KŠ

Katarína Šoltýs

Comenius University Bratislava

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Miroslav Glasa

Institute of Virology of the Slovak Academy of Sciences

Lukáš Predajňa

Biomedical Research Center of the Slovak Academy of Sciences

Tomáš Szemes

Comenius University Bratislava

Jaroslav Budiš

Comenius University Bratislava

Domenico Pangallo

Slovak Academy of Sciences

Gabriel Minárik

Medirex Group Academy

Nina Sihelská

Biomedical Research Center of the Slovak Academy of Sciences

Lenka Pálková

Veterinary Research Institute

Daniel Mihálik

National Agricultural and Food Centre

Matej Planý

National Agricultural and Food Centre

Cooperative Institutions

Comenius University Bratislava

147

Slovak Academy of Sciences

Biomedical Research Center of the Slovak Academy of Sciences

Slovak University of Technology in Bratislava

University Hospital Bratislava

Institute of Virology of the Slovak Academy of Sciences

Institute of Molecular Biology of the Slovak Academy of Sciences

Czech Academy of Sciences, Institute of Physiology

Geneton (Slovakia)

National Agricultural and Food Centre

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

The tellurite resistance gene cluster of pathogenic bacteria and its effect on oxidative stress response

Folia Microbiologica (2024)

Silvia Vávrová Jozef Grones Katarína Šoltýs Peter Celec Ján Turňa

Abstract Tellurite resistance gene clusters have been identified in numerous pathogenic bacteria, including clinical isolates of Escherichia coli . The rareness of tellurium in host organisms and the noncontaminated environment raises a question about the true functionality of tellurite resistance gene clusters in pathogenesis and their possible contribution to bacterial fitness. The study aims to point out the beneficial effects of the tellurite resistance gene cluster of pathogenic bacteria to survive in ROS-rich environments. Here, we analysed the bacterial response to oxidative stress conditions with and without tellurite resistance gene clusters, which are composed of terWY1XY2Y3 and terZABCDEF genes. By measuring the levels of protein carbonylation, lipid peroxidation, and expression changes of oxidative stress genes upon oxidative stress, we propose a tellurite resistance gene cluster contribution to the elimination of oxidative damage, potentially increasing fitness and resistance to reactive oxygen species during macrophage attack. We have shown a different beneficial effect of various truncated versions of the tellurite resistance gene cluster on cell survival. The terBCDEF genes increased the survival of E. coli strain MC4100 by 13.21%, terW and terZABCDEF by 10.09%, and terWY1XY2Y3 and terZABCDEF by 25.57%, respectively. The ability to survive tellurite treatment is the most significant at 44.8% in wild clinical strain KL53 compared to laboratory strain E. coli MC4100 due to a complete wild-type plasmid presence.

Gene cluster

Pathogenic bacteria

Strain (injury)

10.1007/s12223-024-01133-8

Cite

Citations (0)

Soil Microbiota of Dystric Cambisol in the High Tatra Mountains (Slovakia) after Windthrow

Sustainability (2019)

Alexandra Šimonovičová Lucia Kraková Elena Piecková Matej Planý Mária Globanová

There has been much more damage to forests in the Slovak Republic in the second half of the 20th century than to other European countries. Forested mountain massifs have become a filter of industrial and transportation emissions from abroad, as well as from domestic origins. There are not only acidic deposits of sulphur and heavy metals present in forest soils, but other additional environmental problems, such as climate change, storms, fires, floods, droughts, are worsening the situation. Therefore, forest terrestrial ecosystems are becoming more vulnerable due to changes in natural and environmental conditions. In the High Tatra Mountains in Slovakia, which are protected as a national park, four internationally monitored localities were established after the windthrow disaster in 2004 and fire in 2005: REF, with intact forest; EXT, with extracted wood mass; NEX, with non-extracted wood mass; and FIR, the burnt locality. Soils from these localities were microbiologically analysed with special attention to fungi. Bacterial microbiota detected by high-throughput sequencing showed the prevalence of the genera Acidothermus, Mycobacterium, and Nocardia, and a very low presence of the genera Acidibacter, Burkholderia-Paraburkholderia, Optitus and the uncultured genus Desulfurellaceae H16 in the soil sample from the burnt locality when compared with the unburned sites. Additionally, soil mycocoenoses showed a low similarity between the locality with an intact forest ecosystem and the localities with extracted (REF–EXT) and non-extracted (REF–NEX) wood mass. There was no similarity with the burnt locality (FIR), where heat-resistant fungi dominated. It was shown that the windthrow disaster and subsequent extraction or non-extraction of wood mass did not affect the soil microbial communities or their development. On the other hand, the influence of fire was significant.

