Petra Bencúrová

Masaryk University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Milan Brázdil

Central European Institute of Technology

ŠP

Šárka Pospı́šilová

Central European Institute of Technology

Jiří Baloun

Institute of Rheumatology

Hana Kubová

Czech Academy of Sciences

Martin Pail

Central European Institute of Technology – Masaryk University

Jakub Hynšt

Central European Institute of Technology

Markéta Hermanová

Masaryk University

Marek Mráz

University Hospital Brno

Klára Marečková

Central European Institute of Technology

Kateřina Musilová

Universidad de Navarra

Cooperative Institutions

Masaryk University

Central European Institute of Technology – Masaryk University

Central European Institute of Technology

University Hospital Brno

St. Anne's University Hospital Brno

Charles University

University of Minnesota

University of Eastern Finland

Finland University

Resonance Research (United States)

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis

Petra Bencúrová Jiří Baloun Kateřina Musilová Karol Pál Nikola Tom

Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects. Differential expression levels in mTLE+ HS patients were identified also in the case of 10 miRNA species never considered to play a role in epilepsy before. Conclusion: Analytical approach combining NGS with miQPCR used for the first time for analysis of human epileptic foci led to reliable identification of miRNA with altered expression in mTLE +HS patients.

Hippocampal sclerosis

Source

Cite

Citations (0)

miRNA screening of infantile and adult rat hippocampal tissues in acute phase of epilepsy

Jiří Baloun Petra Bencúrová Jakub Hynšt Hana Kubová Milan Brázdil

Source

Cite

Citations (0)

Cortisol in deciduous tooth tissues: A potential metric for assessing stress exposure in archaeological and living populations

International Journal of Paleopathology (2023)

Leslie Quade Miroslav Králík Petra Bencúrová Erin C. Dunn

10.1016/j.ijpp.2023.08.001

Cite

Citations (2)

Perinatal stress and human hippocampal volume: Findings from typically developing young adults

Scientific Reports (2018)

Klára Marečková Radek Mareček Petra Bencúrová Jana Klánová Ladislav Dušek

The main objective of this study was to investigate the impact of prenatal and early postnatal stress on hippocampal volume in young adulthood. In sharp contrast to numerous results in animal models, our data from a neuroimaging follow-up (n = 131) of a community-based birth cohort from the Czech Republic (European Longitudinal Study of Pregnancy and Childhood) showed that in typically developing young adults, hippocampal volume was not associated with birth weight, stressful life events during the prenatal or early postnatal period, or dysregulated mood and wellbeing in the mother during the early postnatal period. Interestingly, mother's anxiety/co-dependence during the first weeks after birth did show long-lasting effects on the hippocampal volume in young adult offspring irrespective of sex. Further analyses revealed that these effects were subfield-specific; present in CA1, CA2/3, CA4, GC-DG, subiculum, molecular layer, and HATA, hippocampal subfields identified by translational research as most stress- and glucocorticoid-sensitive, but not in the remaining subfields. Our findings provide evidence that the type of early stress is critical when studying its effects on the human brain.

Prenatal Stress

Subiculum

10.1038/s41598-018-23046-6

Cite

Citations (29)

23-Prenatal stress, mood, and gray matter volume in young adulthood

Clinical Neurophysiology (2018)

Klára Marečková Anja Klasnja Petra Bencúrová Lenka Andrýsková Milan Brázdil

Prenatal Stress

10.1016/j.clinph.2018.01.043

Cite

Citations (2)

Dynamic miRNA changes during the process of epileptogenesis in an infantile and adult-onset model

Scientific Reports (2021)

Petra Bencúrová Jiří Baloun Jakub Hynšt Jan Oppelt Hana Kubová

Abstract Temporal lobe epilepsy (TLE) is the most common epilepsy type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to the early onset of the disease. Although the miRNA involvement in TLE is widely studied, the relationship between the onset-age and miRNA expression has not been addressed. Here, we investigated the miRNA profile of infantile and adult-onset TLE in rats combining sequencing and PCR. Since miRNA expression changes with the disease progression, we scrutinized miRNA dynamics across three stages: acute, latent, and chronic. We report that infantile-onset TLE leads to changes in the expression of fewer miRNAs across these stages. Interestingly, the miRNA profile in the acute stage of infantile-onset TLE overlaps in dysregulation of miR-132-5p, -205, and -211-3p with the chronic stage of the disease starting in adulthood. The analysis of putative targets linked the majority of dysregulated miRNAs with pathways involved in epilepsy. Our profiling uncovered miRNA expression characteristic for infantile and adulthood-onset epileptogenesis, suggesting the distinct biology underlying TLE in the onset age-dependent matter. Our results indicate the necessity of addressing the onset age as an important parameter in future epilepsy research.

