Alfonso Sanjuánbenito

Instituto Cajal

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Eduardo Lobo

Hospital Universitario Ramón y Cajal

Alfredo Carrato

Instituto Cajal

Julie Earl

Instituto Cajal

Enrique Martínez-Molina

Hospital Universitario Ramón y Cajal

Carmen Guillén‐Ponce

Hospital Universitario Ramón y Cajal

Jaime Ruíz-Tóvar

Comillas Pontifical University

José Montáns

Leiden University Medical Center

Núria Malats

Spanish National Cancer Research Centre

Eduardo Lisa

Instituto Cajal

Enrique Vázquez-Sequeiros

Instituto Ramón y Cajal de Investigación Sanitaria

Cooperative Institutions

Hospital Universitario Ramón y Cajal

155

Instituto Cajal

117

Instituto Ramón y Cajal de Investigación Sanitaria

103

Universidad de Alcalá

Instituto de Salud Carlos III

Centro de Investigación Biomédica en Red

Universidad Autónoma de Madrid

Centro de Investigación Biomédica en Red de Cáncer

Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas

Universitat de Barcelona

Author Statistics

Papers

Citation

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Liver abscess by Eikenella corrodens.

DOAJ (DOAJ: Directory of Open Access Journals) (2015)

Ana Pilar Morante Alfonso Sanjuánbenito Elena Mendía Pietro G. Giordano Francisco García Angarita

Eikenella corrodens

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Citations (2)

Leiomiosarcoma de vena gonadal derecha

Cirugía Española (2020)

Ana Puerta Vicente Alfonso Sanjuánbenito Eduardo Lobo

10.1016/j.ciresp.2020.09.001

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Citations (0)

Combined analysis of a serum mRNA/miRNA marker signature and CA 19‐9 for timely and accurate diagnosis of recurrence after resection of pancreatic ductal adenocarcinoma: A prospective multicenter cohort study

United European Gastroenterology Journal (2024)

Malte Buchholz Ludwig Lausser Miriam Schenk Julie Earl Rita T. Lawlor

Abstract Background and Aims Timely and accurate detection of tumor recurrence in pancreatic ductal adenocarcinoma (PDAC) patients is an urgent and unmet medical need. This study aimed to develop a noninvasive molecular diagnostic procedure for the detection of recurrence after PDAC resection based on quantification of circulating mRNA and miRNA biomarkers in serum samples. Methods In a multicentric study, serum samples from a total of 146 patients were prospectively collected after resection. Samples were classified into a “No Evidence of Disease” and a “Recurrence” group based on clinical follow‐up data. A multianalyte biomarker panel was composed of mRNAs and miRNA markers and simultaneously analyzed in serum samples using custom microfluidic qPCR arrays (TaqMan array cards). A diagnostic algorithm was developed combining a 7‐gene marker signature with CA19‐9 data. Results The best‐performing marker combination achieved 90% diagnostic accuracy in predicting the presence of tumor recurrence (98% sensitivity; 84% specificity), clearly outperforming the singular CA 19‐9 analysis. Moreover, time series data obtained by analyzing successively collected samples from 5 patients during extended follow‐up suggested that molecular diagnosis has the potential to detect recurrence earlier than routine clinical procedures. Conclusions TaqMan array card measurements were found to be biologically valid and technically reproducible. The BioPac multianalyte marker panel is capable of sensitive and accurate detection of recurrence in patients resected for PDAC using a simple blood test. This could allow a closer follow‐up using shorter time intervals than currently used for imaging, thus potentially prompting an earlier work‐up with additional modalities to allow for earlier therapeutic intervention. This study provides a promising approach for improved postoperative monitoring of resected PDAC patients, which is an urgent and unmet clinical need.

TaqMan

10.1002/ueg2.12676

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Citations (0)

Adenocarcinoma primario de intestino delgado

Cirugía Española (2009)

Jaime Ruíz-Tóvar Enrique Martínez-Molina Vicente Morales Alfonso Sanjuánbenito

10.1016/j.ciresp.2008.12.007

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Citations (9)

Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

Journal of Visualized Experiments (2023)

Jesús Frutos Díaz-Alejo Simon April-Monn Marina Cihova Verona Buociková Jorge Villalón López

Tumor organoids are three-dimensional (3D) ex vivo tumor models that recapitulate the biological key features of the original primary tumor tissues. Patient-derived tumor organoids have been used in translational cancer research and can be applied to assess treatment sensitivity and resistance, cell-cell interactions, and tumor cell interactions with the tumor microenvironment. Tumor organoids are complex culture systems that require advanced cell culture techniques and culture media with specific growth factor cocktails and a biological basement membrane that mimics the extracellular environment. The ability to establish primary tumor cultures highly depends on the tissue of origin, the cellularity, and the clinical features of the tumor, such as the tumor grade. Furthermore, tissue sample collection, material quality and quantity, as well as correct biobanking and storage are crucial elements of this procedure. The technical capabilities of the laboratory are also crucial factors to consider. Here, we report a validated SOP/protocol that is technically and economically feasible for the culture of ex vivo tumor organoids from fresh tissue samples of pancreatic adenocarcinoma origin, either from fresh primary resected patient donor tissue or patient-derived xenografts (PDX). The technique described herein can be performed in laboratories with basic tissue culture and mouse facilities and is tailored for wide application in the translational oncology field.