Windthrow

Cambisol

10.3390/su11236851

Cite

Citations (8)

Comparison of gut resistomes in healthy individuals and patients with severe alcoholic hepatitis

Acta Chimica Slovaca (2023)

Klára Cverenkárová Petra Olejníková Katarína Šoltýs Lucia Messingerová Ľubomír Skladaný

Abstract Human gut microbiota has been in the centre of scientific interest for a long period of time. Overall health status of an individual has a great impact on the composition of gut microbiota; however, gut microbiota can affect human health. Antibiotic resistance genes (ARGs) are often a part of human gut microbiome. In this paper, total genomic DNA was extracted from stool samples of 147 healthy individuals and of 45 patients with severe alcoholic hepatitis. The presence of six common ARGs ( bla TEM , bla SHV , bla OXA , vanA, tet (A), tet (E)) was analysed in the genomic DNA by end-point PCR. The results show significantly higher occurrence of ARGs in the DNA samples from patients ( p = 0.0001) showing multiple ARGs significantly more often than in healthy individuals ( p = 0.00003). Antibiotic treatment in patients strongly correlated with the occurrence of ARGs ( p = 0.0038). Nutrition and sex of healthy individuals did not have significant effect on the occurrence of ARGs ( p = 0.156; p = 0.456). ARGs’ occurrence in healthy individuals was the highest in the oldest age group, but the age of individuals and ARGs’ occurrence were not related ( p = 0.617). In conclusion, the results underline the importance of health for normal functioning of gut microbiota. Antibiotic resistance represents a challenge in the treatment of patients with liver diseases.

Gut microbiome

10.2478/acs-2023-0011

Cite

Citations (0)

Additional file 1 of Effect of High-intensity Training and Probiotics on Gut Microbiota Diversity in Competitive Swimmers: Randomized Controlled Trial

Figshare (2022)

Viktor Bielik Ivan Hric Simona Ugrayová Libuša Kubáňová Matúš Putala

Additional file 1: Taxonomic profiling of bacterial communities in probiotic Bryndza cheese.

10.6084/m9.figshare.19745312

Cite

Citations (0)

Influence of vitamin D on the expression of mRNA of cytokines in the mucosa of inflammatory bowel disease patients

Bratislavské lekárske listy/Bratislava medical journal (2018)

Zuzana Vrablicova Katarína Šoltýs Anna Krajčovičová Igor Šturdík T. Köller

The aim of this study was to analyze the infl uence of 25(OH)VD serum concentration on the expression of mRNA cytokines (IL-6, IL-8, IL-12, IL-17, IL-23, TNFα, CCR1, CCR2, CCR5, CCR9, CCL5, TLR2, TLR4, TLR5, CD207 ,CD206, FoxP3) in mucosa of IBD patients.The cohort consisted of 86 IBD patients (48 CD and 38 UC) followed at the IBD center of University Hospital Bratislava-Ruzinov.We performed colonoscopy in each patient and took biopsies from mucosa of sigma and terminal ileum.Serum concentration of 25(OH)VD was assessed at the time of colonoscopy.mRNA was extracted from mucosal biopsy samples for each cytokine.Then we analyzed the correlation between VD and the expression of mRNA of cytokines from biopsies samples.In CD we observed a signifi cant positive correlation of serum concentration 25(OH)VD and the expression mRNA level of IL-6.There was also trend towards signifi cant positive correlation of the expression mRNA of TNFα, IL-10, IL-23, TLR 2 in infl amed mucosa of terminal ileum as well as the expression mRNA of CCR5 and CCR1 in non-infl amed mucosa from terminal ileum.We also found a trend towards positive correlation between 25(OH)VD and the expression mRNA of IL-23, TLR4, CD 207, CCR1, CCR5 and CD 206 in non-infl amed mucosa of sigma in UC.VD signifi cantly correlated with the levels of expression of several infl ammatory cytokines including TNFα in colonic mucosa of patients with IBD (Tab.4, Fig. 3, Ref. 31).

