Leonardo Congiu

University of Padua

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Co-Authors

Elisa Boscari

CoNISMa

Lorenzo Zane

University of Padua

F. Fontana

Azienda Sanitaria Universitaria Integrata di Trieste

Ilaria A. M. Marino

University of Padua

Milvia Chicca

University of Ferrara

Massimo Lanfredi

University of Ferrara

Federica Barbisan

University of Padua

Chiara Papetti

University of Padua

N. S. Mugue

All-Russian Research Institute Fisheries and Oceanography

Tomaso Patarnello

University of Padua

Cooperative Institutions

University of Padua

CoNISMa

University of Ferrara

University of Palermo

National Research Council

International Union for Conservation of Nature

University of Bologna

Stazione Zoologica Anton Dohrn

International Union for Conservation of Nature (Bangladesh)

Centre National de la Recherche Scientifique

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Identification of Life Cycle Stages of Cyathocephalus truncatus (Cestoda: Spathebothriidea) Using Molecular Techniques

Journal of Parasitology (2002)

Bahram Sayyaf Dezfuli Silvia Capuano Leonardo Congiu

Identification

10.2307/3285466

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Citations (1)

Mediterranean Bioconstructions Along the Italian Coast

Advances in marine biology (2018)

Gianmarco Ingrosso Marco Abbiati Fabio Badalamenti Giorgio Bavestrello Genuario Belmonte

Marine ecosystem

10.1016/bs.amb.2018.05.001

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Citations (182)

Characterization of Captive Breeders to Preserve the Residual Genetic Diversity of Adriatic Sturgeon (Acipenser naccarii)

Diversity (2022)

Federica Barca Stefano Dalle Palle Luca Schiavon Chiara Samassa Giuseppe Castaldelli

Since 1996, the Adriatic sturgeon (Acipenser naccarii) has been inscribed on the IUCN Red List as “Critically Endangered and possibly extinct in the wild”. Nowadays, its survival totally depends on restocking programs conducted by releasing juveniles generated from adult breeders reared in aquaculture. Conducting accurate genetic characterizations of all individuals potentially involved in reproduction activities is therefore of primary importance to avoid inbreeding and to maximize the genetic diversity transmitted to following generations. Since all animals reared in captivity descend from a single stock of wild origin, this offers the ideal condition for carrying out relatedness analysis based on parentage allocations. In this study, we provided the most complete characterization of about 500 individuals representing the most diverse extant stock of Adriatic sturgeon. Through the analyses of mitochondrial d-loop and 15 microsatellite loci selected from 24 genotyped loci, we identified about 30 different familiar groups, updating data on breeding stocks, increasing the genetic information already available, and extending the analyses to animals never genotyped before. Given its completeness, it will represent a reference database for any future parental allocation of recaptured animals for the inclusion of all other stocks present, as well as for the development of a long-term breeding plan. The approach used has also been proven useful on individuals of unknown genealogy, allowing for the identification of family groups and thus being proven to be promising for the analysis of stocks of other tetraploid sturgeon species.

Captivity

Captive breeding

Overexploitation

10.3390/d14100829

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Citations (3)

Genetic variability of the striped venus Chamelea gallina in the northern Adriatic Sea

Fisheries Research (2018)

Chiara Papetti Luca Schiavon Massimo Milan Magnus Lucassen Jilda Alicia Caccavo

Effective population size

Population bottleneck

10.1016/j.fishres.2018.01.006

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Citations (11)

MIPs: multi-locus intron polymorphisms in species identification and population genomics

Scientific Reports (2024)

Elisa Boscari Stefano Dalle Palle Nicola Vitulo Annalisa Scapolatiello Luca Schiavon

The study of species groups in which the presence of interspecific hybridization or introgression phenomena is known or suspected involves analysing shared bi-parentally inherited molecular markers. Current methods are based on different categories of markers among which the classical microsatellites or the more recent genome wide approaches for the analyses of thousands of SNPs or hundreds of microhaplotypes through high throughput sequencing. Our approach utilizes intron-targeted amplicon sequencing to characterise multi-locus intron polymorphisms (MIPs) and assess genetic diversity. These highly variable intron regions, combined with inter-specific transferable loci, serve as powerful multiple-SNP markers potentially suitable for various applications, from species and hybrid identification to population comparisons, without prior species knowledge. We developed the first panel of MIPs highly transferable across fish genomes, effectively distinguishing between species, even those closely related, and populations with different structures. MIPs offer versatile, hypervariable nuclear markers and promise to be especially useful when multiple nuclear loci must be genotyped across different species, such as for the monitoring of interspecific hybridization. Moreover, the relatively long sequences obtained ease the development of single-locus PCR-based diagnostic markers. This method, here demonstrated in teleost fishes, can be readily applied to other taxa, unlocking a new source of genetic variation.

Introgression

Amplicon

10.1038/s41598-024-68065-8

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Citations (1)

Fluorescent in situ hybridization with rDNA probes on chromosomes of Acipenser ruthenus and Acipenser naccarii (Osteichthyes Acipenseriformes)

Genome (1999)

Francesco Fontana Massimo Lanfredi Milvia Chicca Leonardo Congiu James Tagliavini

The genes for 28S and 5S rDNA were physically mapped on the chromosomes of two sturgeon species, the sterlet (Acipenser ruthenus, 2n = 118 ± 4) and the Adriatic sturgeon (Acipenser naccarii, 2n = 248 ± 4) by fluorescent in situ hybridization. In the sterlet, the 28S rDNA was located on six chromosomes, four of which actively transcribed, while in the Adriatic sturgeon the 28S rDNA was located on a chromosome number ranging from 10 to 12, eight of which actively transcribed. The 5S rDNA was physically mapped on two chromosomes in the sterlet and on four in the Adriatic sturgeon. A more detailed characterization of the latter karyotype was obtained during this study. All these data are discussed in connection with the ploidy relationships among sturgeon species.Key words: karyotype, ploidy, FISH, 28S and 5S rDNA.

