Xiaocai Cui

Huazhong Agricultural University

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Yun Zhou

Huazhong Agricultural University

Beibei Wu

Huazhong Agricultural University

Qingqing Ren

Nanjing University of Aeronautics and Astronautics

Tian Ren

Huazhong Agricultural University

Ying Liu

Ningbo University

Ziqi Wang

Henan Normal University

Ting Ran

Huazhong Agricultural University

Siqing Xia

State Key Laboratory of Pollution Control and Resource Reuse

Bruce E. Rittmann

Arizona State University

Ying Liu

Queensland University of Technology

Cooperative Institutions

Huazhong Agricultural University

Chinese Academy of Sciences

Tongji University

Harbin Institute of Technology

Shanghai Institute of Pollution Control and Ecological Security

Sichuan University

Shenzhen University

Soochow University

State Key Laboratory of Pollution Control and Resource Reuse

Arizona State University

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Assessment and optimization of the oxygen based membrane biofilm reactor as a novel technology for source-diverted greywater treatment

The Science of The Total Environment (2021)

Qingqing Ren Xiaocai Cui Xingtao Zuo Jiajie He Yun Zhou

Greywater

Central composite design

10.1016/j.scitotenv.2021.151763

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Citations (10)

Performance and mechanisms of greywater treatment in a bio-enhanced granular-activated carbon dynamic biofilm reactor

npj Clean Water (2022)

Ziqi Wang Ying Yang Wanchen Xiang Beibei Wu Xiaocai Cui

Abstract The potential of source-diverted graywater reuse mainly relies on the efficiency and cost of graywater treatment technology. Oxygen (O 2 ) supply and utilization rate directly determine the energy consumption and pollutants removal rate in the biological graywater treatment. This study developed a gravity flow self-supplying O 2 and easy-to-maintain bio-enhanced granular-activated carbon dynamic biofilm reactor (BhGAC-DBfR) for on-site graywater treatment. Results showed that increasing of saturated/unsaturated ratio led to the continuous growth of biomass on GAC surface. Division of saturated and unsaturated zones favors the formation of aerobic-anoxic-anaerobic biofilm in the reactor. A saturated/unsaturated ratio of 1:1.1 achieved the maximum removal rate of chemical oxygen demand (COD), linear alkylbenzene sulfonates (LAS), ammonia nitrogen, and total nitrogen at 98.3%, 99.4%, 99.8%, and 83.5%, respectively. Key is that adsorption and biodegradation play important and distinct roles in the quick uptake and continuous removal of both organics and N in the system. The related genus and enzymes functional for LAS mineralization, deamination of organic N, ammonium oxidation, and nitrate respiration enabled the efficient and simultaneous removal of organics and N in the BhGAC-DBfR. This study offers a promising engineering alternative technology with great potential to achieve efficient and low-energy-input graywater treatment.

Greywater

Biochemical oxygen demand

10.1038/s41545-022-00198-7

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Citations (13)

Mass Flow and Metabolic Pathway of Nonaeration Greywater Treatment in an Oxygenic Microalgal–Bacterial Biofilm

Environmental Science & Technology (2023)

Yun Zhou Beibei Wu Xiaocai Cui Tian Ren Ting Ran

A symbiotic microalgal–bacterial biofilm can enable efficient carbon (C) and nitrogen (N) removal during aeration-free wastewater treatment. However, the contributions of microalgae and bacteria to C and N removal remain unexplored. Here, we developed a baffled oxygenic microalgal–bacterial biofilm reactor (MBBfR) for the nonaerated treatment of greywater. A hydraulic retention time (HRT) of 6 h gave the highest biomass concentration and biofilm thickness as well as the maximum removal of chemical oxygen demand (94.8%), linear alkylbenzenesulfonates (LAS, 99.7%), and total nitrogen (97.4%). An HRT of 4 h caused a decline in all of the performance metrics due to LAS biotoxicity. Most of C (92.6%) and N (95.7%) removals were ultimately associated with newly synthesized biomass, with only minor fractions transformed into CO2 (2.2%) and N2 (1.7%) on the function of multifarious-related enzymes in the symbiotic biofilm. Specifically, microalgae photosynthesis contributed to the removal of C and N at 75.3 and 79.0%, respectively, which accounted for 17.3% (C) and 16.7% (N) by bacteria assimilation. Oxygen produced by microalgae favored the efficient organics mineralization and CO2 supply by bacteria. The symbiotic biofilm system achieved stable and efficient removal of C and N during greywater treatment, thus providing a novel technology to achieve low-energy-input wastewater treatment, reuse, and resource recovery.