Intestinal mucosa

10.4149/bll_2018_074

Cite

Citations (4)

Genetic diversity and intercontinental dispersal of temperate and subarctic populations of Dibothriocephalus dendriticus (Cestoda; Diphyllobothriidea), a causative agent of dibothriocephalosis

International Journal for Parasitology (2025)

Ivica Kráľová-Hromadová Eva Bazsalovicsová Alžbeta Radačovská Katarína Šoltýs Ľudmila Juhásová

The diphyllobothriid tapeworm Dibothriocephalus dendriticus, one of the causative agents of the fish-borne zoonosis dibothriocephalosis, is mainly distributed in the Arctic/subarctic and temperate zones of the Northern Hemisphere (Europe, North America, and Asia), but also in the southern cone region of South America (Patagonia). The genetic structure and gene flow among 589 individuals of D. dendriticus, representing 20 populations, were studied using the mitochondrial cox1 gene as the first choice marker and 10 polymorphic nuclear microsatellite loci as a dominant molecular tool. The haplotype network of 30 cox1 haplotypes and Principal Coordinate Analysis/Structure analysis based on microsatellite data revealed close genetic relationships among populations within continents, namely northern and northwestern Europe (Norway, Finland, and UK/Scotland), North America (USA/Alaska, USA/Oregon, and Greenland), and South America (Argentina and Chile). The population from Iceland, located on the Mid-Atlantic Ridge between North America and Europe, was related to both Europe and North America. The mixed Nearctic and Palaearctic origin of D. dendriticus in Iceland was confirmed by a coalescent-based analysis of the microsatellite loci (in Migrate software). The most likely scenarios suggested that the Icelandic population is a genetic admixture of tapeworms from northwestern Europe and USA/Alaska. These findings corresponded with the distribution and migratory routes of piscivorous birds of the family Laridae, the main definitive hosts of D. dendriticus. The origin of an apparently non-native population of D. dendriticus in Patagonia was investigated in detail. The two most plausible hypotheses based on Migrate analysis represented different scenarios. One of them points to a European origin of the Patagonian population, the other to a North American origin. Future research with more extensive and a geographically broader sampling set is recommended to trace dispersal routes of D. dendriticus in Patagonia.

Subarctic climate

10.1016/j.ijpara.2025.01.002

Cite

Citations (0)

Functional dissection of γ-aminobutyric acid metabolism in Neurospora crassa

General Physiology and Biophysics (2020)

Kamila Ďurišová Martin Šimkovič Ĺudovı́t Varečka Michal Kaliňák Katarína Šoltýs

The GABA shunt is one of the metabolic pathways that is ubiquitous in prokaryotes and eukaryotes.γ-aminobutyric acid (GABA) in fungi is required in the stress responses, virulence and development.The number of genes encoding glutamate decarboxylase (gad), GABA transaminase (gta) and succinic semialdehyde dehydrogenase (ssadh) varies between fungal species.The genome-wide analysis in Neurospora crassa resulted in the identification of a gta and a ssadh.Disruption of either gta or ssadh decreased respiration rate and biomass accumulation, reduced growth on GABA and beta-alanine.The gta and ssadh mutants exhibited aberrant hyphal morphology and displayed differential transcription of the GABA shunt genes.In the gta mutant, protoperithecia and perithecia formation was almost completely suppressed in the presence of GABA and beta-alanine, indicating GTA requirement for the turnover of these amino acids.The strains displayed differential metabolic dysregulations in response to different nitrogen sources.The phenotypic differences between the gta and ssadh mutants could be contributed to accumulation of intermediates of the GABA shunt and/or GABA shunt-independent functions.Together, our data suggest that the GABA shunt could function as a moderate modulator of multiple biological events, including respiration, energy metabolism, carbon and nitrogen metabolism, growth, as well as sexual development in N. crassa.

Crassa

Metabolic pathway

GABA transaminase

10.4149/gpb_2020010

Cite

Citations (2)

Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century

Applied Sciences (2020)

Zuzana Kisová Matej Planý Jelena Pavlović Mária Bučková Andrea Puškárová

A historical crypt offers us a particular view of the conditions of some buried materials (in this case textiles) and the various biogenic phenomena to which they were subjected over the centuries. In addition, significant knowledge can come by studying the DNA of buried objects which allows the recognition of materials, but also to reveal some practice of the funeral ceremony. In this study, the deteriorating microbial communities colonizing various funeral textile items were identified and characterized using microscopic observation, cultivation, polymerase chain reaction (PCR) and sequencing, hydrolytic tests; and culture-independent analysis (high-throughput sequencing, MinION platform). Different PCR assays and consequent sequencing of amplicons were employed to recognize the animal origin of bodice reinforcements and the type of plant used to embellish the young girl. The analysis of ancient DNA (aDNA from animal and plant) was also completed by the application of high-throughput sequencing through Illumina platform. The combination of all these techniques permitted the identification of a complex microbiota composed by dangerous degradative microorganisms able to hydrolyze various organic substrates such as fibroin, keratin, and cellulose. Bacteria responsible for metal corrosion and bio-mineralization, and entomopathogenic and phytopathogenic fungi. The analysis of aDNA identified the animal component used in bodice manufacturing, the plant utilized as ornament and probably the season of this fatal event.

