Robert F. H. Dekker

Universidade Tecnológica Federal do Paraná

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Aneli M. Barbosa‐Dekker

Universidade Estadual de Londrina

Aneli M. Barbosa

Universidade Estadual de Londrina

Mário Antônio Alves da Cunha

Universidade Tecnológica Federal do Paraná

Ellen Cristine Giese

Centro de Tecnologia Mineral

Eveline Aparecida Isquierdo Fonseca de Queiroz

Universidade Federal de Mato Grosso

Vidiany Aparecida Queiroz Santos

Faculdade de Pato Branco

Maria de Lourdes Corradi da Silva

Universidade Estadual Paulista (Unesp)

Ana Flora Dalberto Vasconcelos

Universidade Estadual Paulista (Unesp)

Gabrielle Cristina Calegari

Universidade Estadual de Londrina

Elen Romão Sartori

Universidade Estadual de Londrina

Cooperative Institutions

Universidade Estadual de Londrina

165

Universidade Tecnológica Federal do Paraná

Universidade de São Paulo

Universidade Estadual Paulista (Unesp)

Universidade Federal do Paraná

Universidade Federal de Mato Grosso

Universidade Estadual de Campinas (UNICAMP)

Lakehead University

Universidade Estadual de Maringá

Universidade Federal do Rio de Janeiro

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Polysaccharide-based substrate for surface-enhanced Raman spectroscopy

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy (2020)

Ingrid Brito Barbosa Aneli M. Barbosa‐Dekker Robert F. H. Dekker Arandi Ginane Bezerra Henrique de Santana

Surface-Enhanced Raman Spectroscopy

Crystal violet

Silver nanoparticle

10.1016/j.saa.2020.119255

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Citations (9)

Influência de Tween na produção de lacases constitutivas e indutivas pelo Botryosphaeria sp

Acta Scientiarum Biological Sciences (2004)

Aneli M. Barbosa‐Dekker Ellen Cristine Giese Luiz Gustavo Covizzi Robert F. H. Dekker

Botryosphaeria sp. is a ligninolytic fungus that produces laccase. It was previously established that veratryl alcohol (VA) strongly induced laccase production in this ascomyceteous. It is well known that surfactants such as Tween - 80 can increase the production of some fungal exo-enzymes. It has been reported that Tween-80 can also induce laccase production in basidiomycetes. In this work, the influence of Tweens 20, 40, 60 and 80 on the production of constitutive laccases (in the absence of VA) and inducible laccases (in the presence of VA) by Botryosphaeria sp. was evaluated, separately. Laccase activity was assayed using two putative laccase substrates: ABTS (PPO - I) and DMP (PPO - II). All of the Tweens examined increased the production of both constitutive and inducible laccase PPO - I, but did not affect the production of constitutive laccase PPO - II. Tweens 60 and 20 increased the production of inducible laccase PPO - II, while Tween 80 and 40 did not cause any effect

ABTS

10.4025/actascibiolsci.v26i4.1536

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Citations (10)

Biotransformation of Kraft lignin by Pleurotus ostreatus Florida in conditions optimized for laccase production

Research Square (Research Square) (2022)

Paula C. I. Franco Igor Shoiti Shiraishi Robert F. H. Dekker Aneli M. Barbosa‐Dekker Dionísio Borsato

Abstract White-rot fungi are efficient degraders of the lignin moiety due to the production of extracellular enzymes that includes the laccases which are able to depolymerize lignin oxidative processes to low-molecular weight phenolic and aromatic compounds. These end-products have important industrial applications, as building blocks for chemical syntheses of high-value. Thus, the objective of this work was to optimize the culture conditions for laccase production by Pleurotus ostreatus Florida grown on Kraft lignin, and to evaluate lignin biodegradation products. The fungal strain produced optimum laccase titers after evaluation in a 3 3 -factorial statistical design by varying: lignin concentration, yeast extract and time of cultivation. The extracellular fluid obtained under the optimized culture condition was treated by liquid-liquid partition with ethyl acetate in order to determine the lignin degradation products by LC-MS/MS. Concentration of lignin was the main variable that increased laccase production assayed against substrate 2,6-dimethoxyphenol. Optimal laccase production predicted by the model was 5.73 U/mL under the condition: 0.87% (w/v) lignin, 4.5% (w/v) yeast extract and 10 days cultivation. The experimental laccase titer obtained following optimal conditions was 5.68 U/mL. The lignin metabolites identified were 4-methoxycinnamaldehyde and cuminaldehyde. In addition, abscisic acid was produced; considered a stress-related hormone product of the fungus. These findings show a suitable source of fungal laccase for obtaining new metabolites from the biodegradation of Kraft lignin by fermentation.

