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Kandiah Jeyaseelan

National University of Singapore

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Arunmozhiarasi Armugam

Institute of Bioengineering and Nanotechnology

Sugunavathi Sepramaniam

Experimental Drug Development Centre

Dwi Setyowati Karolina

National University of Singapore

E. Marelyn Wintour

Monash University

Linda Earnest

Peter Doherty Institute

P. Gopalakrishnakone

National University of Singapore

Tai‐Shung Chung

National Taiwan University of Science and Technology

Prameet Kaur

Yale-NUS College

M.C.M. Chung

National University of Singapore

Yen Wah Tong

National University of Singapore

Cooperative Institutions

National University of Singapore

National University Health System

National University Hospital

University of Melbourne

Florey Institute of Neuroscience and Mental Health

National University Heart Centre Singapore

University Health System

Monash University

Agency for Science, Technology and Research

University of Malaya

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Ovine AQP 1 : cDNA cloning, ontogeny, and control of renal gene expression

Pediatric Nephrology (1998)

E. Marelyn Wintour Linda Earnest Daine Alcorn Aldona Butkus Lynne Shandley

Aquaporin 1

Glyceraldehyde 3-phosphate dehydrogenase

Renal cortex

10.1007/s004670050502

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Citations (29)

Proteasome inhibition by lactacystin in primary neuronal cells induces both potentially neuroprotective and pro‐apoptotic transcriptional responses: a microarray analysis

Journal of Neurochemistry (2005)

Elaine Hau Jin Yew Nam Sang Cheung M.S. Choy Robert Z. Qi Alan Yiu Wah Lee

Abstract Although inhibition of the ubiquitin proteasome system has been postulated to play a key role in the pathogenesis of neurodegenerative diseases, studies have also shown that proteasome inhibition can induce increased expression of neuroprotective heat‐shock proteins (HSPs). The global gene expression of primary neurons in response to treatment with the proteasome inhibitor lactacystin was studied to identify the widest range of possible pathways affected. Our results showed changes in mRNA abundance, both at different time points after lactacystin treatment and at different lactacystin concentrations. Genes that were differentially up‐regulated at the early time point but not when most cells were undergoing apoptosis might be involved in an attempt to reverse proteasome inhibitor‐mediated apoptosis and include HSP70, HSP22 and cell cycle inhibitors. The up‐regulation of HSP70 and HSP22 appeared specific towards proteasome inhibitor‐mediated cell death. Overexpression of HSP22 was found to protect against proteasome inhibitor‐mediated loss of viability by up to 25%. Genes involved in oxidative stress and the inflammatory response were also up‐regulated. These data suggest an initial neuroprotective pathway involving HSPs, antioxidants and cell cycle inhibitors, followed by a pro‐apoptotic response possibly mediated by inflammation, oxidative stress and aberrant activation of cell cycle proteins.

Lactacystin

Viability assay

10.1111/j.1471-4159.2005.03220.x

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MicroRNA-10b pleiotropically regulates invasion, angiogenicity and apoptosis of tumor cells resembling mesenchymal subtype of glioblastoma multiforme

Cell Death and Disease (2012)

Jiakai Lin Siew Hoon Teo Dang Hoang Lam Kandiah Jeyaseelan S Wang

Glioblastoma multiforme (GBM) is a heterogeneous disease despite its seemingly uniform pathology. Deconvolution of The Cancer Genome Atlas's GBM gene expression data has unveiled the existence of distinct gene expression signature underlying discrete GBM subtypes. Recent conflicting findings proposed that microRNA (miRNA)-10b exclusively regulates glioma growth or invasion but not both. We showed that silencing of miRNA-10b by baculoviral decoy vectors in a glioma cell line resembling the mesenchymal subtype of GBM reduces its growth, invasion and angiogenesis while promoting apoptosis in vitro. In an orthotopic human glioma mouse model, inhibition of miRNA-10b diminishes the invasiveness, angiogenicity and growth of the mesenchymal subtype-like glioma cells in the brain and significantly prolonged survival of glioma-bearing mice. We demonstrated that the pleiotropic nature of miRNA-10b was due to its suppression of multiple tumor suppressors, including TP53, FOXO3, CYLD, PAX6, PTCH1, HOXD10 and NOTCH1. In particular, siRNA-mediated knockdown experiments identified TP53, PAX6, NOTCH1 and HOXD10 as invasion regulatory genes in our mesenchymal subtype-like glioma cells. By interrogating the REMBRANDT, we noted that dysregulation of many direct targets of miRNA-10b was associated with significantly poorer patient survival. Thus, our study uncovers a novel role for miRNA-10b in regulating angiogenesis and suggests that miRNA-10b may be a pleiotropic regulator of gliomagenesis.