Minion

10.3390/app10165451

Cite

Citations (16)

Seasonal changes of circulating 25-hydroxyvitamin D correlate with the lower gut microbiome composition in inflammatory bowel disease patients

Scientific Reports (2020)

Katarína Šoltýs Martina Stuchlíková Tibor Hlavatý Barbora Gaálová Jaroslav Budiš

Higher probability of the development of Crohn's disease (CD) and ulcerative colitis (UC) as a possible consequence of the north-south gradient has been recently suggested. Living far north or south of the equator is manifested in fluctuation of vitamin D (vitD) levels depending on the season in both healthy and affected individuals. In the present study we investigate the possible link between the seasonal serum vitD level to the microbial composition of the lower gut of Inflammatory Bowel disease (IBD) patients using 16S rRNA sequencing. Decrease of serum vitD level in winter/spring season in a cohort of 35 UC patients and 39 CD patients was confirmed. Low gut microbiota composition of patients with IBD correlated with the serum level of 25(OH)D that directly coupled to seasonal variability of the sunshine in the central European countries. It is supposed to be related to increased abundance of Actinobacteria and Proteobacteria in UC and Actinobacteria, Fusobacteria, Firmicutes and Bacteroidetes in CD. In summer/autumn period, we observed a reduction in abundance of bacterial genera typical for inflammation like Eggerthella lenta, Fusobacterium spp., Bacteroides spp., Collinsella aerofaciens, Helicobacter spp., Rhodococcus spp., Faecalibacterium prausnitzii; and increased abundance of Pediococcus spp. and Clostridium spp. and of Escherichia/Shigella spp.

Faecalibacterium prausnitzii

Fusobacteria

Dysbiosis

10.1038/s41598-020-62811-4

Cite

Citations (33)

Gut diversity and the resistome as biomarkers of febrile neutropenia outcome in paediatric oncology patients undergoing hematopoietic stem cell transplantation

Scientific Reports (2024)

Sára Šardzíková Kristina Andrijkova Peter Švec Gábor Beke Ľuboš Kľúčár

Abstract The gut microbiota of paediatric oncology patients undergoing a conditioning regimen before hematopoietic stem cell transplantation is recently considered to play role in febrile neutropenia. Disruption of commensal microbiota and evolution of opportune pathogens community carrying a plethora of antibiotic-resistance genes play crucial role. However, the impact, predictive role and association of patient´s gut resistome in the course of the therapy is still to be elucidated. We analysed gut microbiota composition and resistome of 18 paediatric oncology patients undergoing hematopoietic stem cell transplantation, including 12 patients developing febrile neutropenia, hospitalized at The Bone Marrow Transplantation Unit of the National Institute of Children´s disease in Slovak Republic and healthy individuals (n = 14). Gut microbiome of stool samples obtained in 3 time points, before hematopoietic stem cell transplantation (n = 16), one week after hematopoietic stem cell transplantation (n = 16) and four weeks after hematopoietic stem cell transplantation (n = 14) was investigated using shotgun metagenome sequencing and bioinformatical analysis. We identified significant decrease in alpha-diversity and nine antibiotic-resistance genes msr(C), dfrG, erm(T), VanHAX, erm(B), aac(6)-aph(2), aph(3)-III, ant(6)-Ia and aac(6)-Ii , one week after hematopoietic stem cell transplantation associated with febrile neutropenia. Multidrug-resistant opportune pathogens of ESKAPE, Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae and Escherichia coli found in the gut carried the significant subset of patient’s resistome. Over 50% of patients treated with trimethoprim/sulfamethoxazole, piperacillin/tazobactam and amikacin carried antibiotic-resistance genes to applied treatment. The alpha diversity and the resistome of gut microbiota one week after hematopoietic stem cell transplantation is relevant predictor of febrile neutropenia outcome after hematopoietic stem cell transplantation. Furthermore, the interindividual diversity of multi-drug resistant opportunistic pathogens with variable portfolios of antibiotic-resistance genes indicates necessity of preventive, personalized approach.

Resistome

10.1038/s41598-024-56242-8

Cite

Citations (3)