Genetic and environmental factors are known to play a role in the pathogenesis of ankylosing spondylitis (AS) which is the most severe subgroup of patients of spondyloarthritis. Killer Cell Immunoglobulin-like receptors (KIR) are the molecules regulating the cytotoxic response of Naturel Killer (NK) cells. KIR genes have polymorphic structures and comprise a high difference between the populations. KIR genes have been shown to be associated with the pathogenesis of several autoimmune and inflammatory diseases.

Objectives

The relationship between the KIR genes and AS predisposition was already shown in previous studies in different populations. In this particular study, we aimed to assess the relationship of the KIR genes in the develepoment of AS in a group of Turkish patients.

Methods

Study includes 68 patients with AS (31 female, 37 male, mean age: 38.27) and 75 healthy subjects (34 women, 41 men, mean age: 38.6) as a control group. Typing of sixteen different KIR genes was performed by the method of Spesific Sequence Oligonucleotide Probes (SSOP). The distribution of KIR genotypes were obtained from the data base of “www.allelefrequencies.net”. The rates of the KIR genes of the both groups were found by direct counting method and the comparision of the groups is made by Fisher9s Exact test.

Results

The frame genes KIR2DL4, 3DL2, 3DL3 and the 3DP1 were present in all patients and controls. KIR3DS1 which is one of the activating KIR genes, was found to be significantly higher in the AS cases (39.7%) than that of the control group (16%) (p=0.002), (Figure 1). In addition, there was no significant difference between AA and Bx genotypes in the patient and control groups. In the meta-analysis published by Zuo and colleagues in 2014; seven different studies conducted in Iran, Chinese, Spanish, English, Russian and Polish populations between the years of 2009-2012 were examined and it was reported that several KIR genes such as KIR2DL1, KIR2DS4, KIR2DS5 and KIR3DS1 were found to be associated with AS susceptibility. This is the first study from Turkey about the relationship between AS and KIR genes. We found KIR3DS1 gene significantly higher in Turkish AS patients as similar to the results of this meta-analysis study.

Conclusions

These results show that KIR3DS1 gene activating NK cell cytotoxicity may trigger AS development and suggesting that it is related with the disease predisposition. Our result is compatible with other Caucasian populations.

References

Yu-Lian Jiao et al.: Polymorphisms of KIRs Gene and HLA-C Alleles in Patients with Ankylosing Spondylitis: Possible Association with Susceptibility to the Disease. J Clin Immunol (2008) 28:343–349 Hai-Ning Zuo et al.: Genetic variations in the KIR gene family may contribute to susceptibility to ankylosing spondylitis: a meta-analysis, Mol Biol Rep (2014) 41:5311–5319

Disclosure of Interest

None declared

Pathogenesis

10.1136/annrheumdis-2015-eular.1489

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CONTRIBUTIONS OF FLOW CROSS MATCH (FXM) RESULTS AND DONOR SPECIFIC ANTIBODY (DSA) MEASUREMENTS TO ANTIBODY MEDIATED REJECTION (AMR) IN DONOR SENSITIZED KIDNEY TRANSPLANTS

Transplantation (2010)

Ty B. Dunn Özlem Görüroğlu Öztürk H. Noreen David Maurer Robert A. Bray

Dunn, T. B.1; Ozturk, O.2; Noreen, H.3; Maurer, D.3; Bray, R.2; Gebel, H.2; Matas, A. J.4 Author Information

Donor-Specific Antibodies

10.1097/00007890-201007272-00696

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Is cystatin C an evaluative marker for right heart functions in systemic sclerosis?

International Journal of Cardiology (2016)

Oğuz Akkuş Abdi Bozkurt Didem Arslantaş Onur Kaypaklı Durmuş Yıldıray Şahin

Interventricular septum

10.1016/j.ijcard.2016.07.093

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Evaluation of Serum Irisin Levels in Patients Developing Nephropathy Due to Type 2 Diabetes Mellitus

JEURMEDS (2023)

