Zuohua Zhu

Chinese Academy of Agricultural Sciences

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Chunliang Xie

Chinese Academy of Agricultural Sciences

Yuande Peng

Chang Gung Memorial Hospital

Wenbing Gong

Institute of Bast Fiber Crops

Yingjun Zhou

Central South University

Zhenxiu Hu

Institute of Bast Fiber Crops

Yan Li

Nanjing Tech University

Chao Xu

Guangdong University of Technology

Cha Cao

Institute of Bast Fiber Crops

Yuande Peng

Institute of Bast Fiber Crops

Yingjun Zhou

Fudan University

Cooperative Institutions

Seascape Consultants (United Kingdom)

263

Caterpillar (United States)

256

Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Pfizer (United Kingdom)

Institute of Bast Fiber Crops

University of Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Ministry of Agriculture and Rural Affairs

Kyiv City Clinical Oncology Center

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Solid-state fermentation of Apocynum venetum L. by Aspergillus niger: Effect on phenolic compounds, antioxidant activities and metabolic syndrome-associated enzymes

Frontiers in Nutrition (2023)

Cha Cao Dengfan Lin Yingjun Zhou Na Li Yiwen Wang

This study aimed to evaluate the effect of solid-state fermentation (SSF) with Aspergillus niger on the total phenolic content (TPC), the total flavonoid content (TFC), individual phenolic contents, and antioxidant and inhibitory activities against metabolic syndrome-associated enzymes in an ethanol extract from Apocynum venetum L. (AVL). TPC, TFC, and the contents of quercetin and kaempferol during SSF were 1.52-, 1.33-, 3.64-, and 2.22-fold higher than those of native AVL in the ethyl acetate (EA) subfraction of the ethanol extract. The ABTS·+, DPPH· scavenging, and inhibitory activities against α-glucosidase and pancreatic lipase were found to be highest in the EA subfraction. Fermentation significantly increased the ABTS radical cation, DPPH radical scavenging, and pancreatic lipase inhibitory activities by 1.33, 1.39, and 1.28 times, respectively. TPC showed a significantly positive correlation with antioxidant activities or inhibition against metabolic syndrome-associated enzymes. This study provides a theoretical basis for producing tea products with enhanced antioxidant, antidiabetic, and antihyperlipidemic activities.

ABTS

Solid-State Fermentation

Aspergillus niger

10.3389/fnut.2023.1125746

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Citations (8)

Whole genome sequence of an edible and medicinal mushroom, Hericium erinaceus (Basidiomycota, Fungi)

Genomics (2020)

Wenbing Gong Yahui Wang Chunliang Xie Yingjun Zhou Zuohua Zhu

Hericium erinaceus

Agaricales

Erinaceus

Edible mushroom

Pleurotus

Basidiocarp

10.1016/j.ygeno.2020.01.011

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Citations (51)

Comparative secretome of white‐rot fungi reveals co‐regulated carbohydrate‐active enzymes associated with selective ligninolysis of ramie stalks

Microbial Biotechnology (2020)

Chunliang Xie Wenbing Gong Zuohua Zhu Yingjun Zhou Chao Xu

Summary In the present research, Phanerochaete chrysosporium and Irpex Lacteus simultaneously degraded lignin and cellulose in ramie stalks, whereas Pleurotus ostreatus and Pleurotus eryngii could depolymerize lignin but little cellulose. Comparative proteomic analysis of these four white‐rot fungi was used to investigate the molecular mechanism of this selective ligninolysis. 292 proteins, including CAZymes, sugar transporters, cytochrome P450, proteases, phosphatases and proteins with other function, were successfully identified. A total of 58 CAZyme proteins were differentially expressed, and at the same time, oxidoreductases participated in lignin degradation were expressed at higher levels in P. eryngii and P. ostreatus . Enzyme activity results indicated that cellulase activities were higher in P. chrysosporium and I. lacteus , while the activities of lignin‐degrading enzymes were higher in P. eryngii and P. ostreatus . In addition to the lignocellulosic degrading enzymes, several proteins including sugar transporters, cytochrome P450 monooxygenases, peptidases, proteinases, phosphatases and kinases were also found to be differentially expressed among these four species of white‐rot fungi. In summary, the protein expression patterns of P. eryngii and P. ostreatus exhibit co‐upregulated oxidoreductase potential and co‐downregulated cellulolytic capability relative to those of P. chrysosporium and I. lacteus , providing a mechanism consistent with selective ligninolysis by P. eryngii and P. ostreatus .