Greywater

Hydraulic retention time

Photobioreactor

10.1021/acs.est.3c06049

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Citations (11)

Microbial niches and dynamics of antibiotic resistance genes in a bio-enhanced granular-activated carbon biofilm treating greywater

Chemosphere (2023)

Ting Wei Ziqi Wang Ying Yang Wanchen Xiang Ying Liu

Greywater

Nitrospira

Sphingomonas

Methanobacterium

10.1016/j.chemosphere.2023.138774

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Citations (7)

Removal kinetics of linear alkylbenzene sulfonate in a batch-operated oxygen based membrane biofilm reactor treating greywater: Quantitative differentiation of adsorption and biodegradation

The Science of The Total Environment (2021)

Xiaocai Cui Qingqing Ren Jie Zhang Yun Zhou

Greywater

Extracellular polymeric substance

Linear alkylbenzene

Biochemical oxygen demand

10.1016/j.scitotenv.2021.150523

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Citations (21)

pH-dependent microbial niches succession and antibiotic resistance genes distribution in an oxygen-based membrane biofilm reactor treating greywater

Environmental Research (2022)

Beibei Wu Qingqing Ren Libo Xia Ying Liu Xiaocai Cui

Extracellular polymeric substance

10.1016/j.envres.2022.114725

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Citations (15)

Sensitive and Accurate Differentiation of Extracellular Polymeric Substance Hydrolysis Using Flow Cytometry during Biofilm Extracellular Polymeric Substance Extraction

ACS ES&T Water (2023)

Xiaocai Cui Tian Ren Qingqing Ren Beibei Wu Yun Zhou

Extracellular polymeric substances (EPS) affect the formation, properties, and function of biofilms. Understanding the roles of EPS in biofilm-related processes depends on reliable extraction and evaluation methods. However, differentiating true EPS from intracellular organic polymers released by cell lysis has proven to be difficult during EPS extraction. In this study, thermal and cation exchange resin (CER) treatments were used to extract biofilm EPS. SYTOX Green (SG) dye was used to stain nucleic acid (NA), and the enhanced fluorescence signal (FS) from the NA-SG complex was sensitively recorded by flow cytometry (FC). Results showed that thermal extraction could achieve effective EPS release, but a higher temperature and longer time caused a significant cell activity decline, protein denaturation, and cell lysis. In contrast, CER extraction had no effects on cell activity and organics denaturation, and it only caused minor cell lysis at a high CER dose. Combining all the results indicates that CER extraction using 70 g-CER/g-VSS for 2 h provided reliable and efficient extraction of biofilm EPS: high EPS extraction efficiency with minimal EPS denaturation, cell lysis, and loss of cell activity. FC combined with SG staining should be applicable for evaluating the effectiveness of EPS extraction from any microbial aggregates.

Extracellular polymeric substance

Denaturation (fissile materials)

10.1021/acsestwater.2c00502

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Citations (3)

Biofilm bioactivity affects nitrogen metabolism in a push-flow microalgae-bacteria biofilm reactor during aeration-free greywater treatment

Water Research (2023)

Beibei Wu Ting Ran Sibei Liu Qian Li Xiaocai Cui

Greywater

10.1016/j.watres.2023.120461

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Citations (30)

Microbial Niches and Dynamics of Antibiotic Resistance Genes in a Bio-Enhanced Granular-Activated Carbon Biofilm Treating Greywater

SSRN Electronic Journal (2022)

Ting Wei Ziqi Wang Ying Yang Wanchen Xiang Ying Liu

Accumulation and transmission of antibiotic resistance genes (ARGs) in greywater treatment systems present risks for its reuse. In this study, a gravity flow self-supplying oxygen (O2) bio-enhanced granular activated carbon dynamic biofilm reactor (BhGAC-DBfR) was developed to treat greywater. Maximum removal efficiencies were achieved at saturated/unsaturated ratios (RSt/Ust) of 1:1.1 for chemical oxygen demand (COD, 97.6 ± 1.5%), linear alkylbenzene sulfonates (LAS) (99.2 ± 0.5%), NH+ 4-N (99.3 ± 0.7%) and total nitrogen (TN, 85.3 ± 3.2%). Microbial communities were significantly different at various RSt/Ust and reactor positions (P < 0.05). The unsaturated zone with low RSt/Ust showed more abundant microorganisms than the saturated zone with high RSt/Ust. The reactor-top community was predominant by aerobic nitrification (Nitrospira) and LAS biodegradation (Pseudomonas, Rhodobacter and Hydrogenophaga) related genera; but reactor-bottom community was predominant by anaerobic denitrification and organics removal related genera (Dechloromonas and Desulfovibrio). Most of the ARGs (e.g., intI-1, sul1, sul2 and korB) were accumulated in the biofilm, which were closely associated with microbial communities at reactor top and stratification. The saturated zone can achieve over 80% removal of the tested ARGs at all operation Phases. Results suggested that BhGAC-DBfR can block the environment dissemination of ARGs during greywater treatment.

Greywater

10.2139/ssrn.4224172

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Citations (0)

Urea interfacial polymerization-based ultra-permeable polyamide composite membrane for efficient piggery wastewater purification

Chemical Engineering Journal (2024)

Lingfang Huang Xiaocai Cui Huimin Dang Yizhong Huang Quan Li

Interfacial polymerization

10.1016/j.cej.2024.158376

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Citations (0)