Gülçin Dağlıoğlu Yusuf Döğüş Gamze İçen Filiz Kibar Mehtap Evran

Objective: Type 2 diabetes mellitus is a metabolic disease characterized by multi-organ involvement, mostly the renal system, as a result of impaired blood sugar regulation.Damage to the renal system can be serious and can be noticed late.Type 2 diabetes mellitus is associated with adipokine disorders, and irisin molecule, a myokine involved in glucose and lipid metabolism, has also been reported to play a role in the pathogenesis.In this study, it was aimed to compare serum irisin levels between healthy individuals and type 2 diabetes mellitus patients at different stages in terms of nephropathy and to demonstrate that irisin can be a guide in the diagnosis and treatment of the disease in earlier stages before kidney damage develops. Material and Methods:The study included 101 samples of type 2 diabetes mellitus and 28 healthy volunteers who applied to Çukurova University Balcalı Hospital.The participants in the study were divided into five groups in total.The groups consisted of the first group including healthy volunteers without a history of diabetes, and four separate groups of diabetic nephropathy patients, which were formed by considering Mogensen's staging criteria.Serum irisin levels and routine biochemical parameters were also evaluated in the groups.Chi-square and One Way Anova tests were used for statistical analysis to compare group data.Results: Serum irisin level was found to be significantly higher in group 1 (p< 0.001).Especially in group 2 (Mogensen stage 1 hyperfiltration and hypertrophy stage), serum irisin level was found to be significantly lower than the control group (p< 0.001).Hemoglobin A1c, micoralbumin/creatinine and HOMA-IR values were found to be significantly lower in group 1 (p< 0.001 for each).It was determined that irisin had a weak negative correlation between hemoglobin A1c, microalbumin/creatinine and glomerular filtration rate respectively (p= 0.001, r= -0.286), (p= 0.199, r= -0.015), (p= 0.142, r= -0.158), while it had a weak positive correlation between HOMA-IR (p= 0.008, r= 0.308). Conclusion:According to the data obtained from the study, it was thought that serum irisin levels could be used as a guide in the early diagnosis of diabetic nephropathy before the symptoms related to nephropathy appear and the glomerular filtration rate decreases.

10.5578/jeurmeds.20235048

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Comparison of Plasma Free Fatty Acids Between Hyperemesis Gravidarum and Normal Pregnancy [318]

Obstetrics and Gynecology (2015)

Mustafa Öztürk Mustafa Ulubay Özlem Görüroğlu Öztürk Hakan Aydın Uğur Keskin

OBJECTIVE: We aimed to compare the levels of plasma free fatty acids between healthy pregnant patients and those with hyperemesis gravidarum. METHODS: The study enrolled 52 patients. Of these, 26 patients known to have hyperemesis gravidarum between 7 and 12 weeks of gestation constituted the study group and 26 patients, who were healthy pregnant women in the same weeks of gestation, were recruited as the control group. Saturated fatty acids, C14, C15, C16, C18, C20, C22, C24, monounsaturated fatty acids, C18:1n9 (oleic acid), C22:1n9 (erucic acid), C24:1n9 (nervonic acid), omega 3 free acids, C22:5n3 (eicosapentaenoic acid), C22:6n3 (docosahexaenoic acid), omega 6 free acids, C18:2n6 (linoleic acid), C20:4n6 (arachidonic acid), and C20:3n6 (homogamma-linolenic acid) were evaluated by gas chromatography–flame ionization detector. RESULTS: There was no statistical significant difference between the groups on the basis of age, gestational week, or plasma level of eicosapentaenoic acid, docosahexaenoic acid, or archidonic acid. There was a statistically significant difference between the groups on the basis of plasma level of C20 (arachidic acid) and C22 (behenic acid). C20 reduced, but the level of C22 behenic acid increased in the hyperemesis gravidarum group. CONCLUSION: Hyperemesis gravidarum did not affect the plasma levels of eicosapentaenoic acid, docosahexaenoic acid, or archidonic acid, which contribute to placental and fetal neurologic development. We detected that hyperemesis gravidarum affects the plasma levels of C20 (arachidic acid) and C22 (behenic acid). To supply energy needs, plasma levels of C20 may be decreased. C22 behenic acid is a cholesterol-raising saturated fatty acid in humans. The reason for the increase of C22 in the hyperemesis group should be investigated.