Phanerochaete

Chrysosporium

Pleurotus ostreatus

Pleurotus eryngii

Beta-glucosidase

10.1111/1751-7915.13647

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Citations (21)

Probiotic assessment and antioxidant characterization of Lactobacillus plantarum GXL94 isolated from fermented chili

Frontiers in Microbiology (2022)

Yingjun Zhou Wenbing Gong Chao Xu Zuohua Zhu Yuande Peng

Oxidative stress is caused by an imbalance between prooxidants and antioxidants, which is the cause of various chronic human diseases. Lactic acid bacteria (LAB) have been considered as an effective antioxidant to alleviate oxidative stress in the host. To obtain bacterium resources with good antioxidant properties, in the present study, 113 LAB strains were isolated from 24 spontaneously fermented chili samples and screened by tolerance to hydrogen peroxide (H2O2). Among them, Lactobacillus plantarum GXL94 showed the best antioxidant characteristics and the in vitro antioxidant activities of this strain was evaluated extensively. The results showed that L. plantarum GXL94 can tolerate hydrogen peroxide up to 22 mM, and it could normally grow in MRS with 5 mM H2O2. Its fermentate (fermented supernatant, intact cell and cell-free extract) also had strong reducing capacities and various free radical scavenging capacities. Meanwhile, eight antioxidant-related genes were found to up-regulate with varying degrees under H2O2 challenge. Furthermore, we evaluated the probiotic properties by using in vitro assessment. It was showed that GXL94 could maintain a high survival rate at pH 2.5% or 2% bile salt or 8.0% NaCl, live through simulated gastrointestinal tract (GIT) to colonizing the GIT of host, and also show higher abilities of auto-aggregation and hydrophobicity. Additionally, the usual antibiotic susceptible profile and non-hemolytic activity indicated the safety of the strain. In conclusion, this study demonstrated that L. plantarum GXL94 could be a potential probiotic candidate for producing functional foods with antioxidant properties.

10.3389/fmicb.2022.997940

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Citations (30)

Biodegradation of ramie stalk by Flammulina velutipes: mushroom production and substrate utilization

AMB Express (2017)

Chunliang Xie Wenbing Gong Yan Li Zuohua Zhu Zhenxiu Hu

In the textile industry, ramie stalk is byproducts with a low economic value. The potential use of this leftover as a substrate ingredient for Flammulina velutipes (F. velutipe) cultivation was evaluated. The degradation and utilization of ramie stalk by F. velutipes was evaluated through mushroom production, lignocelluloses degradation and lignocellulolytic enzymes activity. The best substrate mixture for F. velutipes cultivation comprised 50% ramie stalk, 20% cottonseed hulls, 25% wheat bran, 4% cornstarch and 2% CaCO3. The highest biological efficiency of fruiting bodies was reached 119.7%. F. velutipes appears to degrade 12.7-32.0% lignin, 14.4-30.2% cellulose and 9.3-25.7% hemicellulose during cultivation on the different substrates. The results of enzymes activities showed that laccase and peroxidase were higher before fruiting; while cellulase and hemicellulase showed higher activities after fruiting. The biological efficiency of fruiting bodies was positively correlated with the activities of cellulase, hemicellulase and ligninolytic enzyme. The results of this study demonstrate that ramie stalk can be used as an effective supplement for increasing mushroom yield in F. velutipes.

Flammulina

Stalk

Ramie

10.1186/s13568-017-0480-4

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Citations (52)

Improving saccharification of ramie stalks by synergistic effect of in-house cellulolytic enzymes consortium

AMB Express (2022)

Cha Cao Zuohua Zhu Chao Xu Wenbing Gong Yingjun Zhou

The high cost of cellulase is one of the main obstacles hindering the large-scale biorefining of lignocellulosic biomass. Therefore, developing efficient method for preparation of cellulase is promising. In the present study, the production of cellulase by Trichoderma reesei, Trichoderma harzianum, and Aspergillus niger was optimized, and the synergistic effect of these cellulase on enzymatic hydrolysis of pretreated ramie stalks was also evaluated. The maximum CMCase (Carboxymethyl Cellulase) and filter paper activity (FPA) produced by T. reesei reached to 3.12 IU/mL and 0.13 IU/mL, respectively. The maximum activities of CMCase (3.68 IU/mL), FPA (0.04 IU/mL) and β-glucosidase (8.44 IU/mL) were obtained from A. niger. The results also showed that under the premise of the same FPA activity, the contribution of β-glucosidase activity to yield of reducing sugar was greater than that of CMCase. Besides, cellulase produced by T. reesei and A. niger had the best synergistic effect on enzymatic hydrolysis of pretreated ramie stalks. The highest reducing sugars yield (417 mg/g dry substrate) was achieved when enzyme cocktail was prepared at the ratio of 1:1, which was 1.36-3.35 folds higher than that of different single enzymes. The present research has provided a novel method for efficient preparation of enzymes consortium for enzymatic hydrolysis of ramie stalks.

