Lorena Oliveira Souza Soares

Escola Nacional de Saúde Pública

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Enrico Mendes Saggioro

Fundação Oswaldo Cruz

Fábio Veríssimo Correia

Fundação Oswaldo Cruz

Sidney Fernandes Sales

Escola Nacional de Saúde Pública

Gabriel de Farias Araújo

Fundação Oswaldo Cruz

Rachel Ann Hauser‐Davis

Fundação Oswaldo Cruz

Tatiana Dillenburg Saint’Pierre

Pontifical Catholic University of Rio de Janeiro

Rafael Christian Chávez Rocha

Pontifical Catholic University of Rio de Janeiro

Cláudio Ernesto Taveira Parente

Instituto de Biofísica Carlos Chagas Filho

FÃ

Fabrício Ãngelo Gabriel

Universidade Federal do Espírito Santo

Evelyn Oliveira da Silva

Fundação Oswaldo Cruz

Cooperative Institutions

Fundação Oswaldo Cruz

Universidade Federal do Rio de Janeiro

Escola Nacional de Saúde Pública

Universidade Federal do Estado do Rio de Janeiro

Universidade de São Paulo

Universidade do Estado do Rio de Janeiro

Universidade Federal Fluminense

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Pontifical Catholic University of Rio de Janeiro

Instituto de Biofísica Carlos Chagas Filho

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Rare Earth Element Accumulation in Fiddler Crabs (Minuca rapax) from the Rio Doce Tropical Estuary Strongly Affected by Mine Tailings Following the Fundão Disaster

Biological Trace Element Research (2024)

Sidney Fernandes Sales Fabrício Ãngelo Gabriel Lorena Oliveira Souza Soares Rafael Christian Chávez Rocha Tatiana Dillenburg Saint’Pierre

Fiddler crab

Rare-earth element

10.1007/s12011-024-04231-3

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Citations (0)

?3?1-integrin as a critical mediator of the hepatic differentiation response to the extracellular matrix

Hepatology (1998)

José M. Lora Kathleen E. Rowader Lorena Oliveira Souza Soares Filippo G. Giancotti Kenneth S. Zaret

The extracellular matrix (ECM) promotes the differentiation of many cell types, and ECM remodeling in the liver has been implicated in embryonic development, tissue injury, and oncogenesis. Integrins are heterodimeric ECM receptors that play critical roles in transducing the composition of the ECM in the cell environment. We previously showed that mouse H2.35 cells, a conditionally transformed, liver-derived cell line, assume a more differentiated hepatocyte morphology and enhanced liver-specific gene expression when the cells are cultured on gelatinous ECM substrata. Here we show that H2. 35 cells express relatively high levels of alpha3beta1-integrins, similar to that previously shown for immature hepatocytes, transformed hepatocytes, and biliary cells. However, the cell morphological responses that depend on alpha3beta1-integrin have not been defined. We found that transfecting H2.35 cells with antisense RNA construct directed to alpha3-subunit messenger RNA perturbs the initial cell attachment to laminin and collagen, and strongly inhibits cell morphological, proliferative, and gene expression responses to a collagen gel substratum. In situ hybridization to mouse embryo tissues demonstrates the presence of alpha3-subunit messenger RNAs in newly formed hepatocytes. We suggest that alpha3beta1-integrins are important for immature and transformed hepatocytes to respond morphologically to the extracellular matrix.

10.1002/hep.510280426

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Citations (46)

Sub-lethal effects of the pesticide imazalil on the earthworm Eisenia andrei: reproduction, cytotoxicity, and oxidative stress

Environmental Science and Pollution Research (2019)

Patrícia Christina Genázio Pereira Lorena Oliveira Souza Soares Sidney Fernandes Sales Enrico Mendes Saggioro Fábio Veríssimo Correia

Eisenia andrei

Chronic toxicity

10.1007/s11356-019-05440-3

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Citations (20)

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

PeerJ (2020)

Fabrício Ãngelo Gabriel Rachel Ann Hauser‐Davis Lorena Oliveira Souza Soares Ana Carolina A. Mazzuco Rafael Christian Chávez Rocha