Pleurotus ostreatus

Biotransformation

10.21203/rs.3.rs-1919187/v1

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The Utilization Of Autohydrolysis-Exploded Hardwood (Eucalyptus Regnans) And Sofiwood (Pinus Radiata) Sawdust For The Production Of Cellulolytic Enzymes And Fermentable Substrates

Biocatalysis (1987)

Robert F. H. Dekker

Softwood (Pinus radiata) and hardwood (Eucalyptus regnans) sawdust were pretreated by auto-hydrolysis at 200°C followed by explosive decompression. P. radiata pretreated pulps were poorly hydrolyzed by Trichoderma reesei C-30 cellulases compared to E. regnans pulps. Impregnation of P. radiata with 4.44% (w/w) SO2 followed by autohydrolysis explosion resulted in increased enzymic saccharification of the pulps. Sulfur dioxide treatment resulted in more of the hemicellulose components (heteroxylan and glucomannan) being removed during autohydrolysis than when P. radiata was pretreated by autohydrolysis alone. Trichoderma reesei C-30 cellulase digests containing 10% (w/v) of the pretreated pulps and 20 FPU units/g pulp resulted in cellulose-to-glucose conversion yields of 32% with E. regnans, 16% with P. radiata and 27% with SO2-treated P. radiata within 24 h at 50°C (pH 5.0) and could be increased to 74%, 27% and 48%, respectively, when the cellulase digests were supplemented with an exogenous β-glucosidase (Novozym 188). Cellulase, β-glucosidase and xylanase production by T. reesei C-30 grown in shake culture (25°C, pH 5.0) on 1% (w/v) of the pretreated pulps was highest when E. regnans and SO2-treated P. radiata were used as growth substrates, and enzyme yields compared with those when a purified α-cellulose (Solka floc) was used as growth substrate. Autohydrolysis-exploded P. radiata, however, proved to be a rather poor substrate for enzyme production. Typical FPU yields (as IU/g cellulose) were 53, P. radiata; 126, SO2-treated P. radiata; 136, Solka floc; and 165, E. regnans.

Pinus radiata

Radiata

Softwood

Sawdust

Enzymatic Hydrolysis

10.3109/10242428709040131

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Citations (21)

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

Semina Ciências Exatas e Tecnológicas (2010)

Anelize Bauermeister Maria Inês Rezende Ellen Cristine Giese Robert F. H. Dekker Aneli M. Barbosa‐Dekker

beta-1,3-Glucanases sao enzimas que hidrolisam ligacoes glicosidicas do tipo beta-1,3 presentes em beta-D-glucanas, liberando glucose como produto principal. Estas enzimas sao produzidas por fungos filamentosos e leveduriformes, assim como bacterias, apresentando amplas aplicacoes biotecnologicas. O objetivo do presente trabalho foi revisar a literatura publicada sobre beta-1,3-glucanases fungicas, enfatizando-se suas funcoes nos micro-organismos, alguns parâmetros utilizados para a determinacao da atividade enzimatica, substratos utilizados e condicoes de cultivo para a sua producao. Tambem foram relatadas aplicacoes destas enzimas na obtencao de oligossacarideos bioativos, na caracterizacao da parede celular de micro-organismos, no controle biologico de patogenos de algumas plantas, como aditivos em racoes para animais, alem de sua adicao em vinhos para melhorar as caracteristicas organolepticas.

