ÂA

Ângela Alves

Universidade do Porto

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Elsa Oliveira

Universidade do Porto

Alexandre Lobo‐da‐Cunha

Universidade do Porto

Mário Sousa

Universidade do Porto

Graça Casal

Cooperativa de Ensino Superior Politécnico e Universitário

Gonçalo Calado

Universidade Lusófona

Carlos Azevedo

Universidade do Porto

Rosália Sá

Universidade do Porto

Sónia Rocha

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Marlene Costa

Universidade do Porto

Alberto Barros

Centro de Genética Clínica

Cooperative Institutions

Universidade do Porto

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Centro de Genética Clínica

Institute of Biomedical Science

Universidade Federal Rural de Pernambuco

Centro Hospitalar do Porto

Centro Interdisciplinar de Investigação Marinha e Ambiental

Rede de Química e Tecnologia

University of Lisbon

University of Minho

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Ultrastructural and histochemical study of the digestive gland in cephalaspideans (Mollusca, Euopisthobranchia)

Microscopy and Microanalysis (2015)

Alexandre Lobo‐da‐Cunha Elsa Oliveira Ângela Alves Gonçalo Calado

10.1017/s1431927614013749

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Citations (1)

Mannitol oxidase and polyol dehydrogenases in the digestive gland of gastropods: Correlations with phylogeny and diet

PLoS ONE (2018)

Alexandre Lobo‐da‐Cunha Diogo Amaral-de-Carvalho Elsa Oliveira Ângela Alves Vítor Costa

Mannitol oxidase and polyol dehydrogenases are enzymes that convert polyalcohols into sugars. Mannitol oxidase was previously investigated in terrestrial snails and slugs, being also present in a few aquatic gastropods. However, the overall distribution of this enzyme in the Gastropoda was not known. Polyol dehydrogenases are also poorly studied in gastropods and other mollusks. In this study, polyalcohol oxidase and dehydrogenase activities were assayed in the digestive gland of 26 species of gastropods, representing the clades Patellogastropoda, Neritimorpha, Vetigastropoda, Caenogastropoda and Heterobranchia. Marine, freshwater and terrestrial species, including herbivores and carnivores were analyzed. Ultrastructural observations were undertake in species possessing mannitol oxidase, in order to investigate the correlation between this enzyme and the presence of tubular structures known to be associated with it. Mannitol oxidase activity was detected in the digestive gland of herbivores from the clades Caenogastropoda and Heterobranchia, but not in any carnivores or in herbivores from the clades Patellogastropoda, Neritimorpha and Vetigastropoda. In most of the species used in this study, dehydrogenase activities were detected using both D-mannitol and D-sorbitol as substrates. Nevertheless, in some carnivores these activities were not detected with both polyalcohols. Ultrastructural observations revealed tubular structures in digestive gland cells of some species having mannitol oxidase activity, but they were not observed in others. Based on our results, we suggest that mannitol oxidase first occurred in a herbivorous or omnivorous ancestor of Apogastropoda, the clade formed by caenogastropods and heterobranchs, being subsequently lost in those species that shifted towards a carnivorous diet.

Opisthobranchia

Nudibranch

10.1371/journal.pone.0193078

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O SUPPLY CHAIN SOBRE O OLHAR DOS ESTUDANTES DE ADMINISTRAÇÃO DA ESCOLA TÉCNICA PERNAMBUCANA UNIDADE GOIANA-PE.

