J. Kalmakoff

University of Otago

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Vernon K. Ward

University of Otago

C. C. Payne

University of Mississippi Medical Center

Alan J. Parkinson

The King's College

J. S. Pillai

Birla Institute of Technology and Science, Pilani - Dubai Campus

J.F. Longworth

Louisiana State University Agricultural Center

Chris N. Chilcott

University of Otago

Sean Davison

University of the Western Cape

Syd Moore

University of Otago

A. M. Crawford

Stanford University

Karl Gordon

Commonwealth Scientific and Industrial Research Organisation

Cooperative Institutions

University of Otago

Hebrew University of Jerusalem

Agricultural Research Organization

Centre National de la Recherche Scientifique

Graduate School Experimental Plant Sciences

University of California, Davis

Commonwealth Scientific and Industrial Research Organisation

Institute of Virology of the Slovak Academy of Sciences

Harvard University

Cornell University

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Comparative Study of 125 I- and [ 3 H]Acetate-Labeled Antibodies in Detecting Iridescent Viruses

Applied Microbiology (1974)

S. L. Bilimoria Alan J. Parkinson J. Kalmakoff

Radioimmunoassays for detecting cell-associated or released virus are described using either 125 I- or [ 3 H]acetate-labeled antibodies. In the first assay system, antigen-antibody complexes were separated from free antibody by centrifugation. Sensitivities of 0.1 μg of iridescent virus could be achieved with either 125 I- or [ 3 H]acetate-labeled antibody. In the second assay, the antigen was fixed to cover-slip cell cultures, and then reacted with labeled antibody, unbound radioactivity being removed by repeated washing. Nonspecific binding with this method was 0.5 to 1% of the total radioactivity added and sensitivities of 0.1 or 10 μg were achieved with 125 I and [ 3 H]acetate, respectively. Immunoglobulins were labeled at the rate of 1 in 300 for 125 I and 1 in 200 with [ 3 H]acetate although there was a 400-fold greater isotopic abundance of 125 I relative to 3 H. The possibility of preparing labeled protein of high specific activity using carrier-free [2- 3 H]iodoacetic acid is discussed.

Iodoacetic acid

10.1128/am.28.1.133-137.1974

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Citations (8)

Changes in Anti-DNA Antibody Affinity during Exacerbations of Systemic Lupus Erythematosus

Scandinavian Journal of Rheumatology (1986)

Greg Hale John Highton J. Kalmakoff D. G. Palmer

AbstractThe level and average functional affinity of anti-DNA antibody were measured retrospectively in successive serum samples from 5 patients with systemic lupus erythematosus. In 2 patients, severe flares of disease activity were associated with an increase in the level and average functional affinity of anti-DNA antibodies. Three patients who did not experience significant flares in disease activity exhibited more constant levels of antibody of lower average functional affinity. The appearance of antibody of high functional affinity during disease exacerbations suggests that such antibodies may be pathogenic.

10.3109/03009748609092586

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Citations (10)

Detection of virus-specific immunoglobulins using a doubly labeled fluorescein-125I antibody

Journal of Clinical Microbiology (1976)

Alan J. Parkinson J. Kalmakoff

Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not vary by more than one twofold dilution when compared by the two methods.

Antibody titer

10.1128/jcm.3.6.637-639.1976

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A comparison of the structural polypeptides of three iridescent viruses (types 6, 9, and 16) and the mapping of the DNA region coding for their major capsid polypeptides

Archives of Virology (1992)

Sean Davison Alan Carne Nigel A.J. McMillan J. Kalmakoff

10.1007/bf01317153

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Citations (13)

Physical mapping of the DNA genome of insect iridescent virus type 9 from Wiseana spp. larvae

Virology (1987)

Vernon K. Ward J. Kalmakoff

Restriction map

Iridescence

Genome size

Physical mapping

10.1016/0042-6822(87)90027-4

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Citations (38)

Insect Iridescent Virus Type 9 and Type 16

Springer eBooks (1990)

J. Kalmakoff Nigel A.J. McMillan Sean Davison

Homology

Iridescence

10.1007/978-1-4613-1615-2_5

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Citations (8)

Effect of Alkaline Protease on the Antigenic Nature of Wiseana Nuclear Polyhedrosis Virus Polyhedron Protein

Journal of Virology (1977)

A. M. Crawford J. Kalmakoff

Polyhedron protein from Wiseana spp. nuclear polyhedrosis virus was found to be degraded by an alkali protease when polyhedra are dissolved in alkali. The protease activity did not occur at high pH (0.1 M NaOH) and was inactivated by heating polyhedra to 70 degrees C for 3 h. The products from the protease degradation of Wiseana spp. nuclear polyhedrosis virus polyhedron protein retain the antigenicity of undegraded polyhedron protein as measured by the direct solid-phase radioimmunoassay and immunoadsorption. Degradation products below 27,000 daltons could not be detected by the sandwich radioimmunoassay, indicating that they are probably monovalent.

Antigenicity

Nuclear Polyhedrosis Virus

Polyhedron

Immunoadsorption

10.1128/jvi.24.1.412-415.1977

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Citations (21)

Physicochemical Properties of Tipula Iridescent Virus

Journal of Virology (1968)

J. Kalmakoff J. H. Tremaine

The molecular weight of Tipula iridescent virus, based on sedimentation and diffusion coefficients, was 5.51 x 10(8), with hydration of 0.57 g of water per g of virus. Deoxyribonucleic acid content, based on total inorganic phosphorus liberated, was 19 +/- 0.2%. At 260 mmu, the virus gave an uncorrected absorbance of 18.2 cm(2)/mg of virus and a light-scattering corrected absorbance of 9.8 cm(2)/mg of virus. Amino acid analyses of the virus protein revealed a remarkable similarity to Sericesthis iridescent virus. The possibility is discussed that the four iridescent insect viruses reported to date bear a strain relationship.

Iridescence

Absorbance

Strain (injury)

10.1128/jvi.2.7.738-744.1968

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Citations (28)

A radioimmunoassay using labeled antibody for polyhedron protein from a nuclear polyhedrosis virus of Wiseana cervinata

Journal of Invertebrate Pathology (1977)

J. Kalmakoff A. M. Crawford Syd Moore

Nuclear Polyhedrosis Virus

Polyhedron

10.1016/0022-2011(77)90168-9

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Citations (9)

An iridescent virus and a rickettsia from the grass grubCostelytra zealandica(Coleoptera: Scarabaeidae)

New Zealand Journal of Zoology (1974)

Syd Moore J. Kalmakoff J. A. R. Miles

Natural iridescent virus and rickettsia infections of Costelytra zealandica (White) and Odontria sp. indet. larvae were studied at a site in the upper Pareora Gorge scenic reserve, S. Canterbury. By sequentially sampling the site, it was found that neither the iridescent virus nor the rickettsiae appear to give rise to host mortalities that significantly alter the population density. Many larvae were found that appeared healthy, but carried inapparent iridescent virus infections. These diseases are not suitable for biological control of the grass grub.

10.1080/03014223.1974.9517828

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Citations (10)