10.5433/1679-0375.2010v31n2p75

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Citations (18)

Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil

Orbital - The Electronic Journal of Chemistry (2018)

João Paulo Sacchetto Arnaldo F. Silva Maria Inês Rezende Ieda Spacino Scarmínio Marco Antônio Záchia Ayub

Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorganic nitrogen (NH 4 Cl, NaNO 3 , (NH 4 ) 2 SO 4 , and NH 4 NO 3 ), organic nitrogen (yeast extract (YE), urea, peptone, and corn steep liquor) sources, inorganic phosphate (KH 2 PO 4 , Na 2 HPO 4 , NaH 2 PO 4 , K 2 HPO 4 ), as well as increasing concentrations of copper (from zero to 100 µg mL -1 ), as nutritional requirements for enhancing laccase production by B. rhodina MAMB-05 were also evaluated. Soybean oil was the only carbon source in a central composite factorialdesign (2 3 ) to optimize laccase production with the selected variables NaNO 3 , KH 2 PO 4 and copper (CuSO 4 .5H 2 O), using the response surface methodology. The optimal conditions obtained were NaNO 3 (100 g L -1 ), KH 2 PO 4 (125 g L -1 ) and 50 µg mL -1 of CuSO 4 .5H 2 O. The specific laccase activity obtained (2.95 U mg -1 ) in the validation experiments using the optimal condition for laccase production by B. rhodina MAMB-05 was in agreement with the predicted value (3.07 U mg -1 ), and demonstrated the fitness of the proposed model. DOI: http://dx.doi.org/10.17807/orbital.v10i7.1206

Botryosphaeria

Carbon source

Soybean oil

10.17807/orbital.v10i7.1206

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Citations (1)

Fungal beta-1,3-Glucanases: production and biotechnological applications.

DOAJ (DOAJ: Directory of Open Access Journals) (2010)

Robert F. H. Dekker Ellen Cristine Giese Maria Inês Rezende Anelize Bauermeister Aneli M. Barbosa‐Dekker

BETA (programming language)

Source

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Citations (2)

Functional Food Supplement Enriched with Vegetable Proteins and Probiotics: A Hyperproteic and Probiotic-Formulated Product

Eliane Colla Daiane Meneguzzi Thaís Fernandes Mendonça Mota Joyce da Cruz Ferraz Dutra Solange Teresinha Carpes

Functional foods enriched with probiotics can contribute to maintaining health and improving mental and cognitive states. This research involved developing and characterizing a hyperproteic dietary supplement containing a blend of plant proteins and probiotic cells (Lactobacillus reuteri LRE 02 ID 1774). The supplement exhibited high nutritional quality, particularly in protein content and amino acid profile, containing all essential amino acids and branched-chain amino acids (BCAA). Omega-3 fatty acids (150 mg/100g), omega-6 (1420 mg/100g), and omega-9 (1180 mg/100g, cis-11-eicosenoic acid 20 mg/100g) were also detected. The supplement is a source of dietary fiber and probiotics (108 CFU/mL) and demonstrates moderate antioxidant activity. It can be considered innovative as it contains essential amino acids and oryzatein, an immuno-stimulating peptide. Its relevance lies in its hypoallergenic properties, low concentration of anti-nutritional factors, good digestibility, and being gluten- and lactose-free. Additionally, it includes Lactobacillus reuteri, a probiotic with potential immunomodulatory and neuro-cognitive benefits.

Functional food

Vegetable Proteins

10.20944/preprints202411.1169.v1

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Enzymatic hydrolysis of botryosphaeran and laminarin by β-1,3-glucanases produced by Botryosphaeria rhodina and Trichoderma harzianum Rifai

Process Biochemistry (2006)

Ellen Cristine Giese Luiz Gustavo Covizzi Robert F. H. Dekker Nilson K. Monteiro Maria de Lourdes Corradi da Silva

Laminarin

Trichoderma harzianum

Enzymatic Hydrolysis

Glucanase