Elizabeth Xisto Souto

Dr. Euryclides Hospital de Jesus Zerbini

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Fabíola Attié de Castro

Universidade de São Paulo

Natália de Souza Nunes

Universidade de São Paulo

Raquel Tognon

Universidade Federal de Juiz de Fora

Dimas Tadeu Covas

Instituto Butantan

Belinda Pinto Simões

Universidade do Estado do Amazonas

Juçara Gastaldi Cominal

Universidade de Ribeirão Preto

Maira da Costa Cacemiro

Universidade de Ribeirão Preto

Maria Gabriela Berzoti-Coelho

Universidade de São Paulo

Irene Lorand‐Metze

Universidade Estadual de Campinas (UNICAMP)

Lorena Lôbo Figueiredo-Pontes

Universidade de São Paulo

Cooperative Institutions

Universidade de São Paulo

Universidade de Ribeirão Preto

Clinics Hospital of Ribeirão Preto

Dr. Euryclides Hospital de Jesus Zerbini

Universidade Federal de São Paulo

Hospital Israelita Albert Einstein

Instituto de Ciências Farmacêuticas

Fundação de Amparo à Pesquisa do Estado de São Paulo

Universidade Estadual de Campinas (UNICAMP)

Hospital São Paulo

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Updating recommendations of the Brazilian Group of Flow Cytometry (GBCFLUX) for diagnosis of acute leukemias using four-color flow cytometry panels

Hematology Transfusion and Cell Therapy (2021)

Miriam Perlingeiro Beltrame Elizabeth Xisto Souto Mihoko Yamamoto Felipe Magalhães Furtado Elaine Sobral da Costa

Flow cytometry has become an increasingly important tool in the clinical laboratory for the diagnosis and monitoring of many hematopoietic neoplasms. This method is ideal for immunophenotypic identification of cellular subpopulations in complex samples, such as bone marrow and peripheral blood. In general, 4-color panels appear to be adequate, depending on the assay. In acute leukemias (ALs), it is necessary identify and characterize the population of abnormal cells in order to recognize the compromised lineage and classify leukemia according to the WHO criteria. Although the use of eight- to ten-color immunophenotyping panels is wellestablished, many laboratories do not have access to this technology.In 2015, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) proposed antibody panels designed to allow the precise diagnosis and characterization of AL within available resources. As many Brazilian flow cytometry laboratories use four-color immunophenotyping, the GBCFLUX has updated that document, according to current leukemia knowledge and after a forum of discussion and validation of antibody panels.Recommendations for morphological analysis of bone marrow smears and performing screening panel for lineage (s) identification of AL were maintained from the previous publication. The lineage-oriented proposed panels for B and T cell acute lymphoblastic leukemia (ALL) and for acute myeloid leukemia (AML) were constructed for an appropriate leukemia classification.Three levels of recommendations (i.e., mandatory, recommended, and optional) were established to enable an accurate diagnosis with some flexibility, considering local laboratory resources and patient-specific needs.

Immunophenotyping

Cytometry

10.1016/j.htct.2021.04.001

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Citations (3)

Hippo pathway-related genes expression is deregulated in myeloproliferative neoplasms

Medical Oncology (2022)

Maira da Costa Cacemiro Juçara Gastaldi Cominal Luíz Miguel Pereira Maria Gabriela Berzoti-Coelho Giovana Michelassi Berbel

Hippo signaling pathway

Pathogenesis

10.1007/s12032-022-01696-x

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Citations (2)

Tratamento de suporte e quelação de ferro em pacientes com síndromes mielodisplásicas

Revista Brasileira de Hematologia e Hemoterapia (2006)

Elizabeth Xisto Souto

As síndromes mielodisplásicas (SMD) são um grupo heterogêneo de distúrbios hematológicos que ocorrem mais freqüentemente em pacientes idosos e que cursa, na maioria dos casos, com anemia crônica dependente de transfusão de hemoderivados. Conseqüentemente, muitos destes pacientes passam a apresentar sobrecarga de ferro, que pode levar a danos teciduais graves. Ambas as terapias, transfusional e de quelação de ferro, quando indicadas, são importantes para manter a sobrevida e a qualidade de vida destes pacientes. A terapia de quelação de ferro está indicada especialmente nos subtipos de SMD com melhor prognóstico e sobrevida longa o suficiente para o desenvolvimento de sobrecarga de ferro com relevância clínica. A terapia de quelação de ferro apresenta algumas limitações relacionadas à necessidade de longo tempo de infusão da deferoxamina, da dificuldade de adesão pelo paciente, bem como da aquisição da bomba de infusão. O uso da deferiprona, que é um quelante oral de ferro, está contra-indicado neste grupo de pacientes, pelo risco de neutropenia e agranulocitose. O deferasirox é um novo quelante oral de ferro em estudo e que poderá, no futuro, ser uma opção adequada para os pacientes com SMD e sobrecarga de ferro. Novos estudos em pacientes com síndromes mielodisplásicas são necessários para melhor estabelecer critérios de diagnóstico da sobrecarga de ferro, bem com da terapia de quelação neste grupo.