J. Oliveira Guilherme da Silva Andrade Ângela Alves

O artigo exibido a seguir discorrerá sobre o Supply Chain na visão dos estudantes do curso técnico de Administração da Escola Técnica Pernambucana da Unidade Goiana-PE com o estudo de caso “Cheiro no cangote” que viabiliza a fomentação da apresentação de uma solução para o caso com a aplicação dessa Cadeia de Abastecimento de forma eficaz. Refletindo que os estudantes estão sendo preparados pela instituição para o mercado de trabalho, o Professor ao aplicar o estudo de caso os coloca no papel de gestor e numa posição de tomada de decisão frente ao problema em questão e assim aplicar o Supply Chain que é a Cadeia de Abastecimento para ganhar vantagem competitiva e ter efeciência nessa logística que indubitavelmente acarretará em cliente satisfeito e valorizado, haja vista que esse deve ser o objetivo da empresa. O Supply Chain quando implantado de forma certa na organização é capaz de reduzir custos, aumentar a produção, satisfazer os clientes e ganhar grandes vantagens comparadas às empresas que não a utiliza ou usa de forma errônea. O trabalho de estudo de caso não possui o objetivo unicamente de exercitar os estudantes, mas também de simular uma realidade futura para que se tornem administradores diferenciados e com visão de mudança através dessa ferramenta, bem como de fazer valer os ensinamentos da disciplina e assim entregar ao mercado de trabalho, jovens adultos preparados para contribuir com a empresa no seu crescimento por meio de uma tão eficiente ferramenta. O momento em que os estudantes tiveram acesso ao estudo de caso foi logo pós uma aula sobre o supracitado assunto em seguida, ao concluir a atividade houve um debate sobre as diferentes opinões e respostas o que oportunizou outra bbemfeitoria, a escuta. Cada um ouviu a solução que os colegas apresentaram para o problema de estudo de caso e puderam notar as diferentes interpretações e diversas maneiras de aplicar o Supply Chain na empresa como solucionadora e mola de impulso para o crescimento da organização. O estudo de caso “Cheiro no cangote” foi aplicado aos estudantes de curso técnico de Administração da ETP Unidade Goiana-PE pelo Professor Alisson Oliveira e com o apoio da Coordenação do curso. O intuito da proposta foi alcançado com sucesso já que os resultados apontaram a compreensão do problema e a amostra adequada de soluções, desta forma, os estudantes demonstraram capacidade para atuar na cadeia de abastecimento de toda e qualquer empresa. Palavras-Chave: Supplay Chain, Administração, Estudantes, Competitivade.

10.31692/ijmpdvg.v2i1.27

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Cryopreservation of eucalyptus genetic resources.

PubMed (2014)

Ergün Kaya Ângela Alves L. Rodrigues Maria M. Jenderek M. Hernandez-Ellis

The long-term preservation of forest genetic resources is a vital part of preserving our forest crops for future generations. Unfortunately, there are few genebanks dedicated to forest trees and very few methods for long-term preservation of forest genetic resources collections aside from field plantings of a limited number of seed-derived or elite clonal individuals. The use of cryopreservation for the long-term storage of elite germplasm is increasingly being used for the long-term preservation of clonal agronomic crops but for forest trees, such as Eucalyptus, the methodology for cryopreservation of diverse genetic resources collections has not been established. We report the successful cryopreservation of a germplasm collection of in vitro shoot cultures of thirteen Eucalyptus spp. lines consisting of two E. grandis x E. camaldulensis lines, seven E. urophylla x E. grandis lines, one E. grandis line, two E. grandis x E. urophylla lines, and one E. camaldulensis line. In a comparison of two cryopreservation methods, sucrose sensitivity limited the application of encapsulation-dehydration. However, with droplet-vitrification, all thirteen lines had good survival after cryopreservation in liquid nitrogen. A 30 min exposure to Plant Vitrification Solution 2 (PVS2) yielded post-liquid nitrogen survival between 38% and 85% depending on the line. One hundred shoot tips from all thirteen lines are currently in long-term storage as a germplasm collection.

Germ plasm

Eucalyptus camaldulensis

Vitrification

Genetic Resources

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Citations (18)

Light and electron microscopy study of the digestive system in the Euopisthobranchia (Mollusca, Gastropoda)

Microscopy and Microanalysis (2015)

Alexandre Lobo‐da‐Cunha Elsa Oliveira Ângela Alves Gonçalo Calado

10.1017/s1431927614013725

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Stereological study of organelle distribution in human oocytes at metaphase I

Zygote (2020)

Sofia Coelho A. Luís Elsa Oliveira Ângela Alves Carla Leal

Summary We have previously presented a stereological analysis of organelle distribution in human prophase I oocytes. In the present study, using a similar stereological approach, we quantified the distribution of organelles in human metaphase I (MI) oocytes also retrieved after ovarian stimulation. Five MI oocytes were processed for transmission electron microscopy and a classical manual stereological technique based on point-counting with an adequate stereological grid was used. Kruskal–Wallis and Mann–Whitney U -tests with Bonferroni correction were used to compare the means of relative volumes (Vv) occupied by organelles. In all oocyte regions, the most abundant organelles were mitochondria and smooth endoplasmic reticulum (SER) elements. No significant differences were observed in Vv of mitochondria, dictyosomes, lysosomes, or SER small and medium vesicles, tubular aggregates and tubules. Significant differences were observed in other organelle distributions: cortical vesicles presented a higher Vv ( P = 0.004) in the cortex than in the subcortex (0.96% vs 0.1%) or inner cytoplasm (0.96% vs 0.1%), vesicles with dense granular contents had a higher Vv ( P = 0.005) in the cortex than in the subcortex (0.1% vs 0%), and SER large vesicles exhibited a higher Vv ( P = 0.011) in the inner cytoplasm than in the subcortex (0.2% vs 0%). Future stereological analysis of metaphase II oocytes and a combined quantitative data of mature and immature oocytes, will enable a better understanding of oocyte organelle distribution during in vivo maturation. Combined with molecular approaches, this may help improve stimulation protocols and in vitro maturation methods.

