Summary Isolated protoplasts of 60 species of the Magnoliatae and 19 species of the Liliatae were embedded in droplets of agarose‐containing media in such a way that streaks of locally high cell densities were obtained. For comparison, agarose cultures with dispersed protoplasts and liquid cultures were prepared. Fast and reliable regeneration was obtained in several taxa. After transfer to low osmolar media, shoots were formed in 18 species belonging to the genera Arabidopsis, Asparagus, Biscutella, Brassica, Cichorium, Clianthus, Hypericum, Nicotiana, Petunia, Reseda, Senecio , and Solanum . Only protoplast‐derived callus which did not organize shoots was grown in species of Agrimonia, Daucus, Cucumis, Duchesnea, Helianthus, Potentilla, Prunus, Reseda, Solanum, Sorbus, Spinacia , and Vicia .

Protoplast

Callus

10.1111/j.1438-8677.1988.tb00038.x

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Citations (21)

Effect of different intervals of x-ray split doses on shoot production of in vitro derived explants of Gerbera jamesonii Bolus

F. Walther A. Sauer

Gerbera jamesonii

Bolus (digestion)

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Citations (2)

Influence of Acute and Fractionated X‐Ray Doses on Shoot Production of in Vitro Derived Explants of Gerbera jamesonii H. Bolus

Plant Breeding (1990)

F. Walther A. Sauer

Abstract In vitro mutation induction experiments will become economically relevant only if the genetic variation can be highly increased by this procedure. For that purpose the utilization of as high X‐ray doses as possible are considered an important prerequisite. It was the objective to investigate the effect of split versus acute X‐ray doses on in vitro derived micro shoots of Gebera jamesonii used as references system for plant species that can be propagated in vitro by axillary shoots. Splitting of doses between 10 Gy and 50 Gy into 2 or 3 fractions separated by periods of 4th each for recovery led to considerably rising shoot production. The recovery from damage, represented by accumulating the number of shoots developed during 16 weeks at 4 subsequent dates pf cutoff post‐treatment, by propagation profiles, and by calculation of the factor of recovery, resulted in the application of very high X‐ray doses only usable by means of multiple dose fraction procedures.

Gerbera jamesonii

Bolus (digestion)

10.1111/j.1439-0523.1990.tb00466.x

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Citations (4)