Background The Rio Doce estuary, in Brazil, was impacted by the deposition of iron mine tailings, caused by the collapse of a dam in 2015. Based on published baseline datasets, the estuary has been experiencing chronic trace metal contamination effects since 2017, with potential bioaccumulation in fishes and human health risks. As metal and metalloid concentrations in aquatic ecosystems pose severe threats to the aquatic biota, we hypothesized that the trace metals in estuarine sediments nearly two years after the disaster would lead to bioaccumulation in demersal fishes and result in the biosynthesis of metal-responsive proteins. Methods We measured As, Cd, Cr, Cu, Fe, Mn, Pb, Se and Zn concentrations in sediment samples in August 2017 and compared to published baseline levels. Also, trace metals (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Se and Zn) and protein (metallothionein and reduced glutathione) concentrations were quantified in the liver and muscle tissues of five fish species ( Cathorops spixii , Genidens genidens , Eugerres brasilianus , Diapterus rhombeus and Mugil sp.) from the estuary, commonly used as food sources by local populations. Results Our results revealed high trace metal concentrations in estuarine sediments, when compared to published baseline values for the same estuary. The demersal fish species C. spixii and G. genidens had the highest concentrations of As, Cr, Mn, Hg, and Se in both, hepatic and muscle, tissues. Trace metal bioaccumulation in fish was correlated with the biosynthesis of metallothionein and reduced glutathione in both, liver and muscle, tissues, suggesting active physiological responses to contamination sources. The trace metal concentrations determined in fish tissues were also present in the estuarine sediments at the time of this study. Some elements had concentrations above the maximum permissible limits for human consumption in fish muscles (e.g., As, Cr, Mn, Se and Zn), suggesting potential human health risks that require further studies. Our study supports the high biogeochemical mobility of toxic elements between sediments and the bottom-dwelling biota in estuarine ecosystems.

Demersal fish

Trace metal

Metallothionein

Biota

10.7717/peerj.10266

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Citations (69)

Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma

Journal of Cellular Physiology (2019)

Jéssica Honorato Ribeiro Rachel Ann Hauser‐Davis Enrico Mendes Saggioro Fábio Veríssimo Correia Sidney Fernandes Sales

Abstract The first‐line chemotherapy treatment for Glioblastoma (GBM) ‐ the most aggressive and frequent brain tumor ‐ is temozolomide (TMZ). The Sonic hedgehog (SHH) pathway is involved with GBM tumorigenesis and TMZ chemoresistance. The role of SHH pathway inhibition in the potentiation of TMZ's effects using T98G, U251, and GBM11 cell lines is investigated herein. The combination of GANT‐61 and TMZ over 72 hr suggested a synergistic effect. All TMZ‐resistant cell lines displayed a significant decrease in cell viability, increased DNA fragmentation and loss of membrane integrity. For T98G cells, G 2 /M arrest was observed, while U251 cells presented a significant increase in reactive oxygen species production and catalase activity. All the cell lines presented acidic vesicles formation correlated to Beclin‐1 overexpression. The combined treatment also enhanced GLI1 expression, indicating the presence of select resistant cells. The selective inhibition of the SHH pathway potentiated the cytotoxic effect of TMZ, thus becoming a promising in vitro strategy for GBM treatment.

Temozolomide

Viability assay

10.1002/jcp.29274

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Citations (26)

Antioxidant System Alterations and Biological Health Status of Earthworms Following Long-term Exposure to Antibiotics Contaminated Poultry Litter

Research Square (Research Square) (2021)

Sidney Fernandes Sales Evelyn Oliveira da Silva Gabriel de Farias Araújo Lorena Oliveira Souza Soares Cláudio Ernesto Taveira Parente

Abstract Poultry litter is widely applied as a fertilizer even though it is one of the main antibiotic sources for agricultural soils. Long-term sublethal effects (56 days) on the antioxidant system of Eisenia andrei earthworms following exposure to fluoroquinolone-contaminated poultry litter (enrofloxacin + ciprofloxacin) at 5.0, 10 and 20 g kg -1 were evaluated. The following biomarkers were assessed: superoxide dismutase (SOD), catalase (CAT), glutathione-S-trasferase (GST), reduced glutathione (GSH) and a lipid peroxidation (LPO) proxy. Significant CAT and SOD increases and a moderate positive correlation (ρ = 0.67, p < 0.05) between these enzymes was observed. Glutathione-S-trasferase levels increased significantly at 10 g kg -1 , while GSH exhibited a dose-dependent response at 5.0 mg kg -1 (4 - 106%), 10 mg kg -1 (28 - 330 %) and 20 mg kg -1 (45 - 472%). LPO levels exhibited a decreasing trend with increasing poultry litter concentrations of 8 - 170% (5.0 g kg -1 ), 7 - 104% (10 mg kg -1 ) and 3 - 6% (20 mg kg -1 ). A Principal Component Analysis (PCA) highlighted increased SOD and CAT activities, possibly due to increases in reactive oxygen species (ROS) concentrations. Biological Health Status assessments based on the Biomarker Response Index indicate major alterations in the first month of exposure, changing to moderate in the second month. These findings indicate an antioxidant system attenuation trend. It is possible, however, that successive poultry litter applications may reduce the long-term recovery capacity of the evaluated biomarkers.

Poultry litter

Litter

10.21203/rs.3.rs-595228/v1

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Antioxidant system alterations, oxidative, and genotoxic effects in Danio rerio (zebrafish) exposed to leachate from a dumpsite

Environmental Science and Pollution Research (2024)

Sidney Fernandes Sales Nicolle Martins da Costa Gabriel de Farias Araújo Lorena Oliveira Souza Soares Camille Ferreira Mannarino

Comet Assay

Danio

Malondialdehyde

10.1007/s11356-024-31883-4