Objective To investigate the effects of sevoflurane on the expression of protein kinase C-α(PKC-α)in ischemia-reperfusion rat lungs. Methods 96 adult male wistar rats weighing 250~350 g were randomly divided into 4 groups:group C (n=24):continuous perfusion without ischemia;group IR(n =24):occlusion of the left pulmonary hilum for 45 min,followed by reperfusion;group sev-C(n =24):1MAC sevoflurane inhalation for 30 min,followed by continuous perfusion;group sev-IR (n=24):1MAC sevoflurane inhalation followed by ischemia- reperfusion. The lung wet-to-dry weight ratio (W/D)and lung permeability index (LPI)were calculated. The expressions of PKC-α in cytoplasm and cell membrane were determined by Western blot. Results W/D and LPI increased progressively after reperfusion in IR and sev-IR groups(P 0.05). Moreover,preadministration of sevoflurane could inhibit the increase of W/D and LPI(P 0.05). The expression of PKC-α in group IR in cellular membrane increased obviously after reperfusion (P 0.05),while in cytoplasm it decreased accordingly (P 0.05). The inhalation of sevoflurane could inhibit the change of PKC-α(P 0.05). Conclusion The activation and shifting of PKC-α may be associated with the lung injury after reperfusion. Sevoflurane inhibits the activation and shifting of PKC-α and protect the pulmonary function.
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