Masayo Masu

Kindai University

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Mamoru Koketsu

Gifu University

Ayaka Tsuge

National Cancer Centre Japan

Yuji Muramatsu

Gifu University

Masayuki Ninomiya

Gifu University

Masaki Taniguchi

Osaka University Hospital

Masashi Andô

Kindai University

Tomohiro Itoh

Mie University

Yasuyuki Tsukamasa

Kindai University

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Kindai University

Gifu University

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Andalas University

Nagoya University

Kyoto University

Kyoto Prefectural University

Mansoura University

Hokuriku University

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A Synthesized Nostocionone Derivative Potentiates Programmed Cell Death in Human T-cell Leukemia Jurkat Cells Through Mitochondria via the Release of Endonuclease G

Nutrition and Cancer (2014)

Tomohiro Itoh Yuji Muramatsu Masayo Masu Ayaka Tsuge Masaki Taniguchi

AbstractNostocionone (Nost), a compound isolated from Nostoc commune, and its synthesized derivatives (NostDs) were evaluated for in vitro cytotoxicity against human T-cell leukemia Jurkat cells. NostD3 [(1E,4E)-1-(3,4-dihydroxyphenyl)-5-(2,6,6-trimethylcyclohex-1-enyl)penta-1,4-dien-3-one] inhibited cell growth more potently than Nost. To elucidate the mechanisms of NostD3-induced cell death, we examined changes in cell morphology, the loss of mitochondrial membrane potential (MMT), and DNA fragmentation. From these results, the cytotoxic effects of NostD3 were found to be mainly due to Type I programmed cell death (PCDI; i.e., apoptosis). Using caspase inhibitors, we further found that NostD-3-induced PCDI occurred through a caspase-independent pathway. Moreover, NostD3 decreased MMT and modulated multiple signaling molecules (MAPKs, Akt, Bcl-2, Bax, and c-Myc) in Jurkat cells, thereby inducing the release of endonuclease G (Endo-G) from mitochondria. The level of intracellular reactive oxygen species (ROS) in cells treated with NostD3 was elevated up to 1 h after the treatment. However, suppression of ROS by N-acetyl-l-cysteine restored Jurkat cell growth. Taken together, our data suggested that ROS production acted as a trigger in NostD3-induced PCDI in Jurkat cells through release of Endo-G from the mitochondria.

Jurkat cells

10.1080/01635581.2014.956255

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