10.1038/s41598-021-89084-9

Cite

Citations (17)

Detection of circulating miRNAs in the blood plasma of patientswith mTLE+HS

Jiří Baloun Petra Bencúrová Martin Pail Milan Brázdil Šárka Pospı́šilová

Source

Cite

Citations (0)

MicroRNA and mesial temporal lobe epilepsy with hippocampal sclerosis: Whole miRNome profiling of human hippocampus

Epilepsia (2017)

Petra Bencúrová Jiří Baloun Kateřina Musilová Lenka Radová Boris Tichý

Mesial temporal lobe epilepsy (mTLE) is a severe neurological disorder characterized by recurrent seizures. mTLE is frequently accompanied by neurodegeneration in the hippocampus resulting in hippocampal sclerosis (HS), the most common morphological correlate of drug resistance in mTLE patients. Incomplete knowledge of pathological changes in mTLE+HS complicates its therapy. The pathological mechanism underlying mTLE+HS may involve abnormal gene expression regulation, including posttranscriptional networks involving microRNAs (miRNAs). miRNA expression deregulation has been reported in various disorders, including epilepsy. However, the miRNA profile of mTLE+HS is not completely known and needs to be addressed.Here, we have focused on hippocampal miRNA profiling in 33 mTLE+HS patients and nine postmortem controls to reveal abnormally expressed miRNAs. In this study, we significantly reduced technology-related bias (the most common source of false positivity in miRNA profiling data) by combining two different miRNA profiling methods, namely next generation sequencing and miRNA-specific quantitative real-time polymerase chain reaction.These methods combined have identified and validated 20 miRNAs with altered expression in the human epileptic hippocampus; 19 miRNAs were up-regulated and one down-regulated in mTLE+HS patients. Nine of these miRNAs have not been previously associated with epilepsy, and 19 aberrantly expressed miRNAs potentially regulate the targets and pathways linked with epilepsy (such as potassium channels, γ-aminobutyric acid, neurotrophin signaling, and axon guidance).This study extends current knowledge of miRNA-mediated gene expression regulation in mTLE+HS by identifying miRNAs with altered expression in mTLE+HS, including nine novel abnormally expressed miRNAs and their putative targets. These observations further encourage the potential of microRNA-based biomarkers or therapies.

Hippocampal sclerosis

10.1111/epi.13870

Cite

Citations (45)

mTLE v dospělosti a dětství – rozdíly v miRNA profilech dospělých a novorozených potkanů

Petra Bencúrová Jiří Baloun Jakub Hynšt Šárka Pospı́šilová Hana Kubová

Uvod: Pocatek epilepsie se casto objevuje v ranem dětstvi, kdy je mozek nachylnějsi ke vzniku zachvatů. Epilepsie s pocatkem v dětstvi se lisi od těch ziskaných v dospělosti na urovni klinicke, morfologicke i behavioralni. Cil: Hlavnim cilem teto studie bylo identifi kovat rozdily v expresi microRNA (miRNA/miR) v hipokampalnich tkanich potkanů s epileptogenezi zapocatou epileptickým statem (SE), který byl indukovan pomoci LiCl/pilokarpinu v dospělosti (P60) a novorozeneckem obdobi (P12). Metodika: U potkanů byl LiCl/pilokarpinem vyvolan status epilepticus v P60 (n = 10) nebo P12 (n = 10). Zviřata stejneho věku byla použita jako kontroly (n = 10 v každe věkove skupině). Hipokampalni tkaně byly odebrany 24 hod nebo 3 měsice po SE. Expresni profi ly miRNA byly stanoveny v jednotlivých vzorcich pomoci sekvenovani kratkých RNA. Výsledky: Sekvenovani ukazalo, že změny v regulaci ně kte rých miRNA byly shodne jak u mlaďat, tak u dospělých potkanů v akutni fazi. Naproti tomu 9 miRNA, ktere měly změněnou expresi v akutni fazi, vykazovaly obdobný trend i v chronicke fazi pouze u dospělých potkanů po SE. Jenom miR-155-5p měla shodně změněnou expresi u vsech zviřat po SE. Zavěr: Nase výsledky ukazuji, že vývojove stadium mozku v case vzniku epilepsie ma vliv na regulaci genove exprese, což může zaznamenat odlisnosti v patologickem procesu a nutnost upravy lecby

Source

Cite

Citations (0)

Whole miRNome Sequencing of Human Epileptic Brain Tissue

Petra Bencúrová Jiří Baloun Kateřina Musilová Šárka Pospı́šilová Markéta Hermanová

Mesial temporal lobe epilepsy (mTLE) is a chronic neurological disorder characterized by recurrent seizures. Accumulating evidence of microRNAs´ (miRNAs) potential involvement in pathogenic mechanisms underlying mTLE is emerging. The function of microRNAs in chronic mTLE is still poorly understood partially due to the lack of a clear cut miRNA expression profile of epileptic foci. In the present study, we performed the whole miRNome profiling analysis of the human brain tissue employing novel approach combining next generation sequencing (NGS) with an advanced qPCR technique, miQPCR. miRNA profiles of the hippocampus of autopsy controls and mTLE patients were compared, and analysis revealed differential miRNA expression of 24 miRNAs consistent with published data as well as several miRNA species never considered to play a role in epilepsy before. In conclusion, NGS exposed a group of miRNAs manifesting invariable expression across all samples out of which stable expression of four miRNAs was validated by combined methods for future utilization as negative controls or normalizers.

Source

Cite

Citations (0)