Organelle

Stereology

10.1017/s0967199420000131

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Impacto ambiental de variedade de mandioca resistente à podridão radicular no Estado de Sergipe.

C. O. de Almeida Ângela Alves

O artigo apresenta os resultados da avaliacao de Impacto Ambiental da variedade BRS Kiriris realizada em 32 estabelecimentos rurais dos municipios de Campo do Brito, Lagarto, Sao Domingos e Simao Dias localizados na mesorregiao do Agreste Sergipano. A avaliacao de impacto ambiental foi realizada com o uso da ferramenta Sistema Ambitec, elaborada pela Embrapa Meio Ambiente, a qual engloba aspectos e indicadores ambientais amplamente utilizados na literatura especializada. O indice geral de impacto ambiental da variedade BRS Kiriris foi positivo (0,42), entretanto, bastante baixo, tendo em vista a escala considerada no sistema Ambitec, que pode variar de +15 a -15. Apenas o indicador ?Uso de recursos naturais? foi positivo, em virtude do incremento de produtividade proporcionado pela variedade. Os indicadores ?Uso de agroquimicos?, ?Uso de energia? e ?Biodiversidade? apresentaram coeficientes negativos, devido as elevacoes no uso de fertilizantes no primeiro caso, de diesel e lenha/carvao vegetal no segundo caso e de perdas de variedades caboclas no ultimo. Os demais indicadores (?Atmosfera?, ?Qualidade do solo?, ?Qualidade da agua? e ?Recuperacao ambiental?) nao foram afetados pela introducao da variedade BRS Kiriris.

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Historical and ultrastructural characterisation of the stomach and intertine of th opisthobranch "Bulla striata" (heterobranchia: cephalaspidea)

Thalassas An International Journal of Marine Sciences (2011)

Alexandre Lobo da Cunha Ana Rita Malheiro Ângela Alves Elsa Oliveira Rita S. Coelho

Bulla (seal)

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L. sericeus collected in S. Tome.

Ângela Alves Luís Prista

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Ultrastructure of two microsporidians Inodosporus sp. and Myospora sp. co-infecting muscles of the Amazon River prawn Macrobrachium amazonicum (Heller, 1862)

Nauplius (2021)

Lucas Rezende Penido Paschoal Fernando José Zara Sónia Rocha Ângela Alves Graça Casal

In the present study, we describe a co-infection of two microsporidians within the abdominal muscle of the Amazon River prawn Macrobrachium amazonicum from Brazil, detected through light and transmission electron microscopy and histopathological analysis. Two spore types and respective early developmental stages (meronts, sporonts, and sporoblasts) appeared grouped in numerous adjacent clusters among the muscle fibers of the host. Spores were initially divided into two morphotypes, Sp. 1 and Sp. 2, based on differences in shape, size, and internal organization. The Sp. 1 spores are pyriform to ovoid (4.3 ± 0.3 × 3.7 ± 0.4 µm in size) and resided in groups of eight within a sporophorous vesicle. Sp. 1 spores had bilayered walls and long tape-like external filaments with irregular morphology and size, forming a complex contiguous membranous system attached to the spore wall. The umbrella-like anchoring disc of the spores was in continuity with an anisofilar polar tube arranged in 7‒8 (rarely 9) coils. The Sp. 2 spores were rod-like in shape (3.1 ± 0.5 × 0.8 ± 0.1 µm in diameter) and the polar tube had 7‒8 coils. Their merogonic and sporogonic stages occurred within the sarcoplasm of the muscle cells in close contact with the myofibrils. Based on ultrastructural organization of the sporogonic stages, the Sp. 1 and Sp. 2 morphotypes probably belong to the genera Inodosporus and Myospora, respectively.

Polar filament

10.1590/2358-2936e2021046

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Citations (2)