Oncomir

10.1038/cddis.2012.134

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Citations (130)

Molecular cloning, characterization and evolution of the gene encoding a new group of short‐chain α‐neurotoxins in an Australian elapid, Pseudonaja textilis

FEBS Letters (2000)

Nanling Gong Arunmozhiarasi Armugam Kandiah Jeyaseelan

The structure and organization of five genes responsible for the synthesis of six isoforms of short‐chain α‐neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes ( Pt‐sntx1 ) as a result of alternative splicing. Each gene consists of three exons which are separated by two introns and each has a functional promoter. The promoter activity was confirmed by both CAT assay and Real‐Time PCR. A transcription initiation site, two putative TATA boxes, one CCAAT box and the transcription factor binding consensus sites for AP‐1, GATA‐2, c/EBPb were identified in the 5′ non‐coding region of each gene. Phylogenetic analysis showed that these five genes from P. textilis constituted a distinct group which has evolved by gene duplication followed by accelerated evolution from an ancestral gene.

10.1016/s0014-5793(00)01549-0

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A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken α7 nicotinic acetylcholine receptors

European Journal of Biochemistry (2002)

Siew Lay Poh Gilles Mourier Robert Thaï Arunmozhiarasi Armugam Jordi Molgó

Weak neurotoxins from snake venom are small proteins with five disulfide bonds, which have been shown to be poor binders of nicotinic acetylcholine receptors. We report on the cloning and sequencing of four cDNAs encoding weak neurotoxins from Naja sputatrix venom glands. The protein encoded by one of them, Wntx‐5, has been synthesized by solid‐phase synthesis and characterized. The physicochemical properties of the synthetic toxin (sWntx‐5) agree with those anticipated for the natural toxin. We show that this toxin interacts with relatively low affinity ( K d = 180 n m ) with the muscular‐type acetylcholine receptor of the electric organ of T. marmorata , and with an even weaker affinity (90 µ m ) with the neuronal α7 receptor of chicken. Electrophysiological recordings using isolated mouse hemidiaphragm and frog cutaneous pectoris nerve–muscle preparations revealed no blocking activity of sWntx‐5 at µ m concentrations. Our data confirm previous observations that natural weak neurotoxins from cobras have poor affinity for nicotinic acetylcholine receptors.

Neurotoxin

10.1046/j.1432-1033.2002.03113.x

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Citations (43)

Characterizing Behavior of Corpus Cavernosum in Chloride-free Condition

Lang-Chu Lau P.G. Adaikan Arunmozhiarasi Armugam Kandiah Jeyaseelan

Tetraethylammonium chloride

Chloride channel

Niflumic acid

10.1016/j.urology.2011.01.018

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MicroRNAs as therapeutic targets in human diseases

Expert Opinion on Therapeutic Targets (2007)

Kandiah Jeyaseelan Wishva Herath Arunmozhiarasi Armugam

MicroRNAs (miRNAs) are important endogenous regulators of gene expression. The specific regulation at both the transcription and the translation level (inhibition or mRNA degradation) opens an avenue to use these small RNA molecules as potential targets for the development of novel drugs as well as for the diagnosis of several human diseases. Important information about the role of a miRNA in disease can be deduced by mimicking or inhibiting its activity and examining its impact on the phenotype/behaviour of the cell or organism. Modulating the activity of a miRNA is expected to lead to improvement in disease symptoms and this implies that the target miRNA plays an important role in the disease. It is also now possible to develop miRNA-based therapeutic products that can either increase or decrease the levels of proteins in pathophysiological conditions such as cancer, cardiovascular diseases, viral diseases, metabolic disorders and programmed cell death. The commercial potential of miRNA and related drugs is expected to exponentially increase within the next few years, yet there are several areas in miRNA biology and delivery that need to be extensively investigated.

10.1517/14728222.11.8.1119

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Citations (60)

α-Neurotoxin gene expression in Naja sputatrix: identification of a silencer element in the promoter region

Archives of Biochemistry and Biophysics (2002)

Donghui Ma Arunmozhiarasi Armugam Kandiah Jeyaseelan

Cardiotoxin

Neurotoxin

Silencer

Cobra

10.1016/s0003-9861(02)00245-x

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Citations (10)

Ontogeny of Aquaporins 1 and 3 in Ovine Placenta and Fetal Membranes

Placenta (2000)

H. Johnston Irene Koukoulas Kandiah Jeyaseelan Arunmozhiarasi Armugam Linda Earnest

Amnion

Fetal membrane

Trophoblast

Allantois

Aquaporin 1

Conceptus

10.1053/plac.1999.0445

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Citations (73)

Amino Acid Sequence of Trocarin, a Prothrombin Activator FromTropidechis carinatus Venom: Its Structural Similarity to Coagulation Factor Xa

Jeremiah S. Joseph M.C.M. Chung Kandiah Jeyaseelan R. Manjunatha Kini

Prothrombinase

Factor V

Factor IX

10.1182/blood.v94.2.621

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Citations (81)

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