R. K. Vijh

National Bureau of Animal Genetic Resources

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

M. S. Tantia

National Bureau of Animal Genetic Resources

Bina Mishra

National Bureau of Animal Genetic Resources

Rahul Behl

National Bureau of Animal Genetic Resources

Jyotsna Behl

National Bureau of Animal Genetic Resources

Jyoti Joshi

Center for Molecular Dynamics - Nepal

Priyanka Banerjee

Auburn University

Reena Arora

National Bureau of Animal Genetic Resources

Sonika Ahlawat

National Bureau of Animal Genetic Resources

S. K. Niranjan

National Bureau of Animal Genetic Resources

Upasna Sharma

National Bureau of Animal Genetic Resources

Cooperative Institutions

National Bureau of Animal Genetic Resources

Indian Council of Agricultural Research

National Dairy Research Institute

Indian Veterinary Research Institute

University of Delhi

University of Toronto

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

Indian Agricultural Research Institute

Chaudhary Charan Singh Haryana Agricultural University

National Research Centre on Equines

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Evaluation of 24 microsatellite markers for parentage exclusion in three indigenous pig types of India

The Indian Journal of Animal Sciences (2017)

Rahul Behl Jyotsna Behl M. S. Tantia N. Nahardeka Gobinda Das

A set of 24 FAO recommended microsatellite markers was evaluated for parentage exclusion (PE) in three Indian pig types of Assamese, North-Indian and Ankamali. The genomic DNA from these three Indian pig types was amplified at these loci by polymerase chain reaction and resolved for alleles. The allelic frequency data was analysed to calculate the probability of paternity exclusion when one of the parents is to be excluded (PE1), when both the parents are to be excluded (PE2) and when only one parent is known and that is to be excluded (PE3) in these three Indian pig types using a set of minimum 5 loci and then increasing the number of loci in increments of 5 upto maximum of 24 loci. The cumulative PE1 values taking into consideration all the 24 loci varied from 1-2.07×10-10 in North Indian pigs to 1-3.95×10-11 in Ankamali pigs. The cumulative PE2 values taking into consideration all the 24 loci varied from 1-4.57×10-16 in Assamese pigs to 1-3.17×10-18 in Ankamali pigs. Similarly, cumulative PE3 values for all the 24 loci varied from 0.9999968 in Assamese pigs to 0.99999955 in Ankamali pigs. The cumulative PE values obtained, even with a set of 15 loci (CGA, IGF1, S0005, S0026, S0068, S0090, S0155, S0178, S0215, S0218, S0228, S0355, SW122, SW911, SW936), were clearly more than the required value of 0.9995 in all the three breeds with the minimum value of 0.99985 for PE3 in Assamese pigs. Clearly, this set of 15 loci or the sets with 20 or 24 loci can be safely employed for parentage exclusion purposes in the Indian pigs.

Assamese

10.56093/ijans.v87i4.69523

Cite

Citations (2)

Genetic diversity analysis of two buffalo populations of northern India using microsatellite markers

Journal of Animal Breeding and Genetics (2004)

Reena Arora B. D. Lakhchaura Rajendra Prasad M. S. Tantia R. K. Vijh

Summary The genetic diversity in two buffalo populations of northern India, the Bhadawari and the Tarai was assessed using a set of 22 heterologous (bovine) microsatellite markers. The average number of alleles across all loci in both populations was found to be 4.7, indicating that this set of 22 bovine microsatellite markers could be used to study genetic variation in buffalo species also. The overall polymorphic information content (PIC) value for these markers was 0.54. The average observed and expected heterozygosities for both populations were 0.59 and 0.64, respectively. Common alleles with varying allele frequencies in both populations also represented the genetic variability existing between Bhadawari and Tarai buffaloes. However the θ estimates for population differentiation indicated low levels of differentiation between the two populations. This was further supported by the low genetic distance (0.155) between Bhadawari and Tarai, which was calculated using Nei's standard genetic distance method. The present study on Bhadawari and Tarai populations represents a much‐needed preliminary effort that could be extended to other local buffalo populations of India as well.

