Pekka Maijala

Seinäjoki University of Applied Sciences

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Detection of extracellular cellulolytic and proteolytic activity in ectomycorrhizal fungi and Heterobasidion annosum (Fr.) Bref.

New Phytologist (1991)

Pekka Maijala Kurt Fagerstedt Marjatta Raudaskoski

summary Proteolytic and cellulolytic activities were studied in the ectomycorrhizal fungi Amanita regalis (Fr.) Maire, Paxillus involutus (Batsch ex Fr.) Fr. and Suillus bovinus (L. ex Fr.) O. Kuntze and the necrotrophic root pathogenic fungus Heterobasidion annosum (Fr.) Bref. using plate assays, native gel electrophoresis and substrate gel replicas. All the fungi produced carboxymethyl cellulose (CMC)‐hydrolysing activity. Two CMCase bands were detected in H. annosum and P. involutus and one in A. regalis and S. bovinus. In the plate assays, extracellular proteolytic activity was found in all the fungi and the activities were visualized in H. annosum and A. regalis after native polyacrylamide gel electrophoresis.

Paxillus involutus

Heterobasidion annosum

Ectomycorrhiza

10.1111/j.1469-8137.1991.tb00968.x

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Citations (47)

Lignin Model Compound Studies To Elucidate the Effect of “Natural” Mediators on Oxidoreductase-Catalyzed Degradation of Lignocellulosic Materials

ACS symposium series (2012)

Paula Nousiainen Jussi Kontro Pekka Maijala Eva Uzan Annele Hatakka

Eco-efficient biotechnological applications are important innovations for modern industrial developments. Oxidoreductases, e.g. laccases and manganese peroxidases (MnPs), provide interesting possibilities. In many cases these enzymes require low molecular weight co-substrates to mediate oxidation. In this study, various types of lignin model compounds were investigated both as mediators and as final targets to find out their effect and potential in oxidation reactions, catalyzed by laccases and MnPs. Especially acetosyringone and methyl syringate mediated the laccase-catalyzed oxidation. These phenolics were also found to facilitate MnP-catalyzed reactions of non-phenolic compounds. Oxidation potentials, pH and chemical stability of the mediator affected the oxidation efficiency.

Acetosyringone

Degradation

Natural product

10.1021/bk-2012-1107.ch012

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Citations (5)

9th International Conference on Biotechnology in the Pulp and Paper Industry Durban, South Africa, 10-14 October 2004

Annele Hatakka Pekka Maijala Terhi K. Hakala Kristiina Hildén Leena Hauhio

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Application of some seismic data processing methods to ground penetrating radar data

Pekka Maijala University of Oulu

Digitally recorded ground penetrating radar (GPR) data resembles reflection seismic data. Both GPR and the reflection seismic method rely on an impulse-type wave signal, whose length in wavelengths varies with the source. Wave packets are sent into the ground by radio-frequency antennas in the case of GPR and by acoustic sources in the case of reflection seismics. The waves are propagated, attenuated and reflected within the studied material.

Ground-Penetrating Radar

Data Processing

10.3997/2214-4609-pdb.303.14

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Citations (15)

Novel thermotolerant laccases produced by the white-rot fungus Physisporinus rivulosus

Applied Microbiology and Biotechnology (2007)

Kristiina Hildén Terhi K. Hakala Pekka Maijala Taina Lundell Annele Hatakka

Guaiacol

Sawdust

ABTS

Biotransformation

10.1007/s00253-007-1155-x

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Citations (72)

ENHANCED METHANE PRODUCTION AND ENZYMATIC DISASSEMBLY OF ANNUAL CROPS BY ENSILING

Journal of Biotechnology (2010)

Annukka Pakarinen Seija Jaakkola Pekka Maijala Liisa Viikari

10.1016/j.jbiotec.2010.10.060

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Innovaatiot liiketoiminnan uudistajana: Yrityskokemuksia eri toimialoilta ja erikokoisista yrityksistä toimintamallin kehittämiseksi: Väliraportti II

Jaakko Paasi Pasi Valkokari Pekka Maijala Sirra Toivonen Tuija Luoma

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Tuotteen turvallisuuden arviointikriteerit

Pekka Maijala Anna Dańska

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Differential regulation of manganese peroxidases and characterization of two variable MnP encoding genes in the white-rot fungus Physisporinus rivulosus

Applied Microbiology and Biotechnology (2006)

Terhi K. Hakala Kristiina Hildén Pekka Maijala Cia Olsson Annele Hatakka

Manganese peroxidase

Sawdust

Lignin peroxidase

10.1007/s00253-006-0541-0

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Citations (58)

Enzymatic pretreatment of seed flax- and polylactide-commingled nonwovens for composite processing

Journal of Thermoplastic Composite Materials (2013)

Tiina Hallila Pekka Maijala Jyrki Vuorinen Liisa Viikari

Commingled composites of polylactide (PLA) and seed flax fibers were prepared by carding and needle pressing of PLA and flax fibers to nonwoven mats followed by compression molding. The nonwoven mats were treated using two different types of cellulolytic enzymes, namely cellobiohydrolases and endoglucanases. Both the enzymes changed the properties of the seed flax fibers surface. The resulting composites were studied using scanning electron microscopy, mechanical testing and thermal analysis. The effects of these modifications were minor on the thermal and mechanical properties measured. However, in the scanning electron micrographs, a difference in the fiber pull-out behavior of the untreated and treated fibers was observed. Treated seed flax fibers showed an improvement in adhesion with PLA.

Carding

Compression molding

Molding (decorative)

10.1177/0892705712473623

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Citations (5)