10.1590/s1516-84842006000300011

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Citations (1)

Mir-16 and 21 Deregulation Are Linked to Anti-Apoptotic Bcl-2 Gene Expression in Polycythemia Vera

Blood (2012)

Natália de Souza Nunes Raquel Tognon-Ribeiro Lívia Gonzaga Moura Simone Kashima Dimas Tadeu Covas

10.1182/blood.v120.21.5057.5057

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Citations (0)

Myelodysplastic syndromes (MDS): prognostic factors and scoring systems

Sao Paulo Medical Journal (1997)

Elizabeth Xisto Souto Maria de Lourdes Lopes Ferrari Chauffaile José Eduardo Cajado Moncau L Niéro‐Melo Gisele Wally Braga

OBJECTIVE: To evaluate the score systems of Cassano and Sanz and suggest a new one. DESIGN: Case series. LOCATION: Teaching hospitals: EPM UNIFESP and Faculdade de Medicina de Botucatu. PARTICIPANTS: 59 patients diagnosed from 1979 to 1992. INTERVENTION: Evaluation of clinical-laboratorial data. MEASUREMENT: Statistical comparison, uni and multivariate analysis and actuarial survival curves. RESULTS: Cassano's system divided the patients into high and low risk (p=0.0966) while, Sanz's gave high, intermediate and low risk (p=0.0108). The univariate analysis showed hemoglobin, WBC count, E/M ratio, liver size and blast percentage in BM as statistically significant. The multivariate analysis showed blast percentage in BM (p=0.004) and Hb (p=0.050) as significant. Our system, considering the multivariate analysis data, divided the patients into high, intermediate and low risk (p=0.0038). CONCLUSIONS: Sanz's system was more functional than Cassano's, while ours showed predictive survival value and ease of use in clinical practice.

10.1590/s1516-31801997000500005

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Citations (3)

Alteraçöes citogenética como fatores prognósticos em leucemias mielóides agudas

Revista da Associação Médica Brasileira (1996)

M.L.L.F. Chauffaille Masakuni Yamamoto José Eduardo Cajado Moncau Gisele Wally Braga Elizabeth Xisto Souto

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Hippo Pathway-related Genes Expression Is Deregulated in Myeloproliferative Neoplasms

Research Square (Research Square) (2021)

Maira da Costa Cacemiro Juçara Gastaldi Cominal Luíz Miguel Pereira Maria Gabriela Berzoti-Coelho Giovana Michelassi Berbel

Abstract Myeloproliferative neoplasms (MPN) are hematological disorders characterized by increased proliferation of precursor and mature myeloid cells. MPN patients may present driver mutations in JAK2 , MPL , and CALR genes, which are essential to describe the molecular mechanisms of MPN pathogenesis. Despite all the new knowledge on MPN pathogenesis, many questions remain to be answered to develop effective therapies to cure MPN or impair its progression to acute myeloid leukemia. The present study examined the expression levels of the Hippo signaling pathway members in patients with polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), as well as the role that they play in disease pathogenesis. The Hippo pathway is a tumor suppressor pathway that participates in the regulation of cell proliferation, differentiation, and death. Our main findings were: (i) expression of tumor suppressor genes from Hippo pathway were downregulated and seemed to be associated with cell resistance to apoptosis and increased proliferation rate; and (ii) Hippo pathway-related gene expression was associated with mutation status in ET and PMF patients. Therefore, the decreased expression of Hippo pathway-related genes may contribute to the malignant phenotype, apoptosis resistance, and cell proliferation in MPN pathogenesis.

Hippo signaling pathway

Pathogenesis

10.21203/rs.3.rs-1107475/v1

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The expression of Death Inducer-Obliterator (DIDO) variants in Myeloproliferative Neoplasms

Blood Cells Molecules and Diseases (2016)

Maria Gabriela Berzoti-Coelho Aline Fernanda Ferreira Natália de Souza Nunes Mariana Tomazini Pinto Maurício Cristiano Rocha Júnior

Myeloproliferative neoplasm

Pathogenesis

DIDO

Janus kinase 2

10.1016/j.bcmd.2016.03.008

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Citations (8)

PO026 A rare case of pure erythroid leukemia in childhood, with cytogenetic abnormalities including 7 monosomy and 47, XXY. Probably a evolution of MDS

Leukemia Research (2007)

Elizabeth Xisto Souto S.C.M. Barbosa E.A.S. Tito SS Ioguy I.C. Cruz

Monosomy

Childhood leukemia

10.1016/s0145-2126(07)70256-4

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First proposed panels on acute leukemia for four-color immunophenotyping by flow cytometry from the Brazilian group of flow cytometry-GBCFLUX

Cytometry Part B Clinical Cytometry (2014)

Maura R. V. Ikoma Alex Freire Sandes Leandro S. Thiago Geraldo Barroso Cavalcanti Irene Lorand‐Metze

Multiparameter flow cytometry is a highly sensitive, fast, and specific diagnostic technology with a wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories are equipped with four-color flow cytometer facilities. Based on this fact, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) for standardization of clinical flow cytometry has proposed an antibody panel designed to allow precise diagnosis and characterization of acute leukemia (AL) within resource-restricted areas. Morphological analysis of bone marrow smears, together with the screening panel, is mandatory for the primary identification of AL. The disease-oriented panels proposed here are divided into three levels of recommendations (mandatory, recommendable, and optional) in order to provide an accurate final diagnosis, as well as allow some degree of flexibility based on available local resources and patient-specific needs. The proposed panels will be subsequently validated in an interlaboratory study to evaluate its effectiveness on the diagnosis and classification of AL. (Assoc editor comm. 2).

Immunophenotyping

Cytometry

10.1002/cyto.b.21175

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Citations (11)