Genetic distance

10.1111/j.1439-0388.2004.00451.x

Cite

Citations (43)

Characterization of Punjab Brown chicken

Animal Genetic Resources Information (2006)

P. K. Vij M. S. Tantia R. K. Vijh

Summary A survey was conducted in the native tract of the Punjab Brown breed of chicken to study management practices, as well as morphological, performance and egg quality parameters. The study covered the three districts of Gurdaspur district in Punjab, and Ambala and Yamunanagar districts in Haryana, and included 532 birds and 61 families. Twenty-six microsatellite loci were used to assess genetic variability. The Punjab Brown is a multi-purpose breed, yielding good quality meat and eggs. Birds are reared in the backyard system and shelter is provided only during the night in the form of small enclosures mostly made up of mud and sometimes of wood. Average flock size is 8.7. Plumage colour is mostly brown and the pattern is usually solid but is sometimes spotted or striped. Males in particular have black spots/stripes on their neck, wings and tail. The comb is red, of single type and erect in position. The average weight of cocks and hens is 2.15±0.94 and 1.57±0.04 kg respectively. Hens start laying eggs at the age of about five to six months. Clutch size is about four to five. Average egg production is around 60–80 eggs per year. Eggshell colour is mostly light brown and average egg weight is 46.0±1.91g. The average weight of shell, albumin and yolk were 5.4±0.21, 24.4±0.63 and 16.2±0.48g respectively. Yolk index, albumin index and Haugh units were 0.41±0.005, 0.10±0.006 and 82.80±0.98 respectively. A total of 218 alleles were observed. The number of alleles per locus varied from 4–14. The mean PIC value for all the loci was 0.744. Twenty-four loci were found to be neutral (P<0.05) using Ewens Watterson test of neutrality. The exact test revealed that 15 loci deviated from Hardy Weinberg Equilibrium. The population has not undergone any recent bottleneck as revealed by quantitative and graphical qualitative tests.

Flock

Yolk

Haugh unit

Plumage

10.1017/s1014233900002145

Cite

Citations (18)

Molecular phylogenetic analysis of the sequences of candidate genes involved in milk production traits in riverine buffalo (Bubalus bubalis)

International Journal of Livestock Production (2011)

Aditi Sharma Shamsher S. Kanwar M. S. Tantia R. K. Vijh Baldi Bypass

Domestic buffalo and cattle are two extremely important livestock species in worldwide agricultural production. Despite some similarities with respect to morphologic and genetic characters, cattle and buffalo are divergent evolutionarily and are classified as different genera within the subfamily of Bovinae (Bos and Bubalus). The present study aimed at partial bayesian phylogenetic reconstruction of bovini tribe (Bovidae, Bovinae) from cDNA of 7 autosomal genes. Divergence times between cattle and buffalo were estimated using a relaxed molecular clock using calibration points based on best estimates of divergence times in the fossil record for Suina-Ruminantia and Laurasiatheria-Euarchontoglires splits. In the present analysis two calibration points were accessed. The two bovine subtribes consistently resolved themselves as a dichotomous group with strong support for a bifurcation between representatives of bovina and bubalina subtribes. Based on the molecular calibrations divergence time of buffalo and cattle was estimated to be 10.4 MYA. The mouse and rat split was estimated to be 36.6 MYA. The results are in agreement with the previous studies being carried out different research groups. Key words: Phylogenetics, molecular calibration, bovidae, bovinae, divergence time, buffalo, cattle, laurasiatheria, ruminantia, euarchontoglires.

Bubalus

Bovidae

Subfamily

10.5897/ijlp.9000019

Cite

Citations (0)

Genetic and demographic bottleneck analysis of malvi camel breed by microsatellite markers

Journal of Camel Practice and Research (2015)

Sushma Prasad Sharique A. Ali R. K. Vijh

The study included genetic and demographic bottleneck analysis of Malvi camel breeds by microsatellite markers using 29 loci. The genetic diversity of a population of Malvi camel was evaluated. Multilocus genotype data on 29 microsatellites were generated on 138 Malvi camels. A total of 240 alleles were observed. The mean observed heterozygosity in camel population for all the 29 loci was found to be 0.6041 ± 0.3256 while the mean expected heterozygosity was 0.5978 ± 0.1983. The statistical values for within locus k-test obtained for the Malvi camel was 18 loci with negative values which signifies population expansion. The inter locus g-test revealed a value of 2.3557. This test also revealed that Malvi camel population has undergone population expansion. Three tests viz; Sign test (Non-parametric), Standardised Differences test (Parametric), Wilcoxon test and Qualitative graphical method based on the allele frequency spectra reflects that the population had not undergone bottleneck.