Behenic acid

Hyperemesis gravidarum

10.1097/01.aog.0000463569.21952.1f

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Evaluation of plasma fibronectine level as a probable indicator for tympanosclerosis

The Journal of Laryngology & Otology (2007)

Kemal Görür Özlem Görüroğlu Öztürk G Polat Yusuf Vayısoğlu Derya Ümit Talaş

Abstract Objectives: Wound healing, epithelial regrowth and collagen synthesis are very important factors in the repair of the traumatised tympanic membrane. The aim of the present study was to determine the role of plasma fibronectine in the aetiopathogenesis of tympanosclerosis. Methods: This prospective study included 58 patients with and 49 without tympanosclerosis. No inflammation or trauma was noted in either patient group. All patients underwent otoscopic and otomicroscopic examination, and the degree of tympanosclerosis was graded from mild (stage I) to severe (stage III). Following otological examination, blood samples were taken for plasma fibronectine measurement. Results: Following otoscopic and otomicroscopic examinations, patients' tympanosclerosis was graded as follows: 18 patients were stage I; 29 were stage II; and 11 were stage III. Statistical analyses revealed that the plasma fibronectine concentrations were significantly lower in the study group compared with the control group ( p = 0.031). In addition, fibronectine levels were lowest in the patients with severest tympanosclerosis ( p = 0.0001 in each comparison). Conclusion: The results of the present study show that serum fibronectine is important in the development and severity of tympanosclerosis.

10.1017/s0022215107007827

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Serum ischemia modified albumin levels in pregnancies with neural tube defects.

PubMed (2012)

İsmail Güzelmansur Yusuf Üstün Yaprak Engin Üstün Özlem Görüroğlu Öztürk Halıl Yaman

To evaluate ischemia-modified albumin (IMA) in women who had been pregnant with a child suffering from neural tube defect.Samples from 50 women who had been pregnant with an affected child (25 spina bifida, 25 anencephaly) and 25 controls matched for age, gestational age, and body mass index were studied. We measured serum IMA by enzyme-linked immunosorbent assay.Serum IMA was significantly higher in the study group compared to normal pregnancies (p < 0.05). The area under the receiver operating curve was 0.858 for IMA (95% CI, 0.769-0.947), whereas the optimal threshold value of IMA to discriminate between affected children and controls was 0.409 (sensitivity 88%, specificity 80%). The risk for increased IMA in mothers who have conceived a fetus with neural tube defect is 24.5 times higher than in the control group (rr = 24.5, 6.9-86.9, 95% CI) (p = 0.001).This study indicates that serum IMA in women who have conceived a fetus with neural tube defect is significantly higher than that in normal pregnant women.

Anencephaly

Neural tube defect

Ischemia-modified albumin

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Early propranolol treatment ameliorates endothelial dysfunction in experimental septic lung

Advances in Clinical and Experimental Medicine (2018)

Ezgi Özyılmaz Hacer Sinem Büyüknacar Emine Kılıç Bağır Leman Sencar Özlem Görüroğlu Öztürk

Background. Recent reports have indicated an improved prognosis in sepsis with β-blocker agents; however, the underlying action mechanism is still under debate.Objectives. The aim of this study was to investigate the potential effect of propranolol on endothelial dysfunction in septic rats.Material and Methods. The cecal ligation and puncture model (CLP) was used to generate sepsis. Adult male Wistar-Albino rats were divided into 4 groups: group 1 was a sham group, group 2 received sterile saline, group 3 received 10 mg/kg of propranolol 3 days before the intervention, and group 4 received 10 mg/kg of propranolol 30 min after CLP. Six rats from each group were sacrificed 24 h postoperatively. The remaining rats were followed for survival. We have also evaluated the effects on systemic inflammation, coagulation and the lung tissue with immunohistochemical and electron microscopic evaluation.Results. Serum tumor necrosis factor alpha (TNF-α) and plasminogen activator inhibitor-1 (PAI-1) levels, as well as tissue TNF-α scores were elevated in septic rats. Electron microscopic examination of the lung tissue showed endothelial dysfunction in the sepsis group. Pretreatment significantly improved survival. Moreover, pre-treatment altered serum vascular endothelial growth factor receptor-1 (VEGFR-1) levels and post-treatment reduced serum PAI-1 and VEGFR-1 levels. In both the preand post-treatment groups, electron microscopic examination revealed improvement of the destroyed lung endothelium and showed only mild alterations in the cytoplasmic organelles, especially in the mitochondria of the endothelial cells.Conclusion. These results suggest that the improved outcome with β-blockers in sepsis may be due to the ameliorated endothelial dysfunction. Further studies focusing on the potential effect of β-blockers on the endothelium may lead to a better understanding of sepsis.

Endothelial Dysfunction

10.17219/acem/78248

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Killer Cell Immunoglobulin-Like Receptor Genes

DergiPark (Istanbul University) (2011)

Özlem Görüroğlu Öztürk

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