIM: To determine level of Toll-like receptors (TLRs) expression in spleen and lymphatic nodes of mice after immunization by mucosal routes. MATERIALS AND METHODS: Mice were immunized with polycomponent vaccine Immunovac either by mucosal or subcutaneous route. Expression of TLRs in spleen, respiratory tract-associated lymphatic nodes as well as in small intestine was measured in immunized mice by flow cytomentry method. RESULTS: After immunization of mice by subcutaneous, intranasal and oral routes level of TLRs expression was different. Significant expression of TLR9 and absence of TLR2 expression was noted after non-parenteral methods of immunization. After oral immunization expression of TLRs was identified in gut-and respiratory tract-associated lymphoid tissue as well as in spleen; after intranasal immunization--in respiratory tract-associated lymphoid tissue, and after subcutaneous immunization--in spleen and respiratory tract-associated lymphoid tissue. CONCLUSION: After oral immunization expression of TLRs was identified in all studied organs, including spleen. Involvement of spleen to this process allows to assume establishment of not only local but also systemic immunity.

Respiratory tract

TLR9

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[Use of therapeutic polycomponent vaccine "Immunovac VP-4" for the treatment of chronic bacterial prostatitis].

Zhurnal mikrobiologii epidemiologii i immunobiologii (2004)

Bondarenko Vm Vershinin Ae Kucherskiĭ Vm Mitkov Vg Klimova Ne

A complex of clinical and laboratory examinations of patients with bacterial chronic prostatitis was carried out. The patients were divided into two groups: 55 patients treated by the method of immunotherapy and 20 patients (controls) undergoing basic therapy. Immunotherapy was carried out with the use of polycomponent vaccine VP-4 containing Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli antigens. Vaccinal therapy was indicated in cases with infection focus associated with opportunistic bacteria, a prolonged torpid course of prostatitis and when different schemes of etiotropic therapy proved to be ineffective. The vaccine was introduced in 4 or 5 subcutaneous injections at an interval of 5-7 days. The study revealed that in cases of chronic prostatitis accompanied by microecological and immune disturbances the use of complex therapy allowed to achieve good clinical and laboratory results in 91% of the patients. In the patients undergoing immunotherapy the elimination of opportunistic bacteria from the urogenital tract and the restoration of affected microflora occurred.

Chronic bacterial prostatitis

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[Nonharsh methods of isolation of antigenic complexes of Klebsiella pneumoniae, and analysis of their protective activity].

PubMed (1983)

Egorova Nb Kurbatova Ea Мирошниченко Mashilova Gm Gladus Ma

Protective antigenic complexes capable of protecting mice from septic Klebsiella infection can be obtained from Klebsiella pneumoniae. The preparations obtained by the method of aqueous extraction and by the action of hydroxyl amine and autolysis have been found to possess approximately the same protective activity and to protect mice from infection with Klebsiella pneumoniae strains belonging to both homologous and heterologous serotypes.

Heterologous

Klebsiella

Autolysis (biology)

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