Genetic Analysis

10.5958/2277-8934.2015.00002.8

Cite

Citations (0)

Cytogenetics of equines with special reference to breeding & reproductive disorders: a review.

R. K. Vijh R. Sahai Anupma Sharma

Source

Cite

Citations (0)

Evaluation of buffalo populations for mutation-drift equilibrium

The Indian Journal of Animal Sciences (2005)

R. K. Vijh Bina Mishra Preeti Chaudhary R. K. Singh Reena Arora

The data was generated for 24 microsatellite in 3 buffalo populations/breeds viz; Bhadawari, Tarui and local buffaloes of Kerala. Both qualitative, as well as quantitative tests were conducted for testing the population for mutation-drift equilibrium.The data was analysed for heterozygosity excess and allelic distribution. Standardized difference test and Wilcoxon rank test being parametric were utilized in all the 3 mutation models to draw inferences. The TPM revealed Kerala buffaloes and Tarai buffaloes to have experienced populotion bottlenecks in the last 100-250.generation.The qualitative (graphical) test, however, did not exhibit and 'mode shift' distortion in all the 3 population of buffaloes and gave normal L-shaped curve.

Source

Cite

Citations (0)

Characterization of Osteopontin gene of Bubalus bubalis

animal (2008)

M. S. Tantia Bishnu Prasad Mishra Sanjay Kumar Bishnu Prasad Mishra R. S. Kataria

Osteopontin, a glycoprotein, is expressed in several tissues including the mammary gland. The gene has been reported to be associated with milk and its constituents in various livestock species. This gene was sequenced in buffalo and it coded for the protein of 280 amino acids with the conserved GRGDS domain. The sequence was confirmed from the cDNA sequence derived from the mammary gland of buffalo. The earlier-reported 9T/10T variation in the upstream region of the gene was investigated for its effect on milk in buffalo and it was found to be non-significant.

Bubalus

Osteopontin

10.1017/s1751731108002073

Cite

Citations (10)

Performance characteristics of Nagori cattle

The Indian Journal of Animal Sciences (1995)

K. Vij M. S. Tantia A. E. Nivsarkar R. K. Vijh P. Suresh Kumar

Information collected from 145 Nagori animals maintained at the Govemment Cattle Breeding Farm, Nagaur (from 1975 to 1992) was analysed by least-squares maximum likelihood technique. The least-squares means for production traits, viz. milk yield, lactation length, milk yield/day of lactation, milk yield/day of calving interval and dry period were 535.3± 18.13 kg, 267.7±7.77 days, 2.1±0,07 kg, 1.3±0.04 kg and 154.7±10,35 days, respectively, and for reproduction trailS, viz. service period, gestation period and calving interval, were 120.6±5.94, 283.6±0.85 and 423.2±11.22 days respectively. Average age at first calving was 1503.2±26.92 days and birth weight of calf was 16.9±0.16 kg. Parity had significant influence on all the traits except lactation length, gestation period and birth weight. Month of calving affected significantly aU the production traits except dry period and only service period among the reproduction traits. Period of calving had significant influence on milk yield traits, gestation period and calving interval. Male calves had significantly higher birth weight than the female calves. Repeatability and phenotypic correlations among different traits were also estimated.

Gestation period

Repeatability

Source

Cite

Citations (0)

Comparative evaluation of three buffalo populations using microsatellite markers

The Indian Journal of Animal Sciences (2005)

R. K. Vijh Bina Mishra Reena Arora Preeti chaudhry Uma Sharma

Data generated on 24 microsatellite loci from 3 buffalo populations viz. Bhadawari, Tarai and Kerala buffaloes was subjected to analysis for estimation of genetic distances. The genetic distances calculated were Nei's minimum, Nei's standard, Latter's Fst, Reynolds' distances (IAM). The genetic distances based on SMM were also estimated. These were average squared distance (ASD), delta mu square [()'], Shrivers' Dsw and similarity index (D,). The dendrograms were prepared using both UPGMA and NJ algorithms. The Tarai and Bhadawari populations were close to one another and this was expected because of the contiguity of their breeding tract resulting in increased gene flow. The results of all the genetic distances were similar except ASD which is not the right method for closely related populations of a species. The inter-individual distances were also estimated and clustering of individuals was done utilizing UPGMA and NJ methods.

UPGMA

Dendrogram

Genetic distance

Similarity (geometry)

Genetic similarity

Source

Cite

Citations (3)