Ramie

Aspergillus niger

Trichoderma harzianum

Enzymatic Hydrolysis

10.1186/s13568-022-01453-3

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Citations (2)

Mapping the Secretome and Its N-Linked Glycosylation of Pleurotus eryngii and Pleurotus ostreatus Grown on Hemp Stalks

Journal of Agricultural and Food Chemistry (2019)

Chunliang Xie Wenbing Gong Zuohua Zhu Yingjun Zhou Yan Li

Our previous research showed that Pleurotus eryngii and Pleurotus ostreatus were effective fungi for pretreatment of industrial hemp stalks to improve enzymatic saccharification. The secretomes of these two fungi were analyzed to search for the effective enzyme cocktails degrading hemp lignin during the pretreatment process. In total, 169 and 155 proteins were identified in Pleurotus eryngii and Pleurotus ostreatus, respectively, and 50% of the proteins involved in lignocellulose degradation were CAZymes. Because most of the extracellular proteins secreted by fungi are glycosylated proteins, the N-linked glycosylation of enzymes could be mapped. In total, 27 and 24 N-glycosylated peptides were detected in Pleurotus eryngii and Pleurotus ostreatus secretomes, respectively. N-Glycosylated peptides of laccase, GH92, exoglucanase, phenol oxidase, α-galactosidase, carboxylic ester hydrolase, and pectin lyase were identified. Deglycosylation could decrease enzymatic saccharification of hemp stalks. The activities of laccase, α-galactosidase, and phenol oxidase and the thermal stability of laccase were reduced after deglycosylation.

Pleurotus ostreatus

Pleurotus eryngii

Pleurotus

Beta-glucosidase

Flammulina

10.1021/acs.jafc.9b00061

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Citations (4)

Mapping the metabolic signatures of fermentation broth, mycelium, fruiting body and spores powder from Ganoderma lucidum by untargeted metabolomics

LWT (2020)

Chunliang Xie Shaowei Yan Zhoumei Zhang Wenbing Gong Zuohua Zhu

10.1016/j.lwt.2020.109494

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Citations (28)

Lactiplantibacillus plantarum AR113 Exhibit Accelerated Liver Regeneration by Regulating Gut Microbiota and Plasma Glycerophospholipid

Frontiers in Microbiology (2022)

Chunliang Xie Zhoumei Zhang Manyi Yang Cha Cao Yingjun Zhou

Emerging evidence indicates that probiotics have been proved to influence liver injury and regeneration. In the present study, the effects of Lactiplantibacillus plantarum AR113 on the liver regeneration were investigated in 70% partial hepatectomy (PHx) rats. Sprague-Dawley (SD) rats were gavaged with L. plantarum AR113 suspensions (1 × 1010 CFU/mL) both before and after partial hepatectomy. The results showed that L. plantarum AR113 administration 2 weeks before partial hepatectomy can accelerate liver regeneration by increased hepatocyte proliferation and tumor necrosis factor-α (TNF-α), hepatocyte growth factor (HGF), and transforming growth factor-β (TGF-β) expression. Probiotic administration enriched Lactobacillus and Bacteroides and depleted Flavonifractor and Acetatifactor in the gut microbiome. Meanwhile, L. plantarum AR113 showed decline of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidyl serine (PS), and lysophosphatidyl choline (LysoPC) levels in the serum of the rats after the L. plantarum AR113 administration. Moreover, L. plantarum AR113 treated rats exhibited higher concentrations of L-leucine, L-isoleucine, mevalonic acid, and lower 7-oxo-8-amino-nonanoic acid in plasma than that in PHx. Spearman correlation analysis revealed a significant correlation between changes in gut microbiota composition and glycerophospholipid. These results indicate that L. plantarum AR113 is promising for accelerating liver regeneration and provide new insights regarding the correlations among the microbiome, the metabolome, and liver regeneration.

Glycerophospholipids

10.3389/fmicb.2021.800470

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Citations (18)

Comparative transcriptomics of Pleurotus eryngii reveals blue-light regulation of carbohydrate-active enzymes (CAZymes) expression at primordium differentiated into fruiting body stage

Genomics (2017)

Chunliang Xie Wenbing Gong Zuohua Zhu Yan Li Zhenxiu Hu

Primordium

Pleurotus eryngii

KEGG

10.1016/j.ygeno.2017.09.012

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Citations (64)