Huso

10.1139/g99-030

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Citations (26)

Spatial variability of the Po River food web and its comparison with the Danube River food web

PLoS ONE (2023)

Katalin Patonai Ferenc Jordán Giuseppe Castaldelli Leonardo Congiu Anna Gavioli

Freshwater ecosystems are experiencing unprecedented pressure globally. To address environmental challenges, systematic and comparative studies on ecosystems are needed, though mostly lacking, especially for rivers. Here, we describe the food web of the Po River (as integrated from the white literature and monitoring data), describe the three river sections using network analysis, and compare our results with the previously compiled Danube River food web. The Po River food web was taxonomically aggregated in five consecutive steps (T1-T5) and it was also analyzed using the regular equivalence (REGE) algorithm to identify structurally similar nodes in the most aggregated T5 model. In total, the two river food webs shared 30 nodes. Two network metrics (normalized degree centrality [nDC]) and normalized betweenness centrality [nBC]) were compared using Mann-Whitney tests in the two rivers. On average, the Po River nodes have larger nDC values than in the Danube, meaning that neighboring connections are better mapped. Regarding nBC, there were no significant differences between the two rivers. Finally, based on both centrality indices, Carassius auratus is the most important node in the Po River food web, whereas phytoplankton and detritus are most important in the Danube River. Using network analysis and comparative methods, it is possible to draw attention to important trophic groups and knowledge gaps, which can guide future research. These simple models for the Po River food web can pave the way for more advanced models, supporting quantitative and predictive-as well as more functional-descriptions of ecosystems.

10.1371/journal.pone.0288652

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Citations (1)

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2010–31 January 2011

Molecular Ecology Resources (2011)

Kiyokazu Agata Samer Alasaad Vera Maria Fonseca de Almeida‐Val José Antonio Álvarez‐Dios Federica Barbisan

Abstract This article documents the addition of 238 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alytes dickhilleni , Arapaima gigas , Austropotamobius italicus , Blumeria graminis f. sp. tritici , Cobitis lutheri , Dendroctonus ponderosae , Glossina morsitans morsitans , Haplophilus subterraneus , Kirengeshoma palmata , Lysimachia japonica , Macrolophus pygmaeus , Microtus cabrerae , Mytilus galloprovinciali s, Pallisentis ( Neosentis ) celatus , Pulmonaria officinalis , Salminus franciscanus , Thais chocolata and Zootoca vivipara . These loci were cross‐tested on the following species: Acanthina monodon , Alytes cisternasii , Alytes maurus , Alytes muletensis , Alytes obstetricans almogavarii , Alytes obstetricans boscai , Alytes obstetricans obstetricans , Alytes obstetricans pertinax , Cambarellus montezumae , Cambarellus zempoalensis , Chorus giganteus , Cobitis tetralineata , Glossina fuscipes fuscipes , Glossina pallidipes , Lysimachia japonica var. japonica , Lysimachia japonica var. minutissima , Orconectes virilis , Pacifastacus leniusculus , Procambarus clarkii , Salminus brasiliensis and Salminus hilarii .

10.1111/j.1755-0998.2011.03004.x

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Citations (43)

Phylogenetic relationships of the North-eastern Atlantic and Mediterranean forms of Atherina (Pisces, Atherinidae)

Molecular Phylogenetics and Evolution (2007)

Sara M. Francisco Leonardo Congiu Sérgio Stefanni Rita Castilho Alberto Brito

mtDNA control region

10.1016/j.ympev.2007.12.009

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Citations (35)

Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade

Molecular Ecology Resources (2013)

Elisa Boscari Anna Barmintseva J. M. Pujolar Phaedra Doukakis N. S. Mugue

Abstract Overexploitation of wild populations due to the high economic value of caviar has driven sturgeons to near extinction. The high prices commanded by caviar on world markets have made it a magnet for illegal and fraudulent caviar trade, often involving low‐value farmed caviar being sold as top‐quality caviar. We present a new molecular approach for the identification of pure sturgeon species and hybrids that are among the most commercialized species in E urope and N orth A merica. Our test is based on the discovery of species‐specific single nucleotide polymorphisms ( SNP s) in the ribosomal protein S7, supplemented with the Vimentin gene and the mitochondrial D‐loop. Test validations performed in 702 specimens of target and nontarget sturgeon species demonstrated a 100% identification success for Acipenser naccarii , A. fulvescens , A. stellatus , A. sinensis and A. transmontanus . In addition to species identification, our approach allows the identification of B ester and AL hybrids, two of the most economically important hybrids in the world, with 80% and 100% success, respectively. Moreover, the approach has the potential to identify many other existing sturgeon hybrids. The development of a standardized sturgeon identification tool will directly benefit trade law enforcement, providing the tools to monitor and regulate the legal trade of caviar and protect sturgeon stocks from illicit producers and traders, hence contributing to safeguarding this group of heavily threatened species.

Identification

Overexploitation

Species Identification

10.1111/1755-0998.12203

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Citations (48)