Organoid

Ex vivo

Pancreatic tumor

Primary tumor

Translational Research

10.3791/65229

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Citations (3)

Pancreatic Stent Impact on Duodenopancreatectomy Complications

HPB (2021)

Paula Pastor Peinado Juan Ocaña Paula Muñoz Eduardo Lobo Alfonso Sanjuánbenito

Pancreatic fistula

Pancreatic carcinoma

10.1016/j.hpb.2021.08.868

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Citations (0)

Ascitis quilosa secundaria a pancreatitis: caso clínico y revisión de la bibliografía

Nutrición Hospitalaria (2012)

Jesús M. Gómez‐Martín Enrique Martínez-Molina Alfonso Sanjuánbenito E. Martín-Illana Francisco Arrieta

Chylous ascites is an uncommon finding which is due to the presence of thoracic or intestinal lymph in the abdominal cavity. It is usually caused by a chronic disruption of the lymphatic system. The present report is one of the rare cases in the literature of chylous ascites secondary to idiopathic acute pancreatitis, which showed a complete resolution with a combination of low fat enteral nutrition with MCT and somatostatin analogs.

Chylous Ascites

Abdominal cavity

Chyle

Enteral administration

10.3305/nh.2012.27.1.5481

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Citations (0)

Results of the Surgery of the Pancreatic Metastases of Renal Cancer. Spanish Multi-center Study (Panmekid)

HPB (2021)

Gerardo Blanco‐Fernández Constantino Fondevila-Campo Alfonso Sanjuánbenito Joan Fabregat-Prous F. Rotellar-Sastre

Kidney cancer

10.1016/j.hpb.2021.08.819

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Citations (0)

Relevance of Positive Resection Margins in Ductal Pancreatic Adenocarcinoma and Prognostic Factors

Cirugía Española (English Edition) (2020)

Juan Ocaña Alfonso Sanjuánbenito Alba García José Manuel Molina Eduardo Lisa

Relevance

Pancreatic carcinoma

Resection margin

Margin (machine learning)

10.1016/j.cireng.2020.01.015

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Citations (3)

Circulating tumor cells (CTC) and KRAS mutant circulating free DNA (cfDNA) detection in peripheral blood as biomarkers in patients diagnosed with exocrine pancreatic cancer

BMC Cancer (2015)

Julie Earl Sandra García-Nieto José Carlos Martínez Ávila José Montáns Alfonso Sanjuánbenito

Pancreatic cancer remains one of the most difficult cancers to treat with the poorest prognosis. The key to improving survival rates in this disease is early detection and monitoring of disseminated and residual disease. However, this is hindered due to lack reliable diagnostic and predictive markers which mean that the majority of patients succumb to their condition within a few months.We present a pilot study of the detection circulating free DNA (cfDNA) combined with tumor specific mutation detection by digital PCR as a novel minimally invasive biomarker in pancreatic ductal adenocarcinoma (PDAC). This was compared to the detection of CTC by the CellSearch® system and a novel CTC enrichment strategy based on CD45 positive cell depletion. The aim of the study was to assess tumor specific DNA detection in plasma and CTC detection as prognostic markers in PDAC.We detected KRAS mutant cfDNA in 26% of patients of all stages and this correlated strongly with Overall Survival (OS), 60 days (95% CI: 19-317) for KRAS mutation positive vs 772 days for KRAS mutation negative (95% CI: 416-1127). Although, the presence of CTC detected by the CellSearch® system did correlate significantly with OS, 88 days (95% CI: 27-206) CTC positive vs 393 days CTC negative (95% CI: 284-501), CTC were detected in only 20% of patients, the majority of which had metastatic disease, whereas KRAS mutant cfDNA was detected in patients with both resectable and advanced disease.Tumor specific cfDNA detection and CTC detection are promising markers for the management of patients with PDAC, although there is a need to validate these results in a larger patient cohort and optimize the detection of CTC in PDAC by applying the appropriate markers for their detection.

Circulating tumor cell

Surgical oncology

Liquid biopsy

Minimal Residual Disease

10.1186/s12885-015-1779-7

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Citations (164)